Culture medium and preparation method for cordyceps militaris fruiting body

Abstract

The invention relates to a culture medium for artificially cultivating Cordyceps militaris fruit bodies and a preparation method thereof. The ingredients used in the invention include wheat 85-90g, silkworm powder 3-4g, glucose 1-3g, peptone 1-3g, magnesium sulfate 0.1-0.2g, potassium dihydrogenphosphate 0.1-0.25g, triammonium citrate 0.1-0.25g, vitamin B1 0.01-0.03g, and purified water 40-50ml. the preparation method comprises the steps of dissolving the ingredients in water in the weight percentage, and then pouring the dissolved ingredients into a cultivation bottle; putting the wheat and the silkworm into the cultivation bottle according to weight percentage, sealing the cultivation bottle after stirring uniformly; and putting the sealed cultivation bottle in an electric sterilizer, and sterilizing for 3-4 hours on the condition of 121DEG C and 1.05MPa. The culture medium overcomes the disadvantages of low output wild aweto, too high production cost and lower biotransformation rate. The culture medium can ensure that the early development and growth of thalli is quick, the growth vigor of fruit bodies is uniform, the fruit bodies have deep color and are low in cost. Furthermore, through the culture medium, the output of main ingredient cordycepin is improved, and the output of cordycepic acid and cordycepin polysaccharide is also comparatively improved.

Description

technical field [0001] The invention relates to a culture medium, in particular to a culture medium for artificially cultivating fruiting bodies of Cordyceps militaris, belonging to the technical field of artificial cultivation of fungi. Background technique [0002] Cordyceps militaris (abbreviated as Cordyceps militaris), also known as Cordyceps militaris [C.militarise (L) Link], belongs to Ascomycotina (Asco mycotina) Sclerotinia (pyreno mycetes) Sphaerinels (Sphaerinels) Ergotaceae (claviticpitaceae) Cordyceps (Coedyceps). [0003] As the model species of the genus Cordyceps, Cordyceps militaris is a high-quality large fungus with both edible and medicinal value in my country. The research results of its chemical composition and pharmacological activity show that the fruiting body of Cordyceps militaris is rich in protein, amino acid, In addition to vitamins and trace elements such as calcium, manganese, zinc, selenium, etc., it also contains main active ingredients such...

AI Technical Summary

Problems solved by technology

[0005] Before the present invention, the current research on the cultivation of the fruiting body of Cordyceps militaris was still in the stage of small-scale cultivation in the laboratory, and did not enter the practical stage.

At the same time, most studies on the cultivation of Cordyceps militaris fruiting bodies use rice as the main component of the culture medium. Compared with other fungi, the production cost is too high, and the biotransformation rate is low, which is not economically meaningful.