Antigen-specific T lymphocyte freezing medium and preparation method and application thereof

A lymphocyte and cryopreservation technology, applied in the field of antigen-specific T lymphocyte cryopreservation and its preparation, can solve the problems of restricting the clinical application of cryopreserved cells, low cell killing function, insufficient cell proliferation, etc. Tumor killing function, the effect of maintaining the survival rate of resuscitation, and improving the cell viability

Active Publication Date: 2017-09-12
郴州宾泽医学检验有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cell recovery rate of this cell cryopreservation program is low after long-term cryopreservation, only about 70%-80%, which directly restricts the clinical application of cryopreserved cells
Moreover, the existing cryopreservati

Method used

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  • Antigen-specific T lymphocyte freezing medium and preparation method and application thereof
  • Antigen-specific T lymphocyte freezing medium and preparation method and application thereof
  • Antigen-specific T lymphocyte freezing medium and preparation method and application thereof

Examples

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Example Embodiment

[0066] The second aspect of the present invention provides a method for preparing an antigen-specific T lymphocyte cryopreservation solution, including:

[0067] The volume fraction of Bomari electrolyte injection, glucose sodium chloride injection, dextran glucose injection and human albumin solution are 30%-40%, 30%-40%, 5%-15% and 15%- Mix at a ratio of 25% to prepare freezing liquid A;

[0068] Put Bomari electrolyte injection, glucose sodium chloride injection, dextran glucose injection, human albumin solution and dimethyl sulfoxide in volume fractions of 20%-30%, 20%-30%, 5%- 15%, 15%-25%, and 10%-20% are mixed to prepare freezing liquid B;

[0069] The cryopreservation solution A is stored separately from the cryopreservation solution B. When in use, the freezing solution A and the freezing solution B are mixed in a volume ratio of 1:0.5-2 to obtain the freezing solution.

[0070] In the present invention, the freezing liquid A and the freezing liquid B are mixed according to ...

Example Embodiment

[0093] Example 1:

[0094] A cryopreservation solution for antigen-specific T lymphocytes, including cryopreservation solution A and cryopreservation solution B, and freezing solution A includes the following components in volume fractions:

[0095] 35% Bomari electrolyte injection, 35% glucose and sodium chloride injection, 10% dextran glucose injection and 20% human albumin solution;

[0096] Cryopreservation Solution B includes the following components in volume fraction:

[0097] 27.5% Bomari electrolyte injection, 27.5% glucose sodium chloride injection, 10% dextran glucose injection, 20% human albumin solution and 15% dimethyl sulfoxide;

[0098] The following is the manufacturer's brand and article number of each raw material used in the embodiment of the present invention:

[0099] Table 1 Table of raw materials

[0100]

[0101] Glucose and sodium chloride injection contains 5% dextrose and 0.45% sodium chloride in mass concentration, and dextran glucose injection contains 10% lo...

Example Embodiment

[0103] Example 2:

[0104] A cryopreservation solution for antigen-specific T lymphocytes, including cryopreservation solution A and cryopreservation solution B, and freezing solution A includes the following components in volume fractions:

[0105] 30% Bomari electrolyte injection, 40% glucose and sodium chloride injection, 5% dextran glucose injection and 25% human albumin solution;

[0106] Cryopreservation Solution B includes the following components in volume fraction:

[0107] 30% Bomari electrolyte injection, 20% glucose and sodium chloride injection, 5% dextran glucose injection, 25% human albumin solution and 20% dimethyl sulfoxide;

[0108] Glucose and sodium chloride injection contains 5% dextrose and 0.45% sodium chloride in mass concentration, and dextran glucose injection contains 10% low molecular weight dextran and 5% dextrose in mass concentration;

[0109] The cryopreservation solution A is stored separately from the cryopreservation solution B. When in use, the freezin...

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Abstract

The invention provides an antigen-specific T lymphocyte freezing medium, comprising freezing medium A and freezing medium B; the freezing medium A includes, by volume, 30-40% of plasmalyte electrolyte injection, 30-40% of glucose and sodium chloride injection, 5-15% of dextran glucose injection and 15-25% of human albumin solution; the freezing medium B includes, by volume, 20-30% of plasmalyte electrolyte injection, 20-30% of glucose and sodium chloride injection, 5-15% of dextran glucose injection, 15-25% of human albumin solution and 10-20% of dimethyl sulfoxide; the freezing medium A and the freezing medium B are stored separately; in use, the freezing medium A and the freezing medium B are mixed in a ratio of 1:(0.5-2), forming the antigen-specific T lymphocyte freezing medium. The antigen-specific T lymphocyte freezing medium is capable of enabling less crystal to form in cells, increasing cell survival rate and maintaining tumor-killing ability of cells. The invention also provides a preparation method and application of the antigen-specific T lymphocyte freezing medium and antigen-specific T lymphocyte injection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an antigen-specific T lymphocyte cryopreservation solution and its preparation method and application. Background technique [0002] Gene-modified immune cell therapy technology is an emerging tumor treatment model, which has become a global research hotspot, especially CAR-T (chimeric antigen receptor T cell) / TCR-T (chimeric T cell) technology. The main representative has become the main development direction of the new century of global biomedicine. [0003] At present, it is believed that the genetically modified immune cells expanded in vitro and then reinfused into the human body are conducive to improving the immune defense ability of the body, which is manifested in that it can improve the body's ability to treat specific diseases such as tumors. In the process of cell immunotherapy, it is not only necessary to cultivate and promote the proliferation of cells, especially afte...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 张长风
Owner 郴州宾泽医学检验有限公司
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