3results about How to "Low lignin content" patented technology

The application discloses a gene for regulating stone cells of pear fruits PbTCP 13 The CDS sequence of the gene is shown as SEQ ID NO. 1, and the protein sequence encoded by the gene is shown as SEQ ID NO. 2. The gene is constructed into an overexpression vector, and the overexpression vector is injected into 'Dangshan Quli' pear fruits, so that the stone cell content and the lignin content of the injection part are significantly lower than those of a part injected with an empty control; the gene is introduced into Arabidopsis thaliana to obtain PbTCP13 transgenic Arabidopsis thaliana, and the secondary cell wall of the vessel cell of the transgenic Arabidopsis thaliana is significantly thinned, and the lignin accumulation in the stem is significantly reduced. The gene provides a new gene resource for fruit quality breeding, and is an important candidate gene for future gene engineering improvement of fruit quality breeding.

This invention provides a gene related to the inhibition of lignin synthesis in alfalfa, named MsMYB17, which can be used in plant breeding. Overexpression of this gene in wild-type alfalfa reduces the lignin content by 30.4%–63.0%, and alfalfa overexpressing this gene can be used as germplasm material for forage breeding. This invention provides a new method for reducing the lignin content in alfalfa and improving forage quality.

The application discloses a Punica granatum L. PgIAA9A gene and a coding protein thereof. The application also discloses application of the Punica granatum L. PgIAA9A gene in regulating growth and development of plant kernels, including negative regulation of plant kernel size and lignin accumulation, and especially negative regulation of inner seed coat size and lignin accumulation of Punica granatum L. The Punica granatum L. PgIAA9A gene can be used for negative regulation of inner seed coat size and lignin accumulation (kernel hardness formation) of Punica granatum L., and further lays a foundation for breeding a new Punica granatum L. variety with small and soft inner seed coat.