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Konjac polypeptide extract, as well as preparation method and application thereof

An extract, konjac technology, applied in the field of medicine and biology, can solve the problems of lowering blood sugar, no konjac flying powder diabetes, unable to control blood sugar, etc., and achieves the effects of less toxic side effects and wide application.

Active Publication Date: 2013-12-11
瀚坤食品(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in reality, the blood sugar target rate of diabetic patients is low
Insulin injection is one of the methods to treat diabetes, but it has been found in clinical treatment that insulin injection itself has the following problems: (1) It can only reduce blood sugar during the injection period, and cannot completely control blood sugar according to the blood sugar change curve; (2) It is prone to hypoglycemia. Blood sugar; (3) The absorption stability of insulin is poor, and the absorption difference reaches 52%; (4) Complications cannot be effectively controlled
At present, there is no report that konjac powder can treat diabetes, and there is no related report that konjac polypeptide extract has the function of treating diabetes

Method used

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  • Konjac polypeptide extract, as well as preparation method and application thereof
  • Konjac polypeptide extract, as well as preparation method and application thereof
  • Konjac polypeptide extract, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 A method for preparing konjac polypeptide extract from konjac powder

[0061] (1) Preparation of konjac protein extract:

[0062]Add konjac powder into pure water, stir and mix to obtain an aqueous solution containing konjac powder; adjust the pH value of the aqueous solution containing konjac powder to 8.5 with NaOH solution, and the extraction time is 100min, then centrifuge to remove insolubles, The konjac protein extract is obtained, which contains konjac fly powder protein;

[0063] (2) The first enzymatic hydrolysis reaction:

[0064] Adjust the pH of the konjac protein extract obtained in (1) to 7.0, and the temperature to 60°C, add the first enzymolysis protease, and stir the enzymolysis at a constant temperature. The enzymolysis time is 2.5 hours, and the first enzymolysis solution is obtained. The first enzymolytic protease is alkaline protease and trypsin, and the weight ratio of the first enzymatic protease to konjac flour protein is 3:50.

[00...

Embodiment 2

[0069] Example 2 A method for preparing konjac polypeptide extract from konjac powder

[0070] (1) Preparation of konjac protein extract:

[0071] Add konjac powder into pure water, stir and mix to obtain an aqueous solution containing konjac powder; adjust the pH value of the aqueous solution containing konjac powder to 9.5 with NaOH solution, and the extraction time is 20min, then centrifuge to remove insoluble matter, The konjac protein extract is obtained, which contains konjac fly powder protein;

[0072] (2) The first enzymatic hydrolysis reaction:

[0073] Adjust the pH of the konjac protein extract obtained in (1) to 8.0 and the temperature to 50°C, add the first enzymolysis protease, and stir the enzymolysis at a constant temperature. The enzymolysis time is 4.5 hours, and the first enzymolysis solution is obtained. The first enzymolytic protease is papain, trypsin and neutral protease, and the weight ratio of the first enzymatic protease to konjac flour protein is ...

Embodiment 3

[0079] Embodiment 3 prepares a kind of method of konjac polypeptide extract from konjac flying powder

[0080] (1) Preparation of konjac protein extract:

[0081] Add konjac powder into pure water, the weight ratio of konjac powder to pure water is 1:50, stir and mix to obtain an aqueous solution containing konjac powder; use NaOH solution to adjust the pH value of the above aqueous solution to 9.0, and the extraction time is 50min , and then centrifuged to remove the insoluble matter to obtain konjac protein extract, which contains konjac fly powder protein;

[0082] (2) The first enzymatic hydrolysis reaction:

[0083] Adjust the pH of the konjac protein extract obtained in (1) to 7.5 and the temperature to 55°C, add the first enzymolysis protease, and stir the enzymolysis at a constant temperature. The enzymolysis time is 3 hours, and the first enzymolysis solution is obtained. The first enzymolysis protease described is alkaline protease and neutral protease, and the wei...

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Abstract

The invention belongs to the field of medical biology, and particularly relates to a method for extracting a konjac polypeptide extract from konjac flying powder, the konjac polypeptide extract obtained by the method and an application of the konjac polypeptide extract. In order to increase the content of konjac polypeptide in the konjac polypeptide extract, the optimized extraction technology provided by the invention adopts a complex enzyme to perform enzymolysis on the protein extraction liquid of the konjac flying powder; in the finally obtained konjac polypeptide extract, the content of konjac polypeptide is as high as 85-97%, and the molecular weight of the konjac polypeptide is 350-3500Da. Moreover, the invention also provides an application of the konjac polypeptide extract in preparing a medicine for treating diabetes and diabetic complications as well as a hypoglycemic medicinal composition containing the konjac polypeptide extract. In a word, the konjac polypeptide extract and the medicinal composition thereof provided by the invention have good prospect in medicine.

Description

technical field [0001] The invention belongs to the field of medical biology, and specifically relates to a method for extracting konjac polypeptide extract from konjac powder, and the konjac polypeptide extract obtained by the method and the application of the konjac polypeptide extract. Background technique [0002] Diabetes mellitus (DM) is a group of syndromes caused by environmental and genetic factors. Its basic pathological features are absolute or relative insufficiency of insulin secretion, or peripheral tissue insensitivity to insulin, mainly causing glucose metabolism disorders. A systemic disease involving disorders of fat and protein metabolism. Many evidence-based medical studies, such as the Clinical Trial of Diabetes Control and Complications (DCCT) and the UK Prospective Diabetes Study (UKPDS), have reached a consensus on the significance of intensified blood sugar control. Reaching the blood sugar target can not only prevent or delay chronic complications o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A61K38/00A61K36/888A61K45/00A61P3/10A61P9/10A61P1/16A61P13/12
Inventor 马杰坤
Owner 瀚坤食品(深圳)有限公司
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