The invention relates to a preparation method of stolephorus commersonii immunoloregulation polypeptide. The preparation method comprises the following steps: selecting a
raw material stolephorus commersonii for
enzymatic hydrolysis to obtain stolephorus commersonii
protein peptide, effecting the obtained stolephorus commersonii
protein peptide for in-vitro cultured macrophage for screening
enzymatic hydrolysis conditions, and separating and purifying the material to obtain a stolephorus commersonii immunoloregulation
peptide product. The method comprises the following steps: a) cleaning a rawmaterial stolephorus commersonii to prepare
slurry; b)
enzymatic hydrolysis: selecting
protease for performing enzymatic
hydrolysis on the stolephorus commersonii
slurry to prepare enzymatic
hydrolysis liquid; c)
ultrafiltration: adding the enzymatic
hydrolysis liquid in a
ultrafiltration system, using ultrafilter membranes with 10KD, 5KD, and 3KD for
ultrafiltration, collecting the enzymatic hydrolysis liquid with molecular weights being greater than 10KD, 10-5KD, and 5-3KD and less than 3KD; and d) performing gel
column chromatography on the enzymatic hydrolysis liquid by using a
glucan gelSephadex G25 and performing
buffer solution eluting, respectively collecting peaks, effecting the in-vitro cultured macrophage and screening enzymatic hydrolysis condition to obtain the correspondingpeak with
biological activity, and using an anti-phase
high performance liquid chromatography for purifying; and e) through purifying with the anti-phase
high performance liquid chromatography and
amino acid sequence detection, obtaining a stolephorus commersonii immunoloregulation peptide.