Fast reproduction method for sessiie siemona root tissue culture

A technique of erecting one hundred branches and tissue culture, which is applied in the field of plant cultivation to achieve the effects of less energy consumption, favorable for large-scale planting and large output

Inactive Publication Date: 2014-12-24
NANJING DIDAO AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is used for chronic cough, lung fatigue cough, whooping cough; it is used externally for head li

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0008] Example 1

[0009] Take the young shoots that stand upright, remove the extra leaves, soak in detergent for 5 minutes, rinse in running water for 1 hour, treat with 75% alcohol on an ultra-clean workbench for 40 seconds, treat with 10% hydrogen peroxide for 10 minutes, rinse with sterile water 5 times, and disinfect The treated stems with buds of upright stomata are connected to GD+ZT0.25mg / L+NAA0.001mg / L+6-BA0.25mg / L+0.7% agar+45g / L sucrose medium to induce axillary buds , PH5.82, light intensity 2000lx, light period 12h / d, temperature 25℃, the induced axillary buds are put into GD+ZT0.1mg / L+NAA0.001mg / L+6-BA0.1mg / L+0.7% agar Subculture of cluster buds in +45g / L sucrose medium, add sucrose 45g / L, agar 7g / L, pH 5.82, light 3500lx, temperature 25°C, put the subcultured upright stalk buds into In the medium containing fine sand, keep the humidity around 90%, properly shade, maintain ventilation, and increase the light. After 1 month, the rooting rate is counted, and the roo...

Example Embodiment

[0010] Example 2

[0011] Take the young shoots that stand upright, remove the extra leaves, soak in detergent for 5 minutes, rinse in running water for 1 hour, treat with 75% alcohol on an ultra-clean workbench for 40 seconds, treat with 10% hydrogen peroxide for 10 minutes, rinse with sterile water 5 times, and disinfect The treated stems with buds of upright stomata are connected to GD+ZT0.25mg / L+NAA0.001mg / L+6-BA0.25mg / L+0.7% agar+45g / L sucrose medium to induce axillary buds , PH5.82, light intensity 2000lx, light period 12h / d, temperature 25℃, the induced axillary buds are put into GD+ZT0.25mg / L+NAA0.002mg / L+6-BA0.25mg / L+0.7% agar Subculture of cluster buds in +45g / L sucrose medium, add sucrose 45g / L, agar 7g / L, pH 5.82, light 3500lx, temperature 25°C, put the subcultured upright stalk buds into In the medium containing fine sand, keep the humidity at about 90%, properly shade, maintain ventilation, and increase the light. After 1 month, the rooting rate is calculated, and ...

Example Embodiment

[0012] Example 3

[0013] Take the young shoots that stand upright, remove the extra leaves, soak in detergent for 5 minutes, rinse in running water for 1 hour, treat with 75% alcohol on an ultra-clean workbench for 40 seconds, treat with 10% hydrogen peroxide for 10 minutes, rinse with sterile water 5 times, and disinfect The treated stems with buds of upright stomata are connected to GD+ZT0.25mg / L+NAA0.001mg / L+6-BA0.25mg / L+0.7% agar+45g / L sucrose medium to induce axillary buds , PH5.82, light intensity 2000lx, light period 12h / d, temperature 25℃, the induced axillary buds are put into GD+ZT0.25mg / L+NAA0.001mg / L+6-BA0.25mg / L+0.7% agar Subculture of cluster buds in +45g / L sucrose medium, add sucrose 45g / L, agar 7g / L, pH 5.82, light 3500lx, temperature 25°C, put the subcultured upright stalk buds into The medium containing fine sand should maintain a humidity of about 90%, properly shade, maintain ventilation, and increase light. After 1 month, the rooting rate was counted, and t...

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PUM

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Abstract

The invention discloses a fast reproduction method for sessiie siemona root tissue culture. The fast reproduction method includes steps of obtaining sessiie siemona root sterile stems with buds, inducing axillary buds, subculturing cluster buds, inducing rooting, and the like. According to the fast reproduction method for sessiie siemona root tissue culture, tissue culture is performed by using a tissue culture method, genetic stability is good, a growth period is short, and a large number of sessiie siemona root seedlings can be obtained.

Description

technical field [0001] The invention relates to a rapid propagation method for erect plant culture and belongs to the technical field of plant cultivation. Background technique [0002] upright hundred, Stemonase silifolia , also known as drug lice medicine, a nest of tigers, Libudaceae, erect Libu is a perennial herb, semi-shrub, growing in hillside shrubs or under bamboo forests. Mainly produced in Anhui, Jiangsu, Hubei, Zhejiang, Shandong. It is suitable for landscaping, as an ornamental ground cover, or as a plant in a flower border. The roots are excavated in spring and autumn, removed the fibrous roots, washed, put in boiling water for a while or steamed until there is no white heart, taken out, and dried in the sun for medicinal purposes. It likes a warm and humid climate, preferably with deep, fertile sandy loam or humus-rich loam. It is native to the mountainous areas of Zhejiang, Jiangsu, Anhui, Shandong, and Henan provinces. Chemical constituents: Containing ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘东锋杨成东
Owner NANJING DIDAO AGRI SCI & TECH
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