86 results about "Agar gel" patented technology

The invention discloses a reduced graphene oxide @ZIF-8 composite-film-modified electrode, a preparing method thereof and application of the electrode in simultaneously detecting naphthol isomers. According to the preparing method, a 2-methylimidazole solution is added into a reaction tube containing a graphene oxide agar gel matrix, a natural diffusion and penetration method is adopted for preparing a graphene oxide @ZIF-8 nano-compound, then a sample is divided into three layers according to the diffusion depth, afterwards, thermal reduction is conducted to prepare a reduced graphene oxide @ZIF-8 nano-compound, and then the corresponding modified electrode is prepared. The obtained modified electrode has the advantages of large effective area, many active sites, good dispersity and the like. Reduced graphene oxide and ZIF-8 overcome mutual defects, the synergistic effects of the reduced graphene oxide and ZIF-8 in the aspect of improving direct electrochemistry and electro-catalyticperformance are achieved, and the conductivity and catalytic performance of the modified electrode are improved. The obtained modified electrode simultaneously detects the high sensitivity of the naphthol isomers and has the advantages of being low in detection limit, wide in detection range, high in response speed and the like.

A multimodality imaging phantom is disclosed which is useful for calibrating devices for imaging vascular conduits. The phantom is compatible with X-ray, ultrasound and magnetic resonance imaging techniques. It allows testing, calibration, and inter-modality comparative study of imaging devices, in static or dynamic flow conditions. It also provides a geometric reference for evaluation of accuracy of imaging devices. The tissue-mimicking material is preferably an agar-based solidified gel. A vessel of known desired geometry runs throughout the gel and is connected to an inlet and outlet at its extremities for generating a flow circulation in the vessel. Said phantom also contains fiducial markers detectable in the above-mentioned modalities. The markers are preferably made of glass and are embedded in a layer of agar gel containing a fat component. The markers are implanted at precise known locations to allow identification and orientation of plane views, and they can be used for calibration, resealing and fusion of 3D images obtained from different modalities, and 3D image reconstruction from angiographic plane views. Also disclosed is a process for manufacturing said phantom.

The invention discloses a group I type 4 aviadenovirus strain WZ. The strain has a preservation number of China Center for Type Culture Collection (CCTCC) No: V201662, is named as group I type 4 aviadenovirus strain WZ in a classified way, and is preserved in December 14, 2016; a group I type 4 viral vaccine of the aviadenovirus is prepared from the inactivated group I type 4 aviadenovirus strain which is taken as an active ingredient; the invention also provides application of the group I type 4 aviadenovirus strain WZ in preparation of an agar gel precipitating antigen, a hemagglutination inhibition (HI) antigen and positive serum antigen reagent which are used for diagnosing the group I type 4 virus of the aviadenovirus, and preparation of egg yolk antibody and antiserum which are used for treating the group I type 4 virus of the aviadenovirus. After the vaccine prepared by using the group I type 4 aviadenovirus strain is used for immunizing, the toxin attacking protection rate for the group I type 4 aviadenovirus strain WZ reaches 90-100%. As a vaccine strain with good manufacturing effect, the group I type 4 aviadenovirus strain WZ can be used for preventing avian inclusion body hepatitis and pericardial effusion syndrome, and also can be used for virus identification and epidemiological investigation.

The invention relates to a mononuclear copper complex and a preparation method and application thereof. Chemical formulas of the mononuclear copper complex are respectively [CuLCl](ClO4)(1) and [CuL(acac)](PF6)(2), wherein L is N,N-(2-pyridyl)-1-(1-naphthyl) ethylamine, acac is acetylacetone, and PF6 is hexafluorophosphate radical. The complex (1) crystal is of an orthorhombic system, a space point group is Pbca, cell parameters are described in the specification; the complex (2) crystal is of a monoclinic system, a space point group is P2(1)/c, and cell parameters are described in the specification. A plurality of spectroscopic methods represent that the mononuclear copper complex has a strong bonding function for CT-DNA (Circulating Tumor-Desoxvribose Nucleic Acid); agar gel electrophoresis experiments prove that the mononuclear copper complex has a remarkable cutting effect on pBR322DNA by using an oxidation cutting principle; MTT (Methyl Thiazolyl Tetrazolium) experiments prove that the mononuclear copper complex has a better anti-tumor activity to multiple kinds of cells, and can be used as a potential anti-tumor medicine.

The invention discloses an aryl sulfatase gene, a protein encoded by the aryl sulfatase gene as well as an immobilization method and application of the protein. The immobilization method and application comprise the following steps of firstly expressing the aryl sulfatase gene of pseudoalteromonas carrageenovora sp in escherichia coli and purifying to obtain a recombinant aryl sulfatase; by adopting carboxyl-functionalized Fe3O4 magnetic nanoparticles as carriers and glutaraldehyde as a cross-linking reagent and immobilizing the free recombinant aryl sulfatase to obtain the immobilized recombinant aryl sulfatase; treating agar with the immobilized recombinant aryl sulfatase, carrying out shaking reaction for 1 hour at 40 DEG C and measuring the physical properties of agar such as gel strength, the content of sulfate groups and the like. The immobilized recombinant aryl sulfatase disclosed by the invention can be repeatedly used, still maintains more than 60% of the enzyme activity after being repeatedly used for eight times and shows good operating stability and feasibility of practical application.

The invention relates to a method for improving gracilaria agar gel strength and belongs to the technical field of food production. The method aims to prepare high-strength agar by adding a microorganism enzyme namely transglutaminase into agar and specifically includes: dissolving agar powder in hot water to prepare 1.5% agar solution, boiling agar for a certain time until the agar is completely dissolved, adding transglutaminases of different concentrations within a certain temperature and pH range, stirring for reaction for a few minutes, standing at the room temperature to enable the surface to be solidified, placing at constant temperature for gelling for a few hours, and measuring the gel strength. The gel strength of the agar is evidently improved when addition amount of the transglutaminases is 0.01%-1.0% (g/g, relative to dry weight of agar powder). The method has the advantages that the agar is assuredly edible by using the biological method for improving gel strength, nontoxic, harmless and pollution-free, and the method is simple in operation and low in production cost.

The invention relates to a simulating method and a simulating device of wetland plant rhizosphere anaerobic environment. The simulating method of the wetland plant rhizosphere anaerobic environment comprises spreading an agar gel layer with cane sugar on the bottom portion of bottom mud, injecting bottom mud on the agar gel layer and forming certain bottom mud anaerobic environment through the gradual degradation of the cane sugar to consume the oxygen in the bottom mud. The simulating device is composed of a plant planting column, a rhizosphere layer and an anaerobic bottom mud column, the plant planting column, the rhizosphere layer and the anaerobic bottom mud column are fixedly connected through polyvinyl chloride (PVC) flanges, screw holes and bolts, the thickness of the rhizosphere layer is 1mm to 2 mm, and the rhizosphere layer is filled with a thin layer of bottom mud in a using process. The simulating device is simple in manufacturing and the method is simple in application, and experiment simulation effect is good.

The invention relates to agar gel microspheres and a preparation method, which belongs to the technical field of biochemical separating and purifying medium preparation. The agar gel microspheres take agar raw spheres as a raw material, and can be prepared through twice crosslinking, a long cross-linking agent I is 1,4-butylene-glycol twice-shrunk glyceryl ether; and a short cross-linking agent II is chloropropylene oxide. The preparation method comprises the following steps: washing agar raw spheres and filtering to a dry state, adding the cross-linking agent 1,4-butylene-glycol twice-shrunk glyceryl ether, uniformly mixing; stirring under mechanical stirring and standing at room temperature overnight; adding a NaOH solution drop by drop and adding NaBH4, reacting for 4 hours and performing mechanical stirring, heating a system to the temperature of 39 DEG C, reacting for 4 hours under the temperature; adding 2ml of cross-linking agent chloropropylene oxide in the system drop by drop, heating to the temperature of 43 DEG C, mixing for half hour, mixing while performing mechanical stirring; adding NaOH drop by drop and adding NaBH4 for uniformly mixing, continuously reacting for 12 hours at the temperature of 43 DEG C; continuously adding the NaOH solution drop by drop, adding chloropropylene oxide for mechanical stirring and reacting , and then washing for multitime to neutrality. The agar gel microspheres have the advantages of stronger rigidity, stable structure and better performance.

The invention relates to a duck tembusu virus (DTMUV) disease immunotherapy preparation and preparation method thereof, which belong to the field of bio-pharmaceuticals. The preparation method of the DTMUV disease immunotherapy preparation comprises the following steps of: culturing seed viruses for producing a tembusu virus strain on a large scale, and harvesting a virus liquid; deactivating the collected virus liquid, concentrating, adding the deactivated and concentrated virus liquid into an adjuvant, and emulsifying to obtain an immune antigen; immunizing laying ducks by using the immune antigen, randomly collecting blood and separating serum after last immunization, and measuring the valence of an anti-DTMUV antigen through an Agar Gel Precipiti (AGP) test till the valance of the antigen is qualified; and collecting eggs laid by an immunized qualified duck group, and preparing into a high-immunity egg yolk antibody with the conventional method. The DTMUV disease immunotherapy preparation contains a DTMUV high-immunity egg yolk antibody. The DTMUV disease immunotherapy preparation prepared with the method has the advantages of good treatment effect, safety, reliability and low cost, and is suitable for large-scale industrial production.

The present invention is drawn to a method for de-staining a substrate that is stained with a dye by bringing the substrate into contact with a de-staining composition and absorbing the dye from the substrate with the de-staining composition, wherein the de-staining composition is a charcoal or ion-exchange resin absorbent incorporated in a semi-solid matrix which is a polyacrylamide or agar gel. With the method of the invention a stained substrate may be stained and de-stained in a single step. The present invention is further drawn to an apparatus for performing the method of de-staining the substrate.

The invention relates to the field of air purifying, and discloses an indoor air sterilizing and purifying filter element material and a preparation method. The preparation method comprises the following preparation process with the steps of (1) mixing nanometer titanium dioxide, activated carbon powder, diatomite powder, polyacrylonitrile, polyamide, acetic acid and glycerol to form an emulsion,and performing electrostatic spinning to form a net, so as to obtain a plate-shaped core material; (2) adding nanosilver and polyiodide into silicon sol, so as to obtain bacterium inhibiting and sterilizing powder; (3) adding agar gel and polyvinyl alcohol into the bacterium inhibiting and sterilizing powder to form a gel-shaped matter, impregnating the plate-shaped core material, and drying, so as to obtain the indoor air sterilizing and purifying filter element material. The prepared indoor air sterilizing and purifying filter element material has the advantages that by using the nanosilverand the polyiodide as sterilizing agents, the sterilizing ability is strong, and the safety is good; the formed sterilizing layer can completely coat the core material, so that the effective contact with bacteria is realized; the falling is completely avoided, so that the sterilizing effect is better.

The invention discloses a method for detecting sperm deoxyribonucleic acid fragments and film integrality. The method comprises the following steps of: (1) preparing hypotonic solution; (2) preparing an eosin-Giemsa stain; (3) performing a simple sperm hypotonic swelling experiment; (4) performing centrifugalization on the sperm obtained by hypotonic treatment in the step (3), discarding supernate and diluting the sperm; (5) mixing the diluted sperm in the step (4) with agar gel in a certain ratio and performing incubation for 5 minutes to obtain the formulated sperm; (6) placing the formulated sperm in the step (5) on a glass slide; (7) soaking the glass slide in the solution of hydrogen chloride (HCl); (8) adding trihydroxymethylaminomethane, dithiothreitol and sulfonated succinic acid N-lauryl-based monamide disodium salt on the glass slide to soak to obtain a sample sheet; (9) washing the sample sheet in the step (8) with buffer solution; (10) dehydrating the sample sheet; and (11) drying the dehydrated sample sheet obtained by the step (10) and adding the dehydrated sample sheet into the stain for dyeing.

The invention relates to a method for preparing a yolk immune globulin nanometer liposome for preventing and controlling infectious bursal disease, and belongs to the technical field of medicine preparations of animals. The method for preparing the yolk immune globulin nanometer liposome for preventing and controlling chicken infectious bursa of Fabricius comprises the following steps of: 1, preparing a yolk antibody, namely performing vaccine inoculation on chickens by a multi-time and alternate immune method, and when the agar gel precipitate test (AGPT) detection valence of the yolk antibody is over 1:64, collecting yolk; 2, separating immune globulin, namely diluting yolk suspension by using acetic acid-sodium acetate buffer solution, stirring, standing, centrifuging and reserving supernate; and 3, preparing a liposome, namely preparing a liposome membrane by a membrane dispersion method, adding the yolk immune globulin, rotating and stirring to form a yolk immune globulin liposome capsule, and reforming the formed liposome capsule by using a homogenizer to obtain the nanometer liposome. In the method, the yolk immune globulin is enveloped, and the method has the advantages of simple process, economy, practicability, stable economic performance, good treatment effect and the like.

The invention discloses a pullorum agar gel precipitating antigen as well as a preparation method and an application thereof, and belongs to the technical field of veterinarian diagnosis. Salmonella pullorum SP8441 and salmonella pullorum SP9905 are subjected to recovery and passage respectively to form a seed bacterium liquid, then the seed bacterium liquid is inoculated with a solid culture medium for proliferation, after a culture solution is removed centrifugally, bacterial protein is extracted with a lysis buffer, and the pullorum agar gel precipitating antigen is prepared. The technological method is simple, reasonable, scientific, stable in production and low in cost, the prepared pullorum agar gel precipitating antigen product has the advantages of high sensitivity and high specificity, the pullorum purification process can be simplified, the detection application range can be enlarged, and the agar gel precipitating antigen is relatively ideal in pullorum detection.

The invention relates to a production method for producing disposable top grade tableware by utilizing rice straws and wheat straws, aiming at improving comprehensive utilization of the rice straws and the wheat straws. The production method for producing the disposable top grade tableware by utilizing agricultural straws comprises the following processes of: sorting, drying, fiber separating, cooking, bleaching, drying and carding to prepare a high-quality raw fiber material; and then placing the raw fiber material, talcum powder, agar gel and starch glue into a mixing agitator in proportion, mixing to be uniform, carrying out hydraulic pressing by a grinding tool, stoving, renovating and checking, then placing the mixture into a disinfection cabinet, and sterilizing, disinfecting and packaging to produce the disposable top grade tableware. The disposable top grade tableware produced by the production method disclosed by the invention has the characteristics of no toxicity and smell, high temperature resistance, fair and clear appearance, no leakage, no crack, high strength, high stiffness, no objectional odour, no pollution and easy degradation, belongs to green disposable tableware appliances and has a favorable guarantee on the human health.

The invention provides a preparation method of a sulfur modified metal hydroxide electrode material and belongs to the technical field of preparation of electrode materials. According to the preparation method, firstly, NaOH/agar gel is prepared; OH- and gel have a very strong binding force so that the dispersion of the gel on a solid-liquid interface is easy to control and the formation of ultrathin flower-shaped nano-sheet metal hydroxide is facilitated; then Na2S/agar gel is synthesized, and NiS/CoS with a smaller solubility product is generated on the surface of the hydroxide at room temperature through an ion exchange method, and furthermore, the ultrathin nano-flower sulfur modified metal hydroxide electrode material with excellent performance is formed. The preparation technology provided by the invention has the characteristics of simplicity, economical efficiency and energy saving; dangers of causing toxic gas of a traditional sulfide synthesis technology are abandoned, and the preparation method has the characteristics of greenness and environment protection; meanwhile, a compound supercapacitor prepared from the electrode material has the advantages of low cost, simple production technology and very high energy density and good circulation stability, so that the preparation method is suitable for industrial application.

The invention discloses a method for distinguishing different plant arising from plant mitochondrion sequence which comprises the steps of, extracting plant genome DNA and mitochondria genome DNA, searching biological mitochondria genome sequence or gene order of several modes from the internet websites, carrying sequence comparison by sequence analyzing software, digging conservative sequences, designing a series of primers by utilizing a primer design software, using the extracted genome DNA and mitochondria DNA as template to carry out polymerase chain reaction to the designed primers, using agar gel electrophoresis for result detecting and analysis to the polymerase chain reaction.

The invention discloses a preparation method of an agar gel solution. The method is characterized by comprising the following steps: acidifying gracilaria; rinsing to be neutral; adding water into therinsed gracilaria, and heating to melt the gracilaria into glue; adding Ca (OH) 2 and/or CaO to enable the glue solution to be alkaline, and continuously heating; introducing CO2 to react with Ca (OH) 2 so as to generate CaCO3; and filtering to obtain an agar gel solution. The method is green and environment-friendly, can be used for preparing agar with high gel strength and high yield, and has relatively high application value.

The invention relates to a DGT (diffusive gradients in thin films) enrichment film for measuring bio-activity Hg (II) of a paddy soil solution as well as a preparation method thereof. The material comprises the followings: a bonding material for adsorbing Hg (II) is modified nanometer sulfydryl metal double-layer hydroxide; a supporting material is modified agar gel; and a dispersing agent is ethanol. A Hg (II) enriched DGT film is prepared by the following steps: putting the materials into ultrapure water with the temperature of 80 DEG C according to the proportion requirement, mixing completely and uniformly, injecting into a glass pane groove with the temperature of 60 DEG C until the groove is sealed, flatly placing, cooling to 25 DEG C and taking out to prepare the Hg (II) enriched DGT film. The enriched film material can enrich the Hg (II) efficiently and rapidly, can be completely dissolved within 30 minutes after 5 ml of concentrated hydrochloride acid or 1M of nitric acid is added, and overcomes the defects that the conventional DGT enriched film for adsorbing the Hg (II) is digested incompletely, is difficult in laboratory preparation, has a low adsorption property in theHg (II) and is difficult to buy.