Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of Uyghur drug Chama ancient polysaccharide and its extraction method and application

A technology for polysaccharide extraction and Uyghur medicine, which is applied in the field of plant effective component extraction and chamagu polysaccharide extraction. It can solve the problems of not being able to clarify the structure of the specific active ingredient, not knowing the approval of the active product, and failing to separate the active ingredient. , to achieve the effects of clear pharmacology, easier control of drug efficacy, and wide applicability

Active Publication Date: 2019-04-19
XINJIANG MEDICAL UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Patent CN201510011425.7 discloses a method of hot water extraction to prepare Xinjiang turnip polysaccharides with immunomodulatory activity, and CN201410266707.7 discloses an extract and preparation method of ancient polysaccharides of Chama Immunity and anti-fatigue activities, but the specific active ingredients have not been isolated, and the structure of the specific active ingredients has not been clarified, which limits the application of chamagu, especially in western countries, the recognition of medicinal materials with unclear active products is not high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of Uyghur drug Chama ancient polysaccharide and its extraction method and application
  • A kind of Uyghur drug Chama ancient polysaccharide and its extraction method and application
  • A kind of Uyghur drug Chama ancient polysaccharide and its extraction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: Preparation of Chama ancient polysaccharide of the present invention

[0053] In the present invention, the extraction of Chama ancient polysaccharide comprises the following steps:

[0054] (1) Take the dry medicinal material of Chamagu, add 3 times the volume of petroleum ether, reflux and degrease continuously at 60°C for 2 hours, filter the medicinal residue, dry it for later use, and recover the filtrate.

[0055] (2) Take the degreased Chama ancient medicinal material, add 30 times the quality of water, 90°C-100°C continuous reflux extraction for 3 times, each time for 2h, combine three extracts, after filtering, the filtrate is concentrated to 20% of the total amount, Add absolute ethanol until the volume fraction of the concentrated filtrate is 80%, and put it at 4°C overnight.

[0056] (3) Suction filtration step (2) Collect the precipitate after overnight solution, add 10-30 times of distilled water to redissolve, fully stir at room temperature ...

Embodiment 2

[0062] Embodiment 2: Preparation of Chama ancient polysaccharide of the present invention

[0063] In the present invention, in the extraction of Chama ancient polysaccharide, step (2) uses 90 DEG C of water to continuously reflux extraction 3 times; Step (3) uses the dialysis bag of 7000Da molecular weight; Said step (6) takes 500 parts of BRN, adds 0.03 times of pure water , fully dissolved at room temperature; other methods were the same as in Example 1, and the prepared BRNP-1 was prepared at a concentration of 400 μg·mL-1; the concentration of BRNP-2 was 400 μg·mL-1.

Embodiment 3

[0064] Example 3: Determination of the uniformity and apparent molecular weight of the ancient polysaccharide extracted from the present invention

[0065] Take 5mg of T-series dextran standard products (molecular weights are 2000, 670, 410, 270, 150, 80, 50, 12, 5 and 1kD), dissolve them fully in 1mL of ultrapure water, and prepare 5mg·mL-1 Dextran series standard solutions, passed through a 0.22μm filter membrane, set aside. Take 10 μL standard solution respectively, inject into high-performance gel chromatography (HPGPC), and use 0.1M NaNO 3 Elution, the flow rate is 0.6mL·min -1 , column temperature 35°C. Draw the standard curve with the peak area as the ordinate and the molecular weight as the abscissa.

[0066] Get 5 mg of the single-component sample of chama ancient polysaccharide extracted by the extraction method of the present invention, fully dissolve it with 1 mL of ultrapure water, and configure it into 5 mg·mL -1 The sample solution was passed through a 0.22 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an Uighur medicine brassica campestris polysaccharide as well as an extraction method and application thereof. The invention definitely discloses a structure formula of the brassica campestris polysaccharide and a method for preparing the brassica campestris polysaccharide. The method comprises the steps of degreasing petroleum ether, performing reflux extraction and performing separation and purification through an anion exchange column and a gel chromatography column. The prepared brassica campestris polysaccharide has an excellent immunoregulatory curative effect. In the prepared brassica campestris polysaccharide, the effective constituents of brassica campestris are more clear and definite; the preparation method is simple; the pharmacology is clearer; the dosage of the brassica campestris polysaccharide in the field of medicine is more accurate; the pharmacological effect is easily controlled; a basis is supplied for the synthesis of the brassica campestris polysaccharide; the brassica campestris polysaccharide has wide application in the fields of food and drugs.

Description

technical field [0001] The present invention mainly relates to the field of plant effective component extraction, specifically, the present invention relates to a technical field of chamagu polysaccharide extraction. Background technique [0002] Chama is also known as Zhugecai, Manjing, and Yuancaitou in ancient times. It is a subspecies of Brassicaceae Brassica turnip, and it is a favorite medicine and food homologous item for Uighur residents in Xinjiang. According to the Pharmacopoeia, its warm nature belongs to the stomach meridian, liver meridian, unblocks the triple burner, benefits the middle qi, sharpens the five internal organs, and detoxifies evil. It can nourish the lungs and relieve cough, clear the liver and improve eyesight, fill the essence and strengthen the kidneys, soften the intestines and laxatives, diuretic and reduce swelling, cure cholera, scrofula, breast cancer, and quench thirst. Qi, invigorating the stomach and eliminating food, detoxification a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/04
CPCA61K31/715A61K36/31C08B37/0003
Inventor 海力茜·陶尔大洪董彩霞陈卓尔热孜亚木·吾甫尔李金芳侯宝林古娜娜·对山别克阿依夏古丽·巴卡斯
Owner XINJIANG MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com