30results about How to "Simple disinfection method" patented technology

The invention relates to an African daisy tissue culture seedling growing method which comprises the following steps: (1) explant selection and processing; (2) adventitious bud induction; (3) adventitious bud multiplication; (4) adventitious bud rooting; and (5) seedling hardening and acclimatization. The African daisy tissue culture seedling growing method has good sterilization effect, rooting time is short, growth time is fast, and seedling quality is high.

The invention discloses a culture method of peony embryonic callus as well as a culture medium. The peony embryonic callus is induced through adopting an MS culture medium which contains special components after modification (such as substances of 6-BA, KT cytokinin, TDZ plant growth regulators, PIC herbicide as well as casein hydrolysate); aiming at a peony embryo explant, the induction rate of the peony callus can achieve 100% by adopting the specific culture method provided by the invention, wherein the induction rate of embryonic callus can achieve 86% above.

The present invention discloses a disinfection method of bamboo embryo culturing, which takes healthy seed of bamboo in the same year as an explant, soaks the grain after shelling in medical alcohol for 4-6 min, and then stirs it in 0.1 wt. % mercuric chloride solution for 25-35 min or in 0. 2-0.3 wt. % sodium hypochlorite solution for 10-20min, at last drops it in medical alcohol for 4-6 min. The disinfection method is simple and can effectively remove surface bacilli and endogenetic bacilli, which lay the foundation of establishing sterilized system, promoting successive transfer culture and rooting, and provides technical characteristics for superior bamboo seed development and utilization.

The invention discloses a method for rapidly breeding miscanthus plant miscanthus giganteus embryo by tissue culture, comprising the following steps: A, selecting a young ear; B, carrying out primary induction, namely cutting the young ear into sections, sterilizing with alcohol, soaking with mercury chloride, then washing with sterile water, and inoculating the sections onto a primary induction culture medium of the young ear; C, carrying out subculture for seedling growth, namely carrying out successive transfer seedling growing on the obtained embryonic callus onto a subculture medium, carrying out subculture once, and transferring the embryonic callus onto a seedling growing culture medium, thus small young seedling is formed; D, carrying out root generating induction, namely inducting the young seedling on a root growing induction culture medium to generate a root, culturing, and transferring the young seedling onto a root growing culture medium, thus the young seedling differentiates white or yellow roots; and E, carrying out acclimatization and transplanting, namely transplanting the seedling into a flower pot filled with vermiculite and soil to be acclimatized, spraying acclimatization culture liquid and clear water onto the young seedling regularly, and transferring the seedling into a seedling nursery. The method is feasible and is simple to operate, the rapid breeding efficiency and the breeding coefficient of miscanthus giganteus are improved in short time, the appreciation is stable, the root system is robust, and the transplanting survival rate is high.

The invention discloses a culture medium and a culture method suitable for L.nutans Maxim anther and ovary, and belongs to the technical field of wild linen biology. The culture method comprises the steps of genotype selection, explant low-temperature treatment, explant surface disinfection, callus induction, callus differentiation, adventitious bud elongation and rooting culture. The anther and the unfertilized sperm house of the L.nutans Maxim are used as materials, a set of haploid regeneration plant culture system suitable for L.nutans Maxim is established, the haploid plant of L.nutans Maxim is directly obtained, certain reference values are provided for haploid breeding of the flax, and meanwhile, the method has certain significance in germplasm resource preservation and new varietybreeding of genetic improvement of flax, is a means for preserving wild genetic resources, and provides help for future scientific research work.

The invention belongs to the technical field of livestock and poultry epidemic prevention, and provides a disinfection method of pig farms. Cleaning is performed on the surrounding environment of a pig farm; comprehensive hydrogen-rich water disinfection, Chinese herbal medicine disinfection, silicon water disinfection and negative ion disinfection are carried out; and maintenance is performed after disinfection is finished. The disinfection method is simple and efficient and can effectively kill the viruses and bacteria in the pig farm, so that the repeated occurrence of epidemic diseases canbe prevented, the breeding costs of farmers can be reduced, and breeding earnings can be increased.

The invention discloses a method for quickly reproducing miscanthus plant miscanthus and triarrhena hybrid NO.9 somatic embryo tissue culture, which comprises the following steps of: (1) selecting young ears: selecting young ears of miscanthus and triarrhena hybrid NO.9 staying in the gramineae primordium formation period; (2) inducing the young ears by using a primary induction culture medium: cutting the young ears into small segments, disinfecting the segments with alcohol, soaking the segments in mercuric chloride, flushing the segments with sterile water, inoculating the segments on the primary induction culture medium of young ears, and inducing primary generation to obtain embryonal calluses; (3) cubculturing by using a seedling culture medium: subculturing the obtained embryonal calluses on a subculture medium; (4) inducing to grow roots by using a root growing induction culture medium: inducing the grown seedlings on the induction culture medium to grow roots; and (5) domesticating and transplanting: spraying a domesticating culture liquid and clean water to the seedlings regularly every day in a greenhouse, transplanting the seedlings to a nursery, and illuminating the seedlings to obtain the seedlings of miscanthus and triarrhena hybrid NO.9. The method is feasible and simple and convenient to operate, the differentiation callus induction rate and the qualified seedling percentage are high, the survival rate is high, and the method is applicable to quick reproduction in the industrial production of miscanthus and triarrhena hybrid NO.9 seedlings.

The invention relates to the field of drinking water disinfection, in particular to a tap water disinfection method. The method includes the specific steps that Ophicalcitum is smashed and evenly mixed with tap water to be treated and amylose, krypton is introduced into the tap water for sterilization, the tap water is inoculated with amylase in the proportion of 2% for preliminary enzymolysis, the treated tap water is fed into a clarification tank for clarification, pressure-maintaining clarification is conducted for 3-4 hours with the pressure in a clarification room being 30-35 MPa, and finally the disinfected tap water is obtained. The tap water disinfection method is very simple; substances harmful to the human body are not generated in the disinfection process, in other words, secondary pollution to water is avoided; common viruses (rotavirus, the hepatitis virus, enterovirus and the like) in water can be efficiently and thoroughly killed.