Method for preparing high-content fenugreek gum

A fenugreek gum, high-content technology, applied in the field of separation, can solve the problems of easy carbonization of protein, easy protein corruption, skin allergies, etc.

Inactive Publication Date: 2012-10-24
CHENGDU WAGOTT PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, when these extracts are applied, there is a huge risk. If they are added to cosmetics, due to the high content of plant protein, it will cause skin allergies in some people; if applied to oil extraction, the protein and other ingredients are too high and easy to carbonize; in fenugreek During the preservation of the gel solution, if the protein is too high, it will be easily corrupted
And there is no relevant report on the method of preparing low-protein high-content fenugreek gum from fenugreek

Method used

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  • Method for preparing high-content fenugreek gum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1～9

[0045] Examples 1-9 of the present invention use industrial-grade fenugreek gum powder as raw material, with a polysaccharide content of 55% and a protein content of 6.5%. Use 6-10 times the amount of 70%-90% ethanol as the medium, reflux extraction at 80-90°C for 1-3 hours, remove steroidal saponins, trigonelline, flavonoids and other impurities, and then use 5-15 times the amount of petroleum ether 70 ℃~80℃ Reflux extraction for 2h to remove fatty oil.

[0046] The raw materials after impurity removal are dried, and the organic solvent is volatilized. Take 1 part of the processed raw material and add 5 parts of 50% ethanol, stir well, add purified water to dissolve, and prepare 0.5% fenugreek colloid. Centrifuge to remove the supernatant. The supernatant was heated to 45°C, and 30u / mg of low-level protease was added for enzymatic hydrolysis for 4.5 hours. The liquid after enzymatic hydrolysis is pressurized and passed through 1.2 times the volume of ion resin, and the dra...

Embodiment example 10～18

[0052] Examples 10-19 of the present invention use industrial-grade fenugreek gum powder as raw material, with a polysaccharide content of 55% and a protein content of 6.5%. Use 8 times the amount of 80% ethanol as the medium, reflux extraction at 85°C for 2 hours, remove alcohol-soluble impurities such as steroidal saponins, trigonelline, flavonoids and other impurities, and then reflux extraction with 8 times the amount of petroleum ether at 70°C for 2 hours to remove fatty oil Wait.

[0053] The raw materials after impurity removal are dried, and the organic solvent is evaporated. It is measured that the polysaccharide content in the processed fenugreek gum is 68.5%, and the protein content is 6.7%. The processed raw materials were divided into 9 parts, 5 times the amount of 50% ethanol was added to each part, fully stirred evenly, purified water was added to dissolve, and 0.5% fenugreek colloid was prepared. Centrifuge to remove the supernatant.

[0054] Heat the supern...

Embodiment example 19

[0059] Take 100 g of fenugreek gum powder as the raw material, add 8 times the amount of 85% methanol, and extract twice at 80°C for 2 hours each time. Extract once more with 8 times the amount of petroleum ether at 75°C. filter. drying. Add 2 times the amount of 99% ethanol and stir evenly, then quickly add 10,000ml of purified water, continue to add 20,000ml of purified water after stirring evenly, centrifuge, heat the supernatant to 45°C, add activated protease solution, the amount of protease is 30u / mg substrate amount, after enzymatic hydrolysis for 3 hours, the liquid was passed through 1.6 times ion resin, and the lost liquid obtained was spray-dried.

[0060] The polysaccharide content in the finished product is 95.6%, the protein content is 0.13%, and the viscosity is 5,710mPaS

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Abstract

The invention relates to a method of preparing high-content fenugreek gum from industrial grade fenugreek gum. The method is characterized in that through reflux purification, high speed centrifugation, enzymolysis, resin adsorption and drying, the high-content fenugreek gum which has a pure white color, no taste and no smell, is a transparent solution and has mannogalactan content great than or equal to 95%, protein content less than or equal to 1.0% and viscosity great than or equal to 5600mPaS is obtained.

Description

technical field [0001] The invention belongs to the field of separation methods, in particular to a method for preparing high-purity fenugreek gum from industrial grade fenugreek gum Background technique [0002] Fenugreek gum (fenugreek gum) is a white or light yellow amorphous powder isolated from the endosperm of dry mature seeds (fenugreek seed) of fenugreek (Trigonella foenum-graecum L.). It is galactomannan. The molecular structure is as follows: [0003] [0004] Molecular structure of galactomannan, the main component of fenugreek gum [0005] Decomposition point of galactomannan: 307-311°C, soluble in cold water, hot water, sodium hydroxide, potassium hydroxide solution, insoluble in various organic solvents. Elemental analysis: C 36.66%, H 6.37%, nitrogen-free, the composition conforms to the chemical formula (C 6 h 10 o 5 ).nH 2 O. Its average molecular weight is close to 235,000, which is a neutral mucopolysaccharide. In galactomannan, galactose:manno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12S3/02
Inventor 杨江勇张国芬秦岭
Owner CHENGDU WAGOTT PHARMA
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