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Fast reproduction method for sessiie siemona root tissue culture

A technique of erecting one hundred branches and tissue culture, which is applied in the field of plant cultivation to achieve the effects of less energy consumption, favorable for large-scale planting and large output

Inactive Publication Date: 2014-12-24
NANJING DIDAO AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is used for chronic cough, lung fatigue cough, whooping cough; it is used externally for head lice, body lice, ecdysis, and pruritus vulvae. There is no research report on artificial cultivation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the young shoots of upright Baibu, remove the excess leaves, soak in detergent for 5 minutes, rinse with running water for 1 hour, treat with 75% alcohol on the ultra-clean workbench for 40 seconds, treat with 10% hydrogen peroxide for 10 minutes, rinse with sterile water 5 times, and disinfect The treated erect stems with buds were inserted into GD+ZT0.25mg / L+NAA0.001mg / L+6-BA0.25mg / L+0.7% agar+45g / L sucrose medium to induce axillary buds , pH5.82, light intensity 2000lx, photoperiod 12h / d, temperature 25°C, put the induced axillary buds into GD+ZT0.1mg / L+NAA0.001mg / L+6-BA0.1mg / L+0.7% agar +45g / L sucrose medium for subculture of clustered buds, add 45g / L sucrose, 7g / L agar, pH5.82, light 3500lx, temperature 25°C, put the erect clustered shoots after subculture into Keep the humidity at about 90% in the medium containing fine sand, properly shade, keep ventilation, and increase light. After one month, the rooting rate is counted, and the rooting rate is 91%.

Embodiment 2

[0011] Take the young shoots of upright Baibu, remove the excess leaves, soak in detergent for 5 minutes, rinse with running water for 1 hour, treat with 75% alcohol on the ultra-clean workbench for 40 seconds, treat with 10% hydrogen peroxide for 10 minutes, rinse with sterile water 5 times, and disinfect The treated erect stems with buds were inserted into GD+ZT0.25mg / L+NAA0.001mg / L+6-BA0.25mg / L+0.7% agar+45g / L sucrose medium to induce axillary buds , pH5.82, light intensity 2000lx, photoperiod 12h / d, temperature 25°C, put the induced axillary buds into GD+ZT0.25mg / L+NAA0.002mg / L+6-BA0.25mg / L+0.7% agar +45g / L sucrose medium for subculture of clustered buds, add 45g / L sucrose, 7g / L agar, pH5.82, light 3500lx, temperature 25°C, put the erect clustered shoots after subculture into Keep the humidity at about 90% in the medium containing fine sand, properly shade, keep ventilation, increase light, and count the rooting rate after 1 month, and the rooting rate is 90%.

Embodiment 3

[0013] Take the young shoots of upright Baibu, remove the excess leaves, soak in detergent for 5 minutes, rinse with running water for 1 hour, treat with 75% alcohol on the ultra-clean workbench for 40 seconds, treat with 10% hydrogen peroxide for 10 minutes, rinse with sterile water 5 times, and disinfect The treated erect stems with buds were inserted into GD+ZT0.25mg / L+NAA0.001mg / L+6-BA0.25mg / L+0.7% agar+45g / L sucrose medium to induce axillary buds , pH5.82, light intensity 2000lx, photoperiod 12h / d, temperature 25°C, put the induced axillary buds into GD+ZT0.25mg / L+NAA0.001mg / L+6-BA0.25mg / L+0.7% agar +45g / L sucrose medium for subculture of clustered buds, add 45g / L sucrose, agar 7g / L, pH5.82, light 3500lx, temperature 25°C, put the erect clustered shoots after subculture into Keep the humidity at about 90% in the medium containing fine sand, properly shade, keep ventilation, and increase light. After one month, the rooting rate is counted, and the rooting rate is 93%.

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PUM

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Abstract

The invention discloses a fast reproduction method for sessiie siemona root tissue culture. The fast reproduction method includes steps of obtaining sessiie siemona root sterile stems with buds, inducing axillary buds, subculturing cluster buds, inducing rooting, and the like. According to the fast reproduction method for sessiie siemona root tissue culture, tissue culture is performed by using a tissue culture method, genetic stability is good, a growth period is short, and a large number of sessiie siemona root seedlings can be obtained.

Description

technical field [0001] The invention relates to a rapid propagation method for erect plant culture and belongs to the technical field of plant cultivation. Background technique [0002] upright hundred, Stemonase silifolia , also known as drug lice medicine, a nest of tigers, Libudaceae, erect Libu is a perennial herb, semi-shrub, growing in hillside shrubs or under bamboo forests. Mainly produced in Anhui, Jiangsu, Hubei, Zhejiang, Shandong. It is suitable for landscaping, as an ornamental ground cover, or as a plant in a flower border. The roots are excavated in spring and autumn, removed the fibrous roots, washed, put in boiling water for a while or steamed until there is no white heart, taken out, and dried in the sun for medicinal purposes. It likes a warm and humid climate, preferably with deep, fertile sandy loam or humus-rich loam. It is native to the mountainous areas of Zhejiang, Jiangsu, Anhui, Shandong, and Henan provinces. Chemical constituents: Containing ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘东锋杨成东
Owner NANJING DIDAO AGRI SCI & TECH
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