Method for rapidly breeding miscanthus plant miscanthus giganteus embryo by tissue culture

Abstract

The invention discloses a method for rapidly breeding miscanthus plant miscanthus giganteus embryo by tissue culture, comprising the following steps: A, selecting a young ear; B, carrying out primary induction, namely cutting the young ear into sections, sterilizing with alcohol, soaking with mercury chloride, then washing with sterile water, and inoculating the sections onto a primary induction culture medium of the young ear; C, carrying out subculture for seedling growth, namely carrying out successive transfer seedling growing on the obtained embryonic callus onto a subculture medium, carrying out subculture once, and transferring the embryonic callus onto a seedling growing culture medium, thus small young seedling is formed; D, carrying out root generating induction, namely inducting the young seedling on a root growing induction culture medium to generate a root, culturing, and transferring the young seedling onto a root growing culture medium, thus the young seedling differentiates white or yellow roots; and E, carrying out acclimatization and transplanting, namely transplanting the seedling into a flower pot filled with vermiculite and soil to be acclimatized, spraying acclimatization culture liquid and clear water onto the young seedling regularly, and transferring the seedling into a seedling nursery. The method is feasible and is simple to operate, the rapid breeding efficiency and the breeding coefficient of miscanthus giganteus are improved in short time, the appreciation is stable, the root system is robust, and the transplanting survival rate is high.

Description

technical field [0001] The present invention relates to Qigang ( Miscanthus giganteus ) technology field of tissue culture and rapid propagation based on embryogenic callus, and more specifically relates to a method for tissue culture and rapid propagation of somatic embryos of Miscanthus genus Miscanthus, which is suitable for obtaining Qigang young ears as explants through callus induction Seedlings, as well as multiplication, rooting, domestication and transplanting. Background technique [0002] Qigang ( Miscanthus x giganteus ) is a natural hybrid triploid (3n=57), which may be produced by crossing grass (2n=78) and awn (2n=38). Morphologically, this species is more similar to Grass, and its main feature is that the first lemma of the spikelets has no awn, and the stalks are erect. It's just that its rhizomes are more clustered, which is a transitional type between Di and awn. According to literature reports, the origin of Qigang may be Japan, but its natural ...

AI Technical Summary

Problems solved by technology

In the 1990s, there were relevant reports on the tissue culture and rapid propagation technology of Miscanthus in China. They used young leaves, hypocotyls, young roots, and young ears as explants for the rapid propagation of Miscanths. , but their callus rate and differentiation rate are different, the reproduction efficiency of the young panicle is the highest, but the article does not clearly point out that the obtained callus is embryogenic callus

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