Tissue culture method of gynura divaricata

Abstract

The invention discloses a tissue culture method of gynura divaricata. The method comprises the steps of: inductive culture: inoculating on MS start medium without any hormone to induce generation of axillary bud; multiplication culture: inoculating on the medium MS+6BA, 0.2 mg / L+sucrose 20 g / L+ of agar 6 g / L for multiplication; and rooting culture: rooting on MS+IBA 0.1 mg / L+sucrose 20 g / L+agar 6 g / L, or MS+IBA 0.3 mg / L+IAA 0.3 mg / L+sucrose 20 g / L+agar 6 g / L, wherein the culture temperature is 25+ / -1 DEG C, the illumination intensity is 20,001x, and the illumination time is 16 h / d. The method disclosed by the invention can quickly and efficiently produce healthy and uniform high-quality gynura divaricata seedlings with pure characteristics at large scale.

Description

technical field

[0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for tissue culture of Panax notoginseng. Background technique

[0002] White-backed Sanqi (Gynura divaricata (L.) DC.) belongs to the genus Panax notoginseng of the Compositae family, also known as Baidongfeng, Baizicao, Yuloquat, Sanbaibang, Thick Noodles, Chicken Vegetables, Big Fat Beef, White Saffron, White Red cabbage, Fugui cabbage, Baizi cabbage, etc. are distributed from Taiwan to South China and Southwest China. Its roots, stems and leaves can be used as medicine; its roots are sweet and cool in nature, can clear away heat and cool blood, dissipate blood stasis and reduce swelling; effect. In Fujian and other places, folks use its stems and leaves to make tea and drink water to treat diabetes, etc., and the curative effect is very good. Panax notoginseng is rich in flavonoids and glycosides (Huang Qi et al., Determination of total flavonoid con...

Examples

Embodiment 1

[0024] 1) Extraction and disinfection of explants: select young shoots about 20 days old as explants, cut off the leaves (keep the petioles), clean them with an appropriate amount of detergent, and then rinse them with running water for 1 hour. The clean workbench is sterilized with 70% alcohol for 15 seconds and 2% sodium hypochlorite for 12 minutes, rinsed with sterile water for 5 times, placed in an ultra-clean workbench, and cut into 0.5-1.0cm bud stem segments for later use.

[0025] 2) Induction culture: Inoculate the sterilized explants with buds on the stem segment in the ultra-clean workbench in the MS starting medium without any hormones to induce the production of axillary buds. The culture conditions are 25°C±1°C, and the light intensity is About 2000lx, photoperiod is 16h / 8h, obtains the starting seedling (Fig. 1, A) of 5-8cm height after about 20 days;

[0026] 3) Proliferation culture: use the terminal buds and budding stems of the start-up seedlings as explants...

Embodiment 2

[0030]The specific implementation process is the same as example 1. Wherein the rooting medium formula is MS+IBA 0.3mg / L+IAA 0.3mg / L+sucrose 20g / L+agar 6g / L, can take root (Fig. The seedlings are directly transplanted after hardening; the rooting rate reaches 100%; the transplanting survival rate reaches more than 95%.

technical field

[0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for tissue culture of Panax notoginseng. Background technique

[0002] White-backed Sanqi (Gynura divaricata (L.) DC.) belongs to the genus Panax notoginseng of the Compositae family, also known as Baidongfeng, Baizicao, Yuloquat, Sanbaibang, Thick Noodles, Chicken Vegetables, Big Fat Beef, White Saffron, White Red cabbage, Fugui cabbage, Baizi cabbage, etc. are distributed from Taiwan to South China and Southwest China. Its roots, stems and leaves can be used as medicine; its roots are sweet and cool in nature, can clear away heat and cool blood, dissipate blood stasis and reduce swelling; effect. In Fujian and other places, folks use its stems and leaves to make tea and drink water to treat diabetes, etc., and the curative effect is very good. Panax notoginseng is rich in flavonoids and glycosides (Huang Qi et al., Determination of total flavonoid con...

Examples

Embodiment 1

[0024] 1) Extraction and disinfection of explants: select young shoots about 20 days old as explants, cut off the leaves (keep the petioles), clean them with an appropriate amount of detergent, and then rinse them with running water for 1 hour. The clean workbench is sterilized with 70% alcohol for 15 seconds and 2% sodium hypochlorite for 12 minutes, rinsed with sterile water for 5 times, placed in an ultra-clean workbench, and cut into 0.5-1.0cm bud stem segments for later use.

[0025] 2) Induction culture: Inoculate the sterilized explants with buds on the stem segment in the ultra-clean workbench in the MS starting medium without any hormones to induce the production of axillary buds. The culture conditions are 25°C±1°C, and the light intensity is About 2000lx, photoperiod is 16h / 8h, obtains the starting seedling (Fig. 1, A) of 5-8cm height after about 20 days;

[0026] 3) Proliferation culture: use the terminal buds and budding stems of the start-up seedlings as explants...

Embodiment 2

[0030]The specific implementation process is the same as example 1. Wherein the rooting medium formula is MS+IBA 0.3mg / L+IAA 0.3mg / L+sucrose 20g / L+agar 6g / L, can take root (Fig. The seedlings are directly transplanted after hardening; the rooting rate reaches 100%; the transplanting survival rate reaches more than 95%.

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