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Cordyceps militaris bacteria culturing method

A cultivation method and technology of Cordyceps militaris are applied in the directions of botanical equipment and methods, fertilizer mixtures, gardening, etc., to achieve the effects of improving adaptability, simple operation and short cycle.

Inactive Publication Date: 2012-08-15
BEIJING AGRO BIOTECH RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is possible to breed both high-yield genes and poor-quality combinations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Experimental strain: the Cordyceps militaris strain (purchased from the Institute of Microbiology, Chinese Academy of Sciences) was used.

[0040] (1) Source and acquisition method of polyspores:

[0041] Using the newly isolated Cordyceps militaris slant plate, under the light intensity of more than 300 lux, the temperature was kept at 23°C, and the irradiation time was based on the collapse of the aerial hyphae on the surface and the formation of a large number of conidia. Then wash with sterile water, and collect them in a sterilized container to obtain mixed conidia.

[0042] (2) Multi-spore shake flask proliferation culture:

[0043] The polyspore isolate obtained in the above steps was multiplied and cultured in a Erlenmeyer flask with PDA liquid medium at a culture temperature of 22° C. and a rotational speed of 140 rpm, and cultured in the dark. After 120 hours of shaking culture, the mycelium balls are filled with the whole liquid medium, and the culture is s...

Embodiment 2

[0053] Experimental strain: the Cordyceps militaris strain (purchased from the Institute of Microbiology, Chinese Academy of Sciences) was used.

[0054] (1) Source and acquisition method of polyspores:

[0055] Ascospores formed by the fruiting body of Cordyceps militaris in the sexual stage: select the mature fruiting body and cut the rich part of the ascocarp with scissors through aseptic operation, then put it into a sterilized petri dish, collect about 5-10 pieces, and use The pin fixes it on the tampon of the sterilized Erlenmeyer flask, and finally the tampon that has fixed the fruiting bodies is still inserted into the Erlenmeyer flask. Place it at room temperature for 10-15 days, and shoot the mature ascospores in the empty triangular flask.

[0056] (2) Multi-spore shake flask proliferation culture:

[0057] The ascospores obtained in the above steps were multiplied and cultured in a Erlenmeyer flask with PDA liquid medium at a culture temperature of 16° C. and a r...

Embodiment 3

[0067] Experimental strain: the Cordyceps militaris strain (purchased from the Institute of Microbiology, Chinese Academy of Sciences) was used.

[0068] (1) Source and acquisition method of polyspores:

[0069] Using the newly isolated Cordyceps militaris slant plate, under the light intensity of more than 300 lux, the temperature was kept at 25°C, and the irradiation time was based on the collapse of the aerial hyphae on the surface and the formation of a large number of conidia. Then wash with sterile water, and collect them in a sterilized container to obtain mixed conidia.

[0070] (2) Multi-spore shake flask proliferation culture:

[0071] The polyspore isolate obtained in the above steps was multiplied and cultured in a Erlenmeyer flask with PDA liquid medium at a culture temperature of 25° C. and a rotational speed of 120 rpm, and cultured in the dark. After 160 hours of shaking culture, the mycelium balls are filled with the whole liquid medium, and the culture is s...

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Abstract

The present invention provides a Cordyceps militaris bacteria culturing method, which comprises the following steps of using ascospores formed by conidiospores of Cordyceps militaris in the asexual stage and / or fruit bodies of the Cordyceps militaris in the sexual stage as Cordyceps militaris multispores to be cultivated, performing shake culture of the multispores by adopting the PDA liquid culture medium with the culture temperature of 16-25 DEG C, the rotating speed of 100-140 rpm and the light-avoiding culture time of 120-240 hours so as to obtain multispore mycelium pellets, crushing themultispore mycelium pellets for 5-20 minutes with the rotating speed of 1000-20000 rpm, accessing the crushed bacterium to the static liquid culture medium for cultivation for 20-45 days, and obtaining excellent strains by tissue isolation mode when fruit bodies appear on the surface of the liquid culture medium. The method of the present invention enables to obtain excellent recombined strains in the shortest amount of time, and has the advantages of short period, simple operation, high probability of obtaining new combinations with best production performance by recombination, etc.

Description

technical field [0001] The invention belongs to the technical field of Cordyceps militaris cultivation, and relates to a method for screening and rejuvenating Cordyceps militaris strains. Background technique [0002] Cordyceps militaris (L.ex Fr) Link, also known as Cordyceps militaris and Cordyceps militaris, parasitizes on the pupal bodies of insects such as Lepidoptera, Coleoptera, and Diptera. It grows in low-altitude plains and is mainly distributed in Northeast, North China, and Northwest China. Modern research shows that Cordyceps militaris contains cordycepin (3'-deoxyadenosine), cordycepic acid (D-mannitol), adenosine, cordyceps polysaccharide, ergosterol, superoxide dismutase (SOD), selenium (Se), etc. chemical composition. It has the effects of improving human immune function, anti-cancer, anti-bacterial, anti-fatigue, anti-aging, anti-convulsions, hypoxia resistance, sedation, aphrodisiac and kidney strengthening. At the same time, it also has the effects of ...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00
Inventor 邢礼军苏炳豪马荣才苏坚宏王旭明
Owner BEIJING AGRO BIOTECH RES CENT
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