Preparation method of stolephorus commersonii immunoloregulation peptide

Abstract

The invention relates to a preparation method of stolephorus commersonii immunoloregulation polypeptide. The preparation method comprises the following steps: selecting a raw material stolephorus commersonii for enzymatic hydrolysis to obtain stolephorus commersonii protein peptide, effecting the obtained stolephorus commersonii protein peptide for in-vitro cultured macrophage for screening enzymatic hydrolysis conditions, and separating and purifying the material to obtain a stolephorus commersonii immunoloregulation peptide product. The method comprises the following steps: a) cleaning a rawmaterial stolephorus commersonii to prepare slurry; b) enzymatic hydrolysis: selecting protease for performing enzymatic hydrolysis on the stolephorus commersonii slurry to prepare enzymatic hydrolysis liquid; c) ultrafiltration: adding the enzymatic hydrolysis liquid in a ultrafiltration system, using ultrafilter membranes with 10KD, 5KD, and 3KD for ultrafiltration, collecting the enzymatic hydrolysis liquid with molecular weights being greater than 10KD, 10-5KD, and 5-3KD and less than 3KD; and d) performing gel column chromatography on the enzymatic hydrolysis liquid by using a glucan gelSephadex G25 and performing buffer solution eluting, respectively collecting peaks, effecting the in-vitro cultured macrophage and screening enzymatic hydrolysis condition to obtain the correspondingpeak with biological activity, and using an anti-phase high performance liquid chromatography for purifying; and e) through purifying with the anti-phase high performance liquid chromatography and amino acid sequence detection, obtaining a stolephorus commersonii immunoloregulation peptide.

Description

technical field [0001] The invention relates to a method for preparing an immunomodulatory peptide of male fish, which belongs to the technical field of immune biological products. Background technique [0002] The immune system is an important system for the body to defend itself. After harmful substances such as pathogens enter the body, the immune system automatically performs immune regulation, and maintains the stability of the internal environment of the human body by identifying, blocking or swallowing antigens. However, abnormal immune regulation can also cause certain damage to the human body, such as immunodeficiency disease, autoimmune disease, hypersensitivity reaction and so on. At present, cancer and tumor diseases caused by low immunity are in a stage of high incidence. However, the continuous reduction of terrestrial resources and the rapid development of marine exploration technology make the application and development of marine biological resources more ...

AI Technical Summary

Problems solved by technology

However, abnormal immune regulation can also cause certain damage to the human body, such as immunodeficiency disease, autoimmune disease, hypersensitivity reaction, etc.