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Camellia chrysantha tissue culture propagation method

A Camellia japonica and tissue culture technology, applied in the field of plant propagation, can solve the problems of less research on the tissue culture of Camellia japonica, and achieve the effects of significant multiplication effect, overcoming a large number of materials, and high reproduction coefficient

Active Publication Date: 2015-01-21
南宁市金花茶公园
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In previous reports, there are relatively few studies on the tissue culture of Camellia japonica, especially in terms of rooting induced by tissue culture plantlets of Camellia japonica.

Method used

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  • Camellia chrysantha tissue culture propagation method
  • Camellia chrysantha tissue culture propagation method
  • Camellia chrysantha tissue culture propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The basic medium used in this method includes MS and MW medium. MS basic medium formula: KNO 3 1900mg / L, NH 4 NO 3 1650mg / L, CaCl 2 2H 2 O 440mg / L, MgSO 4 ·7H 2 O 370mg / L, KH 2 PO 4 170mg / L, KI 0.83mg / L, H 3 BO 3 6.2mg / L, MnSO 4 4H 2 O 22.3mg / L, ZnSO 4 ·7H 2 O 8.6mg / L, Na 2 Mo 4 ·H 2 O 0.025mg / L, CuSO 4 ·5H 2 O 0.025mg / L, CoCl 2 ·6H 2 O 0.025mg / L, FeSO4 ·7H 2 O 27.8mg / L, Na 2 .EDTA·7H 2 O 37.3mg / L, vitamin B1 1mg / L, vitamin B6 1mg / L, niacin 1mg / L, inositol 20mg / L, glycine 2mg / L, sucrose 2-4%, agar 6-7g / L. MW medium formula: except for the content of macroelements, other elements are the same as MS medium, and the content of macroelements is as follows: KNO 3 80mg / L, Ca(NO 3 ) 2 4H 2 O 150mg / L, MgSO 4 ·7H 2 O 350mg / L, NaH 2 PO 4 ·H 2 O 100mg / L, KCl 65mg / L, Na 2 SO 4 200mg / L.

[0032] The mature common camellia camellia seeds collected in October 2013 were used as explants, and the seed coat was peeled off. Before inoculation, the ...

Embodiment 2

[0040] The basic medium MS and MW medium used in this method are the same as in Example 1.

[0041] The mature seeds of Fusui Dongdong Golden Camellia collected in October 2013 were used as explants, and the seed coat was peeled off. Before inoculation, the surface was sterilized with 75% alcohol for 40 seconds, and then sterilized with 8% NaClO solution for 25 minutes. , and finally rinse with sterile distilled water on the ultra-clean bench until it is clean.

[0042] Cut the sterilized seeds in the middle, and put the part with the embryo into the primary induction medium. The first-generation induction basic medium is based on MS medium, which also includes 6-BA 0.5mg / L, NAA 0.2mg / L, sucrose 30g / L and agar 6g / L, and the pH is adjusted to 5.8 to induce sterile bud.

[0043] The aseptic buds induced by the first generation were cut into terminal buds and stems with buds and inoculated on the subculture medium respectively. The subculture medium used MS as the basic me...

Embodiment 3

[0049] The basic medium MS and MW medium used in this method are the same as in Example 1.

[0050] The hybrid golden camellia seeds collected in October 2013 were used as explants, and the seed coat was peeled off. Before inoculation, the surface was sterilized with alcohol with a volume concentration of 75% for 60 seconds, and then sterilized with a NaClO solution with a volume concentration of 5% for 30 minutes. Rinse with sterile distilled water until clean.

[0051] Cut the sterilized seeds in the middle, and put the part with the embryo into the primary induction medium. The first-generation induction basic medium is based on MS medium, which also includes 6-BA 0.2mg / L, NAA 0.5mg / L, sucrose 30g / L and agar 6g / L, and the pH is adjusted to 5.8 to induce sterile bud.

[0052] The aseptic buds induced by the first generation were cut into terminal buds and stems with buds and inoculated on the subculture medium respectively. The subculture medium used MS as the basic m...

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Abstract

The invention provides a camellia chrysantha tissue culture propagation method. The camellia chrysantha tissue culture propagation method comprises the steps of obtaining aseptic seedlings, propagating the aseptic seedlings, culturing strong multiple shoots, expanding propagation and carrying out rooting culture, wherein camellia chrysantha embryos are selected as explants and then inoculated to a primary culture medium to obtain the aseptic seedlings of the camellia chrysantha; next, the aseptic seedlings are inoculated to a subculture multiplication culture medium and a strong shoot culture medium; the seedling propagation and strengthening processes are repeated and circulated until a large quantity of strong aseptic seedlings are obtained; finally, the aseptic seedlings are transplanted to a rooting culture medium for rooting culture. The camellia chrysantha tissue culture propagation method has the advantages that the vitrification problem of the aseptic seedlings in the camellia chrysantha tissue culture process is overcome, multiple subculture of materials can be realized, large-scale propagation is realized, the seedling growing period of the camellia chrysantha is shortened, the seedling growing materials are saved, the rooting rate is 95%-98%, and the transplanting survival rate is more than 90%; as a result, the camellia chrysantha tissue culture propagation method has excellent economic benefit, social benefit and ecologic benefit.

Description

technical field [0001] The invention belongs to the technical field of plant propagation and relates to tissue culture seedling raising technology, in particular to a method for tissue culture propagation of camellia japonica. Background technique [0002] In 1960, Chinese scientists first discovered a golden camellia in Nanning, Guangxi, which was named Camellia japonica. The discovery of camellia camellia has caused a sensation in the global horticultural circles and the press, and has been highly valued by horticulturalists at home and abroad. It is considered to be the best raw material for cultivating golden camellia varieties. Camellia japonica is an ancient plant, extremely rare. It is as famous as precious "living fossils of plants" such as silver fir, spinosa, and Davidia involucrata. Clan Queen". In addition, golden camellia contains special genetic genes for color and luster, which has important scientific research value. According to scientific identification,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李桂娥李志辉罗燕英黄晓娜蒋昌杰黄连冬文萍叶品明
Owner 南宁市金花茶公园
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