33results about How to "Reduce cold damage" patented technology

The invention provides an antigen-specific T lymphocyte freezing medium, comprising freezing medium A and freezing medium B; the freezing medium A includes, by volume, 30-40% of plasmalyte electrolyte injection, 30-40% of glucose and sodium chloride injection, 5-15% of dextran glucose injection and 15-25% of human albumin solution; the freezing medium B includes, by volume, 20-30% of plasmalyte electrolyte injection, 20-30% of glucose and sodium chloride injection, 5-15% of dextran glucose injection, 15-25% of human albumin solution and 10-20% of dimethyl sulfoxide; the freezing medium A and the freezing medium B are stored separately; in use, the freezing medium A and the freezing medium B are mixed in a ratio of 1:(0.5-2), forming the antigen-specific T lymphocyte freezing medium. The antigen-specific T lymphocyte freezing medium is capable of enabling less crystal to form in cells, increasing cell survival rate and maintaining tumor-killing ability of cells. The invention also provides a preparation method and application of the antigen-specific T lymphocyte freezing medium and antigen-specific T lymphocyte injection.

The invention discloses a low-temperature-resistant preparation composition for production of cotton, a preparation method and a using method of the low-temperature-resistant preparation composition. The low-temperature-resistant preparation composition for production of the cotton comprises a preparation I and a preparation II; the preparation I comprises a low-temperature-resistant nutrient substance, a plant nutrition promoter, an antifreezing agent, a surfactant and water; and the preparation II comprises a low-temperature-resistant nutrient substance, a plant nutrition promoter, a plant growth regulator, a surfactant and water. The preparation I is mainly used at a seedling stage, and the preparation II is mainly used at blossoming and boll-forming stages. The preparations can well prevent and retard influence and harm of low temperature to production of the cotton, solve the problem of cotton plant growth retarding, cut production and the like caused by low temperature damage in a main cotton production region, guarantee production effectiveness and stable income of cotton planters, are simple in preparation process, easy and convenient to use, environment-friendly and obvious in effect, and can be widely popularized and used in cotton regions.

The invention discloses an aloe extract and application thereof. A preparation method for the aloe extract comprises the following steps: (1) weighing and crushing aloe, soaking the crushed aloe with an aqueous ethanol solution having a concentration of 50 to 100% and accounting for 5 to 15 times of the crushed aloe, then carrying out reflux extraction several times, wherein extraction lasts for 1 to 5 h each time, carrying out filtering to obtain ethanol extracts and filter residues, combining the ethanol extracts, carrying out standing for 12 to 48 h, removing sediments through filtering and recovering the solvent to obtain a crude extract; (2) dissolving the crude extract with water, introducing the dissolved crude extract into a macroporous adsorption resin column, carrying out washing and desugaring, then carrying out elution with the aqueous ethanol solution having a concentration of 50 to 100%, collecting ethanol eluate and recovering the solvent so as to obtain an extract; and (3) dissolving crude extract with water, successively carrying out extraction with dichloromethane, ethyl acetate and n-butanol accounting for 5 to 15 times of the dissolved extract, carrying out concentration and extraction so as to obtain a water phase and then carrying out washing with methanol until a concentrate cannot be dissolved so as to obtain the final extract. The aloe extract provided by the invention can effectively prolong the shelf life of fruit and vegetable, reduce release of ethylene gas, maintain hardness of fruit and vegetable, delay the decrease speed of chlorophyll, decrease moisture evaporation, lower low temperature damage of fruit and vegetable, retain balance between sugar degree and acidity and improve luster of fruit and vegetable.

The invention discloses a self-heating fracturing fluid with controllable peak temperature, and a preparation method and application of the self-heating fracturing fluid. The self-heating fracturing fluid is prepared by mixing a self-heating fracturing fluid base fluid and an activator solution according to a mass ratio of (18-22): (0.8-1.2). According to the self-heating fracturing fluid, a urea/nitrite/ammonium salt/low-concentration acidic activator system is adopted; according to the system, after a low-concentration activator is added, HNO2, NH4<+> and NH3.H2O undergo a thermochemical reaction, so a good heat generation effect is achieved and the cold damage of the fracturing fluid to a stratum is reduced; meanwhile, when the use concentration of an activator solution is 0.005%-0.04%,time for reaching the peak temperature is 1-5 hours, and the peak temperature reaches 48-105 DEG C, so the temperature for reservoir development is controllable, the cold damage of the fracturing fluid to the stratum is reduced, the gel breaking of the fracturing fluid can be enhanced by increasing the temperature, and secondary damage to the stratum is reduced; and therefore, the fracturing fluid is suitable for yield increase and transformation of high-pour-point oil reservoirs and low-temperature reservoirs.

The invention discloses a solid medium used for cornea model low temperature preservation and a preparation method and an application method thereof. The solid medium is prepared by a basic medium and an agarose aqueous solution according to the volume ratio of 1:1 by steps of uniform mixing and coagulation; the mass concentration of the agarose aqueous solution is 0.5-5%; the basic medium takes a mixed solution of a DMEM nutrient solution and a F12 nutrient solution as a basic solution, fetal calf serum, retinoic acid, human epidermal growth factor, insulin, hydrocortisone, adenine, triiodothyronine, glutamine and a low temperature protective agent are added; the low temperature protective agent is a mixture of HEPES, cane sugar, glycine, alanine, adenosine, vitamin E, vitamin C, hyaluronic acid and chondroitin sulfate. The solid medium has the advantage of reducing low temperature anoxic damage on histocyte, can effectively guarantee the accuracy of the test result of a cornea model, and has the advantages of convenient operation and long-distance transport.

The invention discloses a fracturing technology of foam fracturing fluid capable of independently generating heat and gas. The fracturing technology comprises the following steps that S1, the fracturing fluid and a propping agent are selected, and the foam half-life period of the fracturing fluid is measured, wherein the fracturing fluid is prepared from a thickening agent, a cross-linking agent,heat generating agents, a heat generating catalyst, a breaking aid and a gel breaker, the heat generating agents comprise the heat generating agent A and the heat generating agent B, and the heat generating agent A and the heat generating agent B can be subjected to a heat and gas generating reaction under the action of the heat generating catalyst; S2, a fracturing fluid system is prepared; S3, sand fracturing construction is conducted; and S4, well shut-in is conducted according to the foam half-life period, and flowback is conducted after well shut-in is completed. According to the fracturing technology, the permeability of an oil-gas layer is increased through the fracturing fluid capable of independently generating heat and gas the temperature of a reservoir is increased, pressurization and drainage assistance are achieved, the problem of cold damage to the reservoir is effectively solved, viscosity reduction of thickened oil is promoted, and the oil-gas yield is increased.

The invention provides a neural stem cell sphere vitrified cryopreservation and resuscitation method. The method comprises the steps of enabling neural stem cell spheres to be attached to a cell sphere collection piece; then, incubating the cell sphere collection piece in a cryopreservation solution, and then freezing in liquid nitrogen; then, transferring to a cryopreservation tube for storage inliquid nitrogen; during resuscitation, incubating the cell sphere collection piece in a resuscitation medium for resuscitation, culturing in a complete medium of neural stem cells and then subcultring, and culturing and subcultring in a normal manner; the cell sphere collection piece comprises a wall-adherent surface and a support, wherein the wall-adherent surface is rectangular; the support isperpendicularly connected with the short side of the wall-adherent surface and is in an L shape; the height of the support is less than the length of the wall-adherent surface. The neural stem cell sphere vitrified cryopreservation and resuscitation method provided by the invention can bind the neural stem cell spheres to the smaller wall-adherent surface in a high density, thus reaching the precondition of vitrified cryopreservation; furthermore, the cell spheres are attached to the wall in a spreading way, so that the thickness of the cell spheres is effectively reduced, and the penetrationefficiency of a cryoprotectant is increased.

The invention relates to a plugging device, in particular to an oil well plugging device for sealing a wellhead. The oil well plugging device comprises a casing pipe, a pipe column is arranged inside the casing pipe, a switch connecting device and a connecting rod are sequentially arranged above the pipe column, and the connecting rod is movably arranged inside the switch connecting device which is fixedly arranged inside the casing pipe. The oil well plugging device is capable of achieving opening and closing of the pipe column by the aid of a spring column, simple in structure, highly practical and capable of achieving industrial production.

The invention provides compound amino acid fertilizer capable of improving low-temperature cold damages of rice seedlings as well as a preparation method and an application method of the compound amino acid fertilizer and belongs to the technical field of fertilizer. The fertilizer is prepared from the following components in parts by weight: 250 to 350 parts of amino acid powder, 150 to 200 partsof urea, 180 to 300 parts of mixed phosphorus fertilizer, 150 to 250 parts of potassium chloride and 50 to 120 parts of granulation auxiliary agent. The compound amino acid fertilizer provided by theinvention has nutrient resources including N, P, K and the like needed by the growth of plants and also can be used for effectively preventing the low-temperature cold damages at a rice seedling period; meanwhile, the compound amino acid fertilizer has the effects of promoting root activity and tillering, improving the photosynthetic rate of rice and increasing the yield and can be used as a novel fertilizer product for maintaining high production and stable production of the rice in regions which are usually suffered from low temperature at the seedling period, such as rice planting regionsin the middle and lower reaches of the Yangtze River.

The invention provides a cell sphere collecting tube for neural stem cell sphere vitrified cryopreservation. The cell sphere collecting tube has a hollow cylindrical structure with two closed ends, and comprises a tube body, a whorl cover and a gasket; the tube body comprises two layers of tubes which are closely nested with each other and are separately provided with openings at the two ends; inthe two layers of tubes, the outer tube is shorter than the inner tube, and the two ends of the inner tube are provided with external threads; the whorl cover is provided with internal threads matchedwith the external threads of the inner tube; the outer diameter of the whorl cover is the same as that of the outer tube; the gasket sleeves the outer side of the inner tube; the outer diameter of the gasket is the same as that of the outer tube. The invention also provides a neural stem cell sphere vitrified cryopreservation and resuscitation method adopting the cell sphere collecting tube. After the neural stem cell sphere vitrified cryopreservation and resuscitation method and the device are adopted, the vitrified cryopreservation of neural stem cells can be efficiently completed.

The invention discloses application of fibroin and a fruit and vegetable storage method. The fibroin is applied to the preparation of a fruit and vegetable anti-cold-injury agent. The fruit and vegetable storage method comprises the following step that the fruit and vegetable anti-cold-injury agent containing the fibroin is applied on the surfaces of fruits and vegetables. According to the scheme,the fruit and vegetable anti-cold-injury agent has a good application effect on the storage of bananas and other fruits and vegetables. According to the technical scheme, the utilized fibroin can form a layer of edible protein film on the surfaces of the fruits and vegetables, the stable structure and excellent mechanical barrier characteristics of the film can be kept due to the fact that the fibroin contains a large number of hydrogen bonds and disulfide bonds and has hydrophobic interaction force, and the fibroin can significantly inhibit the decrease of cell membrane lipids in fruit peeltissue, thereby reducing the symptoms of cold injuries. The fibroin is natural, nontoxic and harmless, is easily soluble in water, and does not need to be treated with other chemical additives, therefore, after the fruit and vegetable anti-cold-injury agent is adopted, no harmful chemical substances can remain on the surfaces of the fruits and vegetables, so that the fruits and vegetables are safer and healthier, and the edible film formed by the fibroin is completely soluble in the water and does not pollute the water after use.

The invention discloses a manifold device. The manifold device is arranged on the ground, and used for transporting a high-pressure medium to a stratum. The manifold device comprises a main pipe and auxiliary pipes, wherein each auxiliary pipe comprises a hard pipeline, a check valve and a hard pipe elbow which are sequentially connected. According to the invention, each of the main pipe, the hard pipeline and the hard pipe elbow of the manifold device is designed into a multilayer structure formed by arranging an inner core pipe layer, a low-temperature-resistant protection layer and a pipe body layer in a bonding manner from the inside to the outside; each inner core pipe layer is made from a low-temperature-resistant alloy steel material; and each low-temperature-resistant protection layer is made from a foam glass material. Each pipe body with the multilayer structure has a low-temperature-resistant function, and a risk of breakage due to cold damages of a low-temperature medium to the pipe body can be greatly reduced, and equipment losses, personal injuries and environment pollution due to the breakage of the pipe body are avoided; and moreover, each pipe body with the multilayer structure and the low-temperature-resistant protection function is capable of isolating a heat conduction action thereof with the outside, and reducing the cold losses of the low-temperature high-pressure medium, so as to achieve a temperature of construction requirements; and construction efficiency is increased.

The present invention discloses a peach polygalacturonase-inhibiting protein PpPGIP1 gene, and a cloning method and use thereof. The peach polygalacturonase-inhibiting protein PpPGIP1 gene has a nucleotide sequence shown in SEQ ID NO: 1, and a protein encoded by the peach polygalacturonase-inhibiting protein PpPGIP1 gene has an amino acid sequence shown in SEQ ID NO: 2. The cloning method includes the following steps: (1) extracting total RNA from a peach, and subjecting the total RNA to reverse transcription to obtain cDNA, which serves as a template; (2) designing primers based on the PpPGIP1 gene sequence; and (3) Polymerase Chain Reaction (PCR) amplification: conducting PCR amplification to obtain a PpPGIP1 gene amplification product. As there is a protein-protein interaction relationship between PpPGIP1 and PpVIN2, the effective inhibition of the PpPGIP1 expression in peach can significantly reduce the activity of the acid invertase PpVIN2 and thus reduce the decomposition of sucrose.

This composition can be easily added when cryopreserving cells and improves cell viability. 1 volume % of the composition containing 0.01 wt % to 20 wt % of a sophorose lipid is added just before or up to 6 hours before cryopreservation and cells are stored. This composition can improve cryopreservation cell viability as it reduces freezing damage to cells. With this composition, cells can be stored without using DMSO and blood serum. This is a simple and inexpensive storage method as it does not require fine control of the freezing rate during cell cryopreservation.