13 results about "Agar gel" patented technology

The invention discloses heterogeneous micro-patterned hydrogel and a preparation method and application thereof, and the method comprises the following steps: S1, dissolving agar to obtain an agar solution, and injecting the agar solution into a mold to prepare agar gel; s2, mixing methacrylated gelatin and a photoinitiator to obtain a pre-polymerization solution, and soaking the agar glue in the pre-polymerization solution; s3, covering the soaked agar gel with a mask plate, and illuminating the agar gel, so as to obtain heterogeneous micro-patterned hydrogel; wherein the content of agar in the agar solution is 0.5-5 wt%, and the content of methacrylated gelatin in the pre-polymerization solution is 3-30 wt%. According to the hydrogel provided by the invention, the chemical compositions of the adhesion area and the anti-adhesion area are different, so that the cell adhesion capacities and swelling properties of the two areas are different, and the difference of the swelling properties further enables the adhesion area to form bulges compared with the anti-adhesion area, so that the cooperative regulation and control of the chemical compositions and the physical structure are realized; the cell behavior can be accurately regulated and controlled.

The invention belongs to the technical field of agar extraction and processing, and discloses a construction method and application of a mathematical model for determining agar gelation temperature. The construction method of the mathematical model for determining the agar gelation temperature comprises the following steps: measuring the time of the gelation process and the generation rate of ClO3 <-> at the specific gelation temperature, and carrying out unary multi-term fitting on the time and the generation rate of ClO3 <->; according to the fitting result, the time of the gelation process is determined, the ClO3 <-> concentrations at different gelation temperatures are measured at the time of the gelation process, then the gelation temperatures and the ClO3 <-> concentrations are subjected to unary multi-term fitting, a mathematical model of the agar gelation temperature is constructed, and the agar gelation temperature is calculated. And taking the temperature corresponding to the lowest point of the model curve as the gelation temperature. According to the method, the gelation temperature suitable for producing the low-toxicity byproduct agar is determined through construction of a mathematical model.

The present invention relates to a nucleic acid electrophoresis test gel, which comprises a liquid agar gel and a solution of the acrylamide methylenebisacrylamide (Acr-Bis). By combining the liquid agar gel with the Acr-Bis solution, the present invention can improve the separation resolution of a gel formed from agar during nucleic acid electrophoresis, thereby making the properties of the nucleic acid electrophoresis test gel similar to those of commercially available agarose gel, and achieving good separation resolution for DNA fragments to be subjected to gel electrophoresis during nucleic acid electrophoresis tests.

The application provides a method for detecting plant growth hormone-like substances in sludge stabilization products, comprising the following steps: contacting the root system of a plant with a sludge extract solution added with CaCl2 to activate a messenger; transferring the plant treated by the activated messenger to an agar gel layer plate added with bromocresol purple indicator; observing the color development of the agar gel layer plate during the culture process; and judging the content of the plant growth hormone-like substances in the sludge extract solution according to the color development. The method for detecting plant growth hormone-like substances in sludge stabilization products provided by the application is based on the acidification degree of the root system of a plant, takes bromocresol purple as an indicator, and combines color development with a plant water culture experiment, so that simple, rapid and low-cost qualitative detection of the plant growth hormone-like substances in sludge stabilization products is realized.

The invention belongs to the field of preparation of biopolymer materials, and particularly relates to a preparation method of agar gel microspheres, the agar gel microspheres prepared by the preparation method and application of the agar gel microspheres. Aiming at the problems of large particle size range, poor particle size uniformity and the like of the existing hydrogel microspheres, the invention provides the preparation method of the agar gel microspheres, which comprises the following steps: (1) mixing a bacterial suspension and an agar aqueous solution, and carrying out primary preheating to obtain a water phase; (2) an oily solvent and a surfactant are mixed and subjected to secondary preheating, an oil phase is obtained, and the surfactant is selected from at least two of a span series surfactant, a Tween series surfactant, Triton X-100 and ABIL EM 90; and (3) adding the water phase into the oil phase, stirring at 45-75 DEG C, curing, and demulsifying to obtain the agar gel microspheres. The hydrogel microspheres prepared by the method are appropriate in particle size range and good in particle size uniformity.

The utility model discloses a rollable extraction type puncher based on porous agar gel, which comprises an extraction type puncher connecting total component, a supporting plate is fixedly connected to the side surface of the extraction type puncher connecting total component, an output plate is arranged on the side surface of the extraction type puncher connecting total component, and the output plate is fixedly connected with the supporting plate. And a piston plate is clamped on the surface of the inner ring of the output plate in a limiting manner. After the device is inserted, a user needs to use and insert a second pull ring, so that the user can block other openings of the output plate so as to ensure the sealing performance of the whole use of the extraction type perforating machine connecting total component, and when the user inserts the output plate, the user needs to screw a knob handle, so that the user can conveniently insert the second pull ring into the output plate. In this way, a user can unscrew a threaded rod on a nut, so that the user can adjust the position of a limiting insertion rod on a connecting lantern ring, and the user can efficiently punch agar gel.

The invention belongs to the technical field of agar extraction and processing, and discloses a method for determining agar gelation pH and time and application. The method for determining the gelation pH and the gelation time of the agar comprises the following steps: collecting the gelation pH and the gelation time in the production process of agar products in a factory, and measuring the gel strength of the agar to form a data set; utilizing machine learning to predict and optimize the agar gel strength to obtain gelation pH and time range; the gelation time is determined according to the relation between the generation rates of ClO3 <-> and ClO4 <-> under different gelation pH values and the time; and under the gelling time, constructing a relation model between the gelling pH and the concentrations of ClO3 <-> and ClO4 <-> to determine the gelling pH. According to the method, rich data accumulated in large-scale production of factories and mechanism experiment results accurately developed in laboratories are fused, and through deep cooperation and interactive verification of the rich data and the mechanism experiment results, the gel strength is optimized and improved, and the chlorate generation process is effectively controlled.

The present application relates to the technical field of fabric testing, in particular to an experimental test method and device for determining the impact resistance of fabric, comprising the following steps: S1: initial calibration, installing the fabric to be tested on a fabric frame, adjusting the tension of the fabric to be tested to a set value by a first electric push rod, starting the electromagnetic suction table compression spring to the energy storage state, launching the projectile to impact the pressure sensor array module under no load, repeating 3 times, obtaining the impact force calibration value, and synchronously calibrating the speed sensor, S2: test position switching, injecting liquid agar gel into the transparent gel cylinder. Through the unique spring system, the electromagnetic suction table is moved by using the linear transmission module, the spring stretching amount is accurately controlled, and the precise adjustment of the projectile launching speed is realized, which enables the simulation of various speed impacts when testing the impact resistance of the fabric.

The invention relates to a method for separating and purifying ascospores of cordyceps sinensis strains, and belongs to the field of ascospore strain separation and culture. The method comprises the following steps: firstly, removing impurities such as bacteria and fungi through gradient forward flushing and efficiently recovering target ascospores through reverse flushing by virtue of a three-stage gradient volume flushing, filtering and purifying process; performing primary culture and isolated culture on the filtered and purified ascospores, adding chloramphenicol into a culture medium to inhibit bacterial pollution, and simulating an ascospore primary environment to perform constant-temperature and constant-humidity culture; and after the bacterial colony is visible to the naked eyes, transferring and germinating the monospores into the culture medium by adopting a metal agar gel puncher, and continuously culturing to obtain the pure cordyceps sinensis mycelia developed from the single ascospores. The invention provides a separation and purification method for efficiently, purely and controllably separating the cordyceps sinensis fungus ascospores, effectively breaking the dormancy of the cordyceps sinensis fungus ascospores and remarkably improving the germination rate, and a high-purity and active cordyceps sinensis fungus pure culture can be obtained.

The application belongs to the field of biological polymer material preparation, and particularly relates to a preparation method of agar gel microspheres, agar gel microspheres prepared by the method and application. In view of the problems of the existing hydrogel microspheres, such as a large particle size range and poor particle size uniformity, the application provides a preparation method of agar gel microspheres, comprising the following steps: (1) mixing bacterial suspension and agar aqueous solution, and preheating once to obtain an aqueous phase; (2) mixing an oily solvent and a surfactant, and preheating twice to obtain an oil phase, wherein the surfactant is selected from at least two of a Span series surfactant, a Tween series surfactant, Triton X-100 and ABIL EM 90; (3) adding the aqueous phase into the oil phase, stirring and treating at 45-75 DEG C first, and then solidifying and demulsifying to obtain the agar gel microspheres. The hydrogel microspheres prepared by the method have a suitable particle size range and good particle size uniformity.

The invention provides an extraction method for improving agar performance based on modified perlite, the method comprises the following steps: carrying out acid-heat-ultrasonic synergistic modification on perlite, modifying the perlite by optimizing a porous structure and modifying a surface functional group, and introducing the modified perlite as a filter aid core into an extraction process, the gel strength of the obtained agar reaches 1571.26 g / cm < 2 >, and the transparency is 89.08. Compared with the original process, the gel strength is improved by 55.8%, and the transparency is improved by 43.64%; the method is green, efficient and suitable for the fields of high-end food, medicine and biological materials.

The utility model relates to the technical field of agar gel punching, in particular to an agar gel negative pressure hole forming device which comprises a hole forming device body connected with negative pressure equipment, and the hole forming device body comprises a material storage barrel and an installation barrel. An opening is formed between the opposite ends of the storage barrel and the mounting barrel, and the storage barrel and the mounting barrel are matched for use through a connecting part at the opening; wherein the top of the storage barrel communicates with a connector, a filter screen is arranged at the position, located at an opening of the connector, in the storage barrel, and a plurality of punching pipes are detachably connected to the bottom of the mounting barrel through fixing pieces, so that quick disassembly and assembly can be conducted, and the filter screen can be conveniently disassembled to clean broken gel blocks; and the punching pipe can be independently replaced through the fixing piece, the situation that normal punching of the gel block is affected due to the fact that the punching pipe is tedious to replace after long-time use is avoided, and in addition, the situation that waste is caused due to the fact that a plurality of punching pipes need to be replaced at the same time can be avoided.

The present invention defines a process for forming a chewable oral dosage form, such as a gum, with a pleasant texture and flavor. Specifically, the invention comprises the process of forming an oral dosage form comprising a first step of hydrating the agar-agar gelling agent and locust bean gum; a second step of incorporating components of the agar matrix; a third step of incorporating the active ingredient; a fourth step of cooking the mixture; a fifth step of flavoring; a sixth step of molding and gelling; and a seventh step of demolding, degreasing, and drying. Likewise, the oral dosage form according to the present invention comprises an agar-agar matrix in combination with gums such as locust bean gum and / or sugar; glycerin; inulin; flavorings; citric acid; a coating; and water to complete the composition, which includes the active ingredients.