Bacillus velenzensis and application thereof to biological control

A technology for separating Bacillus leissi and Bacillus, applied to Bacillus veleis and its application in biocontrol, can solve problems such as yield loss of rapeseed black shank

Active Publication Date: 2020-06-09
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The species complex consisting of Lm and Lb is associated with the most devastating disease of oilseed rape (Brassica napus), and oilseed rape blackleg causes significant yield losses worldwide

Method used

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  • Bacillus velenzensis and application thereof to biological control
  • Bacillus velenzensis and application thereof to biological control
  • Bacillus velenzensis and application thereof to biological control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Screening and Identification of Bacillus Velez Bv-6:

[0038]The bacterial strain involved in the present invention is isolated from a rapeseed endophytic bacteria sample collected in Wuhan City, Hubei Province, and the bacterial strain Bv-6 has an obvious effect after antagonism testing and plant growth promoting testing. It was identified as Bacillus velezensis by morphology and molecular biology. The strain was sent to the China Center for Type Culture Collection on December 25, 2019. It was named after classification: Bacillus velezensis Bv-6 , the deposit number is: CCTCC NO: M 20191106 Address: Wuhan University, Wuhan, China.

[0039] Bacillus Velez belongs to Gram-positive bacteria. Its body is rod-shaped and its size is 0.5μm×(1.5~3.5)μm. Stress-resistant spores can be produced in the middle or at the tail, and the spores are oval. In three-section line culture of beef extract peptone (NA) medium, the single colony was milky white, with rough surface and irreg...

Embodiment 2

[0041] The preparation of the bacterial agent of Bacillus Velez Bv-6:

[0042] Fermentation broth preparation:

[0043] Streak inoculate the Bacillus velei Bv-6 preserved in glycerol at -80°C on the solid NA medium, culture it in a 30°C incubator for 24 hours, and then pick a single colony and inoculate it into 100mL of liquid NB culture In the medium, shake culture under the condition of 30 ℃ and 180rpm for 24h, as the 10L fermenter seed liquid of Bacillus veleisi Bv-6.

[0044] The formula of the fermentation medium is beef extract 3g / L, soybean peptone 5g / L, glucose 20g / L, pH 7.

[0045] Sterilize 6L of the above-mentioned fermentation medium in a 10L fermenter by high-pressure steam at 121°C for 0.5h, cool down to 40°C after sterilization, and immediately inoculate 1×10 7 300mL (5% inoculum size) of Bacillus velei seed solution at cfu / mL concentration, cultivated for 72 hours at a culture temperature of 30°C, a rotating speed of 300r / min, an air pressure of 0.05MPa, and ...

Embodiment 3

[0052] Determination of the Effect of Bacillus Velez Bv-6 on Leptosphaeria biglobosa (Lb) Infection on Rapeseed Leaves

[0053] Wash the conidia of the Lb strain with sterile water, put them in a 50mL centrifuge tube, filter through four layers of lens-cleaning paper to obtain a conidia suspension, and adjust its concentration to 1×10 8 spores / mL.

[0054] Preparation of powder suspension: get 3 g of the powder prepared in Example 2 and dilute it in 30 mL sterile water, and dilute to a concentration of bacteria equal to 1×10 7 cfu / mL to obtain a powder suspension.

[0055] On each true leaf of rapeseed (Zhongshuang No. 9), the Bacillus Veles Bv-6 fermentation liquid and powder suspension prepared in Example 2 are used to evenly smear on the surface (about 10 μ L of Bv-6 is added dropwise on each true leaf). fermentation broth, powder suspension). The leaves treated with NB solution were used as the control, and each treatment was repeated 4 times (the 4 repetitions here ref...

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Abstract

The invention belongs to the technical field of agricultural microorganisms, in particular to Bacillus velenzensis and application thereof to biological control. The Bacillus velenzensis disclosed bythe invention is Bacillus velezensis Bv-6, and a preservation number is CCTCC NO:M20191106. The Bacillus velenzensis fermented solutions or powder can effectively inhibit growth of mycelia of important pathogens (Sclerotinia sclerotiorum(Lib.)de Bary, Botrytis cinerea Pers. and Blackleg) on rapes, and germination of conidiophores and ascospores; and through seed soaking treatment of the fermentedsolutions, the seeds of the rapes can be normally germinated and grown, and the edisease resistance of plants can be improved. In addition, the Bacillus velenzensis has the characteristics of being high in proliferation speed, high in stability, high in temperature resistance and free of toxicity. Therefore, the Bacillus velezensis strain Bv-6 has the potential of developing and utilizing the function of the biological control.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a strain of Bacillus velesii and its application in biocontrol. Background technique [0002] Sclerotinia sclerotiorum (Lib.) de Bary] is an important soil-borne plant pathogen that causes disease in hundreds of plant species in 75 families worldwide, including rapeseed, soybean, cabbage and other important crops, leading to its yield reduced, reduced quality. Among these, rapeseed yield losses can be significant, ranging from 10% to 80% in China. Currently, fungicides and crop rotation are the traditional methods of controlling this pathogen. But fungicides are expensive and dangerous, and some fungicides, such as carbendazim and dimethicone, lose effectiveness as resistance develops in Sclerotinia populations. Moreover, long-term use of fungicides will lead to drug resistance of Sclerotinia sclerotiorum, fungicide and pesticide residues, damage to so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N65/08A01P3/00A01P21/00C12R1/07
CPCC12N1/20A01N65/00A01N65/08C12R2001/07C12N1/205
Inventor 李国庆孙力刘晨杨龙吴明德张静
Owner HUAZHONG AGRI UNIV
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