11results about How to "Low lignin content" patented technology

The invention discloses application of a transcription factor BnaMYB52 in regulation and control of oil content and lignin content of rape seeds. The amino acid sequence of the transcription factor BnaMYB52 is shown as SEQ ID NO.5, or other amino acid sequences which are derived from the amino acid sequence shown as SEQ ID NO.5 through substitution and / or deletion and / or addition of one or more amino acid residues and have the same protein function. An agrobacterium tumefaciens-mediated genetic transformation method is used for performing overexpression and gene editing on the BnaMYB52 gene respectively, and it is found that the BnaMYB52 gene negatively regulates and controls the oil content of rape seeds and positively regulates and controls the lignin content. The method disclosed by the invention has very important application prospects in high-oil-content breeding of the rape and quality improvement of the rape cake meal.

The invention discloses a method for multi-stage extraction of dietary fibers in bean dregs, and belongs to the technical field of biomass material extraction. The method comprises the following steps: washing bean dregs with water and carrying out enzymolysis treatment to obtain enzymolysis bean dregs; dietary fibers are extracted from the enzymolysis bean dregs through alkaline leaching, leaching liquid obtained through alkaline leaching is settled through alcohol, soluble dietary fibers are obtained, and filter residues obtained through alkaline leaching are insoluble dietary fibers; the preparation method comprises the following steps: sequentially carrying out hydrothermal treatment and DES solvent treatment on insoluble dietary fibers to obtain nano cellulose; carrying out oxidation treatment on the nanocellulose to obtain oxidized nanocellulose; according to the method, soluble dietary fibers in the bean dregs can be extracted, and insoluble dietary fibers can be fully extracted and utilized to obtain polysaccharides and micro-nano cellulose with high additional value, so that agricultural and sideline product wastes are fully utilized, the processing additional value of the agricultural and sideline product wastes is improved, and the pollution to the environment is reduced.

The invention relates to the technical field of biology, in particular to a method for solid state fermentation of bagasse by using white rot fungi, which comprises the following steps: (1) activation and propagation of strains: pouring the white rot fungi into a PDA culture medium for activation and propagation; (2) raw material preparation: air-drying fresh bagasse, adding water to adjust the water content of the bagasse to 60-70%, and adjusting the pH to 4-6 with glacial acetic acid; and (3) preparing the bagasse feed, namely mixing the white rot fungi subjected to expanding culture according to 1-5% of the weight of an air-dried substance with bagasse of which the initial pH is adjusted to be 4-6, adding 1-5% of soluble sugar, and fermenting for 10-45 days to obtain the fermented bagasse feed. According to the white rot fungus fermented bagasse prepared by the method disclosed by the invention, the hemicellulose content of the bagasse can be reduced to 26.62%-22.45%, the lignin content can be reduced to 10.67%-8.66%, the crude protein content of the bagasse can be increased to 1.61%-2.12%, the lactic acid content can be increased to 26.57 mg / L-181.69 mg / L, and high-quality feed application of the bagasse can be realized.

The invention provides a culture medium for reducing the content of broussonetia papyrifera esterase and a tissue culture method, and belongs to the technical field of broussonetia papyrifera tissue culture. The culture medium for reducing the content of the broussonetia papyrifera esterase provided by the invention comprises an induction culture medium, a proliferation culture medium, a differentiation proliferation culture medium and a rooting culture medium, wherein the induction culture medium is composed of an MS culture medium, 6-BA and IAA; the proliferation culture medium is composed of a 1 / 2 MS culture medium, NAA, KT and IBA; the differentiation and proliferation culture medium consists of a B5 culture medium, folic acid, TDZ, 2, 4-D, sucrose and agar; the rooting culture medium consists of an N6 culture medium, gibberellin 3, ethylene, IAA, cane sugar and carrageenan. The culture medium and the tissue culture method provided by the invention can promote more than 98% of adventitious buds to root, ensure that the survival rate is more than 98% after acclimatization and transplantation, not only can effectively reduce the content of lignin in paper mulberry tissue culture seedlings, but also cannot influence the normal growth of the paper mulberry tissue culture seedlings.

The application discloses a gene for regulating stone cells of pear fruits PbTCP 13 The CDS sequence of the gene is shown as SEQ ID NO. 1, and the protein sequence encoded by the gene is shown as SEQ ID NO. 2. The gene is constructed into an overexpression vector, and the overexpression vector is injected into 'Dangshan Quli' pear fruits, so that the stone cell content and the lignin content of the injection part are significantly lower than those of a part injected with an empty control; the gene is introduced into Arabidopsis thaliana to obtain PbTCP13 transgenic Arabidopsis thaliana, and the secondary cell wall of the vessel cell of the transgenic Arabidopsis thaliana is significantly thinned, and the lignin accumulation in the stem is significantly reduced. The gene provides a new gene resource for fruit quality breeding, and is an important candidate gene for future gene engineering improvement of fruit quality breeding.

The application discloses a method for preparing high-yield galacturonic acid by combining chemical and biological methods, which comprises the following steps: firstly, mixing gelidium and a maleic acid solution, and performing microwave heating and acidolysis to obtain D-galactose; and then taking engineered Pseudomonas putida as a biological catalyst to efficiently oxidize the D-galactose into galacturonic acid. In the application, the D-galactose obtained in the microwave heating and acidolysis step has high concentration and high yield, and the D-galactose can be completely oxidized into galacturonic acid in the subsequent biological catalysis step. The method does not need strong acid or special catalyst, is green and environment-friendly, has low raw material cost, simple process, high galacturonic acid yield, and realizes high-value development and utilization of the biomass gelidium.

The invention discloses an application of a zmerf13 gene in regulation and control of corn straw quality, belongs to the technical field of plant genetic engineering, and provides an application of the zmerf13 gene in regulation and control of corn straw glycolysis efficiency, and the nucleotide sequence of the zmerf13 gene is shown as SEQ ID NO.1. The invention also discloses application of the zmerf13 gene in regulation and control of synthesis of vascular bundles of corn straws, regulation and control of cellulose content of the corn straws and regulation and control of lignin content of the corn straws. According to the invention, by planting a mutant with the zmerf13 gene knocked out and comparing the mutant with a wild type corn plant, it is found that the zmerf13 gene knocked out can promote the improvement of corn glycolysis efficiency, so that the quality of corn straws is regulated and controlled, and the quality of the corn straws is improved.

This invention provides a gene related to the inhibition of lignin synthesis in alfalfa, named MsMYB17, which can be used in plant breeding. Overexpression of this gene in wild-type alfalfa reduces the lignin content by 30.4%–63.0%, and alfalfa overexpressing this gene can be used as germplasm material for forage breeding. This invention provides a new method for reducing the lignin content in alfalfa and improving forage quality.

The invention discloses a preparation method of a biomass metal corrosion inhibitor. The preparation method is characterized by comprising the following steps: (1) washing and drying a biomass material; (2) crushing the biomass material; (3) preparing a biomass depolymerization solvent; (4) depolymerizing the biomass powder in a solvent; (5) filtering the depolymerized product at normal temperature and normal pressure to separate liquid from solid; and (6) rectifying the separated liquid to remove the organic acid solvent to obtain the metal corrosion inhibitor. The biomass is wood biomass such as passion fruit shells or straws. According to the method, organic acid reacts with hydrogen peroxide to generate peroxy acid, the peroxy acid oxidizes beta-O-4 bonds in the passion fruit shell or straw lignin, so that the lignin is depolymerized into lignin fragments with low molecular weight, the content of the lignin obtained by the method is high, and a good slow release effect can be achieved without additionally adding a chemical corrosion inhibitor. Meanwhile, the depolymerization residues are passion fruit shell or straw cellulose and can be directly sold, and high-added-value utilization of passion fruit shells or straw is achieved.

The application discloses a Punica granatum L. PgIAA9A gene and a coding protein thereof. The application also discloses application of the Punica granatum L. PgIAA9A gene in regulating growth and development of plant kernels, including negative regulation of plant kernel size and lignin accumulation, and especially negative regulation of inner seed coat size and lignin accumulation of Punica granatum L. The Punica granatum L. PgIAA9A gene can be used for negative regulation of inner seed coat size and lignin accumulation (kernel hardness formation) of Punica granatum L., and further lays a foundation for breeding a new Punica granatum L. variety with small and soft inner seed coat.

The invention provides salix matsudana 1-aminocyclopropane-1-carboxylate oxidase capable of regulating and controlling the fast-growing property of plants as well as a coding gene and application thereof, and relates to the technical field of gene engineering. The gene is derived from salix matsudana and is named as SmACO17 gene, the sequence of the gene is as shown in SEQ ID NO: 1, and the protein coded by the gene is 1-aminocyclopropane-1-carboxylate oxidase. The gene and the protein coded by the gene provided by the invention have the function of 1-aminocyclopropane-1-carboxylate oxidase, the obtained transgenic arabidopsis thaliana containing the SmACO17 gene causes the arabidopsis thaliana to generate changes such as plant height shortening, growth and development slowing and the like, and the ACO17 gene of the salix matsudana is silenced by utilizing an artificially synthesized Silence-ACO17 sequence. After the expression of SmACO17 is inhibited, the plant is higher, the leaf is widened, and the plant has better fast-growing property.