Rhodotorulasp.2-14 and method for producing trehalose using same

A technology of red yeast and trehalose, applied in the field of microorganisms, can solve the problems of low yield and high extraction cost, and achieve the effects of high yield, low cost, and easy popularization and application.

Inactive Publication Date: 2011-08-17
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] Another technical problem to be solved by the present invention is to provide a method for producing trehalose by the above-mentioned Rhodotorula strain, so as to overcome the problems of low yield and high extraction cost in the prior art

Method used

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  • Rhodotorulasp.2-14 and method for producing trehalose using same
  • Rhodotorulasp.2-14 and method for producing trehalose using same
  • Rhodotorulasp.2-14 and method for producing trehalose using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1. A red yeast 2-14 ( Rhodotorula sp. . 2-14) CGMCC NO. 4445. This strain has the following characteristics:

[0060] (1) Morphological characteristics: the cells are round or oval in shape, the size is 4.5~5.1×6.3~7.2μm, the mode of reproduction is budding, and no pseudohyphae, ascospores and spores are formed;

[0061] (2) Culture characteristics: the fermentation liquid is clear, the surface is free of mold, rings, bubbles, and sediment; the colonies are plump and moist, red in color, neat in edge, free of wrinkles, and aerobic;

[0062] (3) Physiological and biochemical characteristics: the strain is resistant to lactose, galactose, soluble starch, L-sorbose, D-xylose, D-fructose, L-rhamnose, maltose, trehalose, mannitol, ribose, glucose, sucrose , succinic acid, and citric acid were not fermented; the strain did not liquefy gelatin, was positive for urease, negative for glucose oxidation, positive for DBB, negative for the formation of starch-like compou...

Embodiment 2

[0063] Example 2. A kind of Rhodotorula 2-14 as described in embodiment 1 ( Rhodotorula sp. .2-14) CGMCC NO. 4445 method for producing trehalose, the steps are as follows:

[0064] (1) Preparation of strains: Streak the strains of Rhodotorula 2-14 on a sterilized rich medium plate, culture it statically at 30°C for 2 days, pick a single colony and culture it on a slant of rich medium for 2 days, and then store it Store in a 4°C refrigerator for later use; the rich medium is: 20% potato, 2% sucrose, and 1.5% agar;

[0065] (2) Cell culture: pick the strains cultured on the slant, inoculate them into a 250mL Erlenmeyer flask filled with 50mL of liquid medium, and culture them on a shaker at 20°C and 100 r / min for 18 hours to obtain a seed solution. 5% of the inoculum was inoculated into a 250mL Erlenmeyer flask containing 75mL of liquid medium, cultured on a shaking table at 28°C at 160 r / min for 50 hours, and then removed by centrifugation at 5000 r / min to remove the supernat...

Embodiment 3

[0067] Example 3. A kind of Rhodotorula 2-14 as described in embodiment 1 ( Rhodotorula sp. .2-14) CGMCC NO. 4445 method for producing trehalose, the steps are as follows:

[0068] (1) Preparation of strains: Streak the strains of Rhodotorula 2-14 on a sterilized rich medium plate, culture it statically at 30°C for 2 days, pick a single colony and culture it on a slant of rich medium for 2 days, and then store it Store in a 4°C refrigerator for later use; the rich medium is: 20% potato, 2% sucrose, and 1.5% agar;

[0069] (2) Cell culture: Pick the strains cultured on the slant, inoculate them into a 250mL Erlenmeyer flask containing 50mL of liquid medium, and culture them on a shaker at 35°C and 200 r / min for 30 hours to obtain a seed solution. 5% of the inoculum was inoculated into a 250mL Erlenmeyer flask containing 75mL of liquid medium, cultured on a shaking table at 28°C at 160 r / min for 60 hours, and then removed by centrifugation at 5000 r / min to remove the supernata...

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Abstract

The invention provides a Rhodotorulasp.2-14 China general microbiological culture collection (CGMCC) NO. 4445. The Rhodotorulasp.2-14 is circular or oval, the size of the Rhodotorulasp.2-14 is 4.5-5.1*6.3-7.2mu m, and the reproduction mode is budding and is free of the formation of pseudohypha, ascospore and ballistospore. The invention further discloses a method for producing trehalose using the Rhodotorulasp.2-14. The trehalose of a bacterial strain is high in yield, simple in a culture medium and cultivation process, and energy-saving and environment-friendly in extraction process. The method for producing the trehalose has the advantages of being simple and practical, and low in cost, thereby being easy to popularize and apply in the industrial production.

Description

technical field [0001] The present invention relates to a microorganism, especially Rhodotorula 2-14 ( Rhodotorula sp. 2-14) CGMCC NO. 4445 (preserved in CGMCC, General Microorganism Center of China Microbiological Culture Collection Management Committee on December 8, 2010, and the preservation number is CGMCC No. 4445); the present invention also relates to the use of this bacterial strain to produce seaweed sugar method. Background technique [0002] Trehalose is composed of two molecules of glucopyranose monomers connected by α-1,1 glycosidic bonds, its chemical name is α-D-glucopyranosyl-α-D-glucopyranoside, and its molecular formula is C 12 h 22 o 11 2H 2 O, relative molecular weight is 378.33 (containing two molecules of crystal water), specific rotation [α] D =+178°(20°C), the melting point is 97°C, and it will lose crystal water when heated. Because of its symmetrical molecular structure and no hemiacetal group, it has neither reduction nor mutarotation. Its ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P19/12C12R1/645
CPCY02P20/10
Inventor 赵玉巧杜云建顾玲玲
Owner HUAIHAI INST OF TECH
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