The invention relates to a method for preparing a
yolk immune
globulin nanometer
liposome for preventing and controlling
infectious bursal disease, and belongs to the technical field of
medicine preparations of animals. The method for preparing the
yolk immune
globulin nanometer
liposome for preventing and controlling chicken infectious
bursa of Fabricius comprises the following steps of: 1, preparing a
yolk antibody, namely performing vaccine
inoculation on chickens by a multi-time and alternate immune method, and when the
agar gel precipitate test (AGPT) detection valence of the yolk
antibody is over 1:64, collecting yolk; 2, separating immune
globulin, namely diluting yolk suspension by using
acetic acid-
sodium acetate
buffer solution, stirring, standing, centrifuging and reserving supernate; and 3, preparing a
liposome, namely preparing a
liposome membrane by a membrane dispersion method, adding the yolk immune globulin, rotating and stirring to form a yolk immune globulin liposome
capsule, and reforming the formed liposome
capsule by using a
homogenizer to obtain the nanometer liposome. In the method, the yolk immune globulin is enveloped, and the method has the advantages of simple process, economy, practicability, stable economic performance, good
treatment effect and the like.