The invention discloses heterogeneous micro-patterned hydrogel and a preparation method and application thereof, and the method comprises the following steps: S1, dissolving
agar to obtain an
agar solution, and injecting the
agar solution into a mold to prepare
agar gel; s2, mixing methacrylated
gelatin and a
photoinitiator to obtain a pre-
polymerization solution, and soaking the agar glue in the pre-
polymerization solution; s3, covering the soaked
agar gel with a
mask plate, and illuminating the
agar gel, so as to obtain heterogeneous micro-patterned hydrogel; wherein the content of agar in the agar solution is 0.5-5 wt%, and the content of methacrylated
gelatin in the pre-
polymerization solution is 3-30 wt%. According to the hydrogel provided by the invention, the chemical compositions of the adhesion area and the anti-adhesion area are different, so that the
cell adhesion capacities and swelling properties of the two areas are different, and the difference of the swelling properties further enables the adhesion area to form bulges compared with the anti-adhesion area, so that the cooperative regulation and control of the chemical compositions and the
physical structure are realized; the
cell behavior can be accurately regulated and controlled.