Original ecological separation method of cordyceps sinensis hirsutella sinensis

A technology of Trichospora and Cordyceps sinensis in China, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems of growth of miscellaneous bacteria, inability to eliminate, difficult separation work, etc., and achieves short cultivation time and simple method. The effect of simple operation and disinfection methods

Inactive Publication Date: 2013-01-02
大化万福泉母婴饮品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large number of experiments have proved that when 75% alcohol is used for surface disinfection, a large number of miscellaneous bacteria will grow in 2 to 3 days, which cannot be eliminated at all, so that the growth of Trichospora chinensis is covered; even if the concentration of 0.1% mercuric chloride is used to After 10 minutes of surface disinfection, there are still a small amount of bacteria growing, which brings difficulties to the subsequent separation work

Method used

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  • Original ecological separation method of cordyceps sinensis hirsutella sinensis
  • Original ecological separation method of cordyceps sinensis hirsutella sinensis
  • Original ecological separation method of cordyceps sinensis hirsutella sinensis

Examples

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Embodiment 1

[0043] Source of material: Live host of Cordyceps sinensis as a natural culture medium, collected on November 5, 2011, from a state-owned ranch in Yushu County, Yushu District, Qinghai Province, 4000 meters above sea level; fresh Cordyceps sinensis as a source , On May 31, 2012, collected from a Tibetan ranch in Zhiduo County, Yushu District, Qinghai Province, in an alpine meadow at an altitude of 4,500 meters.

[0044] Chinese Coats sporangium spore isolation method:

[0045] 1. At the site of collecting fresh Cordyceps sinensis, choose one of the short fruit bodies (length 3cm), thick worm body (length 3.5cm, diameter 6.5mm), and you can see the white ascospores overflowing in the pregnant part of the ascus. Use it immediately. The kraft paper bag of the fungus wraps its fruiting body and puts it in the incubator;

[0046] 2. Transport the collected materials to the laboratory and immediately put them in the refrigerator at 0-4°C. After 5 days, take out the fresh cordyceps in the ...

Embodiment 2

[0055] Material source: same as Example 1

[0056] Chinese Coats sporangium spore isolation method:

[0057] 1. At the site of collecting fresh Cordyceps sinensis, choose one of the short fruit bodies (length 2.5cm), thick body (length 3.5cm, diameter 7mm), and you can see that the ascus is fuller, and use sterilized kraft paper bags Cover the fruit body and place it in an incubator;

[0058] 2. Transport the collected materials to the laboratory and immediately put them in the refrigerator at 0-4°C. Take out the fresh Cordyceps in the laboratory after 15 days. You can see that there are white filaments on the kraft paper, and the ascospores can be removed and used Use sterile scissors to cut the kraft paper with dense surface linear ascospores, and store them in sterilized PE boxes.

[0059] 3. In the laboratory, select 7 live larvae of 3.0cm Cordyceps sinensis host Hepidia, soak them in 75℃ alcohol for 3 minutes, and wash them with sterile water 3 times. Then cut off the head, out...

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Abstract

The invention discloses an original ecological separation method of cordyceps sinensis hirsutella sinensis. The original ecological separation method comprises a tissue separation method and an ascospore separation method. According to the method, seed sources are selected from fresh and ripening cordyceps sinensis in Yushu, Qinghai in China, and soil with the depth being 8 to 12 cm in a range with the diameter being 10 to 12cm around the sporocarp is remained during the collection; a culture medium is from living bodies of cordyceps sinensis host of swift moth larvae, white strong and living swift moth larvae in 4 to 5 age periods are selected, and sterile water is used for rinsing and storage and placing in time during the collection; the cordyceps sinensis and the living bodies of the cordyceps sinensis host of swift moth larvae are stored at the temperature being 0 to 4 DEG C for 0 to 15 days after the collection; the seed sources are transferred onto the culture medium for culture, and the parameters are controlled as follows during the culture: the temperature is 6 to 9 DEG C, the pH value is 6 to 6.5, the light illumination is 50 to 100lux, the air humidity is 55 to 60 percent, and the oxygen content is 20 percent.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method for the original ecological separation of the fungus of the cordyceps sinensis in the asexual stage—Horex chinensis. technical background [0002] Cordyceps is a rare treasure with the same medicine and food. It has been comparable to natural ginseng and deer antler for hundreds of years, and enjoys a high reputation at home and abroad. It is neither warm nor dry, especially suitable for the elderly, young, sick, weak, and deficient. It can be taken all year round and has the effects of nourishing the lungs and kidneys and rejuvenating children without side effects. However, due to its lack of natural resources and its price is more expensive than gold, various cordyceps have appeared in the market one after another, making it difficult to distinguish between true and false. [0003] In order to solve the contradiction between supply and demand, many scientists at home ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 马建彬
Owner 大化万福泉母婴饮品有限公司
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