66results about How to "Mild conditions for enzymatic hydrolysis" patented technology

The invention belongs to the field of medical biology, and particularly relates to a method for extracting a konjac polypeptide extract from konjac flying powder, the konjac polypeptide extract obtained by the method and an application of the konjac polypeptide extract. In order to increase the content of konjac polypeptide in the konjac polypeptide extract, the optimized extraction technology provided by the invention adopts a complex enzyme to perform enzymolysis on the protein extraction liquid of the konjac flying powder; in the finally obtained konjac polypeptide extract, the content of konjac polypeptide is as high as 85-97%, and the molecular weight of the konjac polypeptide is 350-3500Da. Moreover, the invention also provides an application of the konjac polypeptide extract in preparing a medicine for treating diabetes and diabetic complications as well as a hypoglycemic medicinal composition containing the konjac polypeptide extract. In a word, the konjac polypeptide extract and the medicinal composition thereof provided by the invention have good prospect in medicine.

The invention discloses a method for preparing soluble kelp cellulose. The method includes the steps: firstly, preparing kelp dietary fibers from kelp residues by means of flocculation, decalcifying, bleaching, washing and the like; secondly, performing steam explosion to obtain insoluble kelp cellulose, then adding low-temperature cellulase into the insoluble kelp cellulose, and performing enzymolysis in water bath at the temperature of 25 DEG C-35 DEG C to obtain the soluble kelp cellulose with the content of 90.14+/-1.09wt% and the yield of 50-60%. When the soluble kelp cellulose is prepared, the low-temperature cellulase is used, enzymolysis is performed at the normal temperature, enzymolysis conditions are moderate, only macromolecules are sensitive to the cellulose, and macromolecules in kelp are easily decomposed while micromolecules are relatively stable and not easily further decomposed, so that the obtained soluble kelp cellulose is uniform in molecular weight and high in yield, cellulose content, water-holding capacity and expansibility. Moreover, the enzymolysis temperature is low, so that production energy consumption and operability are reduced.

The invention discloses a preparation method of a jujube residue insoluble dietary fiber powder. The method comprises the following steps: 1) adding jujube residue into water of 2-5 times the weight of the jujube residue, mixing for pulping, grinding, and adding 80-120 g of cellulase, 80-120 g of protease and 80-120 g of amylase in per ton of the jujube residue slurry for enzymolysis; 2) centrifuging the jujube residue slurry treated with enzymolysis to obtain an insoluble solid, carrying out a bleaching treatment on the insoluble solid by adding hydrogen peroxide, centrifuging, compressing, filtering and drying; and 3) extruding and pulverizing the dried insoluble solid to obtain the jujube residue insoluble dietary fiber powder. The preparation method uses cellulase, protease and amylase to carry out compound enzymatic treatment, and effectively decomposes impurities; the enzymolysis conditions are mild, and cause no pollution to the environment; besides, the extrusion is employed to improve functions such as water absorption and expansion of the jujube dietary fiber.

The invention discloses a beef flavour powder and a preparation method thereof. The beef flavour powder comprises: dust salt, white granulated sugar powder, glucose powder, gourmet powder, D- xylose, L-cysteine, enzymolysis solution, soy sauce, yeast extract, sesame oil, phosphoric acid, ethyl maltol, IMP, corn starch and beef flavor. The invention adopts the method combining microwave drying technology and compound enzyme enzymolysis technology to produce the beef flavour powder which has good taste, little environmental pollution, stable quality and high safety.

A hemicellulase is the fermented product of Bacillus pumilus hemicellulase No.A1 (CCTCC M201023) and can be used in preparing chitoligose. A process for preparing the said chitoligose includes such steps as zymolyzing chitosan with the said hemicellulase, centrifugal filtering, flocculating, vacuum concentrating, filtering, decolouring, deodouring, super filtering and classifying and drying. Its advantages are high zymolyzing efficiency and high content of active components in product.

The invention relates to a method of preparing high-content fenugreek gum from industrial grade fenugreek gum. The method is characterized in that through reflux purification, high speed centrifugation, enzymolysis, resin adsorption and drying, the high-content fenugreek gum which has a pure white color, no taste and no smell, is a transparent solution and has mannogalactan content great than or equal to 95%, protein content less than or equal to 1.0% and viscosity great than or equal to 5600mPaS is obtained.

The invention relates to a method for preparation of soybean glycoprotein from defatted soybean meal, the defatted soybean meal as a raw material is soaked with water, after heating and defoaming processing, sodium hydroxide is added for adjustment of pH value to weak alkaline, after cooling, protease is added for enzymatic hydrolysis to obtain an enzymatic hydrolysate; after enzyme inactivation, activated clay and a flocculant are added for static precipitation; supernatant is taken to obtain soybean glycoprotein clear liquid; soybean glycoprotein clear liquid is added with activated carbon for carbon removal clarification and filteration to obtain soybean glycoprotein filtered liquid, the soybean glycoprotein is obtained by concentration and spray drying of the soybean glycoprotein filtered liquid. The enzymatic hydrolysis process has the advantages of mild conditions and easy control of reaction, impurities in the solution can be removed by the flocculation impurity removal process, the clear liquid is decolored and clarified by carbon removal, and the product purity is improved. The method for preparation of soybean glycoprotein from defatted soybean meal has the advantages of advanced technology, reasonable process, and controllable operation, and is suitable for mass production.

The invention discloses a chicken flavour powder and a preparation method thereof. The chicken flavour powder comprises dust salt, white granulated sugar powder, glucose powder, gourmet powder, D- xylose, isoleucine, enzymolysis solution, soy sauce, yeast extract, sesame oil, chicken oil, ethyl maltol, IMP, corn starch and chicken flavor. The invention adopts the method combining the microwave drying technology and compound enzyme enzymolysis technology to produce the chicken flavour powder which has good taste, little environmental pollution, stable quality and high safety.

The invention provides a method for extracting peanut meal polysaccharide. The method comprises fives steps of pretreatment, protein enzymolysis, enzyme deactivation treatment, polysaccharide extraction from complex phosphoesterasum and separation purification, wherein complex phosphoesterasum is combination of cellulose, lysozyme, beta-glucanase and amylase. By adopting the method, protein in peanut meal polysaccharide can be effectively eliminated, the functional characteristics of polysaccharide is maintained to the maximum extent, contamination of organic solvents is avoided, the extraction rate of polysaccharide is improved, the purity of polysaccharide is improved, and a wide application prospect is achieved.

The invention relates to a preparation method of stolephorus commersonii immunoloregulation polypeptide. The preparation method comprises the following steps: selecting a raw material stolephorus commersonii for enzymatic hydrolysis to obtain stolephorus commersonii protein peptide, effecting the obtained stolephorus commersonii protein peptide for in-vitro cultured macrophage for screening enzymatic hydrolysis conditions, and separating and purifying the material to obtain a stolephorus commersonii immunoloregulation peptide product. The method comprises the following steps: a) cleaning a rawmaterial stolephorus commersonii to prepare slurry; b) enzymatic hydrolysis: selecting protease for performing enzymatic hydrolysis on the stolephorus commersonii slurry to prepare enzymatic hydrolysis liquid; c) ultrafiltration: adding the enzymatic hydrolysis liquid in a ultrafiltration system, using ultrafilter membranes with 10KD, 5KD, and 3KD for ultrafiltration, collecting the enzymatic hydrolysis liquid with molecular weights being greater than 10KD, 10-5KD, and 5-3KD and less than 3KD; and d) performing gel column chromatography on the enzymatic hydrolysis liquid by using a glucan gelSephadex G25 and performing buffer solution eluting, respectively collecting peaks, effecting the in-vitro cultured macrophage and screening enzymatic hydrolysis condition to obtain the correspondingpeak with biological activity, and using an anti-phase high performance liquid chromatography for purifying; and e) through purifying with the anti-phase high performance liquid chromatography and amino acid sequence detection, obtaining a stolephorus commersonii immunoloregulation peptide.

The invention relates to a method for preparing periplogenin through enzymatic hydrolysis. The method is characterized in that the method comprises the following steps: 1, taking a medicinal material Cortex Periplocae, crushing, adding 3-8 times of a 80-90% methanol solution, carrying out ultrasonic extraction, recovering methanol through concentrating an extract, adding a proper amount of water to the concentrated extract, carrying out azeotrope, and centrifuging while hot to obtain a centrifugate; 2, adjusting the pH value of the centrifugate to 4.5-5.5, adding a biological enzyme, carrying out insulation enzymatic hydrolysis for 18-32h, carrying out reduced pressure concentration on an enzymatic hydrolysate, and centrifuging to obtain a crude product; and 3, processing the crude product through a high speed counter current chromatograph, collecting a fraction, and drying the fraction to obtain periplogenin. The method of the invention, which has the advantages of low pollution and high yield, is suitable for the industrialization preparation.

The invention provides high-quality strong fragrant peanut oil and a preparation method thereof. The preparation method is characterized by comprising the following steps of cleaning of peanut kernels, baking, squeezing, separation, cooling, filtering and obtaining of strong fragrant peanut oil. The prepared peanut oil contains no aflatoxin and is high in high temperature resistance, nutrient substance ingredients are reserved and not changed, the taste is pure, the fragrance is lasting, the requirement of people for extra virgin peanut oil with original taste and flavor is met, and the application prospect is wide.

The invention provides a production method of high purity Corydalis saxicola Bunting alkaloid. The method mainly includes the steps of: 1) drying and crushing medicinal materials; 2) conducting enzymolysis on the compound enzyme composed of cellulose and pectinase; 3) using ethanol with a volume concentration of 50-90% as the extraction solvent to conduct extraction concentration; 4) carrying out centrifugal separation; 5) performing treatment with centrifugal separation to obtain crude total alkaloid; and 6) subjecting the crude total alkaloid to two-stage membrane filtration, thus obtaining a purified product. The method provided by the invention has the characteristics of simple and practicable operation, mild extraction process conditions, high extraction rate, small alkaloid loss in the separation and purification process, high recovery rate, and high product purity, and can obtain Corydalis saxicola Bunting alkaloid with a total alkaloid content of greater than 98%, thus completely meeting pharmacy requirement. Through detection of the product, the Corydalis saxicola Bunting alkaloid content is 98.6%, and the heavy metals, pesticide residues and microorganism are all accord with the national standard GB/T5009, 4789 standards.

The invention relates to the technical field of pigeon egg deep processing methods, in particular to pigeon egg white powder and a preparation method thereof. The pigeon egg white powder is obtained by the following steps: adding a compound enzyme into pigeon egg white for enzymolysis to obtain an enzymolysis product, then sequentially performing filtering, performing pasteurizing, performing spray-drying, performing dry heat sterilization, performing cooling and performing sieving on the enzymolysis product to obtain the pigeon egg white powder. The preparation method is simple in preparation process, mild in enzymolysis condition, and high in drying speed, the pigeon egg white powder is uniform and consistent in white color and luster, powdery fine particles are good in restorability, easy to absorb, short in dissolution time, good in dispersity and free of agglomeration phenomenon, and the problems that existing fresh eggs are prone to deterioration and breakage and are not beneficial to storage can be effectively solved.

The invention provides a production process of organic peanut oil. The production process is characterized by comprising the following steps: selection and cleaning of organic peanuts produced in a base, stone removal, grading, color selection, baking, squeezing, crude oil filtration, preliminary cooling, filtration aid adding, secondary cooling, precipitation and filtration to obtain finished product oil. The organic peanut oil does not contain aflatoxin, is high in high-temperature resistance, retains nutritional substance components, tastes pure, has long-lasting flavor, can meet the demand of people for original-juice and original-flavor special-grade initially squeezed peanut oil, and has a wide application prospect.

The invention discloses a technology for extracting plant oil through an aqueous enzymatic method. The technology comprises the following steps: grinding oil crops, adjusting the solid to liquid ratio to 1:15-20, adding an enzyme, hydrolyzing for 18-20h, carrying out solid-liquid separation after the above enzymatic hydrolysis ends to obtain a liquid phase oil and water mixture containing a small amount of water-soluble proteins, removing the proteins through an isoelectric precipitation method, further concentrating the liquid phase for 12h, and separating to obtain the oil. Enzymatic hydrolysis conditions are mild, and the temperature of hexane leaching for recovering a solvent after the enzymatic hydrolysis is not higher than 80DEG C, so the energy consumption is obviously lower than that in traditional methods, the performances of oil proteins are well kept, and the simultaneous utilization purpose of oil and proteins is realized; and high temperature treatment of oil is avoided, so the quality of the extracted oil is obviously improved.