10 results about "Ascospore" patented technology

The invention belongs to the technical field of cordyceps sinensis cultivation, and particularly relates to an ascospore collecting device for cultivating cordyceps sinensis and a using method thereof.The ascospore collecting device comprises a collecting box, a cover plate is rotationally installed on the front side of the collecting box, a collecting plate is movably inserted into the lower end of the collecting box, and multiple sets of supporting rods are fixedly installed in the collecting box; a containing table is fixedly installed among the multiple sets of supporting rods, the containing table is lower than the lower end of the cover plate, a driving box is fixedly installed at the upper end of the collecting box, a first gear is rotationally installed in the driving box, and fan blades are fixedly installed at the lower end of the first gear. Through the structural cooperation, light energy can be converted into electric energy in an illumination environment, so that the electric energy is provided for each element in the collection box, an external power supply is not needed, the use convenience of the device is improved, meanwhile, through the arrangement of the control assembly, the angle of the photovoltaic assembly can be conveniently adjusted, and the use convenience is improved. Therefore, the angle of the photovoltaic module can be adjusted according to the irradiation angle of the sun.

The present invention relates to Saccharomyces cerevisiae yeast ascospores in dry or suspension form, for use as a medicament, and in particular for use as an anti-inflammatory agent, antiviral agent or anti-tumour agent. The present invention also relates to Saccharomyces cerevisiae yeast ascospores characterized in that they are in dry form and in that they have a solids content ranging from 95% to 99.9%. The invention further relates to a method for preparing the Saccharomyces cerevisiae yeast ascospores in dry or suspension form. The method for preparing the ascospores in dry form consists in subjecting to drying a suspension of ascospores having a solids content ranging from 1.5% to 5%, said drying being carried out using a fluidized bed or by freeze-drying.

The application specifically relates to a method for preparing glutathione by immobilized enzyme and application. The method for preparing glutathione provided by the application comprises synthesizing glutathione by using saccharomyces cerevisiae haploid ascospores as a fixed enzyme catalyst to catalyze substrate reaction, using an ATP in-situ regeneration reaction system of phosphoenolpyruvate as an energy supply system, the saccharomyces cerevisiae haploid ascospores are knocked out or inactivated in combination of one or more than two of cell wall beta-glucan synthesis genes, and GSH1 genes, GSH2 genes and PYK1 genes are co-expressed. The saccharomyces cerevisiae haploid ascospores for simultaneously producing GSH1, GSH2 and pyruvate kinase are used as a fixed enzyme catalyst to catalyze synthesis of glutathione, three enzymes no longer need to be added externally, raw material cost is greatly reduced, the obtained product is easy to purify, and the prepared glutathione is high in purity.

The invention relates to a method for separating and purifying ascospores of cordyceps sinensis strains, and belongs to the field of ascospore strain separation and culture. The method comprises the following steps: firstly, removing impurities such as bacteria and fungi through gradient forward flushing and efficiently recovering target ascospores through reverse flushing by virtue of a three-stage gradient volume flushing, filtering and purifying process; performing primary culture and isolated culture on the filtered and purified ascospores, adding chloramphenicol into a culture medium to inhibit bacterial pollution, and simulating an ascospore primary environment to perform constant-temperature and constant-humidity culture; and after the bacterial colony is visible to the naked eyes, transferring and germinating the monospores into the culture medium by adopting a metal agar gel puncher, and continuously culturing to obtain the pure cordyceps sinensis mycelia developed from the single ascospores. The invention provides a separation and purification method for efficiently, purely and controllably separating the cordyceps sinensis fungus ascospores, effectively breaking the dormancy of the cordyceps sinensis fungus ascospores and remarkably improving the germination rate, and a high-purity and active cordyceps sinensis fungus pure culture can be obtained.

The invention discloses an isolated culture method of cordyceps sinensis fungi, and relates to the related technical field of cordyceps sinensis fungi. According to the method, the germination rate of the ascospores is increased, the germination rate of the ascospores reaches 20%-50% through an improved purification and culture method, the germination rate of the spores is increased compared with a traditional method, and growth of subsequent hyphae is ensured; by using the 1: 10 soil leachate and combining specific temperature control (15-20 DEG C), the growth of cordyceps sinensis hyphae is effectively promoted, the colonial morphology conforms to the natural growth characteristic, and the stability is high; according to the method, the culture period is greatly shortened, the growth speed of hyphae is obviously increased through an improved culture medium formula and environment control, bacterial colonies can reach 2-6 mm within 2 months, bacterial colonies with the diameter reaching 1-2 cm can be formed within 3-4 months, the culture period is shortened, and the yield is increased.

A method for making a chitosan product from yeast cells is disclosed herein. Yeast cells are cultured to form a biomass of yeast cells. The yeast cells are induced to undergo meiosis causing the yeast cells to form asci containing ascospores wherein each ascospore contains a chitosan protective layer in the ascospore wall. Chitosan is extracted from the ascospores, purified to form purified chitosan, and precipitated and dried to form a chitosan product.

The invention discloses a wheat scab inducing hotbed device, and belongs to the technical field of wheat scab inducing hotbed devices, the wheat scab inducing hotbed device comprises a box body, a box cover arranged above the box body and a seedling raising box arranged in the box body, the box cover is internally provided with a first cavity and two piston grooves communicated with the first cavity, and the piston grooves are communicated with the first cavity. And first electric telescopic rods are fixedly connected to the inner walls of the two piston grooves. According to the device, when a first fan works, air in the box body circulates internally, ascospores in the induction process in the box body can be mature and scattered on wheat anther, and when a second fan in an air inlet pipe and an air outlet pipe works, external air can be brought into the box body, so that the wheat anther can be effectively induced. Fungi in the box body are prevented from overflowing under the action of a TPU waterproof breathable film, the first electric telescopic rod enables the fungi in the air flowing through the antifungal solution in the box body to be killed, and the influence on the respiratory tract or skin of an experimenter caused by overflowing of fusarium in the induction process is relieved.

The invention relates to a type-a haploid saccharomyces cerevisiae and a screening method and application thereof, the type-a haploid saccharomyces cerevisiae is saccharomyces cerevisiae JNU2024, and has been preserved in Guangdong Microbial Culture Collection Center on February 20, 2023, the preservation number is GDMCC No: 65932, and the preservation address is building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou. The screening method of the type a haploid saccharomyces cerevisiae comprises the following steps: S1, coating a spore production culture medium with diploid saccharomyces cerevisiae thalli for culturing to obtain a first mixture containing saccharomyces cerevisiae ascospores and yeast vegetative cells; s2, treating the first mixture with helicase liquid to digest ascocyst walls of the saccharomyces cerevisiae ascospores to obtain a second mixture containing haploid ascospores and yeast vegetative cells; s3, performing water bath heating treatment on the second mixture, and killing the yeast vegetative cells to obtain haploid ascospores; s4, coating a YPD culture medium with the haploid ascospores, and culturing to obtain haploid saccharomyces cerevisiae; s5, identifying the type of the haploid saccharomyces cerevisiae obtained in S4 by using PCR (Polymerase Chain Reaction); and S6, taking the type a haploid saccharomyces cerevisiae obtained by screening and identification in the step S5 as a starting strain, carrying out ARTP mutagenesis, and screening to obtain the saccharomyces cerevisiae JNU2024.

The invention relates to the technical field of ganoderma lucidum fermentation technology, and particularly discloses a ganoderma lucidum fermentation technology of Aspergillus cristatus, comprising: S1, transferring a bacterial block to a 5% NaCl+MYA solid culture medium in a clean bench, culturing at a constant temperature and humidity of 28 DEG C for 7 days, and repeating this process twice to activate the strain; inoculating the activated bacterial strain onto a 5% NaCl+MYA plate culture medium, and culturing at a constant temperature and humidity of 28 DEG C for 7 days; adding an appropriate amount of sterile water to the Aspergillus cristatus plate cultured for 7 days, scraping spores, taking 1 mL of spore liquid and adding it to a centrifuge tube containing glass beads, shaking to break the ascospores, counting them with a hemocytometer, and diluting them into a spore suspension of corresponding concentration; S2, weighing an appropriate amount of ganoderma lucidum blocks into a fermentation bottle, adding distilled water, sterilizing at 121 DEG C for 20 minutes, cooling to room temperature, inoculating an appropriate amount of spore suspension, and culturing at a constant temperature and humidity of 28 DEG C for 10 days. The *Aspergillus cristatus*-fermented *Ganoderma lucidum* fruiting bodies obtained by this invention exhibit high bioactivity, and the bitterness of *Ganoderma lucidum* is significantly improved.

This invention belongs to the field of microbial culture technology, specifically relating to a culture medium formulation and corresponding culture method for efficiently inducing Cannomonas to produce perithecia. This invention provides a method for inducing Cannomonas (… Monosporascus cannonballus The culture medium for producing ascocarps: Add water to a mixture of carbon source, nitrogen source, inorganic salts, and plant tissue to a final volume of 1 L, adjust the pH to 7±0.1, add 20.0 g of agar powder, and then sterilize. This invention also provides a method for inducing ascocarp production in *Cannomonas*: Inoculate *Cannomonas* onto a culture medium plate, place the inoculated plate at 32±0.5℃, and culture using a light-dark cycle of 16 hours of light and 8 hours of darkness per day for 14-18 days. The method of this invention has technical advantages such as high sporulation efficiency and a short cycle.