Method for preparing cornu cervi pantotrichum plate enzymatic hydrolysate and cornu cervi pantotrichum plate enzymatic hydrolysate tablet

A technology for antler caps and antlers is applied in the field of biological proteins, which can solve problems to be further deepened, and achieve the effects of extensive social and economic benefits, short enzymatic hydrolysis time and stable results.

Inactive Publication Date: 2017-12-29
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the enzymatic hydrolysis of the active protein of deer horn cap and the anti-inflammatory effect of the hydrolyzate needs to be further deepened

Method used

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  • Method for preparing cornu cervi pantotrichum plate enzymatic hydrolysate and cornu cervi pantotrichum plate enzymatic hydrolysate tablet
  • Method for preparing cornu cervi pantotrichum plate enzymatic hydrolysate and cornu cervi pantotrichum plate enzymatic hydrolysate tablet

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preparation example Construction

[0020] The present invention relates to a kind of preparation method of deer horn cap enzymolyzate, comprising:

[0021] 1). After breaking the antler cap, decocting and extracting with water to obtain an extract, centrifuging the extract to discard the precipitate to obtain a supernatant;

[0022] 2). Concentrate the supernatant, refrigerate and solidify, cut into pieces, fully dry and pulverize to obtain antler cap rubber powder;

[0023] 3). The antler cap rubber powder was dissolved in water, and trypsin was added to enzymatically hydrolyze it to obtain an antler cap enzymatic hydrolyzate.

[0024] The method has a high degree of hydrolysis of deer antler protein, and has excellent anti-inflammatory activity.

[0025] Preferably, in the method for preparing the enzymatic hydrolyzate of antler cap as described above, in step 1), the antler cap is crushed to a particle size of 0.5 cm to 1.5 cm;

[0026] More preferably, it is crushed to a particle size of 0.8 cm to 1.2 cm,...

Embodiment 1

[0051] This embodiment provides a preparation method of antler cap enzymatic hydrolyzate sheet

[0052] The antler caps were broken into 0.5cm pieces, 10 times the amount of water was added, decocted and extracted at 110°C for 3.5 hours, a total of 4 extractions were made, and the extracts were combined.

[0053] Centrifuge extract: 4000r / min, centrifuge for 12min, and combine the supernatant.

[0054] Concentrate until the water content reaches 25%, pour it into a tray covered with silicone oil paper to refrigerate, cut into pieces, dry fully, and crush.

[0055] Take 100g of dried and crushed antler cap glue, add deionized water 2100, adjust the pH to 7.8, dissolve at 36°C, add trypsin after constant temperature, stir for 4.5h, boil in water for 20min at 80°C to inactivate the enzyme, filter, concentrate, and freeze Dry.

[0056] Freeze-dried enzymatic hydrolyzate, crushed, added lactose, magnesium stearate and pressed into tablets.

Embodiment 2

[0058] This embodiment provides a preparation method of antler cap enzymatic hydrolyzate sheet

[0059] The antler caps were broken into 1.5cm pieces, 20 times the amount of water was added, decocted and extracted at 130°C for 2.5 hours, extracted twice in total, and the extracts were combined.

[0060] Centrifuge extract: 5000r / min, centrifuge for 8min, and combine the supernatant.

[0061]Concentrate until the water volume reaches 30%, pour into a tray covered with silicone oil paper to refrigerate, cut into pieces, fully dry, and crush.

[0062] Take 100g of dried and crushed antler cap glue, add 2300g of deionized water, adjust the pH to 8.2, dissolve at 38°C, add trypsin after constant temperature, stir for 3.5h, boil at 90°C for 10min to inactivate the enzyme, filter, concentrate, freeze Dry.

[0063] Freeze-dried enzymatic hydrolyzate, crushed, added lactose, magnesium stearate and pressed into tablets.

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Abstract

The invention relates to the technical field of biological proteins, in particular to an enzymatic hydrolysis method for cornu cervi pantotrichum plates and a cornu cervi pantotrichum plate enzymatic hydrolysate tablet. The enzymatic hydrolysis method includes 1), crushing the cornu cervi pantotrichum plates, then adding the cornu cervi pantotrichum plates into water, decocting the cornu cervi pantotrichum plates, carrying out extraction to obtain extract liquid, centrifuging the extract liquid and discarding precipitates to obtain supernatant; 2), concentrating the supernatant, then carrying out cold storage and freezing, and carrying out sufficient drying and smashing to obtain cornu cervi pantotrichum plate gelatin powder; 3), adding the cornu cervi pantotrichum plate gelatin powder into water, dissolving the cornu cervi pantotrichum plate gelatin powder, adding trypsin into the water and carrying out enzymatic hydrolysis to obtain cornu cervi pantotrichum plate enzymatic hydrolysate. The enzymatic hydrolysis method and the cornu cervi pantotrichum plate enzymatic hydrolysate tablet have the advantages that the enzymatic hydrolysis method is high in cornu cervi pantotrichum protein hydrolysis degree, and the cornu cervi pantotrichum plate enzymatic hydrolysate prepared by the aid of the enzymatic hydrolysis method is excellent in anti-inflammation activity.

Description

technical field [0001] The invention relates to the technical field of biological proteins, in particular to an enzymatic hydrolysis method of antler cap and an enzymatic hydrolyzate sheet of antler cap. Background technique [0002] The antler cap is the antler base that falls off in the spring of the next spring after sawing the velvet of the deer family animal sika deer (Cervus nippon Temminck) or red deer (Cervus elaphus Linnaeus). , and antlers belong to the same thing. The "Shen Nong's Materia Medica", which was written more than 2,000 years ago, is listed as the top grade. It has the effects of warming and tonifying kidney yang, promoting blood circulation and reducing swelling, treating yin symptoms and sores, early breast cancer, strengthening muscles and bones, promoting blood circulation and reducing swelling. There are two types of antler hats: receding horns and cutting horns. Most of the receding horns fall off naturally in spring, and it is better to pick up...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/32A61K9/20
CPCA61K35/32A61K9/2018A61K2236/19
Inventor 孙印石刘畅
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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