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Antihypertensive peptide derived from adductor muscle of mytiloida

A thick-shelled mussel and antihypertensive peptide technology, applied in the field of antihypertensive peptides, can solve the problems of acid-base wastewater, unfriendly environment, and enzymatic hydrolysis efficiency to be improved, so as to achieve controllable product quality, low cost, shorten Effect of preparation time

Inactive Publication Date: 2017-06-13
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method needs to use lye and acid, which will produce acid-base wastewater, which is not friendly to the environment; no enzyme activator is added to the protease, and the enzymatic hydrolysis efficiency needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of antihypertensive peptide derived from the adductor muscle of thick-shelled mussel, the specific operation steps:

[0019] 1) Pretreatment: Take out the adductor muscle of thick-shelled mussels, wash with water for 3 to 5 times, homogenate at high speed, add deionized water according to the ratio of material to liquid volume 1:20~40, soak for 0.5~1.2h, Stir, the stirring rate is 200~400r / min, and filter. The mussel adductor muscle cells were destroyed by high-speed homogenization, allowing the substances in the cells to flow out. Inorganic salts, metal ions and other water-soluble impurities of small molecules are dissolved in deionized water, and grease floats on the water surface, and these impurities are removed by filtration;

[0020] 2) Enzyme hydrolysis: add compound enzyme preparation to the precipitation, the compound enzyme preparation is added at a mass ratio of 1:200~1:400 to the substrate, the composition and weight parts of the compound enzyme pre...

Embodiment 2

[0025] A kind of antihypertensive peptide derived from the adductor muscle of thick-shelled mussel, the most preferred operation steps:

[0026] 1) Pretreatment: Take out the adductor muscle of thick-shelled mussels, wash it with water for 4 times, pulverize it with a pulverizer, and homogenize it at a high speed at a rate of 3000r / min. Add deionized water according to the volume ratio of material to liquid at 1:30, soak for 1.0h, stir at a stirring rate of 280r / min, and filter. The mussel adductor muscle cells were destroyed by high-speed homogenization, allowing the substances in the cells to flow out. Inorganic salts, metal ions and other water-soluble impurities of small molecules are dissolved in deionized water, and oils and fats float on the water surface, and these impurities are removed by filtration;

[0027] 2) Enzyme hydrolysis: add a compound enzyme preparation to the precipitation, the compound enzyme preparation is added at a mass ratio of 1:250 to the substrat...

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Abstract

The invention provides an antihypertensive peptide derived from adductor muscle of mytiloida. The preparation steps are as follows: 1) preprocessing; 2) enzymolysis; 3) ultrafiltration; 4) purifying; and 5) drying. The beneficial effects are as follows: inorganic salt, metal ion and other micro-molecular water-soluble impurities and grease are removed in the manner of soaking with deionized water; the enzymolysis condition is mild and the antihypertensive peptide is not damaged; compound enzyme hydrolysis is adopted, the enzymolysis is sufficient, the acquired molecular weight is low, the antihypertensive peptide is easily absorbed by human bodies and the protein loss is prevented; when a centrifugal ultra-filtration pipe is used for extracting the antihypertensive peptide, no concentration gradient exists, the separation speed is high, the protein loss is avoided, the desalination effect is good, and the extraction rate of the antihypertensive peptide is high; through ultra-filtration again, the other proteins with the molecular weight of more than 30-50KD can be effectively removed, the antihypertensive peptide can be further purified and the acquired antihypertensive peptide is high in purity; the invention has the advantages of simple operation steps, advanced production technique, controllable product quality, low cost and high economic benefit.

Description

technical field [0001] The invention relates to the technical field of polypeptide extraction, in particular to an antihypertensive peptide derived from the adductor muscle of mussel thick shell. Background technique [0002] Hypertension is a disease that seriously harms human health, and it is also the main factor that causes cardiovascular diseases. Its harm is to cause damage to important organs such as the brain, heart, and kidneys. Stroke and coronary heart disease are the most serious complications of hypertension. Data show that the average incidence rate of the world is 10% to 20%, and the number of deaths due to hypertension reaches 12 million every year. At present, there are more than 120 million hypertensive patients in my country. Therefore, the treatment and prevention of hypertension is a very important topic in today's society. [0003] At present, medical research on hypertension mainly focuses on ACE inhibitors (ACEI). Currently, chemically synthesized...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A61P9/12
CPCC12P21/06C07K1/34
Inventor 陈荫
Owner ZHEJIANG OCEAN UNIV
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