Conidia coating method for producing large number of rice blast germ sexual generations
A technology of conidia and rice blast fungus, applied in the field of microorganisms, can solve the problems of large differences between samples, instability, and inability to support the sample size, and achieve the effect of consistent maturity and fast formation speed
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Embodiment 1
[0036] Embodiment 1, 4×10 4 Individual / mL Magnaporthe grisea spore suspension to prepare sexual generation
[0037] 1. Strains and culture conditions
[0038] Pyricularia oryzae strains Guy11 (MAT1-1), TH16 (MAT1-1), 2539 (MAT1-2), TH3 (MAT1-2) were cultured on complete medium (CM). Oatmealagar medium (OMA) was used to induce sexual generations of strains with different mating types.
[0039] CM Medium Formula (1L)
[0040]
[0041] The above reagents were weighed according to the corresponding amount and then adjusted to the corresponding volume. The pH was adjusted to 6.5 with NaOH, and 15 g of agar powder was added per liter of solid medium, and sterilized by high-pressure steam at 121 ° C for 15 min.
[0042] in:
[0043] 1000×Vitamin solution (100ml):
[0044]
[0045] The above reagents were dissolved in distilled water, and the volume was adjusted to 100ml, and stored in a refrigerator at 4°C.
[0046] 1000×Trace Elements (100ml):
[0047]
[0048] The a...
Embodiment 2
[0069] Embodiment 2, 5×10 3 Individual / mL Magnaporthe grisea spore suspension to prepare sexual generation
[0070] Select Guy11+TH3 for use, the blast fungus spore suspension in embodiment one is adjusted to 5 * 10 3 individual / mL, the illumination was 5000lux, and the sexual generation ( Figure 7 ).
Embodiment 3
[0071] Embodiment three, 5×10 3 Individual / mL Magnaporthe grisea spore suspension to prepare sexual generation
[0072] Select Guy11+TH3 for use, the blast fungus spore suspension in embodiment one is adjusted to 5 * 10 3 cells / mL, the light was 10000lux, and the sexual generation was obtained.
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