8 results about "Alkaline xylanase" patented technology

The present application belongs to the technical field of processing eucommia ulmoides, and particularly relates to a method for resolving and pulverizing residual hard-to-degrade plant tissues on eucommia ulmoides glue by using biological enzymes. The present application uses eucommia ulmoides shell crude glue as raw material, and resolves the raw material by using three biological enzymes in the following order: acid protease, alkaline xylanase and acid xylanase. Then, trace colored tissue debris and plant pigments attached to the G glue filament are removed by ultrasonic cleaning with a surfactant to obtain original flocculent eucommia ulmoides pure white fine glue. The acid protease enzymolysis conditions are as follows: pH 3.0, 55 DEG C oscillation for 48 h. The alkaline xylanase enzymolysis conditions are as follows: pH 7.2, 50 DEG C oscillation for 48 h. The acid xylanase enzymolysis conditions are as follows: pH 4.8, 50 DEG C oscillation for 72 h. The present application successfully separates and pulverizes the brown hard shell in the inner layer of eucommia ulmoides from the eucommia ulmoides glue filament, removes all non-glue plant tissues such as the outer epidermis and the hard shell in the inner layer of eucommia ulmoides shell, and obtains high-purity eucommia ulmoides glue after ultrasonic cleaning with a surfactant, with a purity as high as 98.94%.

The present application relates to the field of genetic engineering and protein modification technology, and particularly relates to a novel basic xylanase mutant. The present application is based on wild-type xylanase H1, and provides a mutant containing at least one of the following mutation sites: Q24P, S38V / K / W / H / T / I, G40M / R / K / F / H, D41P, N56L / I / P / K, A57E / D / Y, A59K / R / I / M / H, H61F / Y, A75S, T80M, T103I, T107E, T114Y, D129L, Q132R / M, D135M, N143D, K144R, T149L, Q151N / Y, C154N, D157E, A160E, N165D, V166I, N167S / W, T177V, D192E. The tolerance of the mutant to high temperature and alkaline environment is significantly improved, which is beneficial to its wide application in the field of papermaking.

The present application relates to the technical field of genetic engineering and protein engineering, and particularly relates to a basic xylanase mutant and application thereof. The present application provides a mutant containing a N165H mutation site based on wild-type xylanase H1. Compared with the wild type, the heat resistance of the xylanase mutant is significantly improved, which is conducive to the wide promotion and application of the xylanase mutant in the industrial field.

The present application relates to the field of genetic engineering and protein engineering, and particularly relates to a high specific activity alkaline xylanase mutant. The present application provides a mutant containing H61L mutation site based on wild type xylanase H1. The specific activity of the mutant is significantly higher than that of the wild type, which is beneficial to reduce the production cost and promote its wide application in the industrial field.

The present application relates to the field of genetic engineering and protein modification technology, and particularly relates to a high specific activity basic xylanase mutant and application thereof. The present application takes wild type xylanase H1 as a basis, and provides a mutant containing at least one of the following mutation sites: I37V, A59S, D63E, D104Y, T107M / K, N167G and D192E. Compared with the wild type xylanase H1, the specific activity of the xylanase mutant provided by the present application is generally increased by 8.5%-32.8%; wherein the specific activity of the xylanase mutant containing the D192E single point mutation is the highest, reaching 1625.11 U / mg, which is conducive to reducing the production cost of the enzyme and promoting its wide application in the industrial field.

The invention discloses a high-enzyme-activity alkaline xylanase mutant and a preparation method thereof, and belongs to the field of gene engineering and enzyme engineering. According to the invention, heat-resistant and alkali-resistant xylanase derived from Bacillus halodurans is modified by a semi-rational design method, so that a series of combined mutants M1-M3 with high enzyme activity are obtained. The enzyme activity of the combined mutants M1-M3 is more than 3.5 times that of a wild type, and the enzyme activity of the optimal mutant M1 is 432.6% that of the wild type under the conditions of 70 DEG C and pH 9.0. The combined mutant provided by the invention can promote application in the fields of biomass resource utilization, food or papermaking industry, effectively solves the core problem of low enzyme activity in current xylanase application, and is more beneficial to application in industrial scenes.

The present application provides a termite fungus and the produced alkaline xylanase and application. The screened termite fungus Isoptericola sp. WL6 belongs to alkaliphilic extremophiles, can be cultured under high alkaline conditions (pH 9.0), has the characteristics of effectively inhibiting the pollution of miscellaneous bacteria and being conducive to large-scale fermentation production. The alkaline xylanase protein produced by the termite fungus Isoptericola sp. WL6 has a molecular weight of about 43 kD, and the enzymatic property research shows that the optimal reaction temperature is 50 DEG C, the optimal reaction pH is about 8, especially between pH 6.5-10.5, the relative enzyme activity of the enzyme is all above 80%, which shows that the enzyme has good pH stability, strong stress resistance characteristics of both acid and alkali, meets the basic requirements of industrial enzymes such as papermaking and washing, and has good industrial application prospect.

The present application relates to the technical field of traditional Chinese medicine wastewater treatment, and specifically discloses a method for rapidly treating traditional Chinese medicine wastewater by using activated carbon immobilized enzyme, which comprises the following steps: activated carbon selection, activated carbon pretreatment, immobilized alkaline xylanase, activated carbon with immobilized enzyme treating traditional Chinese medicine wastewater, and filtering the water treated by activated carbon with immobilized enzyme; ordinary medium-speed filter paper is used for preliminary filtration to remove larger impurities and particles; then, a filter membrane with a pore size of 0.22-0.45 um is used for further filtration to remove smaller particles and impurities; and finally, the treated water is obtained. The present application can efficiently degrade natural organic polysaccharide pollutants in traditional Chinese medicine wastewater by using activated carbon immobilized enzyme, improve the biodegradability of wastewater, reduce membrane blockage, reduce operation cost, efficiently reduce the colority, COD, total nitrogen and ammonia nitrogen of the treated water, and thus simplify the traditional Chinese medicine wastewater treatment process and reduce the operation cost of a wastewater treatment plant.