49 results about "Ascospore" patented technology

The invention discloses a chaetomium globosum FSR-74. The collection unit of the chaetomium globosum FSR-74 is China General Microbiological Culture Collection Center, the collection date of the chaetomium globosum FSR-74 is September 19, 2014 and the collection number of the chaetomium globosum FSR-74 is CGMCC No. 9690. The strain is relatively slow in growth and has light brown colonies and an irregular wave-like edge when subjected to plate culture at 25 DEG C in a potato dextrose agar (PDA) culture medium. FSR-74 ascocarps are supergene and fixed on the surface of a matrix by rhizoids; top hair is wavy, brown and has intervals, and dense wart points are arranged on the surface; 8 ascospores are arranged in an ascus; the ascospores are shaped like lemons and have single apical bud holes. The chaetomium globosum FSR-74 has an efficient broad-spectrum bacteriostatic effect against main pathogenic bacteria causing ginseng rust rot, blight, sclerotinia rot, rust rot, blackspot, sheath blight and gray mold. The invention further provides a biological control mechanism and application of the chaetomium globosum FSR-74 in prevention and treatment of plant fungal diseases, as well as application in preparation of a microbial agent for preventing and treating the plant fungal diseases.

The invention belongs to the technical field of agricultural microorganisms, in particular to Bacillus velenzensis and application thereof to biological control. The Bacillus velenzensis disclosed bythe invention is Bacillus velezensis Bv-6, and a preservation number is CCTCC NO:M20191106. The Bacillus velenzensis fermented solutions or powder can effectively inhibit growth of mycelia of important pathogens (Sclerotinia sclerotiorum(Lib.)de Bary, Botrytis cinerea Pers. and Blackleg) on rapes, and germination of conidiophores and ascospores; and through seed soaking treatment of the fermentedsolutions, the seeds of the rapes can be normally germinated and grown, and the edisease resistance of plants can be improved. In addition, the Bacillus velenzensis has the characteristics of being high in proliferation speed, high in stability, high in temperature resistance and free of toxicity. Therefore, the Bacillus velezensis strain Bv-6 has the potential of developing and utilizing the function of the biological control.

The invention provides a method for the genetic transformation of agrobacterium tumefaciens mediated sclerotinia sclerotiorum. The method comprises the following steps: mixing and co-culturing the agrobacterium tumefaciens containing binary vectors and the protoplasts of the sclerotinia sclerotiorum; and screening by using antibiotics to obtain transformants, the transformation efficiency of the method reaches 1 / 5.9*104 protoplasts. By using the protoplasts of the sclerotinia sclerotiorum as the receptor and using the agrobacterium tumefaciens as the mediator, the method of the invention overcomes the problem of the difficult genetic transformation of sclerotinia sclerotiorum, which is resulted from the multinucleate hypha cells and the difficulty in obtaining a large amount of ascospore, and has the advantages of simple method and stable transformation efficiency. Meanwhile, the invention further provides a simple and effective method for preparing the protoplasts of sclerotinia sclerotiorum.

The invention discloses a Saccharomycopsis fibuligera and application thereof. The morphological characteristics are as follows: after the bacterial colony formed by the Saccharomycopsis fibuligera is cultured on a PDA (potato dextrose agar) culture medium at 28 DEG C for 2 days, the bacterial colony diameter is 3-5mm; the bacterial colony is circular, white and nontransparent and feels like short down; the edge of the bacterial colony is in the shape of etched teeth; and the bacterial colony can be easily picked up. The cell is in the shape of an egg-like circle; the size of the cell is 2.35-3.97 mu m, and the length is 4.66-5.42 mu m; and the cell can form pseudohypha and produce ascospores. The Saccharomycopsis fibuligera can produce isoamyl alcohol in Maotai-flavor liquor.

The invention discloses a truffle microcapsule and a preparation method and an application thereof, which belong to the technical field of preservation and processing of wild edible fungi. Truffle slurry obtained by crushing mature truffle is used as a core material and sodium alginate and chitosan are used as wall materials to prepare a microcapsule, and freeze drying is carried out on the microcapsule to obtain the truffle microcapsule. By adopting the method disclosed by the invention, such important biological active components as alpha-androstanol in the truffle are avoided from being oxidized, the loss of volatile aroma components is small, the effective components of the truffle can be furthest reserved, the storage period is long, and the truffle is conveniently transported and used. Two types of truffle core materials are obtained by adjusting the gap between a stator and a rotor of a colloid mill, the prepared microcapsule has two completely different usages, hyphae cells with large particle sizes contain integral ascospores and still have activity, so that the hyphae cells can be used as indoor or outdoor inoculants of mycorrhizal seedlings; spores of the hyphae cells with small particle sizes are broken, so that the nutritional ingredients are more beneficial for human body adsorption, and the truffle microcapsule is mainly used as a food additive and used for making truffle paste, truffle soup, seasoner, etc.

The invention relates to a fast appraisal method of Cordyceps militaris fertile strains, which belongs to the edible fungus cultivation field. Single colonies which are sprouted by ascospore are selected, 100 single colonies are selected each time to be put under the constant temperature of 20 DEG C for culture for 16 days, with indoor natural lighting and the air humidity of 70 percent; strong strains with above 20 grass teeth, which grow uniformly, and have faint yellow colonies, golden yellow grass teeth, even growing trend, and no abnormalities are picked up, examined and confirmed by a microscope to obtain the fertile strain. The fertile strains are propagated and cultured for one time, the propagated strains are reproduced for 5 to 8 days by using a liquid media, and a mycelium pellet formed in the liquid becomes the strains for production. The method of the invention has short appraisal time and high accuracy. The time from the colony selection to the appraisal result only lasts for 20 days, more production cycles suitable for the two generation can be won for the Cordyceps militaris production, thereby further reducing the production cost, increasing the production speed, and improving the quality and the output of the Cordyceps militaris.

The invention provides a Rhodotorulasp.2-14 China general microbiological culture collection (CGMCC) NO. 4445. The Rhodotorulasp.2-14 is circular or oval, the size of the Rhodotorulasp.2-14 is 4.5-5.1*6.3-7.2mu m, and the reproduction mode is budding and is free of the formation of pseudohypha, ascospore and ballistospore. The invention further discloses a method for producing trehalose using the Rhodotorulasp.2-14. The trehalose of a bacterial strain is high in yield, simple in a culture medium and cultivation process, and energy-saving and environment-friendly in extraction process. The method for producing the trehalose has the advantages of being simple and practical, and low in cost, thereby being easy to popularize and apply in the industrial production.

The invention discloses a method for effectively preventing and controlling mulberry sclerotiniose. The method includes the steps of firstly, deeply plowing soil of a greenhouse mulberry garden in the period from sclerotium initial germination to sporocarp non-opening of mulberry sclerotiniose pathogenic bacteria by observing the potted pathogenic bacteria development process under a greenhouse mulberry garden tree, comprehensively and uniformly spraying methyl sulfur bacteria liquid on the ground of the mulberry garden, covering the ground with black fluffy ground cloth after liquid spraying ends, fixing joints through stainless steel rivets, and preventing ascospores from being spread; secondly, spraying methyl sulfur bacteria liquid again on the ground of the mulberry garden 5-7 days after liquid spraying especially in gaps of the ground cloth. The method has the advantages that the sclerotium base number in soil can be effectively reduced, apothecium is prevented from germinating from sclerotium, sclerotium initially germinating on the soil surface layer and non-opening sporocarp can be killed through ground disinfecting, ascospore is prevented from being spread after the ground is covered with the ground cloth, external pathogenic bacteria invasion is avoided through a steel-frame greenhouse, and infection sources are effectively controlled.

The invention belongs to the technical field of microbe culture and relates to a culture method of a cordyceps sinensis anamorphic hirsutella sinensis pure strain. The culture method comprises the following steps: A sampling: acquiring a wild cordyceps sinensis stroma of which ascospore is not ejected out, and storing; B separating: taking a strain in the cordyceps sinensis stroma, and inoculating into a flat plate under a sterile condition; C culturing, performing constant-temperature culture of the flat plate containing the strain in a culture box to obtain a hirsutella sinensis bacterial colony; D purifying, transferring the bacterial colony in the step C to a new flat plate for culture to obtain the hirsutella sinensis pure strain. By adoption of the culture method, the hirsutella sinensis pure strain can be obtained more easily, and the culture method is suitable for separating the cordyceps sinensis strain.

The invention belongs to the field of biotechnology, and provides a method for separating pathogenic fungi, in order to facilitate future research. Single spore isolation is the most effective method for separating parasitic pathogenic fungi, particularly suitable for the separation of pathogenic fungi, which are seriously polluted and difficult to separate by a traditional tissue isolation method, such as Venturia nashicola, apple Marssonina coronaria and Cercospora fungus. The traditional single spore isolation technique has complex operation, high technical requirements, and limited application. Employing capillary with diameter of 1 mm for drilling greatly simplifies the operation process, realizes strong operability of single spore isolation and higher speed; and general technical personnel can grasp the technology through simple training, so as to expand the scope of application. The method is mainly used for the separation of parasitic fungi and ascospores and gains good effect.

The invention provides a collection and storage method of cordyceps militaris ascospores. The collection method comprises the following steps: adding sand into a multi-spore separating and collecting container which can be sealed; sterilizing and drying; putting the selected matured sporocarp of cordyceps militaris ascocarps into the multi-spore separating and collecting container containing the sand; vibrating the multi-spore separating and collecting container so as to collect the sand containing matured ascospores which are ejected out or not; sealing the collected sand in a dry and sterile container; and preserving the container at room temperature by keeping out of the sun or in a refrigerator in a cold storage manner. According to the collection method, the operation is simple and the ascospore collecting rate is increased; the preserved ascospores can retain the inheritable characters of the original parents of cordyceps militaris after being activated and novel breeds for stress resistance can be screened.

The present invention relates to a system and method of cultivating Cordyceps, specifically, provided herein is a system of cultivating Cordyceps comprising: a Hepialus moth trapping device; a Hepialus moth eggs screening device, wherein the device is connected with the Hepialus moth trapping device; an ascospores-collecting device; a mycelia-preparing device; a conidia-preparing device; an infection device, wherein the device is connected with the ascospores-collecting device, the mycelia-preparing device and the conidia-preparing device; and a feeding device, wherein the device is connected with the Hepialus moth eggs screening device and the infection device. And also provided is a method of using the system to cultivate Cordyceps.

The invention belongs to the technical field of microbes, and particularly relates to a method for separating ceratocystis spp from soil. According to the method, a carrot is used for concentrating the ceratocystis spp, the ceratocystis spp is separated and purified by means of selecting a single ascospore group for lining. Due to the fact that part of operations does not require a strict aseptic condition, the method is applicable to bacterial strain collection during field studying and field investigation, and working efficiency is increased; by means of selecting the single ascospore group for lining, a target bacterium is separated and purified, separating and purifying efficiency is increased effectively, and time is saved; the method for separating the ceratocystis spp from the soil is low in cost, wide in utilization range, safe and environmental-friendly.

The invention relates to a method for breeding cordyceps militaris by hybridizing with ascospore on the basis of a molecular biological technology. The method specifically comprises the following steps: inducing and isolating cordyceps militaris monospores in a sterile environment and culturing to form colonies; using a PCR technology for detecting mating type genes MAT1-1-1, MAT1-1-2 and MAT1-2-1 of the colonies formed by the isolated monospores; selecting the colonies formed by the monospores containing the mating type genes MAT1-1-1 and MAT1-1-2 but not containing MAT1-2-1 and the colonies formed by the monospores containing the mating type gene MAT1-2-1 but not containing MAT1-1-1 and MAT1-1-2; respectively taking mycelia appropriate in size to be placed in a culture solution for dark culturing; inoculating an appropriate concentration of combination-cultured bacteria solution into an artificial culture medium of cordyceps militaris; culturing for a period of time to grow a new variety of cordyceps militaris sporophore from the artificial culture medium. The new variety of cordyceps militaris cultured by the method is easy to obtain relatively good yield and content of active substances of sporophores; a combination capable of stably producing high-quality cordyceps militaris sporophores can be obtained by counting the yield of sporophores and active substances cultured by different combinations; the method is relatively short in cycle and relatively small in workload, and is an efficient method for breeding cordyceps militaris.

The invention provides a method of preparing a novel beer-making yeast through a genome recombination technology and the obtained novel beer-making yeast, and belongs to the technical field of genomerecombination technologies. According to the method, yeast strains with good ethanol tolerance can be prepared. The method comprises the steps of adopting beer-making yeast and alcohol-resistant low-yield acetaldehyde yeast strains as original strains, and inducing the beer-making yeast and alcohol-resistant low-yield acetaldehyde yeast strains separately by utilizing an optimized spore productioncondition to form ascospores; utilizing a lywallzyme to release haploid spores in the ascospores of the beer-making yeast and alcohol-resistant low-yield acetaldehyde yeast strains, collecting haploid spore suspension liquids, and separating the haploid spores from respective haploid spore suspension liquids; fusing two haploid spores obtained after separation, placing the haploid spores into a wort culture medium in which the ethanol concentration is 12% to conduct culture, and obtaining the novel beer-making yeast (Saccharomyces cerevisiae) SCHL1706, wherein the preservation number is CGMCCNO.14231. The method of preparing the novel beer-making yeast through the genome recombination technology and the obtained novel beer-making yeast can be applied to preparation of the yeast strains with good ethanol tolerance and beer.

The invention provides a conidia coating method for producing a large number of rice blast germ sexual generations. The conidia coating method comprises the following steps: 1) preparing conidia suspensions of different mating types of rice blast germs; 2) coating conidium suspensions of different mating types on the oat culture medium according to a ratio of 1: 1; and 3) culturing for 5-15 days under the culture conditions that the culture temperature is 12-27 DEG C and the illumination is 5000lux-15000lux, so as to obtain a large number of rice blast fungus cocysts and ascospores. According to the method, the defects that sexual generation ascocysts and ascospores generated by a confrontation culture method in the prior art are small in total amount, unstable, large in sample difference, inconsistent in sexual offspring maturity and the like are overcome, a large number of rice blast bacteria sexual generations can be obtained within a short time, the formation speed of the ascocysts and the ascospores is high, the maturity is consistent, and the obtained ascocysts and ascospores can be used for quantitative comparison of sexual reproductive capacity of strains, sexual structure observation, nucleic acid extraction and gene expression quantitative analysis.

The invention relates to a genetic engineering strain producing lipase and a construction method thereof. The invention aims to solve the problem that as most of the existing lipase is imported and is expensive, biodiesel is more expensive. The cell of the genetic engineering strain producing lipase is elliptic and adopts budding reproduction to form ascospores; the spores are shaped like short mallets; the bacterial colony is circular, milky and non-transparent, and the surface is smooth and wet; and the enzyme activity of the produced lipase is high. The method comprises the following stepsof: extracting the DNAs (deoxyribonucleic acid) of the lipase-producing strain yarrowia lipolytica HDY-76 genome; performing polymerase chain reaction (PCR) amplification of the lip7; constructing the recombinant expression vector pGAPH alpha M-lip7; and constructing the genetic engineering strain producing lipase. The enzyme activity of the lipase produced by the constructed genetic engineering strain producing lipase is high. According to the invention, a foundation is laid for the application that the strain is used to catalyze oil through enzymolysis and produce biodiesel, thus the production cost of biodiesel can be reduced.

The invention discloses a cordyceps sinensis ascospore separating method. The method is characterized in that fresh ascospores popped by cordyceps sinensis are collected, after being diluted, sterilewater coats a germination culture medium, a common optical microscope is used for observing the germination condition of ascospores, under the dark environment, bright light spots formed by a light path of a microscope are used for marking the positions where ascospores are germinated in real time, a customized annular sampling device is used for being aligned to the light spot position for punching and sampling, the germinated monospores are transferred to an isolation medium for culture, and purebred ophiocordyceps sinensis obtained by developing single ascopspores is obtained. The method has the advantages of being simple, precise, high in efficiency, low in cost, capable of completing one separation operation every 90 s with the success rate being 70% or above, capable of being used for separation of large batches of cordyceps sinensis ascospores to obtain a large amount of purebred ophiocordyceps sinensis.

The invention provides a hypha coating method for massively producing rice blast germ sexual generations. The hypha coating method comprises the following steps: (1) preparing hypha suspensions of different mating types of rice blast germs; (2) coating hypha suspension liquid with different mating types on the oat culture medium according to a proportion; and 3) culturing for 5-15 days at the culture temperature of 12-27 DEG C to obtain a large number of rice blast fungus cocysts and ascospores. According to the method, the defects that sexual generation ascocysts and ascospores generated by a confrontation culture method in the prior art are small in total amount, unstable, large in sample difference, inconsistent in sexual offspring maturity and the like are overcome, a large number of rice blast bacteria sexual generations can be obtained within a short time, the formation speed of the ascocysts and the ascospores is high, and the maturity is consistent. The obtained ascocysts and ascospores can be used for quantitative comparison of sexual reproductive capacity of strains, sexual structure observation, nucleic acid extraction and gene expression quantitative analysis.

A method of treating an apple orchard to reduce infection of the apple orchard by Venturia inaequalis by administering EARTHTEC® or a derivative of EARTHTEC®. A method of reducing sporulation of V. inaequalis by administering EARTHTEC® or a derivative of EARTHTEC®. A method of reducing the dispersal of ascospores from V. inaequalis-infected apple debris in orchards by administering EARTHTEC® or a derivative of EARTHTEC®.

The present invention provides a Cordyceps militaris bacteria culturing method, which comprises the following steps of using ascospores formed by conidiospores of Cordyceps militaris in the asexual stage and / or fruit bodies of the Cordyceps militaris in the sexual stage as Cordyceps militaris multispores to be cultivated, performing shake culture of the multispores by adopting the PDA liquid culture medium with the culture temperature of 16-25 DEG C, the rotating speed of 100-140 rpm and the light-avoiding culture time of 120-240 hours so as to obtain multispore mycelium pellets, crushing themultispore mycelium pellets for 5-20 minutes with the rotating speed of 1000-20000 rpm, accessing the crushed bacterium to the static liquid culture medium for cultivation for 20-45 days, and obtaining excellent strains by tissue isolation mode when fruit bodies appear on the surface of the liquid culture medium. The method of the present invention enables to obtain excellent recombined strains in the shortest amount of time, and has the advantages of short period, simple operation, high probability of obtaining new combinations with best production performance by recombination, etc.

The invention relates to the technical field of cordyceps sinensis cultivation, in particular to a cordyceps sinensis ascospore collection device and a using method thereof. The device comprises a boxbody, a bottom plate, a top cover, windows, a collection device body, a fan, a rotating shaft, a temperature and humidity monitor and a light source. The cordyceps sinensis ascospore collection device can be used in the wild or a laboratory. The ascospore collection device can be not only used in the laboratory but also used in the wild for collecting ascospores of cordyceps sinensis, the collected ascospores are very wild ascospores, and therefore the quality and yield of the cordyceps sinensis can be further ensured; when the device is used in the laboratory, the ejection conditions of theasci can be controlled very well, and the quantity and quality of the collected ascospores are ensured; the ascospore collection device is simple in structure and convenient to operate, the cost can be reduced, and the labor can be saved.

The invention provides a culture medium capable of inducing mango gummosis bacteria to generate ascospores. The culture medium is obtained by adding aseptic pine needles on the basis of a PDA culture medium. Mango gummosis bacteria hyphe blocks are inoculated to the culture medium, cultured for 1 week in the present of light at 25-28 DEG C and then transferred to a sill in a culture chamber at 25-28 DEG C under sunlight irradiation to continue to be cultured for 1-2 weeks, so that plenty of perithecia, asci and ascospores can be generated. The simple, economical and effective culture medium and a method are provided for obtaining plenty of mango gummosis bacteria ascospores through artificial culture, and technical support is provided for on biological characteristics, infection characteristics and other genetic research of mango gummosis pathogenic bacteria.

The invention provides a method for separating and preserving Cordyceps militaris spawn. The method is that pure ascospores are separated and obtained from ripe ascocarp, and the ascospores are mixed with collection sand and are preserved. The target of Cordyceps militaris to be preserved is ascospores. Different from the traditional preserved fungal cells or conidiospores, sexual spores are preserved in the invention and can reserve the genetic characteristics of the parent to the maximum extent, so spawn degeneration is avoided. Moreover, when in separating the ascospores, the pure ascospores are obtained through the natural ascocarp ejection method, so the damages caused to the spores by the traditional washing method or scraping method are avoided and the activity of the spores is improved.

The invention discloses a truffle microcapsule and a preparation method and an application thereof, which belong to the technical field of preservation and processing of wild edible fungi. Truffle slurry obtained by crushing mature truffle is used as a core material and sodium alginate and chitosan are used as wall materials to prepare a microcapsule, and freeze drying is carried out on the microcapsule to obtain the truffle microcapsule. By adopting the method disclosed by the invention, such important biological active components as alpha-androstanol in the truffle are avoided from being oxidized, the loss of volatile aroma components is small, the effective components of the truffle can be furthest reserved, the storage period is long, and the truffle is conveniently transported and used. Two types of truffle core materials are obtained by adjusting the gap between a stator and a rotor of a colloid mill, the prepared microcapsule has two completely different usages, hyphae cells with large particle sizes contain integral ascospores and still have activity, so that the hyphae cells can be used as indoor or outdoor inoculants of mycorrhizal seedlings; spores of the hyphae cells with small particle sizes are broken, so that the nutritional ingredients are more beneficial for human body adsorption, and the truffle microcapsule is mainly used as a food additive and used for making truffle paste, truffle soup, seasoner, etc.

The invention discloses eurotium amstelodami and an application thereof. The eurotium amstelodami has the morphological characteristics that the eurotium amstelodami grows slowly on a CA culture medium, the diameter of a colony is 27-30 mm in 12-14 days at the temperature of 25 DEG C, the surface of the colony is flat, conidiospore structures and cleistothecium are mixed together, cleistothecium has a color of bright yellow, and the back side of the colony is yellow; the colony grows faster on a 20% cane sugar Czapek agar culture medium, and the diameter reaches 65-70 mm in about 10 days at the temperature of 25 DEG C; the colony is flat and thin or has irregular wrinkles, and a large amount of cleistothecium is generated and has a color of bright yellow; fewer conidiospores are generated; the back side of the colony is yellow; the cleistothecium is spherical or nearly spherical, the diameter is 80-170 mu m, the cleistothecium is exposed and has a color of bright yellow, asci are nearly spherical and have the diameter of 9-12 mu m, spores of asci are in a shape of a biconvex mirror and have the size of (4.5-5.5) mu m * (3.6-4.5) mu m, channels and two irregular ridges are formed between two parts, and the asci are not flat and are rough; conidial heads are in a radial shape, acrocyst is in a shape of a flask, the diameter is 16-25 mu m, the sporogenous structure adopts a single layer, the phialide is (5-7) mu m * (3.2-3.5) mu m, the conidiospores are spherical or nearly spherical and have the diameter of 4-6 mu m, a minority of the conidiospores are oval and have the diameter of (5-6.5) mu m * (3-5) mu m, and thornlet grows on the wall. The eurotium amstelodami can promote generation of flowery and fruity substances with liquor body flavor.

The invention belongs to the technical field of biology and relates to bacteriostatic protease CmYC1 prepared through eukaryotic fermentation and application of the bacteriostatic protease CmYC1. Thenovel bacteriostatic protease CmYC1 can be used for inhibiting the growth of sclerotinia sclerotiorum, botrytis cinerea, sclerotinia minor, mucor hiemails, rhizoctonia solani, fusarium graminearum, fusarium oxysporum and pythium aphanidermatum, inhibit the infection of the ascospores of the sclerotinia sclerotiorum on leaves of oilseed rapes and has a great potential of being developed into a bacteriostatic agent.