175results about How to "Easy to grow on a large scale" patented technology

The invention discloses a method for cultivating pure white needle mushrooms. The method comprises the following steps: a strain is prepared; a culture material is prepared; bagging and sterilization are carried out; inoculation is carried out; a mycelium is cultured; nutrition, humidity, temperature, light, air and other culture conditions are strictly controlled during a fruiting period; and harvesting is carried out in an optimum period. the formulation of the culture material is 0 to 32 percent of weed tree sawdust, 0 to 93 percent of cotton seed hulls or wheat straw, straw, corncob and other crop straw, 0 to 20 percent of wheat bran, 0 to 10 percent of corn meal, 0 to 10 percent of soybean meal, 0 to 1 percent of calcium carbonate, 0 to 0.2 percent of monopotassium phosphate, 0 to 0.2 percent of magnesium sulfate, 1 to 1.2 percent of sucrose, 0 to 10 percent of rice bran, 0 to 1 percent of plaster and 0 to 0.02 percent of urea. Quicklime and wettable carbendazim are added during the preparation of the culture material so as to prevent the pollution of undesired bacteria. The technical proposal aims to select proper breeds, popularize local large scale planting and improve cultivation benefit.

The present invention provides a recombinant fusion protein which stimulates the rejuvenation and reactivation of skin and epidermal cells for improving skin appearance, smoothing wrinkles and freckles, and whitening skin. Particularly, the present invention provides various types of products for improving skin, which contain recombinant fusion protein of human serum albumin (HSA) with cytokine peptides (EGF, FGF, KGF, HGH, HGF, PDGF, GCSF, interferon, IL-11 or IGF) by genetic engineering technology. The fusion protein can be used independently or in a combination or combination with yeast fermentation products, or with varied emulsifiers, thickeners, moisturizer, preservatives, yeasts and ferments.

An interleukin-15 (IL-15) gene modified natural killing cell strain for immunotherapy of tumor is prepared through inserting the cDNA coding region of IL-15 in pcDNA3 eucaryotic expression carrier, configuring secretion-type recombinant eucaryotic expression carrier pc DNA3 / IL-15, using liposom to transfecte cell NK-92, adding Geneticin (G418) to screening culture medium, screening, limited dilution to cloned cells, detecting activity, choosing positive cell clones, and amplification.

A method for cultivating Bacillus licheniformis being applied in BOD biological transducer includes separating and screening waste water from starch plant for obtaining a high breath-strength aerobic bacterial strain, acclimating the bacterial strain to be operational bacterial strain set in BOD biological transducer for in dustrial waste water or seawater.

The invention relates to a method for planting apocynum venetum in an arid area salinification desert. In the method, trickle irrigation facilities are overlaid in the salinification soil environment, hollow billet trickle irrigation zones are distributed in the mode of one tube in one row; apocynum venetum seeds are planted on two sides of the hollow billet in a strip cropping mode; straws are covered in the sowing zone, and water is dripped in time to reduce evaporation loss and save water resource; the water-saving irrigation technology is utilized to create the low-salt environment for apocynum venetum seeds to bud and young seedlings to grow; and the young seedlings are built. According to the method disclosed by the invention, inhibition on pocynum venetum seed germination and young seedling growth is overcome, the complex process of transplanting the pocynum venetum seedling raising is overcome, the pocynum venetum can be planted on a large scale, and therefore the technical reserve is accumulated for developing and utilizing large-area saline-alkali soil wasteland in the arid area.

The invention provides a method for preparing (3S)-3-(tertbutyloxycarbonyl)amino-1-chlorin-4-phenyl-(2R)-butanol by microbial transformation, comprising the following steps of: using (3S)-3-(tertbutyloxycarbonyl)amino-1-chlorin-4-phenyl-2-butanone as a substrate, using enzyme-containing somatic cells obtained by fermentation of saccharomyces cerevisiae (Saccharomycescerevisiae)CGMCCNo.2266 as a biocatalyst, and performing a conversion reaction to obtain the product. The strain produced by the invention is safe and nontoxic; and the microbial thallus is easy for large-scale culture and requires lower cost than a chemical catalyst and katalaze. In addition, the method provided by the invention requires mild reaction condition, is environmentally friendly, has high molar conversion rate, is easy to realize large-scale industrial production, and is a green technology for the industrial production of (3S)-3-(tertbutyloxycarbonyl)amino-1-chlorin-4-phenyl-(2R)-butanol.

The invention discloses a compound cultivation method of pinellia ternata. The method adopts the stereoscopic interplanting mode of three plants: interplanting fructus forsythiae belonging to shrub below high mangnolia officinalis belonging to arbor; and interplanting the pinellia ternata below the fructus forsythiae. As the method is used, the production cost is lowered; the ecological environment is protected; the quality of the pinellia ternata is improved; and the economic benefit is increased. The pinellia ternata is interplanted with the mangnolia officinalis and the fructus forsythiae, so that the degradation of the planted pinellia ternata is avoided; the production cost is lowered by 30%; and the residues of organic substances in soil are reduced. Therefore, the method is favorable for the large-scale planting of the pinellia ternata.

The invention relates to the field of bioengineering, in particular to a culture medium and a preparation method thereof. According to a formula, an agaricus bisporus liquid spawn includes raw materials and a water solution. The raw materials are contained in the water solution and are composed of the following components by weight: 0.1-1.0% of yeast powder, 1.5-3% of sugar, 0.05-0.3% of potassium dihydrogen phosphate, 0.05-0.3% of magnesium sulfate, and 0.1-0.5% of peptone. The preparation method of the agaricus bisporus liquid spawn comprises the steps of: culture medium preparation, culture medium adjustment, culture medium sterilization, and mycelium inoculation. By adopting the technology, the agaricus bisporus mycelia cultivated by the invention have the advantages of vigorous vitality, difficult aging, rapid spawn running speed, and strong disease resistance, thus being easy for large-scale planting.

The invention discloses a method for planting pure white needle mushrooms with chicken dung as a culture material. The planting method comprises the following steps: a strain is prepared; chicken dung is treated; the culture material is prepared; bottling and sterilization are carried out; inoculation is carried out; a mycelium is cultured; nutrition, humidity, temperature, light, air and other culture conditions are strictly controlled during a fruiting period; and the chicken dung which is accumulated for fermentation, spread out, sun-dried and ground is added to the culture material, so as to provide protein, mineral matter, carbohydrate and a plurality of nutritional ingredients needed by the growth of needle mushrooms. The formulation of the culture material is 78 to 93 percent of cotton seed hulls, 1 to 1.2 percent of sucrose, 0.5 to 1 percent of plaster, 1 to 1.2 percent of sucrose, 0 to 15 percent of chicken dung and 0 to 10 percent of wheat bran. A certain amount of quicklime is added during the preparation of the culture material, so as to avoid the pollution of undesired bacteria, diseases and insect pests and guarantee the edible safety of the needle mushrooms. The technical proposal has the advantages of promoting the accelerated growth of edible fungi, raising yield and shortening growing time.

The invention discloses a fast reproduction method for sessiie siemona root tissue culture. The fast reproduction method includes steps of obtaining sessiie siemona root sterile stems with buds, inducing axillary buds, subculturing cluster buds, inducing rooting, and the like. According to the fast reproduction method for sessiie siemona root tissue culture, tissue culture is performed by using a tissue culture method, genetic stability is good, a growth period is short, and a large number of sessiie siemona root seedlings can be obtained.

The invention discloses a method for cultivating Taiwan pleurotus geesteranus in the north of Anhui province. The method comprises the following steps of (1) strain selection; (2) culture medium preparation, wherein the culture materials comprises 85 percent of organic matter cultivation material aggregated diamond nanorod core and 15 percent of wheat bran, the inorganic additives comprise 1 percent of gypsum and 2-4 percent of lime, the moisture content of the culture medium is 55-65 percent, the pH value is adjusted to be 6-8, and bagging and sterilizing are carried out; (3) inoculation; (4) spawn running: cultivating the culture medium inoculated with pleurotus geesteranus strains for 20-25 days at the temperature of 20-32 DEG C and the relative air humidity of 80-90 percent, so that hyphae are grown all over in the bag; (5) fruiting: opening the hypha plastic bag, keeping the environment temperature to be 10-25 DEG C and the relative air humidity to be 85-95 percent, spraying water for 2-3 times and ventilating for 1-2 times every day, providing scattered light with the light intensity of 300-500Lx, so the pleurotus geesteranus grow; (6) harvesting and shifting to moisture management. The pleurotus geesteranus produced by the method has the characteristics of high biological efficiency, rich nutrient and the like, moisture mushrooms can be harvested continuously for 2-3 times, and the yield is high.

The invention relates to a growth method for a ZnO nanotube array. The method includes the steps that under the alkaline condition, the ZnO nanotube array is prepared through a hydrothermal synthesis method with a Zn piece as a substrate and a reaction source; and according to the formation of the ZnO nanotube array, ZnO nanometer pieces are firstly formed on the substrate and then grow into nanometer sticks, the centers of the nanometer sticks are eroded, and finally, the nanometer sticks are eroded into nanotubes. The hydrothermal method is low in growth temperature and cost, easy to operate and beneficial to large-scale growth; the controllable growth of the ZnO nanotube array can be successfully achieved by only regulating the growth time; and hope is brought to the low-cost large-scale production technology meeting the development requirement of novel devices and industries, and an effective backup method is provided for the size-controllable growth of other nanometer materials.

This invention provides a method for culture bacteria that can reduce high concentration Cr (VI) in basic medium. The bacteria are colorless Achromobacter sp. (CGMCC 1260) with a preservation name of Ch-1. this invention adopts nitrogen and carbon sources as the culture media, and culture at 25-25 deg.C, pH value of 7-11, and Cr (VI) concentration of 1-2000 mg / L. this invention can raise Cr (VI) reduction capacity to higher than 2g / L, and can be used in chemical plants or chromium salt treatment plants for large scale Cr (VI) reduction.

The invention discloses a seedling raising method for summer squash. The seedling raising method comprises the following concrete steps: disinfection treatment, seed soaking treatment, microwave treatment, ultrasonic treatment and ultraviolet treatment. The method provided by the invention is scientific, reasonable, simple and practicable, is simple and convenient to manage, and facilitates operation implementation by growers; transplanted plants have strong drought resistance; and the planted summer squash has the advantages of fresh and tender quality, good taste, high nutritional value, applicability to large-scale cultivation, high yield, good quality, and capability of completely meeting the needs of market.

The invention discloses a rapid propagation method for asparagus fern mutagenesis breeding. The rapid propagation method comprises the following steps: seed disinfection, seed mutagenesis, cluster bud proliferation, rooting induction and the like. According to the method disclosed by the invention, metabolic pathways of cells are changed by processing through a mutagenic reagent so as to obtain high-yield strains with genetic stability.

The invention discloses a recombinant yeast Kluyveromyces for expressing antibodies or antibody analogues and application thereof. The invention provides a method for constructing the recombinant yeast Kluyveromyces for expressign antibodies or antibody analogues. The method comprises the following steps: encoding genes of the antibodies or the antibody analogues are guided into the yeast Kluyveromyces to obtain recombinant bacteria; the antibody analogues are fusion proteins formed by Fc fragments of the antibodies and proteins or protein subunits; the encoding genes of the antibodies are heavy chain and light chain encoding genes of the antibodies. The recombinant yeast Kluyveromyces for expressing antibodies or antibody analogues can be cultured to produce complete antibodies or antibody analogues. The invention uses the yeast Kluyveromyces to prepare the antibodies or antibody analogues to preliminarily overcome the difficulties of difficult antibody production, high purity and large quantity requirements for products and provide good application prospects.

The invention discloses a rapid propagation method for smilax glabra roxb tissue culture. The rapid propagation method for smilax glabra roxb tissue culture comprises the steps of obtaining smilax glabra roxb sterile blades, inducing callus, differentiating callus, inducing rooting, acclimatizing and transplanting, and the like. With the adoption of the tissue culture method, smilax glabra roxb seedlings can be obtained in a large batch, the production is controllable, the quality is excellent, and a basis is provided for development and utilization of smilax glabra roxb.

The invention discloses a method for planting dendrobium officinale. The method comprises the following steps that A, a greenhouse is erected, the top of the greenhouse is covered with a plastic film and a shade net, a seedbed is arranged in the greenhouse, and peat soil, peanut shells, pine bark and limestone powder raw materials are powdered to serve as a matrix in the seedbed; B, sprouts are prepared, dendrobium officinale seedlings are selected as a seedling source of tissue culture seeds, and 3-4 seedlings in each cluster are evenly planted in the matrix; C, watering is carried out; D, fertilizing is carried out; E, harvesting is carried out; F, storage is carried out. According to the method, the survival rate of the dendrobium officinale can be increased, cultivation time is saved, economic benefits are increased, and large-scale planting is promoted.

The invention discloses a club fungus cultivating method and a product thereof. The club fungus cultivating method comprises the steps that basal cell division is promoted while auricularia polytricha sporocarp development is interrupted by adjusting and controlling temperature and light intensity and cooperating with proper high-concentration CO2, top end cells of auricularia polytricha bases are simulated to continue to divide and growth upwards when normal cell division in auricularia polytricha basal bodies is up to a certain degree, so that the auricularia polytricha bases undergo the expanding, growing and proliferating process and bifurcate from the base portions, the middle-upper portions bifurcate for multiple times to form clusters, the top ends form finger-shaped cluster sets, finally coral grape vine shapes are formed, and the appearance, taste and nutritive value of club fungi are superior to those of club fungi of auricularia polytricha.

The invention discloses a field intensive planting method of Polygonatum kingianum Coll.et Hemsl. The field intensive planting method comprises two steps of seedling-raising and intensive planting, wherein the seedling-raising method adopts seed seedling-raising or rhizome seedling-raising, and the method of seed seedling-raising comprises the following steps: S1, seed selection and treatment; S2,preparation of a seedling-raising substrate; S3, nursery design; S4, nursery disinfection; S5, sowing; and S6, nursery management. In the field intensive planting method, the seedling-raising substrate is combined with a plant growth regulator, a seedling-raising cycle of Polygonatum kingianum Coll.et Hemsl is greatly shortened, and the field intensive planting method realizes large-scale planting of Polygonatum kingianum Coll.et Hemsl, and reduces the labor cost of large-scale planting of Polygonatum kingianum Coll.et Hemsl.

The invention belongs to the technical field of planting set agriculture planting and particularly relates to a strawberry planting method for fast propagation and fruiting in summer. Before soil is frozen in the last ten days of November, strong strawberry seedlings with flower buds fully differentiated are excavated, the roots of the strong strawberry seedlings are placed into a root protection solution to be soaked for 12-14 hours, the strong strawberry seedlings are placed to a shady and cool ventilating place for being aired for 6-7 hours, in May of a next year, the strong strawberry seedlings are taken out from a refrigeration room, the roots are placed into a nutrient solution to be soaked for 2-3 hours, meanwhile, soothing serum is sprayed to leaf faces, a 600-fold carbendazim solution is used for sterilizing the roots of the strong strawberry seedlings, fixed plantation is conducted after 17:00 in afternoon, certain light shielding is conducted after fixed plantation when the intensity of illumination is larger than 108 lux, thorough watering is conducted once every other five days after fixed plantation except the rainy days, after strawberries fruit, a monosaccharide solution is sprayed on the surfaces of fruits every other 10 days, the survival rate of the strong strawberry seedlings processed through the strawberry planting method is larger than 98 percent, after fixed plantation, strawberry fruits can be harvested for about 40-50 days, the cultivation time is shortened, the yield per mu can reach 1800-2200 kilograms, and the economic benefits are obvious.

The invention relates to a method for constructing and planting ceratoideslatens (J.F.Gmel.) revealet holmgren in desert grassland of an arid region. The method comprises the steps of seed collection and storage, land selection and preparation, sowing and management at the seedling stage. Water retaining ridges are arranged in sloping fields among mounds of the desert grassland, ceratoideslatens (J.F.Gmel.) revealet holmgren seeds are mixed with soddy soil, the mixture is stirred to be uniform, the seeds are scattered on the sloping field which is 20 cm in front of each ridge, and the method follows emergence rules of seedlings of ceratoideslatens (J.F.Gmel.) revealet holmgren to create the appropriate soil environment for germination of the ceratoideslatens (J.F.Gmel.) revealet holmgren seeds and growth of the seedlings, so that artificial construction and plantation of the seedlings are achieved. By means of the method, the defects that germination of the ceratoideslatens (J.F.Gmel.) revealet holmgren seeds and growth of the seedlings are inhibited by drought and water shortage, and transplantation of the ceratoideslatens (J.F.Gmel.) revealet holmgren seedlings is complex are overcome, on-site construction and plantation of ceratoideslatens (J.F.Gmel.) revealet holmgren are achieved, and furthermore, technical reserve is accumulated for fostering improvement of the desert grassland of the arid region.

The invention discloses a carrier-free immobilized culture method of a microbial flocculating agent. The method is characterized by comprising the following steps of: adding self-flocculating bacteria spore suspension in a volume which is 0.1 to 1 percent of the volume of a culture medium into the culture medium, wherein each millimeter of suspension contains 106 self-flocculating bacteria spores; putting the culture medium into a shaking bed, and performing culture and fermentation, wherein the culture conditions comprise that the revolution speed is 150 to 180 revolutions per minute, the temperature is 20 to 30 DEG C and the pH is 3.5 to 10.5; and standing after the suspension is cultured for 24 to 120 hours, and separating mycelium pellets generated by the self-flocculating bacteria and the fermentation solution, wherein the solid part is the mycelium pellets, and the liquid part is the microbial flocculating agent. Sucrose is used as a carbon source, sodium nitrate is used as a nitrogen source, the self-flocculating bacteria generate stress reaction by using the characteristic that the self-flocculating bacteria can form the mycelium pellets and improving the salt concentration of the culture medium, and the purpose of generating the microbial flocculating agent by the self-flocculating bacteria is fulfilled. The microbial flocculating agent prepared by the method has the advantages of low cost, extensive production conditions and high flocculating activity, and can be used for treating multiple kinds of printing and dyeing wastewater.

The invention discloses a rapid propagation method for suspension cell culture for savatier monochasma herb, and the rapid propagation method comprises the steps as follows: obtaining the sterile blades, inducing the callus tissue and cultivating suspension cells; the savatier monochasma herb suspension culture growth rate is high and the growth is stable, and the rapid propagation method for suspension cell culture for savatier monochasma herb is the much more direct route for developing and using medicinal resources.

The invention provides a culture medium, culture method and application of avian pasteurella multocida. In the culture method, the culture medium is simple and clear in composition, can be used for culturing the avian pasteurella multocida, can meet carbon sources and nitrogen sources required by microbial growth and is especially suitable for quick growth of the avian pasteurella multocida, large-scale culturing of the avian pasteurella multocida is facilitated, and the culture period is shortened. Meanwhile, adjuvants such as animal serum or whole blood do not need to be added into the culture medium, the cultured avian pasteurella multocida facilitates preparation of vaccines, and the vaccines have the characteristics that high stability is achieved, animal stress response is not proneto being caused, and the protection rate is high.

The invention discloses recombinant yeast for expressing an antibody or an antibody analogue as well as a construction method and application thereof. In the construction method of the recombinant yeast for expressing the antibody or the antibody analogue, an encoding gene of the antibody or an encoding gene of the antibody analogue is introduced in a yeast to obtain a recombinant bacterium; the antibody analogue is a fusion protein formed by an Fc fragment of the antibody and a protein or a protein subunit; the encoding gene of the antibody is a heavy chain encoding gene and a light chain encoding gene of the antibody, and the 5' end of the encoding gene of the antibody or the encoding gene of the antibody analogue is connected with an encoding signal peptide nucleotide sequence; and signal peptide is serum albumin signal peptide. By utilizing the recombinant yeast for expressing the antibody or the antibody analogue to prepare the antibody and the antibody analogue, the invention can solve the bottlenecks of difficult antibody production, requirements of high product purity and large amount and the like and can also exert the functions provided by the antibody Fc fragment, thereby having a favorable application potential.

The invention relates to a method for cultivating selenium-enriched pepper through selenium-enriched nutritive cubes. The method is characterized by comprising the following steps that pepper seeds are soaked into a sodium selenite solution with the concentration of 0.03% and placed into an incubator under the temperature of 18-20 DEG C for germination acceleration, after germination, the seeds are sown in the nutritive cubes containing 400 mg / kg or above sodium selenite, 1-3 seeds are sown in each cub, the seeds together with the nutritive cubes are transplanted into a land for growing field crops after seedling formation, and management is performed according to a conventional method; the selenium-enriched nutritive cubes become a selenium source of the overall pepper growth period, planting can be performed in a selenium-enriched region and a selenium-deficient region, the selenium content of the grown selenium-enriched pepper is 0.2-1.42 mg / kg. The method is simple and easy to implement, low in cost, good in effect and capable of remarkably improving the taste of pepper and promotes large-scale production, and the selenium content of the produced selenium-enriched pepper is consistent.

The invention discloses a method for effectively expressing sTNFR / Fc fusion protein in yeast and the application thereof, which belongs to the medical field. The method for preparing tumor necrosis factor soluble receptor and antibody IgG Fc fragment fusion protein by the yeast comprises: (1) culturing the yeast including the gene used for encoding the fusion protein; (2) purifying and preparing the fusion protein from the culture. The invention has the advantage that the invention discloses a method for effectively expressing sTNFR / Fc fusion protein in the yeast and a purifying method thereof. The yeast is unicellular inferior eukaryote, which not only has the characteristics of being easy to culture prokaryote, low culturing cost, rapid propagation, convenient large-scale culture, high-density fermentation, and the like, but also has the sugar chain processing system of eukaryote; furthermore, the yeast can secrete foreign protein to culture fluid, which is beneficial to purification. Therefore, the method and the application thereof have favorable application prospect.

The invention relates to a rhizoma arisaematis cultivating and planting method which comprises: selecting seedlings with a seedling height of 6cm to 9cm from a seedling supply station, implanting plantlets with slight mud pies, which grow healthily and strongly, into a medicinal liquid tank, transplanting the plantlets to a field according to a planting distance of 20cm*15cm after one to two days, and then completely watering for once; and covering roots of rhizoma arisaematis with medicine mud, coating exposed positions of roots of rhizoma atractylodis with the medicine mud, and finally, carrying out seedling bed management. The rhizoma arisaematis cultivating and planting method has the advantages that the cultivating method provided by the invention is strong in seed viability, low in soil property requirements and high adaptability, is beneficial to large-scale plantation, and is high in later medicinal value. By experiments, when the method is utilized to cultivate the seedlings, investment cost of soil per mu is reduced by two sevenths in a later period, a survival rate reaches over 80 percent, and cultivated seedlings suffer from diseases and insect pests a little and have long service lives.

The invention discloses a bamboo cutting planting method, which comprises the steps of soil treatment, cutting branch treatment, cutting, field management and the like. The bamboo cutting planting method provided by the invention can use bamboo branches to directly carry out cutting planting, which changes the bamboo planting mode, reduces the bamboo planting cost, and greatly improves the survival rate of the cutting planting at the same time, which is beneficial to large-scale planting of bamboo.