Hippocampus polypeptide with antioxidant and anti-fatigue activity and preparation technology thereof

A technology of anti-fatigue activity and preparation technology, which is applied in the field of hippocampal polypeptide and its preparation, can solve the problems of low efficiency, low biological activity of the product, and long time consumption, and achieve high safety, enrich target peptides, and improve biological activity. Effect

Active Publication Date: 2016-10-26
福州众思知识产权服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a hippocampal polypeptide with anti-oxidation and anti-fatigue activities and a preparation method for the deficiencies and existing problems in the existing processing technology, which solves the time-consuming, low-efficiency, and In order to solve the problem of low biological activity of the product, a preparation process for hippocampal polypeptide with high activity is provided

Method used

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  • Hippocampus polypeptide with antioxidant and anti-fatigue activity and preparation technology thereof
  • Hippocampus polypeptide with antioxidant and anti-fatigue activity and preparation technology thereof
  • Hippocampus polypeptide with antioxidant and anti-fatigue activity and preparation technology thereof

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Embodiment 1

[0028] The preparation method of the hippocampal polypeptide having anti-oxidation and anti-fatigue activities specifically includes the following steps:

[0029] 1) Crush: Select dry hippocampus that has not absorbed moisture and deteriorated, and has a light taste and no peculiar smell, cut it into small pieces of 0.5cm, and grind it with a Chinese herbal medicine grinder;

[0030] 2) Electron beam irradiation-ultrasonic treatment: place the hippocampus powder obtained in step 1) in an electron linear accelerator for irradiation treatment (2.5KGy,), and then dissolve the hippocampus powder in In deionized water, stir magnetically for 25 minutes, and finally perform ultrasonic treatment (600W, 50°C, 30min);

[0031] 3) Controllable enzymatic hydrolysis: adjust the pH of the hippocampal solution obtained in step 2) to 6.5, and use 4000U / g 海马 Add an appropriate amount of papain, and enzymatically hydrolyze in a water bath for 40 minutes at 60°C; after cooling to room temperature...

Embodiment 2

[0036] The preparation method of the hippocampal polypeptide having anti-oxidation and anti-fatigue activities specifically includes the following steps:

[0037] 1) Crushing: Select dry hippocampus that has not absorbed moisture and deteriorated, and has a light taste and no peculiar smell, cut it into small pieces of 1 cm, and grind it with a Chinese herbal medicine grinder;

[0038] 2) Electron beam irradiation-ultrasonic treatment: Place the hippocampus powder obtained in step 1) in an electron linear accelerator for irradiation treatment (3.0KGy,), and then dissolve the hippocampus powder in In deionized water, stir magnetically for 20 minutes, and finally perform ultrasonic treatment (400W, 50°C, 25min);

[0039] 3) Controllable enzymatic hydrolysis: adjust the pH of the hippocampal solution obtained in step 2) to 7.0, and use 3000U / g 海马 Add an appropriate amount of papain, and enzymatically hydrolyze in a water bath for 30 minutes at 60°C; after cooling to room tempera...

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Abstract

The invention discloses hippocampus polypeptide with antioxidant and anti-fatigue activity and a preparation technology thereof. Dried hippocampus is used as a raw material and undergoes crushing, electron beam irradiation-ultrasonic treatment, composite enzymatic hydrolysis, ultrafiltration and freeze-drying so as to obtain the hippocampus polypeptide. By combining electron beam irradiation technology and ultrasonic treatment for preparation of the hippocampus polypeptide, enzymolysis velocity of hippocampus can be effectively raised, release of target peptide fragments is accelerated, and antioxidant activity of the enzymolysis product is improved. On the other hand, by the controllable enzymolysis technology and by controlling hydrolysis condition and degree of hydrolysis, target molecular weight distribution of peptide products can be obtained as much as possible. Meanwhile, nutritional value of the enzymolysis product can be greatly preserved under mild enzymolysis conditions, and safety is extremely high. In addition, by filtering the enzymolysis product with filter membrane bags, molecular weight of the hippocampus polypeptide is below 5,000 Da, purity is high, and the product has obvious anti-fatigue efficacy.

Description

technical field [0001] The invention belongs to the technical field of food processing, and specifically relates to a hippocampal polypeptide with anti-oxidation and anti-fatigue activities and a preparation process thereof. Background technique [0002] Hippocampus is a sea dragon family animal, warm in nature and sweet in taste. It is a traditional tonic Chinese medicine, which has the functions of warming kidney and strengthening yang, dispelling stagnation and reducing swelling. The 2005 edition of the Chinese Pharmacopoeia stipulates that the types of seahorses that can be used as medicine include the linear seahorse of the family Sea Dragon ( Hip-pocampus kelloggi Jordan et Snyder ), Thorn seahorse ( Hippo-campus histrix Kaup ), seahorse ( Hippocampus kuda Bleeker ), three-spotted seahorse ( Hippocampus trimaculatus Leach ) and small seahorses (sea maggots) ( Hippocampus japonicus Kaup ) dry body. The medicinal use of hippocampus has a long history, and it h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 郭泽镔林端权贾祥泽王健一郑宝东张怡
Owner 福州众思知识产权服务有限公司
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