Culture medium capable of inducing mango gummosis bacteria to generate ascospores

A technology of ascospores and culture medium, which is applied in the field of media for inducing the production of ascospores with genital ascospores by mango glioma pathogen, can solve the difficulty of ascospores, hinder the research on prevention and control technology, and affect the life history research of mango glioma pathogenic bacteria, etc. problem, to achieve the effect of simple operation and low price

Inactive Publication Date: 2016-11-09
INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, its sexual ascus and ascospores have only been observed in a small amount on the host plants in the field, and there are no reports of the fungus producing ascus, ascus and ascospores on artificial medium at home and abroad.
Common media for fungi such as potato dextrose agar (PDA) and oat media can satisfy the mycelial growth of the pathogen, but can

Method used

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  • Culture medium capable of inducing mango gummosis bacteria to generate ascospores
  • Culture medium capable of inducing mango gummosis bacteria to generate ascospores
  • Culture medium capable of inducing mango gummosis bacteria to generate ascospores

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1 Effects of different culture media on the growth of mycelium and production of ascospores of mango cocoa dispora

[0035] 1. Formulas and production methods of seven culture media:

[0036] (1) Potato Dextrose Agar Medium (PDA)

[0037] Glucose 20g, peeled potatoes 200g, agar 20g, distilled water 1000mL, fresh pine needles. Add 200g of peeled potatoes, add 1000mL of water to boil for 15min, filter with four layers of gauze, then dilute the filtered juice to 1000mL, add 15-20g of agar powder, add the agar powder to melt, then subpackage and sterilize.

[0038] (2) Oat agar medium (OA)

[0039] Oatmeal 30g, agar 15-20g, water 1000mL. Add 1000mL of water to oatmeal, boil for 30min, add water to make up 1000mL after filtering through gauze, add agar powder to melt, subpackage and sterilize.

[0040] (3) Sterile pine needles

[0041] Cut the pine needles to a length of 5-8cm, weigh 1-3g, disinfect the surface with 1% sodium hypochlorite for 10 minutes, rinse w...

Embodiment 2

[0052] The influence of embodiment 2 different mediums on the growth of Thielavia mango Aesculus and the production of ascospores

[0053] Thielavia hyphae pieces (5-8mm in diameter) of Aesculus mangoesculus cultured on PDA for 4-5 days were inoculated into the seven mediums prepared in Example 1 respectively. Potato dextrose agar (PDA), oat medium (OA), sterile pine needles, cypress leaves, water agar plus pine needles, PDA medium containing sterile pine needles, potato dextrose agar plus cypress leaves, etc., placed at 25 °C Cultivate under light (400-1500LUX) at -28°C for 7 days, and then place it on the window sill of a 25°C-28°C cultivation room irradiated by sunlight for 7-14 days in a natural state. It was found that Thielavia aesculus grows slowly or does not grow on pine needles, cypress leaves, water agar plus pine needles, grows rapidly on PDA and OA mycelium but does not produce ascospores, and on the PDA medium containing aseptic pine needles, Mycelia grow rapidl...

Embodiment 3

[0054] The influence of embodiment 3 different mediums on the growth of Thielavia mango and the production of ascospores

[0055] Will be cultured on PDA 4-5 days Thielavia mangoe small new mycelia piece (diameter 5-8mm), inoculate to seven kinds of potato dextrose agar (PDA) that embodiment 1 makes respectively, oat culture medium (OA) , sterile pine needles, cypress leaves, water agar plus pine needles, PDA medium containing sterile pine needles, potato dextrose agar plus cypress leaves, etc., placed at 25°C-28°C under light (400-1500LUX) for 7 days , and then placed on the window sill of a 25°C-28°C cultivation room illuminated by sunlight to continue culturing for 7-14 days in a natural state. As a result, it was found that Thielavia small neoformans grew slowly or did not grow on pine needles, cypress leaves, water agar plus pine needles, and grew rapidly on PDA and OA mycelia but did not produce ascospores. On the medium of PDA plus pine needles, mycelia grew Produce as...

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Abstract

The invention provides a culture medium capable of inducing mango gummosis bacteria to generate ascospores. The culture medium is obtained by adding aseptic pine needles on the basis of a PDA culture medium. Mango gummosis bacteria hyphe blocks are inoculated to the culture medium, cultured for 1 week in the present of light at 25-28 DEG C and then transferred to a sill in a culture chamber at 25-28 DEG C under sunlight irradiation to continue to be cultured for 1-2 weeks, so that plenty of perithecia, asci and ascospores can be generated. The simple, economical and effective culture medium and a method are provided for obtaining plenty of mango gummosis bacteria ascospores through artificial culture, and technical support is provided for on biological characteristics, infection characteristics and other genetic research of mango gummosis pathogenic bacteria.

Description

technical field [0001] The invention belongs to the technical field of plant protection. Specifically, the invention relates to a culture medium capable of inducing the mango gummosis fungus to produce sexual ascospores and an application thereof. Background technique [0002] Fungi of the family Botryosphaeriaceae are widely distributed all over the world. There are many species and various host plants. They can not only cause tree canker as pathogens, but also lurk in host plant tissues as endophytic fungi. Mango gummosis is a common disease in mango growing areas in my country, and it occurs everywhere. The disease can be caused by Lasiodiplodia theobromae of the genus Botrytis, Fusicoccum aesculi and Neofusicoccum parvum, among which the main pathogen is Lasiodiplodia theobromae. [0003] So far, there are few reports on the infection characteristics and disease occurrence of mango gummosis, which brings great difficulties to the control of the disease. The fungus can ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12R1/645
CPCC12N1/14C12N3/00
Inventor 李其利莫贱友郭堂勋黄穗萍唐利华
Owner INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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