Method for preparation of soybean glycoprotein from defatted soybean meal

A technology of glycoprotein and soybean meal, applied in the field of preparation of glycoprotein, can solve the problem of low utilization degree of defatted soybean meal in deep processing

Active Publication Date: 2015-04-15
威海市桢昊生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to various reasons, the degree of deep processing and utilization of defatted soybean mea

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for preparing soybean glycoprotein by utilizing defatted soybean meal, through the following process steps:

[0032] (1) Selection and processing of raw materials

[0033] Select and select the remaining defatted soybean meal pressed by a soybean oil production plant as a raw material for subsequent use;

[0034] Select the compound protease for subsequent use;

[0035] Select commercially available qualified food-grade sodium hydroxide and high-efficiency activated clay for subsequent use;

[0036] Select the compound bean product defoamer produced and sold by Zhengzhou Dazhi Food Co., Ltd., for standby;

[0037] The composite flocculant No. Ⅰ and composite flocculant No. Ⅱ produced by Weifang Lvshui Chemical Co., Ltd. were selected as flocculants in a ratio of 1:1, and they were made into 20% concentration solution, spare;

[0038] (2), soaking, pH adjustment treatment

[0039] Put the defatted soybean meal in step (1) into the enzymatic hydrolysis tank...

Embodiment 2

[0052] A method for preparing soybean glycoprotein by utilizing defatted soybean meal, through the following process steps:

[0053] (1) Selection and processing of raw materials

[0054] Select and select the remaining defatted soybean meal pressed by a soybean oil production plant as a raw material for subsequent use;

[0055] Select papain and alkaline protease for subsequent use;

[0056]Select commercially available qualified food-grade sodium hydroxide and high-efficiency activated clay for subsequent use;

[0057] Select the compound bean product defoamer produced by Zhengzhou Dazhi Food Co., Ltd. for standby;

[0058] The composite flocculant No. Ⅰ and composite flocculant No. Ⅱ produced by Weifang Lvshui Chemical Co., Ltd. were selected as flocculants in a ratio of 1:1, and they were made into 15% concentration solution, spare;

[0059] (2), soaking, pH adjustment treatment

[0060] Put the defatted soybean meal in step (1) into the enzymatic hydrolysis tank, add...

Embodiment 3

[0073] A method for preparing soybean glycoprotein from defatted soybean meal, characterized in that: through the following process steps:

[0074] (1) Selection and processing of raw materials

[0075] Select and select the remaining defatted soybean meal pressed by a soybean oil production plant as a raw material for subsequent use;

[0076] Select flavor protease, papain, alkaline protease, spare;

[0077] Select commercially available qualified food-grade sodium hydroxide and high-efficiency activated clay for subsequent use;

[0078] Select the compound bean product defoamer produced by Zhengzhou Dazhi Food Co., Ltd. for standby;

[0079] Select the composite flocculant No. Ⅰ and composite flocculant No. Ⅱ produced by Weifang Lvshui Chemical Co., Ltd., and use the composite flocculant No. solution, spare;

[0080] (2), soaking, pH adjustment treatment

[0081] Put the defatted soybean meal in step (1) into the enzymatic hydrolysis tank, add 8 times of deionized water...

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PUM

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Abstract

The invention relates to a method for preparation of soybean glycoprotein from defatted soybean meal, the defatted soybean meal as a raw material is soaked with water, after heating and defoaming processing, sodium hydroxide is added for adjustment of pH value to weak alkaline, after cooling, protease is added for enzymatic hydrolysis to obtain an enzymatic hydrolysate; after enzyme inactivation, activated clay and a flocculant are added for static precipitation; supernatant is taken to obtain soybean glycoprotein clear liquid; soybean glycoprotein clear liquid is added with activated carbon for carbon removal clarification and filteration to obtain soybean glycoprotein filtered liquid, the soybean glycoprotein is obtained by concentration and spray drying of the soybean glycoprotein filtered liquid. The enzymatic hydrolysis process has the advantages of mild conditions and easy control of reaction, impurities in the solution can be removed by the flocculation impurity removal process, the clear liquid is decolored and clarified by carbon removal, and the product purity is improved. The method for preparation of soybean glycoprotein from defatted soybean meal has the advantages of advanced technology, reasonable process, and controllable operation, and is suitable for mass production.

Description

technical field [0001] The invention relates to the preparation of glycoprotein from oil cake or other oil-containing seeds, especially a method for preparing soybean glycoprotein from defatted soybean meal. Background technique [0002] my country has a history of eating soybeans for thousands of years. Soybean protein is rich in domestic resources and widely planted. Defatted soybean meal refers to the powder made from soybeans that have been cleaned, tempered, cracked, peeled, pressed, and degreased by the organic solvent n-hexane and similar hydrocarbons under specific conditions, and then roasted and crushed. . The content of soybean protein in defatted soybean meal is as high as 43-48%. Except for the lack of sulfur-containing amino acids, other amino acids have an excellent balance, especially lysine, whose content is as high as 2.5-2.8%. It is the best vegetable protein to replace vegetable protein. one. At present, due to various reasons, the degree of deep proce...

Claims

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Application Information

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IPC IPC(8): A23J1/14
Inventor 倪海平杨众唐茂林唐严芳
Owner 威海市桢昊生物技术有限公司
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