Culture method of cordyceps sinensis anamorphic hirsutella sinensis pure strain

A technology of Trichosporium sinensis and Cordyceps sinensis, which is applied in the field of cultivation of asexual type Trichospora sinensis pure strains of Cordyceps sinensis, can solve the problems of low success rate of Trichospora strains, etc., and achieves convenient and quick method, growth promotion, and success rate. high effect

Inactive Publication Date: 2016-10-12
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Based on past experience, the success rate of isolating Mortierella species from Cordyceps sinensis tissue or ascospore catapults outside the Cordyceps producing area is very low

Method used

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  • Culture method of cordyceps sinensis anamorphic hirsutella sinensis pure strain
  • Culture method of cordyceps sinensis anamorphic hirsutella sinensis pure strain
  • Culture method of cordyceps sinensis anamorphic hirsutella sinensis pure strain

Examples

Experimental program
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Embodiment 1

[0030] In this embodiment, the preparation method of the plate is as follows: peel and wash the potatoes, weigh 300g potatoes, cut them into small pieces, boil them with water, filter them, add 15-20g of agar and 20g of glucose, continue heating, and stir to mix Mix well, cool down, add silkworm chrysalis powder extract, yeast powder extract and corn flour extract, add water to make the volume to 1000mL, PDA medium, sterilize after subpackaging, put the sterilized PDA medium in a sterile environment Pour it down into a petri dish and form a flat plate after solidification. The medium was cultured in a 37°C incubator for 24 hours, and poured into a petri dish after confirming the aseptic growth. Silkworm chrysalis powder extract and yeast powder extract are formed by adding water to commercially available silkworm chrysalis powder extracts, yeast powder extracts, and corn flour extracts. The quality standards of commercially available products meet the Chinese Pharmacopoeia. T...

Embodiment 2

[0040] This example is basically the same as Example 1, except that in Step B, the inoculation method is as follows: After the ascospores are shot for 7 days, the remaining cores of the ascospores are sterilized with alcohol, and the worms are cut from the junction of the ascospores and the worm body. Under aseptic conditions, use sterilized tweezers to peel off the brown mycelium tissue of the outer layer of the sub-seat until the off-white inner mycelium is exposed, tear off a little white mycelium with tweezers, cut into small pieces with sterilized scissors, and put Into the plate culture for steps C and D.

Embodiment 3

[0042] This example is basically the same as Example 1, except that in Step B, the inoculation method is as follows: After the ascospores are shot for 7 days, the remaining cores of the ascospores are sterilized with alcohol, and the worms are cut from the junction of the ascospores and the worm body. After sterilizing the outside of the worm body, cut it open with a knife, take a small amount of internal tissue of the worm body and put it into a plate for culture to carry out steps C and D.

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Abstract

The invention belongs to the technical field of microbe culture and relates to a culture method of a cordyceps sinensis anamorphic hirsutella sinensis pure strain. The culture method comprises the following steps: A sampling: acquiring a wild cordyceps sinensis stroma of which ascospore is not ejected out, and storing; B separating: taking a strain in the cordyceps sinensis stroma, and inoculating into a flat plate under a sterile condition; C culturing, performing constant-temperature culture of the flat plate containing the strain in a culture box to obtain a hirsutella sinensis bacterial colony; D purifying, transferring the bacterial colony in the step C to a new flat plate for culture to obtain the hirsutella sinensis pure strain. By adoption of the culture method, the hirsutella sinensis pure strain can be obtained more easily, and the culture method is suitable for separating the cordyceps sinensis strain.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, and relates to a method for cultivating a pure strain of Cordyceps sinensis anamorphic Mortierella sinensis. Background technique [0002] Cordyceps sinensis is a unique and precious specialty in my country, also known as Cordyceps sinensis. Because of its powerful health care effects, it is as famous as deer antler and ginseng, and is listed among the three major supplements. At the beginning of the research, some people once classified Cordyceps sinensis into the order Hypocreeales, but according to the recent systematic classification, Cordyceps sinensis should belong to Fungi, Ascomycota, Ascomycetes, and Faecalibacterium. Class Sordariomycetidae, Hypocreales, Clavicipifaceae, Cordyceps. Therefore, Cordyceps sinensis is a compound of the sub-seat and the larva corpse of the fungus Cordyceps sinensis of the family Ergotaceae parasitizing on the overwintering larvae of the bat f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 林海萍朱旭伟虞方伯龙正海张心齐赵学
Owner ZHEJIANG FORESTRY UNIVERSITY
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