Bleaching compound enzyme for papermaking and preparation method of bleaching compound enzyme

A compound enzyme and laccase technology, applied in pulp bleaching, etc., can solve the problems of increased possibility of pulp yellowing, low laccase output, poor stability, etc.

Active Publication Date: 2015-04-08
黄冈晨鸣浆纸有限公司
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AI Technical Summary

Problems solved by technology

Ligninase can directly act on lignin to degrade lignin, mainly lignin peroxidase, manganese peroxidase, laccase and cellobiose dehydrogenase secreted by white rot fungi. Research hotspots mainly focus on the biological bleaching of laccase, a multi-copper oxidase, also known as polyphenol oxidase, which mainly oxidizes phenolic lignin structural units, but only less than 20% of natural wood lignin In addition, fungal laccase has a large molecular weight (about 70,000 Da) and it is difficult to penetrate into wood to act on lignin molecules. To fully exert its enzyme activity, a laccase medium must be added, usually violuric acid (VA) and 1-hydroxybenzotriazole (HBT), depending on different bleaching processes, and the yield of laccase is small and the stability is poor
[0005] Chinese patent CN 102978986 A discloses a method for preparing pulp by biological enzyme method. The mass percentage content of biological enzyme components in the biological enzyme preparation liquid is: 20% cellulase, 10% hemicellulase, lignin degradation 10% enzyme, 20% amylase, 5% lipase, 20% pectinase and laccase; the cellulase in it will degrade the active ingredient cellulose in pulp, which is difficult to control and seriously affects the physical and mechanical properties of paper. At the same time, the above-mentioned biological enzymes are suitable for the pulping process, not the bleaching process, and the single use of laccase is difficult to exert its enzyme activity, and it cannot act on lignin well, and the possibility of pulp yellowing increases
Chinese patent CN 103555701 A discloses a production method of a compound enzyme solution for pulp bleaching, which is characterized in that the enzymes of each component are weighed, mixed and prepared in a compound tank, preserved, inspected, packaged, and then stored in a warehouse at 5°C. The enzymes of each component are alkaline pectinase, xylanase, mannanase and ligninase; the enzyme types and characteristics of the above-mentioned composite enzyme solution are single, the activity of ligninase is low, and the specific details of ligninase are not disclosed. species, industrialization is difficult to achieve, and lignin cannot be completely eliminated fundamentally, the bleaching effect is still unsatisfactory, and it is not easy to preserve, the storage stability is poor, and the commercial application is limited

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  • Bleaching compound enzyme for papermaking and preparation method of bleaching compound enzyme
  • Bleaching compound enzyme for papermaking and preparation method of bleaching compound enzyme

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1 raw material preparation

[0075] 1. The preparation of alkaline xylanase: comprise the following steps

[0076] (1) Activation of bacteria

[0077] Inoculate the slant strain of the well-preserved thermophilic Bacillus CCTCC M 2013537 on the slant medium, culture at 45°C for 36 hours to activate the strain, and activate it 3 times;

[0078] The slant medium consists of: beef extract 10g, sodium chloride 12g, peptone 20g, glucose 5g, agar 20g, Chinese herbal medicine powder 10g, distilled water 1000mL, pH value 10, sterilized at 121°C for 20 minutes;

[0079] The preparation method of described Chinese herbal medicine powder is as follows:

[0080] In terms of parts by weight, weigh 25 parts of Astragalus membranaceus, 15 parts of Codonopsis pilosula, 12 parts of Bupleurum baicalensis, 12 parts of scutellaria baicalensis, 10 parts of Houttuynia cordata, and 9 parts of angelica sinensis; respectively crush the above-mentioned Chinese herbal medicines until...

Embodiment 2

[0105] Embodiment 2 Determination of high temperature resistant alkaline xylanase enzyme activity (3,5-dinitrosalicylic acid colorimetric method, referred to as DNS method)

[0106] 1. Reagents and solutions

[0107]1.1 Except for special requirements, the water used is third-grade water in accordance with the provisions of GB / 6682-1992, and the chemicals are all analytically pure unless otherwise specified.

[0108] 1.2 DNS Reagent

[0109] Weigh 10g of 3,5-dinitrosalicylic acid and add it into 500ml of water, add 16g of sodium hydroxide in several times, stir to dissolve (temperature <45°C), then add 300g of potassium sodium tartrate in several times, stir until completely dissolved, After cooling, dilute to 1000ml with water. Store the brown bottle in a dark place at room temperature and use it after one week (if there is precipitation, use it after filtration).

[0110] 1.3 0.1mol / L, pH5.0 acetic acid-sodium acetate buffer

[0111] Take 1.8ml of glacial acetic acid, ad...

Embodiment 3

[0139] The thermostability analysis of embodiment 3 alkaline xylanase

[0140] The thermal stability of the enzyme was analyzed, and the crude enzyme liquid prepared by the alkaline xylanase in Example 1 was placed at 40°C, 45°C, 50°C, 55°C, 60°C, 65°C, and 70°C respectively, Samples were taken every 10 minutes to determine the enzyme activity. At 40°C, 45°C, 50°C, and 55°C in the crude enzyme solution, the enzyme activity did not decrease for 60 minutes. At 60°C and 65°C, the original enzyme activity is reduced to 95% in 30 minutes, and to 85% in 60 minutes. At 70°C, it will drop to 85% of the original enzyme activity in 30 minutes and 80% in 60 minutes. At 75°C, the enzyme activity is reduced to 80% of the original enzyme activity in 30 minutes, and to 70% in 60 minutes. Compared with the prior art, the same enzyme activity is achieved, and the temperature tolerance is higher.

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Abstract

The invention discloses a bleaching compound enzyme for papermaking and a preparation method of the bleaching compound enzyme. The bleaching compound enzyme for papermaking, which has the advantages of complete enzyme system, good bleaching effect, easiness in preservation and good storage stability, is prepared by taking high temperature-resistant alkaline xylanase as main raw material, scientifically compounding a thermophilic bacillus culture, laccase and a medium thereof, dextranase, EDTA (ethylenediaminetetraacetic acid), a protecting agent, an activator, mannose, lipase, tannase, pectase, a whiteness stabilizer, a nonionic surfactant, an antioxidant and the like, completely retaining paper pulp cellulose and simultaneously dissolving out and degrading lignin to the greatest extent. The bleaching compound enzyme disclosed by the invention can significantly improve yield and quality of paper pulp against broad-leaf wood, coniferous wood and reed yellow slurry, and the whiteness is improved by 10.94%, 11.97% and 11.10% respectively; the yellow index is reduced by 39.84%, 37.31% and 24.32% respectively; the amount of conventional chemical products can be saved by 50-60%; and the purposes of reducing cost and protecting an environment are finally achieved.

Description

technical field [0001] The invention relates to a composite enzyme for papermaking, in particular to a bleaching composite enzyme for papermaking and a preparation method thereof. Background technique [0002] In industrial production, the pulping process is to remove lignin by high-temperature cooking under alkaline conditions. In order to make the purity of dissolved pulping cellulose reach 98%, this requires a large amount of sodium hydroxide to treat the pulp, resulting in Due to serious environmental pollution, many scholars have turned to biological treatment for pulping, and the xylanase used for pulping and bleaching should be heat-resistant and alkali-resistant. Feng Jianliang et al. (Kimura et al.2000) conducted a comparative experiment with xylanase pretreatment of wheat straw and conventional chemical pulping. Xylanase pretreatment improved the degree of delignification of raw materials and could also reduce the jam of pulping. primary value. Xylanase has been ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D21C9/10
CPCD21C9/10
Inventor 邵素英
Owner 黄冈晨鸣浆纸有限公司
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