Fast reproduction method for sessiie siemona root tissue culture
A technique of erecting one hundred branches and tissue culture, which is applied in the field of plant cultivation to achieve the effects of less energy consumption, favorable for large-scale planting and large output
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[0008] Example 1
[0009] Take the young shoots that stand upright, remove the extra leaves, soak in detergent for 5 minutes, rinse in running water for 1 hour, treat with 75% alcohol on an ultra-clean workbench for 40 seconds, treat with 10% hydrogen peroxide for 10 minutes, rinse with sterile water 5 times, and disinfect The treated stems with buds of upright stomata are connected to GD+ZT0.25mg / L+NAA0.001mg / L+6-BA0.25mg / L+0.7% agar+45g / L sucrose medium to induce axillary buds , PH5.82, light intensity 2000lx, light period 12h / d, temperature 25℃, the induced axillary buds are put into GD+ZT0.1mg / L+NAA0.001mg / L+6-BA0.1mg / L+0.7% agar Subculture of cluster buds in +45g / L sucrose medium, add sucrose 45g / L, agar 7g / L, pH 5.82, light 3500lx, temperature 25°C, put the subcultured upright stalk buds into In the medium containing fine sand, keep the humidity around 90%, properly shade, maintain ventilation, and increase the light. After 1 month, the rooting rate is counted, and the roo...
Example Embodiment
[0010] Example 2
[0011] Take the young shoots that stand upright, remove the extra leaves, soak in detergent for 5 minutes, rinse in running water for 1 hour, treat with 75% alcohol on an ultra-clean workbench for 40 seconds, treat with 10% hydrogen peroxide for 10 minutes, rinse with sterile water 5 times, and disinfect The treated stems with buds of upright stomata are connected to GD+ZT0.25mg / L+NAA0.001mg / L+6-BA0.25mg / L+0.7% agar+45g / L sucrose medium to induce axillary buds , PH5.82, light intensity 2000lx, light period 12h / d, temperature 25℃, the induced axillary buds are put into GD+ZT0.25mg / L+NAA0.002mg / L+6-BA0.25mg / L+0.7% agar Subculture of cluster buds in +45g / L sucrose medium, add sucrose 45g / L, agar 7g / L, pH 5.82, light 3500lx, temperature 25°C, put the subcultured upright stalk buds into In the medium containing fine sand, keep the humidity at about 90%, properly shade, maintain ventilation, and increase the light. After 1 month, the rooting rate is calculated, and ...
Example Embodiment
[0012] Example 3
[0013] Take the young shoots that stand upright, remove the extra leaves, soak in detergent for 5 minutes, rinse in running water for 1 hour, treat with 75% alcohol on an ultra-clean workbench for 40 seconds, treat with 10% hydrogen peroxide for 10 minutes, rinse with sterile water 5 times, and disinfect The treated stems with buds of upright stomata are connected to GD+ZT0.25mg / L+NAA0.001mg / L+6-BA0.25mg / L+0.7% agar+45g / L sucrose medium to induce axillary buds , PH5.82, light intensity 2000lx, light period 12h / d, temperature 25℃, the induced axillary buds are put into GD+ZT0.25mg / L+NAA0.001mg / L+6-BA0.25mg / L+0.7% agar Subculture of cluster buds in +45g / L sucrose medium, add sucrose 45g / L, agar 7g / L, pH 5.82, light 3500lx, temperature 25°C, put the subcultured upright stalk buds into The medium containing fine sand should maintain a humidity of about 90%, properly shade, maintain ventilation, and increase light. After 1 month, the rooting rate was counted, and t...
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