179 results about "Cordyceps ophioglossoides" patented technology

The invention provides a total artificial method for cultivating the cordyceps sinensis, and belongs to the field of the total artificial cultivation of the cordyceps sinensis. The method comprises the steps of: a. preparing a Chinese hirsutella suspension; b. acquiring, propagating and raising the host insect; c. infecting: taking the hepialus larva obtained by the step b, and soaking the suspension of the step a by a needle-shaped apparatus with the diameter which is less than 0.2mm at one end to prick the hepialus larva to complete the infection; and d. continuously raising. After the method is used, the improper mortality of the chrysalis can be reduced, and the eclosion rate of the hepialus is improved; the mating rate of the hepialus can be improved; and the infection rate of the Chinese hirsutell to the host hepialus larvae can be greatly improved, and the total artificial industrial production of the cordyceps sinensis can be realized.

The invention discloses a method for culturing selenium-enriched Cordyceps militaris, which is to increase the selenium content in a culture medium to 50mg/L by using organic selenium yeasts instead of inorganic seleninic acid and sodium selenite, which infect the growth of hyphae, so as to ensure the selenium content in the cultured selenium-enriched Cordyceps militaris reaches 35 to 50mg/L. In the invention, the organic selenium yeasts which exert the lest inhibition effect on the growth of the Cordyceps militaris are adopted to increase the selenium content in the culture medium to 50mg/l, the selenium content in the cultured selenium-enriched Cordyceps militaris reaches 35 to 50mg/kg, which is 2 times higher than that of the Cordyceps militaris cultured in a culture medium added with inorganic selenium, and the yield of Cordyceps militaris is high.

A manufacturing method for cordyceps sinensis (Berk.) sacc polysaccharide relates to the technical field of food microorganism application, and comprises the following steps: weighing raw materials, wherein rice bran is 1-3 g per 100 ml, bran is 1-3 g per 100 ml, potassium dihydrogen phosphate is 0.09-0.18 g per 100 ml, and magnesium sulphate is 0.04-0.08 g per 100 ml; material filling with natural pH after adding required water, and sterilizing and cooking at the temperature of 121 DEG C for 30 min; inoculating cordyceps sinensis (Berk.) sacc CCTCCM2013285 liquid seed after cooling, wherein the inoculation amount is 8-10%, the fermentation temperature is 23-28 DEG C, and the fermentation time is 4-7 d; respectively obtaining exopolysaccharides and mycelia polysaccharide after conducting deproteinization, alcohol precipitation, centrifugal separation, and vacuum drying on centrifugal separation liquid extracted through breaking cell wall hot water of fermentation material centrifugal separation liquid and hypha. The method better realizes the purpose that rice bran and bran are efficiently and high-valuedly transformed to cordyceps sinensis (Berk.) sacc polysaccharide after being subjected to liquid state fermentation.

A technique for culturing cordyceps by insect includes such steps as collecting fresh wild cordyceps, tissue separation to obtain its bacterial strain, reproduction, culturing slant strain and liquidbacteria, choosing host insect or host insect surrogate, spraying bacterial liquid for infecting the host insect, and culturing in the condition stimulating the wild environment. Its advantages are low cost and high effect.

The invention discloses a method for extracting and purifying polysaccharide from a fermented mycelium of Cordyceps sinensis. The method comprises the following steps of: using the fermented mycelium of Cordyceps sinensis as a raw material to produce crude polysaccharide of the Cordyceps sinensis via degreasing, aqueous extraction, concentration and alcohol precipitation; then, redissolving the crude polysaccharide into distilled water, deproteinizing the crude polysaccharide by Sevag, decoloring the deproteinized crude polysaccharide by H2O2, dialyzing the decolored crude polysaccharide, and precipitating the dialyzed crude polysaccharide by alcohol to produce primarily purified polysaccharide; finally, purifying the primarily purified polysaccharide by ion-exchange chromatography, collecting different produced compositions stepwisely, dialyzing the compositions, precipitating the dialyzed compositions by alcohol, drying the precipitated compositions to produce the polysaccharide of the Cordyceps sinensis after the precipitated compositions are orderly washed by absolute ethyl alcohol, acetone, aether and so on. In the produced polysaccharide of the Cordyceps sinensis, MPS2 is identified to be homogeneous polysaccharide by ultraviolet absorption spectrum and gel chromatography, with high content of total sugar. The method has the advantages of simple and convenient extraction steps and low cost.

The invention supplies a high activity wild Chinese caterpillar fungus strain and the artificial planting method. The Chinese caterpillar fungus strain is GDIM_C050423 CCTCC M 206051 that could produce fruiting body with high activity material. The method includes mother strain making, first level liquid strain making, second level liquid strain making, inoculating, artificial plating and gathering. The fruiting body has the feature of rod shaped, no branch or only has short branches at the top, and tip shaped top. It has abundant nutritive value and health protection function. It contains over 39.2% cordycepic acid.

The invention provides a new technological method for preparing and industrially culturing aweto strains. The method comprises the following steps of: using young silkworms of 2 years as a host of an aweto culture medium, preparing strains by separating the tissue of wild awetos, dying the young silkworms, providing biological conditions suitable for aweto growth, and culturing aweto products with the shapes of wild awetos and the similar contents of various food mass. The invention has simple and convenient operation process, high yield rate and low culture cost.

The invention provides a strain material adopting cordyceps sinensis mycoderma instead of the hirsutella sinensis spore bacterium as the cordyceps sinensis host infection bacterium. The strain material used for infecting hosts during cordyceps sinensis host raising is the cordyceps sinensis mycoderma. The strain material has the following beneficial effects: a method has the advantage of lower preparation cost of the strain material; and more importantly, by using the cordyceps sinensis mycoderma as the strain material, the survival rates and infection rates of the cordyceps sinensis hosts are obviously improved compared with the survival rates and infection rates of the hirsutella sinensis spore bacterium inoculated liquid and the host larvae.

The invention discloses a culturing method of cordyceps militaris rich in effective component (schisandra chinensis) and relates a culturing method of cordyceps militaris, and the method can be used for simplifying traditional Chinese medicine for compatibility, and improving the utilization rate of the cordyceps militaris. The method comprises the following steps: 1. activating a strain; 2. preparing a liquid strain; 3. smashing and diluting the liquid strain, and inoculating the smashed and diluted strain to a solid culturing medium of the cordyceps militaris rich in schisandra chinensis, and carrying out dark culture to obtain a mycelium; culturing the mycelium under illumination to obtain a mature sporocarp; continuously culturing until a stroma grows to 4-8cm and an orange power substance appears on the surface of the stroma; and harvesting. The method provided by the invention has the following advantages: based on the mutual effect of the components in the schisandra chinensis and cordyceps sinensis growth metabolism, the contents of adenosine and cordycepin are improved, and simultaneously a cordyceps sinensis sporocarp with an effective component (schisandra chinensis) is generated, thereby playing roles in utilizing the cordyceps militaris and carrying out compatibility on the cordyceps militaris, simplifying the compatible method of the schisandra chinensis and the cordyceps militaris, and improving the nutrition and medical value of the schisandra chinensis.

The invention relates to a culture method of cordyceps sinensis, in particular to a large-scale culture method of cordyceps sinensis. The method comprises the following steps: after inoculations, culture vessels are arranged and stacked in tissue culture rooms on a controllable optical culture shelf; the culture process contains the steps of non-illuminating culture and illuminating culture; and in the illuminating culture step, the controllable optical culture shelf can be utilized to adjust the illumination intensity and the illumination period. By adopting the method in the invention, a serie of problems of the existing large-scale culture method of cordyceps sinensis that as the illumination intensity is not uniform and the illumination is not controllable, the normal growth demand of cordyceps sinensis can not be satisfied, the grass yield of cordyceps sinensis is low, the grass shape is poor, the contents of active ingredients in a fruiting body are low, the biological conversion rate of cordyceps sinensis is low and the like can be solved. In addition, in the method in the invention, the illumination intensity range and illumination period of the grass-growing management stage of the cordyceps sinensis culture can be adjusted to increase the contents of active ingredients in the fruiting body and the accumulation of biomass in the fruiting body.

The present invention provides one method of culturing Northern Cordyceps in a airlift bioreactor in large scale and separating and extracting Cordyceps extract. Seed for fermentation is cultured in MS culture medium and then transferred to 20 L bioreactor for large scale culture. The culture liquid in the bioreactor is then separated and purified to obtain product with Cordyceps extract content up to 46.25 % and purity of 99 %. The process of the present invention has low production cost and no environmental pollution.

A cultivation method of selenium-enriched Cordyceps militaris mother fungi belongs to the technical field of the cultivation of Cordyceps militaris. The cultivation method comprises the following steps: 1) cultivating selenium-enriched strains on a test tube slant; 2) cultivating selenium-enriched Cordyceps militaris liquid strains; and 3) cultivating the selenium-enriched Cordyceps militaris. The cultivation method uses organic selenium yeast which has the lowest inhibitory effect on the growth of Cordyceps militaris, thus the selenium content of the culture medium is up to 50mg/L, the selenium content of the cultivated Cordyceps militaris is up to 35-50mg/kg which is triple of the selenium content of Cordyceps militaris with additional inorganic selenium, and the yield is increased by 20%.

The present invention discloses a method used for artificially cultivating Chinese caterpillar fungus in a low altitude region, and relates to various technology characteristics such as host engineering, bacterial strain engineering, infect engineering and germination engineering and the like, the various technologies are effectively combined, steps are implemented accurately, and finally the Chinese caterpillar fungus, components and appearance of which are identical with wild Chinese caterpillar fungus, can be finally cultivated successfully. A sporocarp growth hastening confirmation step is newly added in a bacterial strain preparation step, qualified bacterial strain is ensured to be separated, and production efficiency and yield are improved. In addition, a specific worm cultivation mode is employed, and generation circulation of worm body cultivation is ensured. Comparing the Chinese caterpillar fungus artificially cultivated by the technology with the wild Chinese caterpillar fungus, similarity proportions of protein components exceed 95%, and the method has wide market and application prospects.

The invention relates to the field of microorganisms, and discloses a cordyceps sinensis fruiting body and a cultivating method thereof. The cultivating method includes the steps that hirsutella sinensis is inoculated onto a solid culture medium, fungal colony cultivation is performed for 35-55 days under the conditions that sunlight is kept out, the temperature is at 14-20 DEG C and the relative humidity is 45-60%, and then the fruiting body is induced under the conditions that the sunlight is kept out, the oxygen concentration is 10-15%, the temperature is 0-17 DEG C and the relative humidity is 70-90% until the fruiting body grows. According to the method, the hirsutella sinensis is inoculated onto the specific culture medium, the conditions of humidity, temperature and the like at different growth stages of the hirsutella sinensis are controlled, the real cordyceps sinensis fruiting body is cultivated in the low-temperature, lucifugal and oxygen-poor environment, and the immunomodulatory effect and the active material content of the cordyceps sinensis fruiting body are almost the same as those of a wild cordyceps sinensis fruiting body.

The invention provides a preparation method of selenium-rich cordyceps sinensis mycelia. The method comprises the following steps of: inoculating cordyceps sinensis stains in a liquid fermentation culture medium with brewer's grains as a matrix; and culturing for 4-8 days under the conditions of 20-30 DEG C and 120-200 r/min to obtain the selenium-rich cordyceps sinensis mycelia in fermentation liquor, wherein the liquid fermentation culture medium is prepared from the following components: 10-50 g/L of brewer's grains, 10-30 mg/L of Na2SeO3 and water serving as a solvent with pH (Potential of Hydrogen) of 4-8. The obtained fermentation liquor can be further separated by the conventional method to obtain the selenium-rich cordyceps sinensis mycelia. Compared with the prior art, the method has the advantages of mild reaction conditions, low production cost, less equipment investment and easiness in realization of industrialization, and the obtained mycelia have stronger biological activity, so that a breakthrough is expected to be made in prevention and treatment of animal diseases.

The aweto sporophore culturing process includes three stages: spawn culturing, spawn rejuvenation and commercial sporophore culturing. The culture medium for rejuvenation culturing and the culture medium for sporophore culturing are magnetizing treated. The commercial sporophore culturing includes three steps: low temperature culturing, temperature difference culturing, and constant temperature culturing. The present invention adopts wild aweto spore as the propagation material to maintain the excellent features of wild aweto effectively; and the magnetizing treatment on the culture medium can excite the activity of culture medium, promote spawn growth and raise biological efficiency greatly.

The invention relates to a production technology of ecological cordyceps sinensis wine. According to the production process of the ecological cordyceps sinensis wine, cordyceps sinensis and high-quality white spirits are taken as main raw materials, extracts of officinal, edible or officinal and edible plants are blended into flavoured spirit; or white cordyceps sinensis wine is directly made, and officinal, edible or officinal and edible plants are used for seasoning and blending. The ecological cordyceps sinensis wine produced by the production process has the characteristics that active ingredients in wine are rich and measurable and the wine contains no artificial colouring matter or non-natural compound, so that the production technology of the ecological cordyceps sinensis wine can meet ecological requirements.

The invention relates to a preparation method for an Ophiocordyceps sinensis strain material and a method for infecting Thitarodes larvae with the Ophiocordyceps sinensis strain material. According to the methods, the principle that the imperfect stage of Ophiocordyceps sinensis--Hirsutella sinensis and plant tissue form a symbiont is employed; a prepared Ophiocordyceps sinensis strain source is inoculated onto the plant tissue; proper conditions are created to promote symbiosis of the Hirsutella sinensis and the plant tissue so as to activate infection activity of the Hirsutella sinensis on the host Thitarodes larvae; the activated Ophiocordyceps sinensis strain Hirsutella sinensis forms the fungus-plant symbiont with the plant tissue; and the fungus-plant symbiont is used as the strain material with high infection activity on the host Thitarodes larvae. The infection rate is up to 85.6 to 98.9% by directly feeding the Thitarodes larvae with the prepared strain material for infection.

The invention discloses a mushroom soup and a cooking method thereof. The raw mateirals of the mushroom soup include chanterelle, toadstool, fungus suillus, chanterelle, boletus erythropus, sarcodon aspratum, bamboo fungus, gloeostereum insarnatum, tricholoma matsutake, tremella auramtialba, club fungi, cepe, hericium erinaceus, pleurotus eryngii, gomphidius rutilus, cordyceps sinensis, helvella elastica and flammulina velutipes. The cooking method comprises the following steps: soaking the raw materials, heating for more than three hours under big fire, wherein during the heating process, the soup is stirred uniformly for many times and floating foams on the surface are removed in time; after foam is removed, further heating under soft fire, wherein the soap is stirred for many times during the heating process; stopping heating, covering with a cover, standing for precipitation, thus obtaining the mixed mushroom soup. In the raw materials, the mushrooms are matched scientifically and reasonably, thus the mushroom soup is suitable for various people. In the cooking method, characteristics of various mushrooms are put into a full play, the taste and the fragrance components are mixed together, the nutrition and the taste are the best, and the mushroom soup has extremely high northern regional features, specific mushroom smell is kept, the efficacies of improving the immunity and the like are achieved, and the mushroom soup is suitable for people with various physiques.

The invention relates to a cultivation method of cordyceps militaris. A culture medium comprises the following nutrition ingredients in percentage by weight: 87% of long-shaped rice, 4.3% of silkworm chrysalis meal, 4.6% of glucose, 3.2% of peptone, 0.25% of monopotassium phosphate, 0.25% of magnesium sulfate, 0.25% of ammonium citrate and 0.15% of VB1. The cultivation method comprises the steps that the nutrition ingredients and water are mixed, wherein the water content is controlled at 60%, and the pH value is controlled to be between 6.0-6.5; the culture medium is loaded into boxes, and is subjected to sterilization and inoculation, and dark culture is carried out for 10 days for generating hypha at the temperature of 20-21 DEG C and the relative humidity between 55%-60%; the single-layer cordyceps militaris fungus boxes full of hypha are arrayed on a cultivation frame, transparent moisturizing materials cover the fungus boxes, ventilation and moisture increment are carried out by using an air regenerating device and humidifying equipment, the fungus boxes are exposed to light for color change, after 3 days the temperature difference is increased for promoting anlage formation, and harvesting is carried out when the cordyceps militaris grows to about 5cm long. Compared with the existing cordyceps militaris, the cordyceps militaris cultivated according to the method disclosed by the invention has the advantages that a part of technical indexes surpass the technical indexes of the existing cordyceps militaris, and the edible and medicinal values are high.

The invention provides a cassava silkworm coydyceps militaris (L.) link cultivated by taking cassava silkworm chrysalis as a host and a cultivating method thereof. The cassava silkworm coydyceps militaris (L.) link is specifically characterized in that: the cassava silkworm chrysalis is yellow brown or dark brown mycelium with a slightly yellow body surface; and rod-like or branched orange-yellow fruiting bodies are grown on the body surface of the silkworm chrysalis. The cassava silkworm coydyceps militaris (L.) link is cultivated by the following steps of: cultivating the strain of the coydyceps militaris (L.) link into liquid strain of the coydyceps militaris (L.) link; injecting the strain into the body of the cassava silkworm chrysalis by using a sterilized syringe; and then controlling the environment conditions such as the temperature, the moisture, the light and the like to cultivate the cassava silkworm coydyceps militaris (L.) link. The cassava silkworm coydyceps militaris (L.) link product has various active ingredients and nutritional ingredients, has a similar function to that of cordyceps sinensis, and can be used as a medicament, a health-care product and food; and the cultivating method is simple, has short period and low cost and is a practical method for developing the novel coydyceps militaris.

The invention relates to a method for sustainable high yielding of wild cordyceps sinensis in alpine regions. Advantaged climate conditions of cordyceps sinensis source areas are utilized; beginning from initial vegetation construction, water and electricity facilities are established, and a protective device is erected, so as to enrich food resources of cordyceps sinensis hosts and reduce natural enemies against the hosts. insect sources and fungus sources are introduced, and wild cultivation of cordyceps sinensis is achieved. A method of opening holes and placing eggs into the holes is adopted for introduction of the insect sources. A method of transplanting fresh excellent cordyceps sinensis is adopted for the introduction of the fungus sources, so that insects and fungi grow in a natural operation mode and undergo injection to form cordyceps sinensis. Cordyceps sinensis is controlled to be dug reasonably and orderly, and sustainable development of cordyceps sinensis is maintained.

The invention discloses a high-cordycepin-content rich-selenium cordyceps sinensis cultivation method. The high-cordycepin-content rich-selenium cordyceps sinensis cultivation method comprises the following steps of 1 cordyceps taishanensis primary solid cultivation, 2 cordyceps taishanensis secondary plant-expanding liquid cultivation and 3 cordyceps sinensis solid productive cultivation. The high-cordycepin-content rich-selenium cordyceps sinensis cultivation method is suitable for artificial cultivation of cordyceps sinensis and cordyceps militaris, obtained cordyceps sinensis is high in cordycepin content and selenium content, the cordycepin content can be 100-200 times of the cordycepin content of wild cordyceps sinensis, the selenium content can reach the selenium content level of the wild cordyceps sinensis, and the effective constituents and effect of the artificially-cultivated cordyceps sinensis are greatly improved. In addition, the high-cordycepin-content rich-selenium cordyceps sinensis cultivation method is high in sundry fungus resisting capacity and yield and has good economic benefit, and the artificial cultivation time can be shortened.