34results about How to "Reduce cold damage" patented technology

The invention provides an antigen-specific T lymphocyte freezing medium, comprising freezing medium A and freezing medium B; the freezing medium A includes, by volume, 30-40% of plasmalyte electrolyte injection, 30-40% of glucose and sodium chloride injection, 5-15% of dextran glucose injection and 15-25% of human albumin solution; the freezing medium B includes, by volume, 20-30% of plasmalyte electrolyte injection, 20-30% of glucose and sodium chloride injection, 5-15% of dextran glucose injection, 15-25% of human albumin solution and 10-20% of dimethyl sulfoxide; the freezing medium A and the freezing medium B are stored separately; in use, the freezing medium A and the freezing medium B are mixed in a ratio of 1:(0.5-2), forming the antigen-specific T lymphocyte freezing medium. The antigen-specific T lymphocyte freezing medium is capable of enabling less crystal to form in cells, increasing cell survival rate and maintaining tumor-killing ability of cells. The invention also provides a preparation method and application of the antigen-specific T lymphocyte freezing medium and antigen-specific T lymphocyte injection.

The invention provides a steam-huff-and-puff oil production method assisted by injection of air and carbon dioxide. The method includes the following steps that (1) an injection well and an adjacent well are arranged, and the oxygen content of casing pipes of the injection well and the adjacent well is measured online; (2) when the online measured oxygen content of the injection well casing pipe is higher than an explosion point, nitrogen and / or carbon dioxide are / is injected into the injection well, and the content of oxygen content of the injection well casing pipe, which is lower than the explosion point, is an injection finishing point; (3) the gas mixture of air and carbon dioxide is injected till the total reaches a design discharge, and after the injection is finished, a carbon dioxide slug killing well is used, and then steam is injected; or a gas mixture slug of air and carbon dioxide is injected, a steam slug is injected, then the gas mixture slug and the steam slug are injected continuously, circularly and alternately till the gas mixture slug injection total reaches the design discharge, and after the circular injection is finished, the carbon dioxide slug killing well is used; (4) the well is soaked till the pressure inside the well reaches a normal pressure, and blowout production is carried out.

The invention discloses a low-temperature-resistant preparation composition for production of cotton, a preparation method and a using method of the low-temperature-resistant preparation composition. The low-temperature-resistant preparation composition for production of the cotton comprises a preparation I and a preparation II; the preparation I comprises a low-temperature-resistant nutrient substance, a plant nutrition promoter, an antifreezing agent, a surfactant and water; and the preparation II comprises a low-temperature-resistant nutrient substance, a plant nutrition promoter, a plant growth regulator, a surfactant and water. The preparation I is mainly used at a seedling stage, and the preparation II is mainly used at blossoming and boll-forming stages. The preparations can well prevent and retard influence and harm of low temperature to production of the cotton, solve the problem of cotton plant growth retarding, cut production and the like caused by low temperature damage in a main cotton production region, guarantee production effectiveness and stable income of cotton planters, are simple in preparation process, easy and convenient to use, environment-friendly and obvious in effect, and can be widely popularized and used in cotton regions.

The invention discloses an aloe extract and application thereof. A preparation method for the aloe extract comprises the following steps: (1) weighing and crushing aloe, soaking the crushed aloe with an aqueous ethanol solution having a concentration of 50 to 100% and accounting for 5 to 15 times of the crushed aloe, then carrying out reflux extraction several times, wherein extraction lasts for 1 to 5 h each time, carrying out filtering to obtain ethanol extracts and filter residues, combining the ethanol extracts, carrying out standing for 12 to 48 h, removing sediments through filtering and recovering the solvent to obtain a crude extract; (2) dissolving the crude extract with water, introducing the dissolved crude extract into a macroporous adsorption resin column, carrying out washing and desugaring, then carrying out elution with the aqueous ethanol solution having a concentration of 50 to 100%, collecting ethanol eluate and recovering the solvent so as to obtain an extract; and (3) dissolving crude extract with water, successively carrying out extraction with dichloromethane, ethyl acetate and n-butanol accounting for 5 to 15 times of the dissolved extract, carrying out concentration and extraction so as to obtain a water phase and then carrying out washing with methanol until a concentrate cannot be dissolved so as to obtain the final extract. The aloe extract provided by the invention can effectively prolong the shelf life of fruit and vegetable, reduce release of ethylene gas, maintain hardness of fruit and vegetable, delay the decrease speed of chlorophyll, decrease moisture evaporation, lower low temperature damage of fruit and vegetable, retain balance between sugar degree and acidity and improve luster of fruit and vegetable.

The invention provides a gas injection and shallow super oil production integrated device,which includes tubing string and sucker rod string. Bottom of tubing string is connected with an injection and production integrated high angle pump of which bottom is connected with a chamfer tubing through a reducer union. Bottom of the chamfer tubing is connected with shoe guide plug. Flow regulator is arranged on the chamfer tubing. Bottom of anti-corrosion and anti-wear rod string is connected with anti-off-screw device of which bottom is connected with anti-corrosion weighing rod of which bottom is connected with anti-off-screw device of which bottom is connected with anti-corrosion and anti-wear sucker rod string and plunger piston. The anti-off-screw device is arranged at two sides of the anti-corrosion and anti-wear sucker rod string. The device achieves throughout and spinning of immobile string, which not only reduces operation times but also avoids cooling damage caused by operation liquid for stratum during the process of spinning operation. Moreover, the device ensures throughout and spinning effect and meanwhile significantly reduces costs of gas injection and oil production.

The invention discloses a self-heating fracturing fluid with controllable peak temperature, and a preparation method and application of the self-heating fracturing fluid. The self-heating fracturing fluid is prepared by mixing a self-heating fracturing fluid base fluid and an activator solution according to a mass ratio of (18-22): (0.8-1.2). According to the self-heating fracturing fluid, a urea / nitrite / ammonium salt / low-concentration acidic activator system is adopted; according to the system, after a low-concentration activator is added, HNO2, NH4<+> and NH3.H2O undergo a thermochemical reaction, so a good heat generation effect is achieved and the cold damage of the fracturing fluid to a stratum is reduced; meanwhile, when the use concentration of an activator solution is 0.005%-0.04%,time for reaching the peak temperature is 1-5 hours, and the peak temperature reaches 48-105 DEG C, so the temperature for reservoir development is controllable, the cold damage of the fracturing fluid to the stratum is reduced, the gel breaking of the fracturing fluid can be enhanced by increasing the temperature, and secondary damage to the stratum is reduced; and therefore, the fracturing fluid is suitable for yield increase and transformation of high-pour-point oil reservoirs and low-temperature reservoirs.

The invention discloses a solid medium used for cornea model low temperature preservation and a preparation method and an application method thereof. The solid medium is prepared by a basic medium and an agarose aqueous solution according to the volume ratio of 1:1 by steps of uniform mixing and coagulation; the mass concentration of the agarose aqueous solution is 0.5-5%; the basic medium takes a mixed solution of a DMEM nutrient solution and a F12 nutrient solution as a basic solution, fetal calf serum, retinoic acid, human epidermal growth factor, insulin, hydrocortisone, adenine, triiodothyronine, glutamine and a low temperature protective agent are added; the low temperature protective agent is a mixture of HEPES, cane sugar, glycine, alanine, adenosine, vitamin E, vitamin C, hyaluronic acid and chondroitin sulfate. The solid medium has the advantage of reducing low temperature anoxic damage on histocyte, can effectively guarantee the accuracy of the test result of a cornea model, and has the advantages of convenient operation and long-distance transport.

The invention discloses a fracturing technology of foam fracturing fluid capable of independently generating heat and gas. The fracturing technology comprises the following steps that S1, the fracturing fluid and a propping agent are selected, and the foam half-life period of the fracturing fluid is measured, wherein the fracturing fluid is prepared from a thickening agent, a cross-linking agent,heat generating agents, a heat generating catalyst, a breaking aid and a gel breaker, the heat generating agents comprise the heat generating agent A and the heat generating agent B, and the heat generating agent A and the heat generating agent B can be subjected to a heat and gas generating reaction under the action of the heat generating catalyst; S2, a fracturing fluid system is prepared; S3, sand fracturing construction is conducted; and S4, well shut-in is conducted according to the foam half-life period, and flowback is conducted after well shut-in is completed. According to the fracturing technology, the permeability of an oil-gas layer is increased through the fracturing fluid capable of independently generating heat and gas the temperature of a reservoir is increased, pressurization and drainage assistance are achieved, the problem of cold damage to the reservoir is effectively solved, viscosity reduction of thickened oil is promoted, and the oil-gas yield is increased.

The invention provides a neural stem cell sphere vitrified cryopreservation and resuscitation method. The method comprises the steps of enabling neural stem cell spheres to be attached to a cell sphere collection piece; then, incubating the cell sphere collection piece in a cryopreservation solution, and then freezing in liquid nitrogen; then, transferring to a cryopreservation tube for storage inliquid nitrogen; during resuscitation, incubating the cell sphere collection piece in a resuscitation medium for resuscitation, culturing in a complete medium of neural stem cells and then subcultring, and culturing and subcultring in a normal manner; the cell sphere collection piece comprises a wall-adherent surface and a support, wherein the wall-adherent surface is rectangular; the support isperpendicularly connected with the short side of the wall-adherent surface and is in an L shape; the height of the support is less than the length of the wall-adherent surface. The neural stem cell sphere vitrified cryopreservation and resuscitation method provided by the invention can bind the neural stem cell spheres to the smaller wall-adherent surface in a high density, thus reaching the precondition of vitrified cryopreservation; furthermore, the cell spheres are attached to the wall in a spreading way, so that the thickness of the cell spheres is effectively reduced, and the penetrationefficiency of a cryoprotectant is increased.

The invention relates to a plugging device, in particular to an oil well plugging device for sealing a wellhead. The oil well plugging device comprises a casing pipe, a pipe column is arranged inside the casing pipe, a switch connecting device and a connecting rod are sequentially arranged above the pipe column, and the connecting rod is movably arranged inside the switch connecting device which is fixedly arranged inside the casing pipe. The oil well plugging device is capable of achieving opening and closing of the pipe column by the aid of a spring column, simple in structure, highly practical and capable of achieving industrial production.

The invention discloses a method for preserving agaricus bisporus for long term, which is characterized by employing an antistaling agent for processing the fresh agaricus bisporus, the antistaling agent comprises the following raw materials by mass fraction: 50-70% of citric acid, 15-45% of sodium bisulfite, 5-10% of balsam pear extract product, 5-10% of lotus leaf extract product, 0.025-0.03% of natural color protection agent, 1-5% of water-soluble vitamin and 0.01-0.1% of trace element; wherein sum of mass fraction of the raw materials is 100%. The method can effectively prolong the fresh-keeping period of the agaricus bisporus, reduce the release of ethane, keep the hardness of the agaricus bisporus, delay the decreasing speed of chlorophyll, reduce moisture evaporation, reduce the low temperature damage on the agaricus bisporus, keep the balance of saccharic acid degree, reduce the browning due to scratch and block the harmful mushroom poison to prevent propagation harm, prevent mildew, and increase the luster of the agaricus bisporus.

The invention provides compound amino acid fertilizer capable of improving low-temperature cold damages of rice seedlings as well as a preparation method and an application method of the compound amino acid fertilizer and belongs to the technical field of fertilizer. The fertilizer is prepared from the following components in parts by weight: 250 to 350 parts of amino acid powder, 150 to 200 partsof urea, 180 to 300 parts of mixed phosphorus fertilizer, 150 to 250 parts of potassium chloride and 50 to 120 parts of granulation auxiliary agent. The compound amino acid fertilizer provided by theinvention has nutrient resources including N, P, K and the like needed by the growth of plants and also can be used for effectively preventing the low-temperature cold damages at a rice seedling period; meanwhile, the compound amino acid fertilizer has the effects of promoting root activity and tillering, improving the photosynthetic rate of rice and increasing the yield and can be used as a novel fertilizer product for maintaining high production and stable production of the rice in regions which are usually suffered from low temperature at the seedling period, such as rice planting regionsin the middle and lower reaches of the Yangtze River.

The invention provides a cell sphere collecting tube for neural stem cell sphere vitrified cryopreservation. The cell sphere collecting tube has a hollow cylindrical structure with two closed ends, and comprises a tube body, a whorl cover and a gasket; the tube body comprises two layers of tubes which are closely nested with each other and are separately provided with openings at the two ends; inthe two layers of tubes, the outer tube is shorter than the inner tube, and the two ends of the inner tube are provided with external threads; the whorl cover is provided with internal threads matchedwith the external threads of the inner tube; the outer diameter of the whorl cover is the same as that of the outer tube; the gasket sleeves the outer side of the inner tube; the outer diameter of the gasket is the same as that of the outer tube. The invention also provides a neural stem cell sphere vitrified cryopreservation and resuscitation method adopting the cell sphere collecting tube. After the neural stem cell sphere vitrified cryopreservation and resuscitation method and the device are adopted, the vitrified cryopreservation of neural stem cells can be efficiently completed.

The invention discloses application of fibroin and a fruit and vegetable storage method. The fibroin is applied to the preparation of a fruit and vegetable anti-cold-injury agent. The fruit and vegetable storage method comprises the following step that the fruit and vegetable anti-cold-injury agent containing the fibroin is applied on the surfaces of fruits and vegetables. According to the scheme,the fruit and vegetable anti-cold-injury agent has a good application effect on the storage of bananas and other fruits and vegetables. According to the technical scheme, the utilized fibroin can form a layer of edible protein film on the surfaces of the fruits and vegetables, the stable structure and excellent mechanical barrier characteristics of the film can be kept due to the fact that the fibroin contains a large number of hydrogen bonds and disulfide bonds and has hydrophobic interaction force, and the fibroin can significantly inhibit the decrease of cell membrane lipids in fruit peeltissue, thereby reducing the symptoms of cold injuries. The fibroin is natural, nontoxic and harmless, is easily soluble in water, and does not need to be treated with other chemical additives, therefore, after the fruit and vegetable anti-cold-injury agent is adopted, no harmful chemical substances can remain on the surfaces of the fruits and vegetables, so that the fruits and vegetables are safer and healthier, and the edible film formed by the fibroin is completely soluble in the water and does not pollute the water after use.

The invention discloses a jack fruit pulp freezing and slicing method. The jack fruit pulp freezing and slicing method comprises the following steps of carrying out fixing treatment on jack fruit pulpby using a sucrose-FAA fixing solution, and then performing immersion cleaning by using a phosphoric acid buffer solution of sucrose for carrying out infiltration protection on the fixed sample tissues; and putting into glycerol, carrying out vacuum extraction, culturing by a culture box, jointly treating the jack fruit pulp sample by using a mixed embedding agent, putting the embedded sample into liquid nitrogen for quick-freezing, putting the quick-frozen sample in a freezing chamber for temperature rise, and carrying out slicing and dyeing. According to the method disclosed by the invention, all steps are closely related and mutually cooperated, the operation steps of the existing freezing slicing method are optimized, the experimental steps are reduced, the experiment time is shortened, the generation of ice crystal of the jackfruit pulp cells is reduced, the breaking of cells is reduced, the integrity of the cell structure and the tissue structure is guaranteed, and a clear pulptissue slicing diagram can be obtained.

The invention discloses a manifold device. The manifold device is arranged on the ground, and used for transporting a high-pressure medium to a stratum. The manifold device comprises a main pipe and auxiliary pipes, wherein each auxiliary pipe comprises a hard pipeline, a check valve and a hard pipe elbow which are sequentially connected. According to the invention, each of the main pipe, the hard pipeline and the hard pipe elbow of the manifold device is designed into a multilayer structure formed by arranging an inner core pipe layer, a low-temperature-resistant protection layer and a pipe body layer in a bonding manner from the inside to the outside; each inner core pipe layer is made from a low-temperature-resistant alloy steel material; and each low-temperature-resistant protection layer is made from a foam glass material. Each pipe body with the multilayer structure has a low-temperature-resistant function, and a risk of breakage due to cold damages of a low-temperature medium to the pipe body can be greatly reduced, and equipment losses, personal injuries and environment pollution due to the breakage of the pipe body are avoided; and moreover, each pipe body with the multilayer structure and the low-temperature-resistant protection function is capable of isolating a heat conduction action thereof with the outside, and reducing the cold losses of the low-temperature high-pressure medium, so as to achieve a temperature of construction requirements; and construction efficiency is increased.

The invention provides an antigen-specific T lymphocyte freezing medium, comprising freezing medium A and freezing medium B; the freezing medium A includes, by volume, 30-40% of plasmalyte electrolyte injection, 30-40% of glucose and sodium chloride injection, 5-15% of dextran glucose injection and 15-25% of human albumin solution; the freezing medium B includes, by volume, 20-30% of plasmalyte electrolyte injection, 20-30% of glucose and sodium chloride injection, 5-15% of dextran glucose injection, 15-25% of human albumin solution and 10-20% of dimethyl sulfoxide; the freezing medium A and the freezing medium B are stored separately; in use, the freezing medium A and the freezing medium B are mixed in a ratio of 1:(0.5-2), forming the antigen-specific T lymphocyte freezing medium. The antigen-specific T lymphocyte freezing medium is capable of enabling less crystal to form in cells, increasing cell survival rate and maintaining tumor-killing ability of cells. The invention also provides a preparation method and application of the antigen-specific T lymphocyte freezing medium and antigen-specific T lymphocyte injection.

The invention discloses a solid medium used for cornea model low temperature preservation and a preparation method and an application method thereof. The solid medium is prepared by a basic medium and an agarose aqueous solution according to the volume ratio of 1:1 by steps of uniform mixing and coagulation; the mass concentration of the agarose aqueous solution is 0.5-5%; the basic medium takes a mixed solution of a DMEM nutrient solution and a F12 nutrient solution as a basic solution, fetal calf serum, retinoic acid, human epidermal growth factor, insulin, hydrocortisone, adenine, triiodothyronine, glutamine and a low temperature protective agent are added; the low temperature protective agent is a mixture of HEPES, cane sugar, glycine, alanine, adenosine, vitamin E, vitamin C, hyaluronic acid and chondroitin sulfate. The solid medium has the advantage of reducing low temperature anoxic damage on histocyte, can effectively guarantee the accuracy of the test result of a cornea model, and has the advantages of convenient operation and long-distance transport.

The invention discloses a forced segmented injection-production pipe string and a method for a thermal recovery well. The injection-production pipe string includes a steam injection and oil production integrated pump sequentially connected to the oil pipe from top to bottom, a self-regulating uniform steam injection device, a self-regulating The oil well liquid inlet device and the steam injection pipe string plug, the self-adjusting uniform steam injection device and the self-adjusting oil well liquid inlet device are taken as a group, and the number is set in at least two groups, and the self-adjusting uniform steam injection device, self-adjusting The oil well liquid inlet device corresponds to the same section of injection-production layer at the same time, and the oil pipe is also connected with a steam injection expansion packer, which is sealed on the inner wall of the thermochemical sealing rubber plug in the casing. The number of vapor expansion packers and thermochemical sealing plugs is consistent and at least three are set, and each thermochemical sealing plug can force the injection-production layer to be segmented. It can overcome problems such as uneven production, steam channeling and high water cut caused by heterogeneity in the process of steam injection and production in horizontal wells, thereby improving the overall recovery rate and economic benefits of the oil field.

The invention discloses a method for establishing an efficient plant regeneration system by using a hevea brasiliensis embryonic cell suspension system. The method comprises the steps of friable embryonic callus induction with embryonic suspension cells, friable embryonic callus subculture, differentiation of body cell embryoid, plant regeneration and the like. The method is simple in process flow, low in production cost, high in embryoid inductivity and high in plant regeneration rate, and has great application value.

The invention provides a low-permeability thick-oil oil reservoir fracturing method. The low-permeability thick-oil oil reservoir fracturing method comprises the following steps that reservoir rock mechanical parameters and logging data are obtained, the fracturing crack parameters are obtained through simulation, and the needed viscosity-decreasing liquid preparation quantity is obtained throughcalculation; according to data of adjacent wells and the logging data, the stratum fracture pressure of a transformed layer section of a fracturing well is calculated, and the highest injection displacement Qmax of viscosity-decreasing liquid when a reservoir is not fractured open is calculated; the viscosity-decreasing liquid is prepared from hot water, the viscosity-decreasing liquid is injectedinto the reservoir at the injection displacement being 0.9 time the Qmax at the matrix injection stage, after the matrix injection stage is finished, the injection displacement is increased to fracture the reservoir open, and the remaining viscosity-decreasing liquid continues being injected into the reservoir to enable the impact range of the viscosity-decreasing liquid to be larger than that ofhydraulic cracks; and after injection of the viscosity-decreasing liquid is finished, well kill is conducted for 2-5 days, then fracturing liquid is prepared from warn water, fracturing transformingis conducted on a thick-oil well according to the end sand removal transforming mode, and after fracturing transforming is finished, the well is closed for 2 h to conduct rapid back flow.

The invention discloses a cuttage breeding method for improving cold resistance of pitaya, which comprises the following steps: S1, selecting robust branches, cutting off the branches, and carrying out cuttage to obtain pitaya rooting seedlings; s2, transplanting the pitaya rooted seedlings into a plastic greenhouse, immediately irrigating the pitaya rooted seedlings with root fixing water, and spraying a nutrient solution after 2-5 days; s3, controlling the temperature in the greenhouse to be 5-9 DEG C, and spraying a nutrient solution; after the seedlings are cultured for 3-4 months, removing dead seedlings, adjusting the temperature to be-5 DEG C to 5 DEG C, and spraying a nutrient solution; continuously culturing for 3-4 months, and removing dead nursery stocks at the same time; s4, transplanting the seedlings subjected to low-temperature treatment into a natural environment for continuous growth, harvesting after seeds are mature, continuously sowing the cold-resistant pitaya, selfing the cold-resistant pitaya, and selecting branches or seeds with excellent and stable characters from offspring of the cold-resistant pitaya to cultivate the cold-resistant pitaya. The problem of poor cold resistance of pitaya can be solved, and the low-temperature treatment survival rate and yield of pitaya are improved.

The invention provides a preservation solution for an erythrocyte. The preservation solution is prepared from the following raw materials with concentrations: 10.0g / L to 40.0g / L disaccharide, 1.0g / L to 3.0g / L sodium chloride, 3.0g / L to 10.Og / L EDTA-Na2 (Disodium Ethylenediaminetetraacetate), 5.0g / L to 10.0g / L glycine, 0.1g / L to 0.3g / L of KH2PO4, 5g / L to 10g / L Na2HPO4.12H2O, 30mg / L to 90mg / L of sulfanilamide, 10mg / L to 30mg / L trimethoprim and 0.15g / L to 1.3g / L antioxidant, wherein the pH (potential of Hydrogen) is 7.5 to 8.5; the osmotic pressure is 240mosm to 380mosm. The preservation solutionfor the erythrocyte, which is provided by the invention, can be used for obviously decreasing the hemolysis ratio of the erythrocyte during preservation, is used for prolonging the preservation timeof the erythrocyte and can be used for avoiding the aggregation phenomenon of the erythrocyte during in-vitro preservation and the preservation can reach 120 days at 2 to 8 DEG C.

The present invention discloses a peach polygalacturonase-inhibiting protein PpPGIP1 gene, and a cloning method and use thereof. The peach polygalacturonase-inhibiting protein PpPGIP1 gene has a nucleotide sequence shown in SEQ ID NO: 1, and a protein encoded by the peach polygalacturonase-inhibiting protein PpPGIP1 gene has an amino acid sequence shown in SEQ ID NO: 2. The cloning method includes the following steps: (1) extracting total RNA from a peach, and subjecting the total RNA to reverse transcription to obtain cDNA, which serves as a template; (2) designing primers based on the PpPGIP1 gene sequence; and (3) Polymerase Chain Reaction (PCR) amplification: conducting PCR amplification to obtain a PpPGIP1 gene amplification product. As there is a protein-protein interaction relationship between PpPGIP1 and PpVIN2, the effective inhibition of the PpPGIP1 expression in peach can significantly reduce the activity of the acid invertase PpVIN2 and thus reduce the decomposition of sucrose.

The invention provides a cell spheroid collection tube for vitrification storage of neural stem cell spheroids, which is a hollow cylindrical structure with both ends closed, including a tube body, a screw cap and a gasket; the tube body is a tightly fitted double layer tube with openings at both ends; the outer tube of the double-layer tube is shorter than the inner tube, and the two ends of the inner tube have outer helices; the screw cap has an inner helix matching the outer helix of the inner tube, and the helix The outer diameter of the cover is the same as that of the outer tube; the gasket is sleeved on the outside of the inner tube, and the outer diameter of the gasket is the same as that of the outer tube. The present invention also provides a method for vitrification and recovery of neural stem cell spheres using the above-mentioned cell sphere collection tube. By adopting the vitrification preservation and recovery method and device of the neural stem cell spheres of the present invention, the vitrification preservation of the neural stem cells can be efficiently completed.

The invention discloses a rainwater three-state high-efficiency collection device based on an agricultural greenhouse, which belongs to the field of rainwater collection technology, can realize a breakthrough in the traditional collection of liquid rainwater, and innovatively collect gaseous water and solid snow as well, using the collection pipe The unique self-freezing filter screen can not only filter impurities in rain and snow, but also retain water droplets through the dynamic water-conducting fibers on the self-freezing filter screen in heavy snow weather. A thin layer of water film is formed on the ice filter, and it freezes autonomously in a low-temperature environment to form an isolated ice layer that isolates the environment inside and outside the greenhouse. It is used for heat preservation in the environment in the greenhouse to reduce low-temperature damage, and at the same time can support a certain thickness of snow. Auxiliary to keep the greenhouse warm, and when there is a lot of snow, the weight becomes larger and oppresses the ice layer. The snow is separated into dots and falls under the action of the self-freezing filter, and quickly turns into water in the greenhouse and is collected to complete the rainwater protection. The three-state efficient collection of .

The invention provides a cell ball collecting tube for vitrification cryopreservation of neural stem cell balls. The cell ball collecting tube is a hollow cylindrical structure with two closed ends, and comprises a tube body, rotary covers and gaskets; the tube body is a double-layer tube formed by tight sleeve, and two ends of the tube body are open; an outer tube of the double-layer tube is shorter than an inner tube; two ends of the inner tube are provided with an external spiral; each rotary cover is provided with an internal spiral which is in screw match with the corresponding external spiral of the inner pipe; the outer diameter of each rotary cover is the same as the outer diameter of the outer pipe; the gaskets sleeve the outer side of the inner pipe; and the outer diameter of thegasket is the same as the outer diameter of the outer pipe. The invention further provides a method for vitrification cryopreservation and thawing of neural stem cell balls by adopting the cell ballcollecting tube. Through the adoption of the method and a device for vitrification cryopreservation and thawing of neural stem cell balls, the vitrification cryopreservation of the neural stem cells can be efficiently completed.

The invention relates to the technical field of blood storage equipment, in particular to an automatic blood access box structure. The automatic blood access box structure comprises a box body, a cover plate, a drive mechanism, a partition plate, a blocking space, a floating plate, a blocking block, a limiting slot, a floating column and an elastic part. The automatic blood access box structure has the beneficial effects that: while the cover plate is opened, the elastic block abuts against the blocking block, so that the floating plate moves upwards, and therefore, hands of medical staff do not need to extend into the box body while the medical staff places or takes a blood storage bag, and low-temperature harm, on the medical staff, is at least reduced to certain extent; after the coverplate is covered, the surface of the cover plate abuts against the upper end of the floating column for driving the floating plate to move downwards, so that the floating plate is automatically blocked into the blocking space, and therefore, operations are convenient, and the structure is simple; and a drive element acts to drive a connector to swing, so that the cover plate is driven to rotate along a hinged part with the box body, and therefore, opening and closing of the cover plate are realized, and the medical staff does not need to manually open the box.