Method for extracting agar from gracilaria with enzymatic method
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A technology for enzymatic extraction of Gracilaria, applied in the field of enzymatic extraction of Gracilaria agar, can solve the problems of residual chloride ions, repeated freezing and thawing, etc., and achieve the effects of simple operation, shortened production cycle and cost, and reduced production cost
Active Publication Date: 2014-03-26
青岛福创环境科技有限公司
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Problems solved by technology
The invention overcomes the problems of strong alkali, high temperature, residual chloride ions, repeated freezing and thawing and other problems in the traditional process, makes the operation simpler and milder, and is suitable for large-scale industrial production
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Embodiment 1
[0014] Weigh 100 g of Gracilaria, cut it into pieces, grind it into powder with a universal grinder, add 1000 mL of water, and soak at 45 °C for 2 h. Adjust the pH value of the soaked Gracilaria solution to 5, add 80 mg cellulase, and react for 6 h; then adjust the pH value to 8-10, add 25 mg compound protease, and react for 2 h.
[0015] After enzymatic hydrolysis, centrifuge to obtain Gracilaria residue, add 800 mL of water to the residue of Gracilaria, adjust pH to 10 with 0.5 mol / L sodium hydroxide solution, add 2 mL of 27.5% hydrogen peroxide, and bleach for 5 min; Finally, add 1M hydrochloric acid solution to make the HCl concentration of the system 0.8%. During this process, stir continuously for 3 minutes to remove most of the soluble impurities and pigments; immediately wash with water until neutral after the acidification treatment, and set aside.
[0016] Add 300 mL of water to the bleached and decontaminated Gracilaria slag, boil in a water bath at 95°C for 1 hour,...
Embodiment 2
[0018] Weigh 1 kg of Gracilaria, cut it into pieces, grind it into powder with a universal grinder, add 8 L of water, and soak at 50°C for 1.5 h. Adjust the pH value of the soaked Gracilaria solution to 4, add 1.0 g cellulase, and react for 8 h; then adjust the pH value to 8.5, add 0.3 g complex protease, and react for 2 h.
[0019] After enzymatic hydrolysis, centrifuge to obtain Gracilaria slag, add 6 L of water to the Gracilaria slag, adjust the pH to 10 with 0.5 mol / L sodium hydroxide solution, add 18 mL of 27.5% hydrogen peroxide, and bleach for 4 min; Finally, add 1M hydrochloric acid solution to make the HCl concentration of the system 0.8%. During this process, stir continuously for 3 minutes to remove most of the soluble impurities and pigments; immediately wash with water until neutral after the acidification treatment, and set aside.
[0020] Add 2 L of water to the residue of Gracilaria after bleaching and removing impurities, boil it in a water bath at 95°C for 1 ...
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Abstract
The invention discloses a method for extracting agar from gracilaria with an enzymatic method. After gracilaria is soaked, cellulose and protease are added for enzymolysis, and gracilaria slag in enzymatic hydrolysate is filtered; after the gracilaria slag is subjected to bleaching, acidification and washing treatment, an appropriate amount of water is added, water is boiled to 95 DEG C for glue extraction, and enzyme activity is killed; a glue solution is filtered while hot, 95% ethyl alcohol with a volume of two to three times of that of the glue solution is added to the glue solution rapidly, precipitation is centrifuged or filtered, dehydration is performed twice with absolute ethyl alcohol, and drying is performed, so that an agar product is obtained. The main ingredient of agar in gracilaria is separated with a clean and efficient biological enzyme treatment technology, and the defects of a conventional alkali treatment technology are overcome; before glue extraction is performed on gracilaria, sodium hypochlorite is replaced with hydrogen peroxide for bleaching treatment, the bleaching effect is remarkable, and the problems of chloride ion residues and pollution caused by a sodium hypochlorite bleaching method are solved; and finally, agar is rapidly precipitated from the glue solution with a simple, convenient and feasible alcohol precipitation method, the production period is greatly shortened, and the cost is greatly reduced.
Description
technical field [0001] The invention belongs to the field of deep processing and comprehensive utilization of marine resources, and relates to a method for extracting agar from Gracilaria by enzymatic method. Background technique [0002] Jiangli is currently one of the main raw materials for agar production at home and abroad. In the past few decades, alkali treatment has become an indispensable process in the process of producing agar in Jiangli. Summary There are three main types of domestic and foreign treatment methods for river fence alkali, namely, low-temperature concentrated alkali method, medium-temperature concentrated alkali method, and high-temperature dilute alkali method. These three methods inevitably use NaOH, which undoubtedly brings certain pollution to the environment. At the same time, along with the rise of the price of alkali and the shortage of energy, the high cost of extracting agar by alkali method also makes the production enterprises fall into a ...
Claims
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Application Information
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