Cordyceps sinensis fruiting body and cultivating method thereof

A technology for seeds and entities of Cordyceps sinensis, applied in the field of microorganisms, can solve the problems of the production, management and application of medicines and health care products, the influence of the medium composition and the culture conditions are not clear enough, the low yield is not conducive to wide application and the like.

Active Publication Date: 2014-03-26
YICHANG SHANCHENGSHUIDU CORDYCEPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned chaotic situation has had a great impact on the production, management and application of related drugs and health products
[0007] With the continuous deepening of research, there is clear evidence that the correct anamorph of Cordyceps sinensis is Mortierella sinensis [Hirsutella sinensis X.J.Liu, Y.L.Guo.], but there are only few reports that use Mortierella sinensis to cultivate Based on the content of cultivating the fruiting bodies of Cordyceps sinensis, such as the document "Research on Artificial Cultivation of Cordyceps Sinensis" (Yu Yongxin, Fungal Research, Volume 2, No. 2, 2004, pp. 42-46), but the composition of the medium and culture conditions in this document It is not clear enough, and the number of fruiting bodies is only 1 root / CFU, and the longest length is only about 5cm. The low yield is not conducive to wide application

Method used

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  • Cordyceps sinensis fruiting body and cultivating method thereof
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: The cultivation method of the present invention cultivates the fruiting body of Cordyceps sinensis

[0024] 1. Cultivation method

[0025] Weigh 3g of glucose, 1g of peptone, 0.5g of yeast extract powder, 0.5g of hydrolyzed milk protein, anhydrous MgSO 4 0.05g, KH 2 PO 4 0.1 g, 2 g of agar, and 100 ml of water were prepared into 4 test tubes with a diameter of 3 cm as solid medium and sterilized for future use. At the same time, it is isolated from wild Cordyceps sinensis, purified, and cultured to obtain the asexual type Trichosporum sinensis of Cordyceps sinensis.

[0026] Cut Mortierella sinensis into small pieces of 4×4 mm in size and connect them on each solid slant medium, and then put them into an environment of 18° C. and a relative humidity of 55% for 45 days.

[0027] Transfer the colony to an induction container with no light and an oxygen concentration of 12%. The relative humidity is controlled at 80%, the temperature is controlled at 5°...

Embodiment 2

[0030] Embodiment 2: ITS sequence analysis of Cordyceps sinensis fruiting body of the present invention

[0031] The fungal 18S, 5.8S, and 28S rDNA genes form a transcription unit, and the three are highly conserved. The spacer between them is the Internal Transcribed Spacer (ITS), and the fungal rDNA ITS sequence (Internal Transcribed Spacer) has an evolutionary Conservative, the detection of this sequence helps to analyze its genetic relationship.

[0032] Extract the DNA of the fruit body part of Example 1, perform ITS-specific PCR amplification, and perform sequencing and BLAST comparison in Genbank. The sequencing result is 586 bases. The sequence is shown in SEQ ID NO: 1, and the BLAST comparison result is Cordyceps sinensis [Cordyceps sinensis (BerK.) Sacc], see the BLAST comparison chart figure 1 .

Embodiment 3

[0033] Embodiment 3: The cultivation method of the present invention cultivates the fruiting bodies of Cordyceps sinensis

[0034] 1. Cultivation method

[0035] Weigh 2.67g of glucose, 1.00g of peptone, 0.50g of yeast extract powder, 0.50g of hydrolyzed milk protein, anhydrous MgSO 4 0.05g, KH 2 PO 4 0.10 g, 1.83 g of agar, and 100 ml of water were prepared into 4 test tubes with a diameter of 3 cm as solid medium and sterilized for future use. At the same time, it is isolated from wild Cordyceps sinensis, purified, and cultured to obtain the asexual type Trichosporum sinensis of Cordyceps sinensis.

[0036] Cut Mortierella sinensis into small pieces of 4×4 mm in size and connect them on a solid slant medium, and then culture them in an environment of 14°C and 45% relative humidity for 55 days.

[0037] Transfer the colony to an induction container with no light and an oxygen concentration of 15%. The relative humidity is controlled at 70%, the temperature is controlled...

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Abstract

The invention relates to the field of microorganisms, and discloses a cordyceps sinensis fruiting body and a cultivating method thereof. The cultivating method includes the steps that hirsutella sinensis is inoculated onto a solid culture medium, fungal colony cultivation is performed for 35-55 days under the conditions that sunlight is kept out, the temperature is at 14-20 DEG C and the relative humidity is 45-60%, and then the fruiting body is induced under the conditions that the sunlight is kept out, the oxygen concentration is 10-15%, the temperature is 0-17 DEG C and the relative humidity is 70-90% until the fruiting body grows. According to the method, the hirsutella sinensis is inoculated onto the specific culture medium, the conditions of humidity, temperature and the like at different growth stages of the hirsutella sinensis are controlled, the real cordyceps sinensis fruiting body is cultivated in the low-temperature, lucifugal and oxygen-poor environment, and the immunomodulatory effect and the active material content of the cordyceps sinensis fruiting body are almost the same as those of a wild cordyceps sinensis fruiting body.

Description

[0001] This application claims the priority of the Chinese patent application submitted to the China Patent Office on September 20, 2012, the application number is 201210356912.3, and the title of the invention is "a fruiting body of Cordyceps sinensis and its cultivation method", the entire content of which is incorporated herein by reference Applying. technical field [0002] The invention relates to the field of microorganisms, in particular to a fruiting body of Cordyceps sinensis and a cultivation method thereof. Background technique [0003] Cordyceps sinensis is a complex of fruiting bodies (fruiting bodies) and larvae grown by Cordyceps sinensis infected and parasitized on the larvae of bat moths. It is an edible fungus and is a traditional precious Chinese medicine in my country. It has various effects such as regulating immune system function, anti-tumor, and anti-fatigue. [0004] Bat moth larvae are infected by Cordyceps sinensis fungus (Cordyceps sinensis), the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 朱新燕梁关海梁蕾朱志钢李文佳谢俊杰蒋均才华献春
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS
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