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Antigen-specific T lymphocyte freezing medium and preparation method and application thereof

A lymphocyte and cryopreservation technology, applied in the field of antigen-specific T lymphocyte cryopreservation and its preparation, can solve the problems of restricting the clinical application of cryopreserved cells, low cell killing function, insufficient cell proliferation, etc. Tumor killing function, the effect of maintaining the survival rate of resuscitation, and improving the cell viability

Active Publication Date: 2017-09-12
郴州宾泽医学检验有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cell recovery rate of this cell cryopreservation program is low after long-term cryopreservation, only about 70%-80%, which directly restricts the clinical application of cryopreserved cells
Moreover, the existing cryopreservation methods for immune cells tend to form ice crystals to damage the cells during the cryopreservation process, and there are problems such as low cell viability, insufficient cell proliferation, and low cell killing function after recovery.

Method used

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  • Antigen-specific T lymphocyte freezing medium and preparation method and application thereof
  • Antigen-specific T lymphocyte freezing medium and preparation method and application thereof
  • Antigen-specific T lymphocyte freezing medium and preparation method and application thereof

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preparation example Construction

[0066] The second aspect of the present invention provides a method for preparing an antigen-specific T lymphocyte cryopreservation solution, comprising:

[0067] Bomaili Electrolyte Injection, Glucose Sodium Chloride Injection, Dextran Glucose Injection and Human Serum Albumin Solution are 30%-40%, 30%-40%, 5%-15% and 15%- Mix at a ratio of 25% to prepare freezing solution A;

[0068] Bomaili Electrolyte Injection, Glucose Sodium Chloride Injection, Dextran Glucose Injection, Human Serum Albumin Solution and Dimethyl Sulfoxide are 20%-30%, 20%-30%, 5%- 15%, 15%-25% and 10%-20% were mixed to prepare freezing solution B;

[0069] Freezing solution A and freezing solution B are stored separately. When used, the freezing solution A and freezing solution B are mixed according to the volume ratio of 1:0.5-2 to obtain the freezing solution.

[0070] In the present invention, cryopreservation solution A and cryopreservation solution B are mixed according to a volume ratio of 1:1. ...

Embodiment 1

[0094] A cryopreservation solution for antigen-specific T lymphocytes, including cryopreservation solution A and cryopreservation solution B, where the cryopreservation solution A includes the following volume fraction components:

[0095] 35% Bomaili Electrolyte Injection, 35% Glucose Sodium Chloride Injection, 10% Dextran Glucose Injection and 20% Human Serum Albumin Solution;

[0096] Freezing solution B includes the following components by volume fraction:

[0097] 27.5% Bomaili Electrolyte Injection, 27.5% Glucose Sodium Chloride Injection, 10% Dextran Glucose Injection, 20% Human Serum Albumin Solution and 15% Dimethyl Sulfoxide;

[0098] Below are manufacturer's brand and article number of each raw material that adopts in the embodiment of the present invention:

[0099] Table 1 Raw material list

[0100]

[0101] The glucose sodium chloride injection contains 5% dextrose and 0.45% sodium chloride, and the dextran glucose injection contains 10% low molecular weight...

Embodiment 2

[0104] A cryopreservation solution for antigen-specific T lymphocytes, including cryopreservation solution A and cryopreservation solution B, where the cryopreservation solution A includes the following volume fraction components:

[0105] 30% Bomaili Electrolyte Injection, 40% Glucose Sodium Chloride Injection, 5% Dextran Glucose Injection and 25% Human Serum Albumin Solution;

[0106] Freezing solution B includes the following components by volume fraction:

[0107] 30% Bomaili Electrolyte Injection, 20% Glucose Sodium Chloride Injection, 5% Dextran Glucose Injection, 25% Human Serum Albumin Solution and 20% Dimethyl Sulfoxide;

[0108] The glucose sodium chloride injection contains 5% dextrose and 0.45% sodium chloride, and the dextran glucose injection contains 10% low molecular weight dextran and 5% dextrose;

[0109] Cryopreservation solution A and cryopreservation solution B are stored separately. When in use, cryopreservation solution A and cryopreservation solution B...

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Abstract

The invention provides an antigen-specific T lymphocyte freezing medium, comprising freezing medium A and freezing medium B; the freezing medium A includes, by volume, 30-40% of plasmalyte electrolyte injection, 30-40% of glucose and sodium chloride injection, 5-15% of dextran glucose injection and 15-25% of human albumin solution; the freezing medium B includes, by volume, 20-30% of plasmalyte electrolyte injection, 20-30% of glucose and sodium chloride injection, 5-15% of dextran glucose injection, 15-25% of human albumin solution and 10-20% of dimethyl sulfoxide; the freezing medium A and the freezing medium B are stored separately; in use, the freezing medium A and the freezing medium B are mixed in a ratio of 1:(0.5-2), forming the antigen-specific T lymphocyte freezing medium. The antigen-specific T lymphocyte freezing medium is capable of enabling less crystal to form in cells, increasing cell survival rate and maintaining tumor-killing ability of cells. The invention also provides a preparation method and application of the antigen-specific T lymphocyte freezing medium and antigen-specific T lymphocyte injection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an antigen-specific T lymphocyte cryopreservation solution and its preparation method and application. Background technique [0002] Gene-modified immune cell therapy technology is an emerging tumor treatment model, which has become a global research hotspot, especially CAR-T (chimeric antigen receptor T cell) / TCR-T (chimeric T cell) technology. The main representative has become the main development direction of the new century of global biomedicine. [0003] At present, it is believed that the genetically modified immune cells expanded in vitro and then reinfused into the human body are conducive to improving the immune defense ability of the body, which is manifested in that it can improve the body's ability to treat specific diseases such as tumors. In the process of cell immunotherapy, it is not only necessary to cultivate and promote the proliferation of cells, especially afte...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 张长风
Owner 郴州宾泽医学检验有限公司
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