54results about How to "Separate application" patented technology

A suturing apparatus comprises an elongated body, at least one arms movable relative to the elongated body and at least one needle movable relative to the elongated body. The arm releasably holds an end portion of a length of suture. The arm has a sharp end portion adapted to pierce an inner surface of a wall of a biological structure and pass an end portion of the suture through the inner surface. The needle is adapted to pierce the inner surface of such biological structure at a location proximal to the location where the end portion of the suture was inserted. The needle captures an end portion of the suture from the arm and draws the end portion of the suture back through the inner surface. The end of the suture is then drawn out of the biological structure by removing the elongated body.

The present invention discloses a construction for key management module functionality which provides for secure encoding and decoding of messages which are up to two blocks long. A method for generating an encoded value having a first encoded value part and a second encoded value part from an unencoded value having a first unencoded value part and a second unencoded value part, comprising the steps of: obtaining an initialization vector; and generating the first and second encoded value parts. The first encoded value part is generated by: generating a first result by encrypting the first unencoded value part; generating a second result by performing an exclusive or operation on the first result and the second unencoded value part; generating a third result by performing an exclusive or operation on the second result and the initialization vector; generating a fourth result by encrypting the third result; generating a fifth result by performing an exclusive or operation on the fourth result and the first unencoded value part; and encrypting the fifth result. The second encoded value part is generated by encrypting the second result.

A suturing apparatus comprises an elongated body, at least one arms movable relative to the elongated body and at least one needle movable relative to the elongated body. The arm releasably holds an end portion of a length of suture. The arm has a sharp end portion adapted to pierce an inner surface of a wall of a biological structure and pass an end portion of the suture through the inner surface. The needle is adapted to pierce the inner surface of such biological structure at a location proximal to the location where the end portion of the suture was inserted. The needle captures an end portion of the suture from the arm and draws the end portion of the suture back through the inner surface. The end of the suture is then drawn out of the biological structure by removing the elongated body.

The invention discloses a preparation method and application of a graphene oxide composite membrane, and belongs to the technical field of gas separation membranes.The preparation method of the graphene oxide composite membrane comprises the steps that an aqueous dispersion liquid of graphene oxide is prepared through a Hummer method, and ultrasonic dispersion is conducted; metal salt or oxysalt is added, and heating and mixing are conducted; the surface of a micro-filtration membrane is covered with the prepared dispersion liquid through a vacuum filtration method; the membrane is put into a drying oven to be dried.The preparation method of the graphene oxide composite membrane has the advantages that the preparation method is simple, the thickness of the obtained membrane is smaller than 10 nm, the space between graphene sheet layers is adjustable, the obtained composite membrane is used for gas separation and has excellent comprehensive performance.Particularly, the graphene oxide composite membrane prepared from sodium tetraborate has high CO2 permeation flux and CO2 / CH4 and CO2 / N2 selectivity, and it is obtained through mixed gas tests under the humidifying condition that the CO2 permeation flux can reach 650 GPU, CO2 / CH4 selectivity is 75, and CO2 / N2 selectivity is 58.

The invention discloses a low-pressure high-flux chlorine-containing polymer nanofiltration membrane with a stable separation layer and a preparation method thereof. The nanofiltration membrane comprises a macroporous support layer and a dense function layer, wherein the macroporous support layer and the dense function layer are connected with each other by virtue of a C-N bond; the dense function layer is made of a positive, charged or amphoteric ionic cross-linking polyamine compound; the macroporous support layer is made of a chlorine-containing polymer. The preparation method of the filtration membrane comprises the following steps: firstly coating the surface of a chlorine-containing polymer ultrafiltration membrane with the polyamine compound, carrying out heat treatment, washing the ultrafiltration membrane, then dipping the ultrafiltration membrane in a mixed solution of a cross-linking agent and a charge reagent and carrying out heat treatment again. The membrane flux of the prepared polymer nanofiltration membrane can reach 60L / m<2>.h under the pressure of 0.3MPa; the retention rate on micromolecule dye and high-valence inorganic salt can reach 90%; the chlorine-containing polymer nanofiltration membrane can be in a flat fiber ultrafiltration membrane form or a hollow fiber ultrafiltration membrane form, and has good practical prospect.

The invention discloses a liquid-discharged gas-liquid separator comprises an upper cylinder, a lower cylinder, a liquid separating tube, an upper partition and a lower partition. The top of the upper cylinder is provided with a gas exhausting port used for exhausting separated gas. The bottom of the lower cylinder is provided with a liquid discharging port used for discharging separated liquid. The bottom of the upper cylinder and the top of the lower cylinder are connected in a sealed manner. The liquid separating tube is connected to the lower cylinder, a first end of the liquid separating tube is a straight tube, and a second end is an elbow bent along the inner wall of the lower cylinder. The upper partition is horizontally arranged in the upper cylinder, and a plurality of small holes or filters are annularly arranged in the surface of the inner wall of the upper partition adjacent to the upper cylinder. The lower partition is horizontally arranged at the bottom of the upper cylinder, and the center fo the lower cylinder is provided with an opening or filter. The invention further provides an injection system using the liquid-discharged gas-liquid separator and a cooling system bypassing with the gas inlet of an evaporator. Compared with a conventional gas-liquid separator, the liquid-discharged gas-liquid separator has the advantages that separating efficiency is high, pressure drop is low, and gas-liquid mixture can be separated and respectively discharged.

The present invention discloses a general-purpose hierarchical key management method and apparatus whose trusted operation, with respect to compromise of keying material, does not depend upon the controlling application. This enables designs that separate secure key management from the application-specific use of the keying material, and enables the rigorous evaluation of the key management module.

The invention discloses an unsteady sound field separation method, which is characterized by comprising the following steps of: arranging an auxiliary measuring surface S2 which is parallel to a measuring surface S1 and is delta z away from the measuring surface S1 in a tested sound field which has a target sound source Mo and an interference sound source Md simultaneously and radiates unsteady sound pressure signals, and synchronously measuring sound pressure time domain signals on the auxiliary measuring surface S2 and the measuring surface S1; performing two-dimensional space Fourier transform on the sound pressure time domain signals respectively to acquire a sound pressure wave number spectrum; separating a sound pressure wave number spectrum radiated only by the target sound source Mo on the measuring surface S1 through deconvolution operation by utilizing the sound pressure wave number spectrum and a time domain pulse response function; and performing two-dimensional space inverse Fourier transform on the separated sound pressure wave number spectrum, and finally acquiring a sound pressure time domain signal radiated only by the target sound source Mo on the measuring surface S1. By the method, the sound field can be separated in a spatial domain or a time domain; and the method is particularly suitable for separating an unsteady sound field in a non-free sound field environment.

The invention discloses an umbilical cord mesenchymal stem cell preparation and a preparation method and application thereof. Through exploration and verification by a large quantity of experiments, the 3rd-10th-generation umbilical cord mesenchymal stem cells are selected as raw materials, and by a method of dispersing tissue by a mechanical method and then performing treatment with mixed enzymes containing collagenase I, collagenase II and trypsin for a short time for many times, the vitality of the separated umbilical cord mesenchymal stem cells is guaranteed. A culture medium special for the umbilical cord mesenchymal stem cell preparation, containing DMEM / F12 (without phenol red), 10% FBS, 20 kinds of amino acids and 8 kinds of vitamins, is adopted, mixed liquor of repeated cell culture supernatant and cell lysate supernatant is collected, more umbilical cord mesenchymal stem cell active protein and active factors are obtained, the preparation cost is reduced, and industrial requirements for a stem cell product preparation are met. The prepared umbilical cord mesenchymal stem cell preparation can promote proliferation of epidermic cells and skin fibroblast, is safe and is free from toxic and side effects, an allergy does not exist, and when the umbilical cord mesenchymal stem cell preparation is applied to feature-beautifying skin-care products, the effects are notable.

The invention relates to a simple and convenient method for quickly separating microorganisms, in particular to a method for quickly separating ustilaginoidea virens, which belongs to the technical field of the microorganisms. The method comprises the following steps of: scratching and washing rice curve balls in sterilized water to remove contaminated microbes on a surface layer of the rice curve balls; sterilizing the rice curve balls for 30 to 60 seconds by using 75-percent ethanol solution, and then sterilizing the rice curve balls for 2 to 4 minutes by soaking the rice curve balls in 0.1-percent corrosive sublimate aqueous solution; washing the rice curve balls in the sterilized water, and then adding the rice curve balls into the sterilized water to smash the rice curve balls; using smashing solution to flush a separation Wakimoto culture medium; air-drying the water on the surface of the culture medium, and then putting a culture dish in a thermostat for dark culture at 28 DEG C; after the sixth day of the culture, picking yellow or white small colonies and white mycelia by using a sharp end picking needle, and moving the yellow or white small colonies and the white mycelia into a culture Wakimoto culture medium to perform purification culture for 10 days; and moving the ustilaginoidea virens to a PSA slant culture medium and culturing the ustilaginoidea virens for 10 days to obtain corresponding ustilaginoidea virens. The method can quickly and effectively separate a large amount of the ustilaginoidea virens from the rice curve balls in a short period, and can effectively the problems such as pollution, time waste, poor reliability, high purification difficulty and the like in other methods for separating the ustilaginoidea virens.

The invention discloses a method for directly separating CD<4+> and CD<8+> lymphocytes, lays a better foundation for the subsequent research on the CD<4+> and CD<8+> lymphocytes, and relates to the field of biomedicines. The method comprises steps of: multi-arm well and star-shaped polymer and mouse anti-human CD<4+> or CD<8+> monoclonal antibody covalent coupling, long-chain biotin molecule coating through utilizing a mouse anti-human CD<4+> or CD<8+> monoclonal antibody-modified multi-arm well and star-shaped polymer, peripheral blood sample CD<4+> and CD<8+> lymphocyte acquiring through utilizing a mouse anti-human CD<4+> or CD<8+> monoclonal antibody and long-chain biotin co-modified multi-arm well and star-shaped polymer, peripheral blood long-chain biotinylation multi-arm well and star-shaped polymer identifying and coupling through utilizing streptavidin-modified nano magnetic beads, captured CD<4+> and CD<8+> lymphocyte separating and suspending and the like. A suspension can be directly used for subsequent analysis; and compared with a conventional cell separating method, the method is suitable for magnetically separating complicated peripheral blood sample CD<4+> and CD<8+> lymphocytes, so that the peripheral blood sample CD<4+> and CD<8+> lymphocyte separation efficiency is increased.

The invention discloses a metal can cap separating mechanism, which comprises a framework, a feeding passage and a discharging passage, wherein the feeding passage and the discharging passage are arranged on the framework. A feeding conveying belt and a discharging conveying belt which are identical in conveying direction and driven by the same power mechanism are arranged below the feeding passage and the discharging passage, the moving speed of the discharging conveying belt is higher than that of the feeding conveying belt, a can cap separating section is arranged between the feeding conveying passage and the discharging conveying passage, the front end of the feeding conveying belt and the rear end of the discharging conveying belt form a staggering structure in the can cap separating section, and metal can cap toppling preventing devices are arranged on two sides of the passage where the discharging conveying belt is located. The metal can cap separating mechanism solves the problem that existing metal can cap separating equipment is poor in separating effect, has requirements for product materials and affection on product quality effectively, is suitable for separation of metal can caps made of different materials and applicable to high-speed production lines of metal can caps.

The invention relates to a device for continuously concentrating gelatin by membrane technology. The outlet of a rolled membrane module of the device is communicated with a first connector of a four-way joint; a second connector and a third connector of the four-way joint are respectively connected with an original gelatin tank and a thick gelatin tank; the inlet of the rolled membrane module is communicated with an outlet of a circulating pump; a sorption pump and a filter are successively connected in series between the original gelatin tank and the inlet of the circulating pump; a first stop valve is in series connection with a pipe between the sorption pump and the filter; a second stop valve is in series connection with a pipe between the original gelatin tank and the outlet of the rolled membrane module; a first butterfly valve is in series connection with a pipe between the outlet of the rolled membrane module and the thick gelatin tank; a second butterfly valve is connected in parallel between the inlet of the circulating pump and the fourth connector of the four-way joint; and the discharge hole at one side of the rolled membrane module is provided with a third butterfly valve. In the invention, the continuous gelatin concentration operation technology is applied to the gelatin concentration technique, and the device in the invention is used for replacing the conventional device for kieselguhr filter, ion exchange and high-temperature evaporation concentration processes.

The present invention relates to a gas solid cyclone separator. It includes the following several portions: cylinder body, cone body, ash bucket and gas-collecting chamber. It is characterized by that the wall of said ash bucket is made up by using porous metal filtering material. Said invention also provides the concrete working principle of said gas solid cyclone separator. Its separation effect can be up to above 99%.

The invention provides an adsorption concentration method of volatile organic matter in solution. The method comprises the following steps: performing adsorption to the organic solution by passing thesolution through a first adsorption column filled with absorbent so as to accumulate the organic matter into the absorbent; after the first adsorption column is saturated, passing the organic solution through a second adsorption column filled with absorbent for adsorption; blowing the first adsorption column with inert atmosphere to desorb and recover the adsorbed organic matter, then heating thefirst adsorption column for desorption and regeneration; after the second adsorption column is saturated, passing the organic solution through the regenerative first adsorption column for adsorption;meanwhile, blowing the second adsorption column with inert atmosphere so as to desorb and recover the absorbed organic matter, heating the second adsorption column for regeneration; and repeating theabove steps to ensure the whole adsorption process to keep continuous. The invention also provides a device for realizing the method of the invention.

The dynamic continuous sucking filter with cage filtering barrel as one mechanical equipment includes a cage filtering barrel, a material guiding spiral belt device, a gas crossing disc, a vibrator, a left joint, a right joint and an elastic bearing part. Technologically, the present invention features that the rotating cage filtering barrel makes the flexible filtering medium in periodical alternating operation state of filtering and washing. The material guiding spiral belt device rotating synchronously with the cage filtering barrel force the slurry forward spirally, and the filter cake material moves along one cylindrical circular helix in constant changing state for solid-liquid separation. The filter-aiding vibrator further raises the separating efficiency. The filtering operation is especially suitable for separating liquid material with low viscosity and solid material with poor moisture absorption. The filter medium may be back washed and may be replaced easily.

The invention belongs to the technical field of microbe culture and relates to a culture method of a cordyceps sinensis anamorphic hirsutella sinensis pure strain. The culture method comprises the following steps: A sampling: acquiring a wild cordyceps sinensis stroma of which ascospore is not ejected out, and storing; B separating: taking a strain in the cordyceps sinensis stroma, and inoculating into a flat plate under a sterile condition; C culturing, performing constant-temperature culture of the flat plate containing the strain in a culture box to obtain a hirsutella sinensis bacterial colony; D purifying, transferring the bacterial colony in the step C to a new flat plate for culture to obtain the hirsutella sinensis pure strain. By adoption of the culture method, the hirsutella sinensis pure strain can be obtained more easily, and the culture method is suitable for separating the cordyceps sinensis strain.

The invention discloses a preparation method and application of super-hydrophobic foam iron. The preparation method comprises the following steps: firstly, electro-depositing a high-entropy alloy on super-hydrophilic foam iron, then carrying out super-hydrophobic surface treatment to obtain super-hydrophobic foam iron, and then applying the super-hydrophobic foam iron to a designed oil-water separation device to carry out oil-water separation. The method is simple in preparation process, low in production cost, green, efficient and suitable for large-scale industrial production. The prepared super-hydrophobic foam iron has the characteristics of self-cleaning, pollution prevention, long-term corrosion resistance, high oil-water separation efficiency and the like, the static contact angle of the super-hydrophobic foam iron is larger than 150 degrees, and the rolling angle of the super-hydrophobic foam iron is smaller than 10 degrees. The designed oil-water separation device can realizecontinuous oil-water separation and is suitable for oil-water separation of light oil and heavy oil, and has high separation efficiency in the separation process.

The invention discloses an oil-water separation pipe with a hydrophobic and oleophylic surface and a preparation method and application thereof. The oil-water separation pipe comprises a metal net substrate, a conical array constructed on the metal net substrate and made of epoxy resin and copper-containing nanorods wrapping the surface of the conical array, the conical height of the conical arrayranges from micron to millimeter, the distance between every two adjacent cones ranges from microns to millimeters, and the surface of the oil-water separation pipe is hydrophobic. The oil-water separation pipe material with the hydrophobic and oleophylic surface does not contain toxic and harmful substances, and is environmentally friendly, easy to clean, capable of being repeatedly used and good in stability. When emulsion separation of surfactant-containing oily wastewater is carried out, the method has the advantages of good emulsion separation effect on surfactant-coated micron-sized oildrops, high separation speed, high oil-water separation efficiency, continuous separation, low cost, low energy consumption, no need of adding other chemical reagents and the like.

The invention discloses a dry method selective solid material separation device, and belongs to the technical field of material gravity separation methods. An outlet in the bottom of a fluidization separation bed of the separation device is connected with a high-density material storage bin through a conveying pipeline. An outlet in the top of the fluidization separation bed of the separation device is connected with a low-density material storage bin through an upper portion air channel. An air conveying main pipe connected with an air blower is provided with air conveying branch pipes. One air conveying branch pipe is connected with an outlet pipeline of a feeding device through an air connecting box. The other air conveying branch pipes are respectively connected with an air bellow at the bottom of the fluidization separation bed. According to the dry method selective solid material separation device, a physical method is applied to separation of solid mixed materials, the form of water-soluble substances in the materials can not be damaged, intrinsic physicochemical properties of the materials can be kept, the separated materials can be further utilized, secondary pollution is avoided in the whole separation process, and the dry method selective solid material separation device has the best environmental-friendly effect. Meanwhile, the dry method selective solid material separation device is simple in structure, easy and convenient to operate and manage, free of adding other separation media, low in cost, and applicable to the separation of a majority of materials with different densities.

The invention discloses a three-port optical circulator, comprising a first port, a second port and a third port and used for transmitting light incident from the first port to the second port and transmitting light incident from the second port to the third port. The optical circulator further comprises a polarization conversion module, a nonreciprocal polarization optical rotation module, a polarization beam splitter prism and a reflection prism. Compared with the prior art, a reflection-type folded optical path is adopted; and compared with a traditional optical isolator, the optical circulator has the advantages of being shorter and smaller in boundary dimension, simple in optical path structure, easy to manufacture in batches and higher in isolation degree in design, and further meetsapplication requirements on a single-fiber bidirectional transmission module in the field of digital communication.

The invention provides a building block part pulling device. The building block part pulling device comprises a handle body and is characterized in that an annular building block part clamping mechanism is arranged at the front end of the handle body, wherein the annular building block part clamping mechanism comprises a columnar inner clamping part (201) and at least one an outer clamping part matched with the columnar inner clamping part for clamping annular building bock parts; the rear end of the columnar inner clamping part and the rear end of the outer clamping part are respectively connected to the front end of the handle body. Compared with the prior art, the building block part pulling device has the beneficial effects that the annular building block part clamping mechanism is arranged, so that the annular building block parts can be conveniently pulled out; a separation shovel is suitable to the situation that a thin and long gap is formed in a connection position between twobuilding block parts; a compounding hole is applied to the building block parts with annular steps at the tops; a cross-shaped shaft is applied to axle-shaped parts; a hole part circular truncated cone plugging and pulling part is applied to hole type building block parts. The building block part pulling device is ingenious in structural design, convenient to operate and convenient and quick to use, and is especially applied to separation of various building block parts.

The invention relates to an oil-water separator for separating two-phase mixed liquid and a method for separating volatile oil and water by utilizing the separator. The separator is particularly suitable for the oil-water separation in two-phase liquid containing more solid suspended matters and comprises a base, a collector, a filter screen and an extracting device, wherein the base is suspended in two-phase mixed liquid; the collector is fixed and supported by the base and used for collecting low-density liquid; the filter screen surrounds the collector; the extracting device is used for extracting the liquid in the collector; the collector is provided with a barrel-shaped collector body; and the upper end of the side wall of the collector body is positioned between the liquid level and the interface of the two-phase mixed liquid or the side wall of the collector body is provided with an overflow opening, and the lower end surface of the overflow opening is positioned between the liquid level and the interface of the two-phase mixed liquid. The invention has simple installation and operation and good separating effect.

The invention discloses a method for removing a residual solvent in a lycopene oil resin, which belongs to the technical field of separating and purifying the residual solvent in the food processing. An extraction of the lycopene oil resin to be treated is added into a material bottle; after checking and confirming of an airtight system, a vacuum pump is started to degas the material; after a numerical value of a vacuum degree is stabilized, the procedure of degassing is completed. Scraper rotational speed, feeding velocity, pressure of molecular distillation, distillation temperature and condensing temperature are set, a material valve is opened to allow the material to flow into a distillation body so as to undergo the molecular distillation, impurities contained in the oil resin enter a distillate component, and the remaining residue component, namely the purified lycopene oil resin is collected. Because the molecular distillation technology is adopted, the method can not only remove the residual solvent in the lycopene oil resin and reduce contents of other impurities in the oil resin, but also can improve safety of the lycopene oil resin and density of lycopene, thereby contributing to strengthening the activity of the lycopene.

The invention relates to the technical field of vascular cell separation, in particular to a method for separating and enriching vascular endothelial progenitor cells from peripheral blood. The method comprises the steps of obtaining a peripheral blood sample, and using PBS phosphate buffer for dilution; adding diluted blood into a hydroxyethyl starch solution, performing centrifuging and sucking monocular cell sap of a middle albuginea cell layer; using the PBS phosphate buffer for dilution, performing centrifuging, abandoning a supernatant, and performing resuspension on residues through a nutrition solution; performing culture transferring. The method is high in separation efficiency, adopts few parenchyma cells, only needs a small number of experiment instruments, is good in separation effect and has the repeatability.

The invention discloses a method for enriching and separating human peripheral blood CD34<+> and CD91<+> stem cells, which provides basis for the subsequent researches on CD34<+> and CD91<+> stem cells better, and relates to the field of biomedicine. The method comprises the following steps of: covalently coupling a multi-arm well star-shaped polymer with a mouse anti-human CD34<+> or CD91<+> monoclonal antibody; coating long-chain biotin molecules on the multi-arm well star-shaped polymer modified by the mouse anti-human CD34<+> or CD91<+> monoclonal antibody; capturing the CD34<+> and CD91<+> stem cells in a peripheral blood sample by the multi-arm well star-shaped polymer modified by the mouse anti-human CD34<+> or CD91<+> monoclonal antibody; identifying and coupling the long-chain biotinylated well star-shaped polymer in the peripheral blood by nanometre magnetic beads modified by streptavidin; separating and re-suspending the captured CD34<+> and CD91<+> stem cells, and the like, wherein the re-suspension can be directly used for subsequent analysis. Compared with the traditional cell separation method, the method is more suitable for performing magnetic separation on the CD34<+> and CD91<+> stem cells in the complex peripheral blood sample, and capable of increasing the separation efficiency for the CD34<+> and CD91<+> stem cells in the peripheral blood sample.

The invention discloses a method for separating hematopoietic stem cells (HSC) enriched in peripheral blood, aims to better supply subsequent research on the HSC, and relates to the field of biomedicine. The method comprises the following steps of: performing covalent coupling on dendritic hyperbranched polymers and anti-rat human HSC monoclonal antibodies, wrapping the dendritic hyperbranched polymers modified by the anti-rat human HSC monoclonal antibodies by long-chain biotin molecules, capturing the HSC in a peripheral blood sample by the dendritic hyperbranched polymers co-modified by the anti-rat human HSC monoclonal antibodies and the long-chain biotins, identifying nano magnetic beads modified by streptavidin, making the nano magnetic beads be coupled with the long-chain biotin dendritic hyperbranched polymers in the peripheral blood, separating the captured HSC, performing heavy suspension on the captured HSC and the like, wherein heavy suspension liquid can be directly subjected to subsequent analysis. Compared with the conventional cell separation method, the method disclosed by the invention is more suitable for performing magnetic separation on the HSC in the complicated peripheral blood sample and the efficiency of separating the HSC from the peripheral blood sample is improved.

The invention discloses a liquid-discharged gas-liquid separator comprises an upper cylinder, a lower cylinder, a liquid separating tube, an upper partition and a lower partition. The top of the upper cylinder is provided with a gas exhausting port used for exhausting separated gas. The bottom of the lower cylinder is provided with a liquid discharging port used for discharging separated liquid. The bottom of the upper cylinder and the top of the lower cylinder are connected in a sealed manner. The liquid separating tube is connected to the lower cylinder, a first end of the liquid separating tube is a straight tube, and a second end is an elbow bent along the inner wall of the lower cylinder. The upper partition is horizontally arranged in the upper cylinder, and a plurality of small holes or filters are annularly arranged in the surface of the inner wall of the upper partition adjacent to the upper cylinder. The lower partition is horizontally arranged at the bottom of the upper cylinder, and the center fo the lower cylinder is provided with an opening or filter. The invention further provides an injection system using the liquid-discharged gas-liquid separator and a cooling system bypassing with the gas inlet of an evaporator. Compared with a conventional gas-liquid separator, the liquid-discharged gas-liquid separator has the advantages that separating efficiency is high, pressure drop is low, and gas-liquid mixture can be separated and respectively discharged.

The invention discloses a method for separating hematopoietic stem cells (HSC) enriched in peripheral blood, aims to better supply subsequent research on the HSC, and relates to the field of biomedicine. The method comprises the following steps of: performing covalent coupling on dendritic hyperbranched polymers and anti-rat human HSC monoclonal antibodies, wrapping the dendritic hyperbranched polymers modified by the anti-rat human HSC monoclonal antibodies by long-chain biotin molecules, capturing the HSC in a peripheral blood sample by the dendritic hyperbranched polymers co-modified by the anti-rat human HSC monoclonal antibodies and the long-chain biotins, identifying nano magnetic beads modified by streptavidin, making the nano magnetic beads be coupled with the long-chain biotin dendritic hyperbranched polymers in the peripheral blood, separating the captured HSC, performing heavy suspension on the captured HSC and the like, wherein heavy suspension liquid can be directly subjected to subsequent analysis. Compared with the conventional cell separation method, the method disclosed by the invention is more suitable for performing magnetic separation on the HSC in the complicated peripheral blood sample and the efficiency of separating the HSC from the peripheral blood sample is improved.