Simple and convenient method for quickly separating ustilaginoidea virens

Abstract

The invention relates to a simple and convenient method for quickly separating microorganisms, in particular to a method for quickly separating ustilaginoidea virens, which belongs to the technical field of the microorganisms. The method comprises the following steps of: scratching and washing rice curve balls in sterilized water to remove contaminated microbes on a surface layer of the rice curve balls; sterilizing the rice curve balls for 30 to 60 seconds by using 75-percent ethanol solution, and then sterilizing the rice curve balls for 2 to 4 minutes by soaking the rice curve balls in 0.1-percent corrosive sublimate aqueous solution; washing the rice curve balls in the sterilized water, and then adding the rice curve balls into the sterilized water to smash the rice curve balls; using smashing solution to flush a separation Wakimoto culture medium; air-drying the water on the surface of the culture medium, and then putting a culture dish in a thermostat for dark culture at 28 DEG C; after the sixth day of the culture, picking yellow or white small colonies and white mycelia by using a sharp end picking needle, and moving the yellow or white small colonies and the white mycelia into a culture Wakimoto culture medium to perform purification culture for 10 days; and moving the ustilaginoidea virens to a PSA slant culture medium and culturing the ustilaginoidea virens for 10 days to obtain corresponding ustilaginoidea virens. The method can quickly and effectively separate a large amount of the ustilaginoidea virens from the rice curve balls in a short period, and can effectively the problems such as pollution, time waste, poor reliability, high purification difficulty and the like in other methods for separating the ustilaginoidea virens.

Description

technical field [0001] The invention relates to a simple and rapid method for isolating microorganisms, in particular to a method for rapidly isolating rice smut bacteria, and belongs to the technical field of microorganisms. Background technique [0002] Rice false smut is a worldwide fungal disease of rice, which damages the panicle of rice. The sex is Claviceps oryzaesativae Has. in the Ascomycota genus, and the anamorphic is the Deuteromycota Chlorides (Claviceps oryzaesativae Has. Ustilaginoideavirens (Cooke) Takahashi), in many countries and regions such as Asia, Africa and America. Rice false smut not only harms rice, affecting yield and rice quality, but also contains toxins that are highly toxic to humans and animals in the rice balls and pathogens mixed in the diseased rice, and has become an important disease in rice production. [0003] In the glumes infected with rice smut, with the development of white hyphae, the disease grains gradually expand, and a white t...

AI Technical Summary

Problems solved by technology

However, in the actual operation process of many researchers, it is generally found that although the rice koji ball inner layer tissue separation method can be used to isolate rice smut bacteria, the culture time is long, the separation speed is slow, and the success rate is low, because it is often accompanied by The emergence of other miscellaneous bacteria such as bacteria, fungi, and miscellaneous bacteria similar to rice smut, such as Nigrospora oryzae, affects the effect of further isolation and purification of rice koji

Chlamydospore suspension method, because the chlamydospores mixed in the medium must germinate into hyphae and pass through the medium, prolonging the time for the chlamydospores to form a single colony, which will easily lead to the rapid growth of miscellaneous bacteria covering a small amount Single colonies in the shape of steamed buns increase the difficulty of separation and purification

Spore shake-off method, because the surface of rice koji is not disinfected, a large number of bacteria on the surface of rice koji are easy to grow rapidly on the surface of the medium, which affects the germination and growth of chlamydospores and the purification of colonies

The sclerotium isolation method of rice smut is rare in southern rice areas, so this method is of little significance for the study of southern rice smut fungus flora

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