30results about How to "Simple disinfection method" patented technology

The present invention discloses a disinfection method of bamboo embryo culturing, which takes healthy seed of bamboo in the same year as an explant, soaks the grain after shelling in medical alcohol for 4-6 min, and then stirs it in 0.1 wt. % mercuric chloride solution for 25-35 min or in 0. 2-0.3 wt. % sodium hypochlorite solution for 10-20min, at last drops it in medical alcohol for 4-6 min. The disinfection method is simple and can effectively remove surface bacilli and endogenetic bacilli, which lay the foundation of establishing sterilized system, promoting successive transfer culture and rooting, and provides technical characteristics for superior bamboo seed development and utilization.

The invention discloses a method for quickly reproducing miscanthus plant miscanthus and triarrhena hybrid NO.9 somatic embryo tissue culture, which comprises the following steps of: (1) selecting young ears: selecting young ears of miscanthus and triarrhena hybrid NO.9 staying in the gramineae primordium formation period; (2) inducing the young ears by using a primary induction culture medium: cutting the young ears into small segments, disinfecting the segments with alcohol, soaking the segments in mercuric chloride, flushing the segments with sterile water, inoculating the segments on the primary induction culture medium of young ears, and inducing primary generation to obtain embryonal calluses; (3) cubculturing by using a seedling culture medium: subculturing the obtained embryonal calluses on a subculture medium; (4) inducing to grow roots by using a root growing induction culture medium: inducing the grown seedlings on the induction culture medium to grow roots; and (5) domesticating and transplanting: spraying a domesticating culture liquid and clean water to the seedlings regularly every day in a greenhouse, transplanting the seedlings to a nursery, and illuminating the seedlings to obtain the seedlings of miscanthus and triarrhena hybrid NO.9. The method is feasible and simple and convenient to operate, the differentiation callus induction rate and the qualified seedling percentage are high, the survival rate is high, and the method is applicable to quick reproduction in the industrial production of miscanthus and triarrhena hybrid NO.9 seedlings.

The invention discloses a method for tissue culture and rapid propagation of weeping tube flowers, which comprises the following steps: setting a culture medium; selecting and disinfecting explants to ensure that there are no defects in the outer scales; putting the scale buds after the disinfection treatment on the induction medium Carry out the induction culture of adventitious buds; the induced adventitious bud clusters are multiplied by adventitious buds, and the multiplied adventitious bud clusters are divided into individual plants under aseptic conditions and then transferred to the rooting medium for rooting induction culture; After the induction of rooting, the weeping tube flower plants are transplanted and hardened. The invention can obtain a large amount of high-quality and strong seedlings of the weeping tube flower with the same genetic character in a short period of time.

The invention discloses a high-efficiency multiplication method for virus-free gerbera seedlings, which comprises the following steps: 1) pretreatment of gerbera chrysanthemum virus-free seedlings; 2) induction of adventitious buds; 3) cutting adventitious buds for subculture; 4) Seedling hardening; 5) Hydroponic rooting treatment; 6) Hydroponic strong seedlings; 7) Transplanting. The high-efficiency multiplication method of the present invention solves the problems of low efficiency and easy re-pollution during the multiplication process of the virus-free gerbera seedlings. The problem of variety degeneration caused by chrysanthemum branch propagation and seed propagation is simple, easy to promote in factories, and has high practical value in production.