Method for extracting peanut meal polysaccharide

An extraction method and peanut meal technology, applied in the field of biochemistry, can solve the problems of polysaccharide food safety impact, strong reaction conditions, environmental pollution, etc., to avoid the destruction of polysaccharide activity and safety, and improve reaction efficiency and conversion rate , the effect of improving the extraction efficiency

Active Publication Date: 2014-06-18
山东金胜生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using strong acid or strong alkali to extract peanut polysaccharides can obtain a higher yield, but due to the strong reaction conditions, it will have a certain impact on the food safety of polysaccharides, and the waste water generated during the production process will cause certain environmental pollution, so It is necessary to develop an efficient and green method for extracting polysaccharides from peanut meal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting peanut meal polysaccharides, comprising the steps of:

[0027] (1) Pretreatment: take the raw material of hot-pressed peanut meal, grind it to 500-1000 mesh, add a 70% ethanol solution according to the ratio of solid to liquid (kg / L), and then heat it at a temperature of 30°C and a stirring speed of Under the condition of 150~260r / min, fully stir for 30min, filter with suction, and take the filter residue;

[0028] (2) Enzymatic hydrolysis of protein: take the filter residue after pretreatment, add 8 times the weight of culture medium, and prepare a suspension to facilitate the full combination of enzymes, then add neutral protease (Novozymes), the amount of enzyme added Add according to 0.3g / ml suspension, enzymolysis temperature is 25°C, pH value is 6, and enzymolysis time is 2 hours;

[0029] The culture medium is (per liter): (NH 4 ) 2 SO 4 0.3g, FeSO 4 ·7H 2 O 0.02g, MnSO 4 ·H 2 O 0.03g, MgSO 4 0.02g, KH 2 PO 4 0.1g, pH 6.0;

[...

Embodiment 2

[0035] A method for extracting peanut meal polysaccharides, comprising the steps of:

[0036] (1) Pretreatment: take the raw material of hot-pressed peanut meal, grind it to 500-1000 mesh, add a 70% ethanol solution according to the ratio of solid to liquid of 1:10, and then heat it at 40°C with a stirring speed of 150-260r / min. Under the same conditions, fully stir for 30 minutes, filter with suction, and take the filter residue;

[0037] (2) Enzymatic hydrolysis of protein: take the filter residue after pretreatment, add 10 times the weight of culture medium, and prepare a suspension to facilitate the full combination of enzymes, then add neutral protease (Novozymes), the amount of enzyme added Add according to 0.3g / ml suspension, enzymolysis temperature is 30°C, pH value is 7, and enzymolysis time is 2 hours;

[0038] The culture medium is (per liter): (NH 4 ) 2 SO 4 0.3g, FeSO 4 ·7H 2 O 0.02g, MnSO 4 ·H 2 O 0.03g, MgSO 4 0.02g, KH 2 PO 4 0.1g, pH 6.0;

[003...

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PUM

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Abstract

The invention provides a method for extracting peanut meal polysaccharide. The method comprises fives steps of pretreatment, protein enzymolysis, enzyme deactivation treatment, polysaccharide extraction from complex phosphoesterasum and separation purification, wherein complex phosphoesterasum is combination of cellulose, lysozyme, beta-glucanase and amylase. By adopting the method, protein in peanut meal polysaccharide can be effectively eliminated, the functional characteristics of polysaccharide is maintained to the maximum extent, contamination of organic solvents is avoided, the extraction rate of polysaccharide is improved, the purity of polysaccharide is improved, and a wide application prospect is achieved.

Description

[0001] Technical field The present invention belongs to the technical field of biochemistry, and in particular relates to a method for extracting peanut meal polysaccharides. Background technique [0002] my country is one of the most important major peanut producing countries in the world, with an output of more than 40% of the world's total output. According to statistics, the annual output of my country's peanuts has reached 15.22 million tons, surpassing that of soybeans (15.2 million tons), and has become the first oil crop in my country. The total output, per unit yield and export volume all rank first in the world. In addition to domestic use, a large number of peanuts in my country are exported as raw materials, and the peanut deep-processing industry is relatively weak. Taking Shandong Province, where peanut exports account for 70% of the country, as an example, there are currently 18 specialized peanut food processing plants and 28 production lines; while Japan, whos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 高冠勇陈宁
Owner 山东金胜生物科技有限公司
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