5 results about "Ascospore" patented technology

The present invention relates to Saccharomyces cerevisiae yeast ascospores in dry or suspension form, for use as a medicament, and in particular for use as an anti-inflammatory agent, antiviral agent or anti-tumour agent. The present invention also relates to Saccharomyces cerevisiae yeast ascospores characterized in that they are in dry form and in that they have a solids content ranging from 95% to 99.9%. The invention further relates to a method for preparing the Saccharomyces cerevisiae yeast ascospores in dry or suspension form. The method for preparing the ascospores in dry form consists in subjecting to drying a suspension of ascospores having a solids content ranging from 1.5% to 5%, said drying being carried out using a fluidized bed or by freeze-drying.

The application specifically relates to a method for preparing glutathione by immobilized enzyme and application. The method for preparing glutathione provided by the application comprises synthesizing glutathione by using saccharomyces cerevisiae haploid ascospores as a fixed enzyme catalyst to catalyze substrate reaction, using an ATP in-situ regeneration reaction system of phosphoenolpyruvate as an energy supply system, the saccharomyces cerevisiae haploid ascospores are knocked out or inactivated in combination of one or more than two of cell wall beta-glucan synthesis genes, and GSH1 genes, GSH2 genes and PYK1 genes are co-expressed. The saccharomyces cerevisiae haploid ascospores for simultaneously producing GSH1, GSH2 and pyruvate kinase are used as a fixed enzyme catalyst to catalyze synthesis of glutathione, three enzymes no longer need to be added externally, raw material cost is greatly reduced, the obtained product is easy to purify, and the prepared glutathione is high in purity.

The invention relates to a method for separating and purifying ascospores of cordyceps sinensis strains, and belongs to the field of ascospore strain separation and culture. The method comprises the following steps: firstly, removing impurities such as bacteria and fungi through gradient forward flushing and efficiently recovering target ascospores through reverse flushing by virtue of a three-stage gradient volume flushing, filtering and purifying process; performing primary culture and isolated culture on the filtered and purified ascospores, adding chloramphenicol into a culture medium to inhibit bacterial pollution, and simulating an ascospore primary environment to perform constant-temperature and constant-humidity culture; and after the bacterial colony is visible to the naked eyes, transferring and germinating the monospores into the culture medium by adopting a metal agar gel puncher, and continuously culturing to obtain the pure cordyceps sinensis mycelia developed from the single ascospores. The invention provides a separation and purification method for efficiently, purely and controllably separating the cordyceps sinensis fungus ascospores, effectively breaking the dormancy of the cordyceps sinensis fungus ascospores and remarkably improving the germination rate, and a high-purity and active cordyceps sinensis fungus pure culture can be obtained.

A method for making a chitosan product from yeast cells is disclosed herein. Yeast cells are cultured to form a biomass of yeast cells. The yeast cells are induced to undergo meiosis causing the yeast cells to form asci containing ascospores wherein each ascospore contains a chitosan protective layer in the ascospore wall. Chitosan is extracted from the ascospores, purified to form purified chitosan, and precipitated and dried to form a chitosan product.

This invention belongs to the field of microbial culture technology, specifically relating to a culture medium formulation and corresponding culture method for efficiently inducing Cannomonas to produce perithecia. This invention provides a method for inducing Cannomonas (… Monosporascus cannonballus The culture medium for producing ascocarps: Add water to a mixture of carbon source, nitrogen source, inorganic salts, and plant tissue to a final volume of 1 L, adjust the pH to 7±0.1, add 20.0 g of agar powder, and then sterilize. This invention also provides a method for inducing ascocarp production in *Cannomonas*: Inoculate *Cannomonas* onto a culture medium plate, place the inoculated plate at 32±0.5℃, and culture using a light-dark cycle of 16 hours of light and 8 hours of darkness per day for 14-18 days. The method of this invention has technical advantages such as high sporulation efficiency and a short cycle.