44 results about "Gracilaria conferta" patented technology

The invention discloses a method for extracting agar, fucoidin and protein from gracilaria as a raw material by using an enzyme process. The method comprises the steps of: performing enzymolysis on the gracilaria by using a biological enzymolysis technology, separating protein from enzymatic hydrolysate by using an isoelectric precipitation technology after being concentrated, precipitating the solution after the protein is extracted through alcohol to separate the fucoidin, and performing procedures such as bleaching, glue boiling, freezing, thawing and dewatering on the enzymolysis residue to obtain a high-purity agar product. According to the method, the defect of a process of extracting the fucoidin by using a conventional alkaline process glue extracting and water extraction method is broken, the extraction separation of the agar, the fucoidin and the protein is realized in one step by adopting a biological enzymolysis method, and water saving, energy saving and emission reducing are greatly realized when the comprehensive utilization value of the gracilaria as the raw material is increased. The method accords with relevant national policies.

The invention discloses an ultrasound-assisted preparation method of high strength Gracilaria agar. The method includes: taking Gracilaria as the raw material, conducting intermediate-temperature low-alkaline treatment under intermittent assistance of ultrasound, ultrasound-assisted acidification, ultrasound-assisted bleaching and heavy metal removal, carrying out gel boiling, filtering out impurities, performing cooling, extruding gel, conducting dehydration, drying, and smashing, and then passing the product through a 80-mesh sieve, thus obtaining the high strength Gracilaria agar. The invention adopts intermediate-temperature dilute alkaline treatment on Gracilaria, with NaOH assisted by ultrasound intermittently, so that the alkaline treatment time is shortened, and simultaneously gel loss can be substantially reduced. Ultrasound is employed to assist acidification of a mixed solution of HCl, H2SO4, and C2H2O4, so that the acid consumption is substantially reduced, and the action time is short. Ultrasound is adopted to assist a mixed solution of NaClO, H2O2, and EDTA-Na2 to realize efficient bleaching, and at the same time, heavy metals are removed from the raw material, so that product color and hygienic quality are guaranteed. The method provided in the invention makes use of ultrasound to assist preparation of Gracilaria agar having high strength, good color, and excellent security, and is of great significance in enhancing the technical level of agar preparation and improving product comprehensive quality.

The invention discloses a method for extracting agar from gracilaria with an enzymatic method. After gracilaria is soaked, cellulose and protease are added for enzymolysis, and gracilaria slag in enzymatic hydrolysate is filtered; after the gracilaria slag is subjected to bleaching, acidification and washing treatment, an appropriate amount of water is added, water is boiled to 95 DEG C for glue extraction, and enzyme activity is killed; a glue solution is filtered while hot, 95% ethyl alcohol with a volume of two to three times of that of the glue solution is added to the glue solution rapidly, precipitation is centrifuged or filtered, dehydration is performed twice with absolute ethyl alcohol, and drying is performed, so that an agar product is obtained. The main ingredient of agar in gracilaria is separated with a clean and efficient biological enzyme treatment technology, and the defects of a conventional alkali treatment technology are overcome; before glue extraction is performed on gracilaria, sodium hypochlorite is replaced with hydrogen peroxide for bleaching treatment, the bleaching effect is remarkable, and the problems of chloride ion residues and pollution caused by a sodium hypochlorite bleaching method are solved; and finally, agar is rapidly precipitated from the glue solution with a simple, convenient and feasible alcohol precipitation method, the production period is greatly shortened, and the cost is greatly reduced.

The invention relates to a gracilaria agar production method based on a mathematical model. The gracilaria is subjected to alkali treatment; washing, acidifying and bleaching are carried out on the gracilaria subjected to alkali treatment; gel boiling is carried out on the gracilaria after washing, acidifying and bleaching to obtain gracilaria agar; the gel strength, transparency, 3, 6-anhydro content, sulfate radical content and gel yield of gracilaria agar are measured, the alkali consumption is calculated, a linear equation of experimental data is made, and the linear equation is convertedinto a matrix equation to construct a gracilaria agar quality influence factor kinetic model. According to the method, the alkali consumption and quality of the gracilaria agar under different alkalitreatment processes are calculated by establishing the model, and the method has important significance for the alkali treatment process required for producing the multi-index gracilaria agar and reducing the alkali consumption of the gracilaria agar.

The invention discloses a method for ultrasonic acid extraction of sulfated polysaccharide from gracilaria tenuistipitata, which comprises the steps of: removing impurities of dry gracilaria tenuistipitata by blanching, air-drying, crushing, and carrying out degreasing treatment by using ethanol; using HCI solution for leaching; centrifugating to obtain a filtrate through ultrasonic extraction; adding NaOH into the filtrate for neutralization; concentrating a supernatant fluid after the centrifugation; adding the ethanol into the concentrated supernatant fluid; standing the obtained solution; washing a precipitate by using the ethanol after the centrifugal precipitation; obtaining a crude sulfated polysaccharide product A by decompression drying; preparing the crude polysaccharide A into aqueous solution; adding the ethanol into the aqueous solution; precipitating to obtain algin B; centrifugating the algin B; adding the ethanol into a supernatant fluid; centrifugating and precipitating the supernatant fluid; using the ethanol and acetone for immersion cleaning; and drying to obtain sulfated polysaccharide C. The method combines the advantages of hot water, ultrasonic wave and acid methods and adopts an ultrasonic wave-assisted thermal acid method to extract the sulfated polysaccharide from the gracilaria tenuistipitata, has larger extraction amount of the sulfated polysaccharide, has the advantages of simple and practical process, low needed investment during the production and quick response, and has the technology which is suitable to be widely popularized.

The invention relates to a method for improving gracilaria agar gel strength and belongs to the technical field of food production. The method aims to prepare high-strength agar by adding a microorganism enzyme namely transglutaminase into agar and specifically includes: dissolving agar powder in hot water to prepare 1.5% agar solution, boiling agar for a certain time until the agar is completely dissolved, adding transglutaminases of different concentrations within a certain temperature and pH range, stirring for reaction for a few minutes, standing at the room temperature to enable the surface to be solidified, placing at constant temperature for gelling for a few hours, and measuring the gel strength. The gel strength of the agar is evidently improved when addition amount of the transglutaminases is 0.01%-1.0% (g / g, relative to dry weight of agar powder). The method has the advantages that the agar is assuredly edible by using the biological method for improving gel strength, nontoxic, harmless and pollution-free, and the method is simple in operation and low in production cost.

The invention discloses an agar compound colloid being dissoluble at low temperature and high in gelling strength and a preparation method thereof, and belongs to the technical field of food processing. The agar compound colloid is obtained through the steps that compound agar and auxiliary materials are extruded by a twin-screw extruder, dried and crushed, wherein the compound agar comprises Indonesia agar and gracilaria asparagus agar, the mass ratio of the Indonesia agar to the gracilaria asparagus agar is 1:2, and one or more of corn starch, microcrystalline cellulose, beta-cyclodextrin and carrageenan are adopted as the auxiliary materials. Meanwhile, the invention provides the preparation method of the agar compound colloid. The agar compound colloid has the advantages of being low in dissolving temperature and high in gelling strength; the agar compound colloid can be dissolved at 50 DEG C, and the gelling strength of the dissolved agar at 50 DEG C can reach 625 g.

The invention relates to a preparation method of temperature-sensitive type gracilaria agar complex gel, and belongs to the technical field of gel preparation. The method includes the steps of preparing gracilaria agar from fresh gracilaria serving as the raw materials together with thrice sterile water under specific conditions, mixing gracilaria agar with ethyl alcohol and other substances to form a gracilaria agar ethanol solution, making the solution mixed with chloroacetic acid and other substances under specific conditions, and conducting filtering, concentrating and drying to obtain the gel. The method has the advantages that the medicine carrying capacity is strong, the slow-release time is long, and high stability is achieved; the medicine utilization rate is high and is improved by 10-15%, the preparation steps are simple, and cost is low.

The invention provides a method for extracting agar from gracilaria. The method comprises the following steps: 1) performing alkali treatment: placing and treating gracilaria plants free of impuritiesin an alkali solution, and removing the alkali solution after the treatment is finished; 2) crushing: crushing the gracilaria prepared in step 1) to obtain a crushed feed liquid; 3) heating for extracting agar: supplementing water to the feed liquid obtained in step 2), and performing heating for extracting agar; 4) carrying out deslagging treatment through plate-frame filtration; 5) carrying outdecolorizing treatment by adopting activated carbon; and 6) performing dehydrating and drying to obtain the agar. The gracilaria subjected to alkali treatment is mechanically crushed to form a colloidal solution, the colloidal solution subjected to heating, agar extraction, squeezing and filtration is subjected to decolorizing by using powdered activated carbon, and then undergoes freezing dehydration or squeezing dehydration and drying to obtain the agar product, so the product has a stable yield, a stable quality and a slightly yellow or white appearance. Hydrochloric acid, oxalic acid andthe like are not needed in the whole processing and production process, a chemical bleaching agent is not needed, the water consumption is only about 40% of that of a traditional method, and the generated wastewater amount is also greatly reduced.

The invention relates to the field of genetic engineering technology, and particularly relates to a gracilaria chouae uridine diphosphate (UDP)-glucose pyrophosphorylase (UGPase) gene. The nucleotide sequence of the gene and the amino acid sequence of the coding protein are SEQ ID No.1 and SEQ ID No.2 respectively. According to the invention, the gene sequence is cloned through the gene cloning technology, and a prokaryotic expression vector is established; and enzyme activity detection on recombinant protein proves that the gene has the function of catalyzing UDP-glucose and pyrophosphoric acid to form glucose-1-phosphoric acid and UTP and belongs to a key enzyme coding gene of the synthesis path of agar-agar, starch, cellulose, trehalose, sucrose and the like. The gene has an important application value in increasing the content of economic components including algae agar-agar, starch, cellulose, trehalose, sucrose and the like.

The invention discloses a natural macromolecular water absorbing material and a preparation method thereof. Natural macromolecular gracilaria polysaccharides and sodium alginate are used as main raw materials; the natural macromolecular water absorbing material has great safety to people, particularly infants; the water absorbing multiples reach tens to hundreds of multiples of the self weight and the tensile strength is great; the natural macromolecular water absorbing material has a good water-retaining performance and the product can be naturally degraded.

The invention discloses agaro-oligosaccharide iron and a preparation method thereof. The agaro-oligosaccharide iron is prepared by using Gracilaria agar as a raw material through steps of refining, acid degradation, high-pressure pulse electric field-assisted chelation, membrane separation, freeze-drying and pulverization. The refining step increases the neutral sugar content significantly, and after acid degradation, the chelation of agaro-oligosaccharide and iron ions can be promoted under the catalysis of a high-voltage pulsed electric field. The method has advanced and reasonable technology and high production efficiency; the prepared agaro-oligosaccharide iron product has high iron content, has good oxidation resistance to enhance cell viability, and promotes the iron absorption by body, and has high bioavailability.

The invention relates to the technical field of film materials, and provides a degradable film, which is prepared from the following raw materials in parts by weight: 0.5 to 2 parts of Gracilaria chouae polysaccharide, 0.2 to 1 part of chitosan, 0.05 to 0.1 part of sodium carboxymethyl cellulose, 0.5 to 3 parts of a low-temperature co-melting solvent and 94 to 99 parts of distilled water. The preparation method comprises the following steps: by taking the Gracilaria chouae polysaccharide, chitosan and sodium carboxymethyl cellulose as raw materials, the low-temperature co-melting solvent as a dissolution promoter and a plasticizer and the distilled water as a solvent, heating and stirring until a colorless transparent liquid is formed, pouring the colorless transparent liquid into a container, drying, and uncovering the film, so as to obtain the degradable film. The degradable film prepared by the method is wide in raw material source, low in cost, simple to operate and degradable, so that the degradable polysaccharide film has a wider application prospect, and the application field and research direction of the gracilaria brittleness polysaccharide and the low-temperature eutectic solvent are widened.

The invention discloses a production method of instant gracilaria convenience food. The production method comprises processing steps as follows: gracilaria is placed in boiling water to be blanched for 1-2 min for fixation and enzyme deactivation, then after cooled by ice water, the gracilaria is placed in a flavoring liquid containing a color fixative and a protein cross-linking agent to be soaked for 3-12 h and is subjected to centrifugal dewatering, freeze thawing and secondary centrifugal dewatering, and moisture content of gracilaria is reduced to 70%-75%; and the obtained gracilaria is added with flavoring oil containing an antioxidant to be uniformly stirred and mixed, a weight ratio of the gracilaria to the flavoring oil is 1: (20-50), the mixture is subjected to vacuum packaging, sterilized at the temperature of 80-85 DEG C for 30-60s and cooled by ice water rapidly, and accordingly, the instant gracilaria convenience food is prepared. The processing technology is simple, and a product can be sterilized through pasteurization and can be stored at the normal temperature; and besides, the obtained instant gracilaria convenience food is free of remarkable fishy and astringent smell of algae, has bright green appearance and crisp and tender taste and can be accepted by most consumers.

The invention discloses a preparation method of an agar gel solution. The method is characterized by comprising the following steps: acidifying gracilaria; rinsing to be neutral; adding water into therinsed gracilaria, and heating to melt the gracilaria into glue; adding Ca (OH) 2 and / or CaO to enable the glue solution to be alkaline, and continuously heating; introducing CO2 to react with Ca (OH) 2 so as to generate CaCO3; and filtering to obtain an agar gel solution. The method is green and environment-friendly, can be used for preparing agar with high gel strength and high yield, and has relatively high application value.

The invention belongs to the field of foods, and specifically relates to seaweed-source low-sodium-content salt suitable for patients with hypertension and a preparation method thereof. The seaweed-source low-sodium-content salt suitable for the patients with hypertension comprises the following raw materials in parts by weight: 65-75 parts of edible salt, 15-25 parts of potassium chloride, and 25-35 parts of seaweed polysaccharides; and the seaweed polysaccharides are composed of the following ingredients in parts by weight: 35-55% of polysaccharide from gracilaria chouae, 25-35% of polysaccharide from eucheuma muricatum, and 15-30% of polysaccharides from nori. The prepared seaweed-source low-sodium-content salt suitable for the patients with hypertension has pure salty taste, as well asgreatly reduced sodium ion content; moreover, bitter and astringent taste of potassium salts can be covered without addition of any food additives, so that the salt has high adaptability. Thus, needsof modern healthy diet are met. In addition, the seaweed-source low-sodium-content salt prepared by the preparation method further has the functions of reducing blood lipid and preventing cardiovascular diseases; and thus, the salt is suitable for patients with hypertension as well as middle aged and old people in prevention of to eat.

Provided are a fenugreek cold-damp-dispelling fricassee seasoning and a preparation method therefor. The fricassee seasoning is prepared from traditional Chinese medicine powder, meat meal and chilli powder according to a weight ratio of 15:4:1. The traditional Chinese medicine powder is prepared from, by weight, 35-45 parts of fenugreek, 21-26 parts of Chinese banana root, 21-26 parts of bock greenbrier rhizome, 15-18 parts of maize, 15-18 parts of herba inulae cappae, 15-18 parts of pine mushroom, 15-18 parts of gracilaria, 8-13 parts of Jiangxi christina loosestrife herb, 8-13 parts of semen cassiae sopherae, 8-13 parts of cowpea, 8-13 parts of acronychia pedunculata and 8-13 parts of amaranthus viridis. The meat meal is prepared from rabbit meat, chicken, duck meat, goose meat and beef. The chilli powder is prepared from capsicum frutescens, shuanshuan pepper, pod pepper, capsicum annuum and ornamental pepper. The provided fricassee seasoning has functions of dispelling cold-damp, tonifying kidney yang and treating cold hernia after long-term consumption.

The invention relates to an automatic agar filling and cooling system. The system comprises a gracilaria gel boiling device, an agar condensing device, a to-be-condensed agar conveying belt and a gelframe, the middle section of the to-be-condensed agar conveying belt is arranged below a discharge hole of the gracilaria glue boiling device; the to-be-condensed agar conveying belt extends leftwardsand penetrates into a condensation chamber; the agar condensing device is installed in the condensation chamber and comprises an agar condensation conveying frame, a first lifting conveying frame anda second lifting conveying frame, the left end of the second lifting conveying frame is in butt joint with a condensed agar conveying belt, and the condensed agar conveying belt penetrates out of theleft side wall of the condensation chamber; the gracilaria gel boiing device comprises a cooking tank body, a stirring mechanism and a cooking mechanism, the gel boiling speed and the gel gelling speed are effectively increased, workers can be effectively prevented from being scalded by agar, and the production efficiency is greatly improved.

The invention discloses a preparation method of Gracilaria agar oligosaccharide promoting growth of Lactobacillus bulgaricus. The preparation method includes following steps: step 1, removing impurities of Gracilaria: cleaning, removing impurities of Gracilaria, drying, and placing Gracilaria in water bath at 80 DEG C for heating for 2 h; step 2, sterilizing at high temperature: sterilizing at 121 DEG C for 20 min; step 3, squeezing glue: using filter cloth to wrap Gracilaria, squeezing, collecting a polysaccharide solution, cooling, drying, smashing into granules, and bagging the granules for standby use; step 4, performing enzymatic treatment: dissolving cooled coarse polysaccharide in water to prepare a polysaccharide solution of 5g / L in concentration, adding agarase for treatment, performing enzymolysis for 20-1280 min to prepare an oligosaccharide solution, wherein enzyme adding quantity is 15% and enzyme activity is 300 U / mL; step 5, drying: freeze-drying the oligosaccharide solution or vacuum-drying the same to form solid powder to obtain the agar oligosaccharide. The preparation method can be used for Lactobacillus fermentation enterprises and dairy product production enterprises to produce high-activity Lactobacillus products and high-storability dairy products.

The invention relates to a method for preparing agarose. The method comprises the following steps: cleaning gracilaria, and carrying out alkali treatment, acidification treatment, bleaching treatment,gelatin boiling, filtration, cooling and solidification to obtain colloid; stirring and crushing the colloid; and adding water into the crushed colloid, heating, adjusting the pH value, adding an H2O2 solution for reaction, filtering, washing and repeatedly washing until no H2O2 is left in the colloidal particle sample and the pH value of the washing liquid is 7 after the reaction is finished, and freezing, dehydrating, drying and crushing the sample to obtain the agarose. According to the method, the content of sulfuric acid groups of agar can be remarkably reduced, the gel strength is improved, the electroendosmosis is remarkably reduced, the commercially available agarose index requirement is met, the reaction time is short, and the method is environmentally friendly.

The invention discloses a preparing method of gracilaria agaro-oligo saccharide for promoting streptococcus thermophilus growth.The method comprises the steps of 1, preparation of agarase crude enzyme; 2, decontamination of gracilaria, wherein gracilaria is cleaned, decontaminated, dried and heated in a water bath which is 80 DEG C for 2 h; 3, heating and sterilization, wherein heated gracilaria is heated again to 121 DEG C and sterilized for 20 min; 4, latex squeezing, wherein latex squeezing is conducted while gracilaria is still hot, gracilaria is wrapped in a filter cloth and squeezed, and polysaccharide solution filtered out through latex squeezing is collected, cooled, dried and smashed into particles to be bagged to be used, so that gracilaria crude polysaccharide is obtained; 5, enzyme treatment, wherein the agarase crude enzyme is added for treatment to prepare oligosaccharide solution; 6, drying, wherein freeze drying or vacuum drying is conducted on the oligosaccharide solution to obtain solid powder, so that the agaro-oligo saccharide is obtained.The method can be used in lactobacillus fermentation enterprises and dairy product production enterprises to produce high-activity lactic acid bacteria products and high-storability dairy products.

The invention discloses a gracilaria raw material liquid-to-material ratio prediction method, medium, equipment and device based on a neural network, and the method comprises the steps: obtaining historical data which comprises an agar index and a corresponding gracilaria raw material liquid-to-material ratio; training a neural network model according to the historical data to generate a gracilaria raw material liquid-to-material ratio prediction model; acquiring a to-be-predicted agar index, and inputting the to-be-predicted agar index into the gracilaria raw material liquid-to-material ratioprediction model, so as to enable the gracilaria raw material liquid-to-material ratio prediction model to generate a corresponding predicted gracilaria raw material liquid-to-material ratio according to the to-be-predicted agar index. According to the method, the gracilaria raw material and liquid material ratio can be effectively predicted before production, so that the consumption of water resources is reduced while the agar quality is guaranteed.

The invention relates to a gracilaria soaking and cooking system, which comprises a production workshop, and a clean water tank, an alkali liquor tank, a plurality of soaking tanks and a plurality ofcooking tanks which are arranged in the production workshop, the clean water tank is connected with a clean water output pipe, the alkali liquor tank is connected with an alkali liquor output pipe, and the other end of the clean water output pipe is connected with each soaking tank; the other end of the alkali liquor output pipe is connected with each soaking pool, the bottom of each soaking poolis connected with a clear water return pipe, an alkali liquor return pipe and a gracilaria conveying pipe, the other end of each gracilaria conveying pipe is connected and introduced into the cookingpool, a first stirrer is mounted in each soaking pool, and a second stirrer is mounted in each cooking pool; a discharge switch is arranged at the joint of the gracilaria conveying pipe and the soaking pool, and a first valve and a second valve are arranged at the joint of the clear water return pipe and the alkali liquor return pipe respectively. By means of the system, alkali liquor and clear water can be recycled, soaked gracilaria is directly conveyed into the cooking pools, the cooking pools and the soaking pools are used at the same time, and the processing number can be increased.

The invention relates to a gel cooking device for Gracilaria tenuistipitata. The gel cooking device comprises a cooking tank body, a stirring mechanism and a cooking mechanism, wherein the stirring mechanism is mounted at the top end of the cooking tank body and comprises a mounting bottom plate; a first driving assembly is mounted at the middle part of the mounting bottom plate; an output shaft of the first driving assembly downwards penetrates through the mounting bottom plate and is connected with a stirring shaft; detachable stirring blades are mounted on the stirring shaft; the cooking mechanism is arranged above the mounting bottom plate and comprises an annular steam outlet pipe, guide columns, a second driving assembly and a mounting plate; and an output shaft of the second drivingassembly downwards penetrates through the mounting plate and is connected with a lead screw. Since the guide columns are arranged between the mounting plate and the mounting bottom plate, energy consumption is reduced and resource utilization rate is increased; and the telescopic steam outlet pipe is matched with the stirring mechanism, so Gracilaria tenuistipitata which is being cooked can be heated more reasonably and evenly, the gel cooking process of Gracilaria tenuistipitata is accelerated, and gel cooking efficiency is improved.

The invention relates to a preparation method for improving the gel strength of Gracilaria agar by using glutaraldehyde, and belongs to the technical field of gel production. Agar with high gel strength is prepared by adding glutaraldehyde to the agar. The preparation method is to dissolve the powder of Gracilaria agar in hot water and configure it into an agar solution with a concentration of 1.5% (w / v). After boiling the glue for a certain period of time until it is completely dissolved, add hydrochloric acid catalyst at a certain range of temperature, add glutaraldehyde solutions of different concentrations, stir and react for several minutes, let it stand at room temperature to make the surface solidify, and then place it under constant temperature conditions. Gel for several hours, and measure its gel strength with reference to the method in the national standard GB1975-2010. The invention has the advantage that the gel strength of the Gracilaria agar is improved by a chemical modification method, and the obtained agar can be applied to the fields of daily chemical industry, bioengineering and the like, and has simple operation and low production cost.