91 results about "Miscanthus" patented technology

One aspect of the invention relates to a process, comprising treating lignocellulosic biomass according to a first pretreatment protocol, thereby generating a first product mixture; separating the first product mixture into a first plurality of fractions; and treating at least one fraction of said first plurality of fractions according to a second pretreatment protocol, thereby generating a second product mixture. In one embodiment, the lignocellulosic biomass is selected from the group consisting of grass, switch grass, cord grass, rye grass, reed canary grass, miscanthus, sugar-processing residues, sugarcane bagasse, agricultural wastes, rice straw, rice hulls, barley straw, corn cobs, cereal straw, wheat straw, canola straw, oat straw, oat hulls, corn fiber, stover, soybean stover, corn stover, forestry wastes, recycled wood pulp protocol protocol fiber, paper sludge, sawdust, hardwood, softwood, and combinations thereof.

A process for converting lignocellulosic materials which are field residues such as cotton stalks and corn stover, process residues such as sugarcane bagasse and sweet sorghum bagasse, woody parts of energy crops such as switchgrass and miscanthus, forest residues or byproducts of the wood processing industries such as sawdust from sawmills to a liquid biofuel by a series of processing steps wherein the feed materials are hydrolysed in three stages and withdrawn as three product streams each consisting of solubilized fragments of one of the three major components of the feed materials and a set of concurrently operating processing steps wherein each of the three product streams is transformed through chemical or biochemical processes into products, such as pure lignin and ethanol, that have a high calorific value and process wherein these products with high calorific value are combined to form a liquid biofuel.

One aspect of the present invention relates to a method of processing lignocellulosic material, comprising initial steam pretreatment to give pretreated lignocellulosic material with an average particle size, followed by refining to give refined lignocellulosic material with an average particle size, wherein the average particle of the pretreated lignocellulosic material is greater than the average particle size of the refined lignocellulosic material. In certain embodiments, the lignocellulosic material is selected from the group consisting of grass, switch grass, cord grass, rye grass, reed canary grass, miscanthus, sugar-processing residues, sugar cane bagasse, agricultural wastes, rice straw, rice hulls, barley straw, corn cobs, cereal straw, wheat straw, canola straw, oat straw, oat hulls, corn fiber, stover, soybean stover, corn stover, forestry wastes, recycled wood pulp fiber, sawdust, hardwood, and softwood.

One aspect of the present invention relates to a method of processing lignocellulosic material, comprising initial steam pretreatment to give pretreated lignocellulosic material with an average particle size, followed by refining to give refined lignocellulosic material with an average particle size, wherein the average particle of the pretreated lignocellulosic material is greater than the average particle size of the refined lignocellulosic material. In ceratin embodiments, the lignocellulosic material is selected from the group consisting of grass, switch grass, cord grass, rye grass, reedcanary grass, miscanthus, sugar-processing residues, sugar cane bagasse, agricultural wastes, rice straw, rice hulls, barley straw, corn cobs, cereal straw, wheat straw, canola straw, oat straw, oat hulls, corn fiber, stover, soybean stover, corn stover, forestry wastes, recycled wood pulp fiber, sawdust, hardwood, and softwoo.

The invention discloses a preparation method and use of a simple sequence repeat (SSR) marker for screening miscanthus by a magnetic bead enrichment process, which comprises: 1) extracting genomic DNA of miscanthus floridulus which belongs to miscanthus; 2) incising the extracted total DNA by using restriction incision enzyme to obtain a genomic DNA; 3) intercrossing a probe and a target fragment; 4) enriching the hybrid DNA molecules having an SSR sequence by using magnetic beads; 5) amplifying and purifying the DNA fragment having the SSR sequence; 6) cloning and sequencing the DNA fragment; and 7) designing an SSR primer according to the flanking sequence of SSR, and designing a specific primer by using primer design software for amplifying the satellite fragment at the locus. The method is easy and convenient for operation; meanwhile, the SSR marker has the characteristics of high codominance, high polymorphism, multiple allele property and the like and can be used for analyzing the genetic diversity and genetic relationship of miscanthus and used in construction of genetic map of miscanthus, calibration of a target gene and drawing of a fingerprint.

The invention discloses a preparation method and application for a Miscanthus Genic-SSR mark. The method comprises the following steps: A, acquiring Miscanthus transcriptomic sequence, that is, selecting young and tender Miscanthus leaves, extracting total RNA respectively so as to obtain transcriptome Reads, removing the pollution of carrier RNA sequence and carrying out splicing so as to obtainthe Miscanthus transcriptomic sequence; B, identifying SSR sites in Miscanthus ESR sequence, that is, searching SSR sites in obtained non-redundant Unigene-EST sequence by using SSR retrieval software, with repeats being 2 to 6 bases, so as to obtain a series of Unigene sequences containing information about the SSR sites; C, designing Miscanthus Genic-SSR primers, that is, using primer design software to design primers according to the obtained Unigene sequences containing the SSR sites so as to obtain a series of the Miscanthus Genic-SSR primers. According to the invention, the method is simple, has high efficiency and is simple to operate; a huge amount of marks are obtained; EST sequence obtained through transcriptomic sequencing lays a foundation for genetic research, molecular improvement and the like of the critical energy plant Chinese silvergrass.

The invention discloses a method for preparing and using a miscanthus matrix for culturing agaricus bisporus. The matrix takes miscanthus as a main raw material. The method for preparing and using the miscanthus matrix comprises the following steps: grinding miscanthus, scattering urea, subsequently pre-humidifying, adding fresh chicken manure and dry cow dung, adding water, subsequently adding calcium superphosphate, gypsum and lime, piling for fermentation, performing pasteurization, fermenting again so as to obtain the matrix, and further seeding and earthing up. The method is simple in process, the nutrition proportioning is reasonable, the raw materials are easy to obtain, miscanthus is adopted to take the place of straw completely, great benefits and high economic values are achieved, the used raw materials are low in cost, that is, the cost of each square meter of the matrix is 10.5-11.5 yuan, the agaricus bisporus yield is high, and the mushroom fruiting amount is 16.0-17.5kg per square meter.

The invention provides a saline land plantation miscanthus method; the method uses seedling breeding to realize energy crop large scale production, thus greatly improving survival rate and rate of output, and providing high practicality and popularization property; the method comprises the following steps: saline land soil detection formula seedling growing; high furrow seedbed seedling growing; soil working and organic fertilizer applying before transplanting; irrigation salt restraining and laminating transplanting technical processing. The beneficial effects are that the method is reasonable and practical, saves time and labor, can realize energy crop large scale production, thus greatly improving survival rate, enterprise production efficiency and economic benefit, so the method is suitable for general popularization.

The invention relates to a method for preparing biological carbon used for capturing and storing carbon dioxide in the air from miscanthus, comprising the following steps: culturing the miscanthus, harvesting the miscanthus, preprocessing the miscanthus and producing the biological carbon. In the step of processing the miscanthus, the stalks of the harvested miscanthus are air-dried and cut into segments with certain length so as to be suitable for smokeless coking process, and then the stalks of the processed miscanthus are produced into the biological carbon at high temperature of 400-700 DEG C in the absence of oxygen by the smokeless coking process. The biological carbon storage methods comprise a centralized biological carbon storage method and an indirect biological carbon storage method. The biological carbon can be used for soil reclamation or as strategic reserve energy. The biological carbon can be used for economically and efficiently capturing and storing the carbon dioxide in the air without causing pollution to environment.

The invention belongs to the technical field of biological sectioning and relates to a method for sectioning and dyeing miscanthus plants. The method comprises the following steps: taking 3cm-10cm of a fresh miscanthus plant stem; immersing by alcohol and carrying out suitable softening treatment; sectioning and dyeing a sectioned thin sheet; fixing and de-coloring; then dyeing and flaking microscopic examination. According to the method, the softening degree is easy to master and the problems that the sheet is too hard or too soft and the like are solved; the sectioning integrity is easy to realize; the method is good for post-period dyeing and flaking.

The invention discloses a cultivation method for ganoderma lucidum. Th cultivation method comprises four technical links of slant culture, shake-flask culture, fermentation tank culture and bacteria stick culture, wherein each technical link comprises formula, culture medium preparation and a culture method. Particularly, the formula of a bacteria stick culture medium is prepared from 10-16 percent of acacia rachii wood particles, 7-12 percent of cyclobalanopsis tree and / or birch wood particles, 4-8 percent of miscanthus grass powder, 3-5 percent of corn flour, 3-5 percent of wheat flour, 1.5-2.5 percent of soybean meal, 1.4-2.0 percent of wheat bran, 0.6-0.9 percent of saccharose, 0.6-0.9 percent of lime and the balance of water. Compared with the prior method, the whole cultivation method is simple and easy for scale operation; the yield of the ganoderma lucidum is improved by about 30 percent while the cost is reduced to the maximum extent.

The invention discloses a method for identifying the genetic relationship by developing an SNP molecular marker of miscanthus with the SLAF-seq technology. The method comprises the following steps: (1) designing a marker development scheme; (2) selecting an optimal digestion scheme; (3) respectively extracting DNA of genomes for digesting, constructing an SLAF-seq sequencing library, and sequencing; (4) developing an SLAF marker for a single sample, then performing similarity clustering on all SLAF markers to obtain an SLAF marker of the cluster; (5) performing allele number, genetic sequence difference and other polymorphic analysis on the cluster SLAF marker, wherein SNP is taken as a polymorphic marker; (6) coding the genotype for parent, representing a character by a genotype, detecting the daughter genotype after the separation type is determined, and representing the SNP coding genotype with a corresponding character; and (7) selecting SNP marker with few sample loss, extracting all SNP sites, calculating the daughter SNP anomalous typing ratio, determining the genetic relationship between daughter and parent according to whether the daughter SNP anomalous typing ratio is higher than the threshold value or not.

The invention relates to a chemical disinfection tissue culture method for miscanthus giganteus. The method comprises the following steps: culturing container disinfection, medium preparation, callus induction culturing, differentiation culturing, rooting culturing, and bottle seedling transplanting. According to the method, chemical reagents are used for achieving the aim of bacteria killing or bacteria inhibition; in the process of preparing culture media, culture containers and the culture media do not need to be subjected to high-temperature and high-pressure disinfection, so that the work amount and the energy consumption can be reduced, and the step of miscanthus giganteus tissue culture is simplified; the operation is easy as the media are prepared according to different media formulas; the practicability is high, the generalization performance is high, and the tissue culture cost of miscanthus giganteus can be effectively reduced and can be reduced by 10% or more generally.

The invention discloses a method for in-situ restoration of a saline-alkali land ecosystem in the Yellow River delta by using triarrhena lutarioriparia and biochar, and belongs to the technical fieldof saline-alkali land improvement. According to the addition of the biochar, specifically, calculation is conducted according to the soil pH, the salt content and the soil property, the soil pH of coastal salt-alkali land of the Yellow River delta is 8.5, the salt content is 0.75%, the soil is sandy clay loam in property, the content of organic matter is low, and the application amount of the biochar is 2-4% of the weight of the soil. An application method comprises the steps that (1) the land is leveled; (2) the specific addition amount of the biochar is calculated according to the soil density and planting depth of an improvement site, the specific addition amount of the biochar is 2-4% of the weight of the soil, and a calculation method for the weight of the soil is that the weight of the soil is equal to a product of the surface area of the improvement site, the planting depth and the soil density; (3) a calculated amount of biochar is evenly laid on the surface of the soil, and soil turning and even mixing are conducted; (4) aging is conducted for three days after even mixing, and miscanthus of triarrhena lutarioriparia can be planted. By means of the technical scheme, the in-situ restoration of the saline-alkali land ecosystem in a coastal area of the Yellow River delta can be achieved, physiochemical characteristics and biocenosis structures of the soil of the restored saline-alkali land are greatly changed, and the restored saline-alkali land is suitable for the survival and normal growth of miscanthus.

The invention provides a method for producing a medium-density fiberboard through branches and trunk of ricinus communis and stalks of miscanthus. The method comprises the following steps: 1, after the branches and trunk of ricinus communis and stalks of miscanthus are mixed, cutting the mixture into pieces; 2, humidifying and preheating the pieces, and performing fiber separation through a defibrator; 3, applying urea resin, an ammonium chloride curing agent and a paraffin waterproofing agent after fiber mixing, and uniformly mixing; 4, drying the fiber mixture; 5, screening the dried fibers, and manufacturing the fiber rough board through hot pressing of a hot press; 6, cooling the rough board, cutting edges and sanding, so as to obtain the finished product medium-density fiberboard. The medium-density fiberboard manufactured through the method provided by the invention is smooth in surface, uniform in fiber texture, moderate in density, good in dimensional stability, good in machining performance, high in bonding strength, static bending strength and plane tensile strength, good in elastic modulus, strong in bolt holding force, and low in moisture absorption and water absorption performance. According to the method, the branches and trunk of ricinus communis and stalks of miscanthus are used to produce the fiberboard, that branches and trunk of ricinus communis are cut down in successive years for updating is achieved and the forest fire caused by stalks of miscanthus is eliminated, and the method is suitable for areas, where ricinus communis and miscanthus grow.

The invention belongs to the technical field of plant genetic engineering and particularly relates to an MIWRKY12 gene obtained from Miscanthus by separating and cloning and relevant application of the MIWRKY12 gene in parenchymal cell incrassation and blooming of plants. A nucleotide sequence of the MIWRKY12 gene is represented by SEQ ID No.1, and an amino acid sequence of the MIWRKY12 gene is represented by SEQ ID No.2. According to the application, a feasible method is provided for increasing the biomass of the plants and regulating and controlling the flowering phase of the plants.

A continuous process for production of cellulose pulp from grass-like plant feedstock for paper making. The process includes: preparing the grass-like plant feedstock by comminuting, dedusting, continuous digestion, disperging, diluting the cellulose pulp, screening and fractionation, concentration to remove black liquor, dilution with fresh water, and, optionally, pulp bleaching processes. The continuous digestion is performed in the vertical column at 70-100° C. for 40 minutes to 2 hours; average composition of thus formed digestion suspension is maintained within the following ranges: 0.9-1.5% w / w of NaOH; 0.15-0.4% w / w of NaCl or Na2SO3; and 15-18% w / w of grass-like plant feedstock; concentrations of ingredients being calculated on the weight of the liquid phase. Screening and fractionation are resulting with two fractions; one being further processed by milling, and another being further processed to final pulp with or without bleaching processes. The preferred grass-like feedstock for the process is miscanthus / Miscanthus×giganteus, Andersson / .

The invention provides mechanical operation equipment for miscanthus plants. An engine is mounted at the front end of a frame and a handrail frame is mounted at the rear end of the frame; the engine is connected with a driving system and a rotary tillage mechanism, which are mounted on the frame, through a transmission system; a seedling cutting and conveying mechanism comprises a seedling cutting mechanism and a seedling conveying mechanism; the driving system is connected with the seedling conveying mechanism; a seedling disc is mounted on the frame and is connected with the seedling cutting mechanism; a planting mechanism is arranged under the seedling conveying mechanism; meanwhile, a height limited wheel assembly and a rear bracket are arranged under the handrail frame; and a seat is mounted on the rear bracket. The mechanical operation equipment integrates double functions of rotary tillage and planting and is hitched by adopting a chassis of a self-walking type tractor, and the power is small; and in a rotary tillage process, seedling guide type planting work is carried out, and a planting mechanism for guaranteeing planting perpendicularity of miscanthus seedlings in a high-speed planting process can be omitted; the mechanical operation equipment has a compact structure and is convenient and practical; the planting efficiency is improved effectively, an application range is wide and the adaptability is strong; and the working efficiency is greatly improved and large-scale planting of miscanthus energy plants is facilitated.

The invention discloses a method for rapidly breeding miscanthus plant miscanthus giganteus embryo by tissue culture, comprising the following steps: A, selecting a young ear; B, carrying out primary induction, namely cutting the young ear into sections, sterilizing with alcohol, soaking with mercury chloride, then washing with sterile water, and inoculating the sections onto a primary induction culture medium of the young ear; C, carrying out subculture for seedling growth, namely carrying out successive transfer seedling growing on the obtained embryonic callus onto a subculture medium, carrying out subculture once, and transferring the embryonic callus onto a seedling growing culture medium, thus small young seedling is formed; D, carrying out root generating induction, namely inducting the young seedling on a root growing induction culture medium to generate a root, culturing, and transferring the young seedling onto a root growing culture medium, thus the young seedling differentiates white or yellow roots; and E, carrying out acclimatization and transplanting, namely transplanting the seedling into a flower pot filled with vermiculite and soil to be acclimatized, spraying acclimatization culture liquid and clear water onto the young seedling regularly, and transferring the seedling into a seedling nursery. The method is feasible and is simple to operate, the rapid breeding efficiency and the breeding coefficient of miscanthus giganteus are improved in short time, the appreciation is stable, the root system is robust, and the transplanting survival rate is high.

A tissue paper web composed of one or more layers. At least one layer includes pulp fibers originating from at least one plant belonging to the genus Miscanthus wherein the pulp fibers are selected from chemical pulp fibers, mechanical pulp fibers subjected to a chemical pretreatment and mixtures thereof. The use of Miscanthus pulp fibers can lead to a major absolute increase in tensile strength along with good softness values.

The invention discloses a method for measuring the dry matter of Miscanthus leaves, which comprises the following steps in sequence: 1) collecting the Miscanths leaves to be measured; Internal scanning, thereby obtaining the reflected light spectrum at the following 23 characteristic wavelengths; 3), the data obtained in the above step 2) is substituted into the dry matter content calculation formula, thereby obtaining the dry matter content of the Miscanthus leaves to be measured. By adopting the invention, the dry matter content of the Miscanth plant leaf can be detected rapidly without damage, so as to speed up the measurement speed and reduce the measurement cost.

A method and apparatus for processing harvested miscanthus or other rhizomes to condition them for replanting to grow miscanthus plants Quantities of clusters of rhizomes are supplied to a first inclined ramp conveyor, the clusters of rhizomes are caught on a mechanism that pushes the clusters of rhizomes to a location where the clusters of rhizomes encounter obstructers that cooperate with the mechanism to cut and reduce the size of the clusters. The reduced size clusters are vibrated to loosen and separate soil from the reduced size cluster of rhizomes, washed to wash dirt from the clusters, and collected for supply to a planter.

The invention relates to the biomass energy field, and especially relates to a Miscanthus spp. plant energy potential evaluating method comprising the following steps: selecting property indexes, and measuring Miscanthus spp. Plant property index data; obtaining property index scores; obtaining the energy potential index according to the property index scores. The research method can systematically breed the Miscanthus spp. energy plant; the method uses simple and direct agronomic, quality and stress resistance differences to build the Miscanthus spp. plant energy potential evaluating system; the system method is simple in operation, can only test the plants so as to fast determine germplasm energy potential and best utilization approach, thus laying basis for Miscanthus spp. energy plant breeding and large scale Miscanthus spp. plant germplasm energy potential evaluation work.

The present invention provides varieties of fertile Miscanthus that have greater water deficit tolerance, greater vigor, greater cold tolerance, later flowering, and / or higher biomass, i.e., greater biomass than a control Miscanthus plant, and methods for producing and using the said Miscanthus varieties. These varieties may be used to produce cellulosic biofuels, or to produce inbred or hybrid Miscanthus plants. Plant cells, seeds and other plant parts are also described.

The invention relates to the field of plant propagation, and mainly provides a method for large-scale asexual propagation of field Chinese silvergrass through stem cutting at the Yellow River Delta region in autumn. The method comprises the following steps of land selection and land preparation, selection and treatment of cutting slips, placement of seedling raising paper cylinders, cutting of thecutting slips, earthing, watering, secondary earthing and film covering. The method has the advantages that (1) Chinese silvergrass propagation can be carried out at the Yellow River Delta region inautumn; (2) the labor can be reduced, the land is saved, and the energy sources are saved; (3) the Chinese silvergrass propagation coefficient is greatly increased, the large-scale and standardized propagation is performed, and a propagation method is provided for the large-scale and standardized production of Miscanthus in the Yellow River Delta saline-alkali area.

The invention relates to a comprehensive optimization treatment technology for saline-alkali soil, which is characterized in that the following measures are applied to saline-alkali soil to reduce the salt content of the soil: scarifying surface soil, paving a far infrared mineral base material, blending organic fertilizer, planting oil miscanthus of halophyte, harvesting to remove the salt content, and finally detecting the salt content and pH value of the soil. The far infrared mineral base material can generate shockwaves which can miniaturize water molecules so as to accelerate the salt absorption benefit of the oil awns, and meanwhile, the far infrared mineral base material has physical, chemical, biological and fertility properties and can be combined with organic fertilizer to promote the growth of the oil awns; the oil miscanthus crops can adsorb return salt of the oil miscanthus when the oil miscanthus is evaporated on the soil surface so as to dilute salt in the soil and underground water, the harvested oil miscanthus can supplement insufficient nutrition of the rice and the wheat, and the oil miscanthus straws can be used as feed and can be eaten by seeds, so that the oil miscanthus has great economic value; therefore, the far infrared mineral base material and the oil miscanthus are used and supplement each other, so that the soil of the saline-alkali soil can be effectively improved, and meanwhile, the benefit of soil optimization can be increased by virtue of the economical efficiency of the oil miscanthus.

The invention discloses a telescopic water pipe. The telescopic water pipe comprises an elastic inner pipe for allowing liquid to pass through and a weaving layer, wherein the elastic inner pipe has a shrinkage length and an extending length under the action of the pressure of the liquid passing through the elastic inner pipe; the weaving layer is wrapped around the outer circumferential surface of the elastic inner pipe; and the elastic inner pipe and the weaving layer form a first telescopic pipe body jointly, the first telescopic pipe body stretches and retracts along with stretching and retracting of the elastic inner pipe, and the outer diameter of the elastic inner pipe under the retracting length and the stretching length is close to the outer diameter of the elastic inner pipe under the corresponding length. Thus, the weaving layer protects the elastic inner pipe from being damaged by external sandstones and miscanthus, the weaving layer can be synchronously stretches and retracts along with the elastic inner pipe, the elastic inner pipe cannot be ground, and the service life of the first telescopic pipe body is prolonged; and due to the fact that the weaving layer is woven on the elastic inner pipe in a gapless mode, the use amount of the weaving layer is saved, the prepared first telescopic pipe body is small in size, small in the occupied space and light in the weight, and the first telescopic pipe body with unlimited length can be prepared.

The invention relates to a miscanthus anderss plant feed processing preparation method. Miscanthus anderss feed is prepared by virtue of steps of selecting materials, adjusting moisture, chopping, kneading, preparing with a leavening agent, mixing, anaerobically treating and preparing biological feed. The miscanthus anderss plant feed processing preparation method has the beneficial effects that the difficulty that miscanthus anderss leaf edges and leaf sheaths are stiff and the palatability is poor are overcome; during fermentation, a contact area is increased, the fermentation efficiency is increased, the microorganism fermentation activity can be improved, and the problems of short storage time, low utilization rate and poor palatability of fresh miscanthus anderss feed can be solved; an operation process of the method is simple; treated miscanthus anderss has the advantages of good palatability, juiciness, storage endurance, improved nutrition and the like; and the prepared miscanthus anderss biological feed has special acidic taste, has the characteristics of favorite for ruminant animals, rich nutrients, juiciness and storage endurance, can achieve an annual utilization effect, has a good application effect and facilitates the popularization.