56 results about "Body cell" patented technology

The invention relates to the technical field of nuclear reactor fuel element analysis, in particular to an irradiated zirconium alloy plasticity limit prediction method based on a crystal plasticity finite element, which comprises the following steps: establishing a three-dimensional polycrystalline representative body cell model conforming to the actual grain size of zirconium alloy; based on a crystal plasticity theoretical model, zirconium alloy material parameters are obtained through calculation; boundary conditions and load conditions are determined, finite element calculation is executed in combination with material parameters, and an element stiffness matrix of each increment step is output; assembling the unit stiffness matrix, and obtaining a macroscopic stress-strain matrix of the three-dimensional polycrystal cell model through a homogenization method; and substituting the macroscopic stress-strain matrix into the Rice theoretical criterion, traversing the macroscopic stress-strain matrix of each increment step, and determining the plastic strain meeting the judgment critical condition. According to the method, accurate finite element plastic limit analysis can be carried out on the irradiation material, and the plastic limit strain of the zirconium alloy under the irradiation condition can be accurately judged.

The application relates to a kind of gland cell image segmentation methods based on selective multi-branch hollow convolution, input gland cell image dataset, with improved UNet as main network, construct gland cell segmentation network based on selective multi-branch hollow convolution, the network is captured more advanced features with context encoding network, with channel attention module adaptive recalibration channel feature response and highlight the most relevant feature channel, with multi-scale selection module amplifies effective information and suppresses redundant information, selects the appropriate scale of hollow convolution branch;With the input gland cell image dataset, the segmentation network is trained to obtain a stable segmentation network;Input to be segmented gland cell image, with stable segmentation network to carry out gland cell segmentation.The application has better generalization ability, and is especially suitable for the size, shape change difference of gland in colorectal polyp.

The utility model discloses a cell slide culture device, relates to cell culture technical field, including box body, cell slide, slide groove, upper cover and screw, said box body is provided with a plurality of slide groove, said slide groove is placed with cell slide, said box body both sides is provided with a plurality of threaded hole, said upper cover is equipped with the screw, said upper cover is equipped with the screw, said upper cover is equipped with the screw, said upper cover is equipped with the screw. A clamping groove is formed in the edge of the top end of the box body in a surrounding mode, the upper cover is integrally embedded into the clamping groove, and the screws penetrate through the upper cover to be matched with the threaded holes. The cell climbing piece is simple to operate, convenient to use and easy to take and place from the box body, the situation that cells fall off from the cell climbing piece is reduced, and an operator can conveniently carry the cell climbing piece outside a laboratory.

Provided herein are, inter alia, methods for preparing a liquid cell culture media that has lesser lot-to-lot analytical variation, increased performance, and has lesser metal ion concentrations compared to a liquid media prepared by traditional methods. Such liquid media may be used for culturing cells, including but not limited to, recombinant cells.

The embodiment of the specification relates to the technical field of super-resolution fluorescence microscopy, and particularly relates to an optical superlens optical sheet endoscopy imaging system and method. Phase control is performed on short-wavelength laser by an optical sheet superlens, so that incident light on a sample is finely adjusted into the form of an optical sheet, the short-wavelength laser after phase control is irradiated on the sample, the optical sheet and the sample interact to excite fluorescence, at the same time, phase control is performed on long-wavelength laser by an OAM light superlens, the obtained OAM light is irradiated on the sample, the OAM light quenches fluorescent substances at a specified position in a fluorescent spot excited by the short-wavelength laser, so that the diffraction area of the fluorescent spot is reduced, finally, fluorescence remaining after OAM light irradiation of the sample is converged on a photodiode for fluorescence power detection, and the resolution of imaging is improved on the basis of scanning of samples such as living cells.

The present disclosure describes systems, devices, and methods for high-throughput electroporation of a donor cell. The systems can include a perforated membrane having one or more layers, each comprising a plurality of channels disposed through each layer. The systems can also include spacer arrays in contact with a perforated membrane to create a plurality of cell electroporation chambers for high-throughput cell electroporation.

A biological fluid processing device includes pump and valve systems, a controller, and an optical detection assembly. During a biological fluid processing procedure, the controller controls the operation of the pump and valve systems to separate a cell-containing fluid from the blood of a blood source and controls the optical detection assembly to emit light through the cell-containing fluid. The controller receives signals from the optical detection assembly that are indicative of the intensity of light that has passed through the cell-containing fluid. The controller receives or calculates an adjustment factor that is derived from historical data reflecting a cell collection procedure previously executed for the same blood source in which the same type of cell was collected. The controller determines an adjusted cell concentration of the cell-containing fluid based at least in part on the signals from the light detector and the adjustment factor.

The application discloses a fluorescent molecule positioning method, comprising the following steps: acquiring a single-frame original image of a living cell to be observed; denoising the single-frame original image; performing interpolation processing on the denoised single-frame original image according to a preset interpolation number; replacing the interpolated single-frame original image to obtain a new image; and reserving the largest local extreme point in the new image, which is the position of the fluorescent molecule. The method can realize real-time visual non-destructive super-resolution microscopic imaging of living cells and intracellular structures, and accurately determine the position of the fluorescent molecule.

Provided herein are, inter alia, methods for preparing a liquid cell culture media that has lesser lot-to-lot analytical variation, increased performance, and has lesser metal ion concentrations compared to a liquid media prepared by traditional methods. Such liquid media may be used for culturing cells, including but not limited to, recombinant cells.

The utility model provides an integrated organic element analyzer thermal conductivity detection cell body, which comprises a main body cell, a thermistor and a signal conditioning part, and the signal conditioning part is used for converting a resistance signal of the thermistor into a voltage or current signal; the device further comprises a data acquisition board and a driving part, the data acquisition board is arranged in the main body pool through a supporting column, the driving part provides voltage for the signal conditioning part to work, the thermistor, the signal conditioning part and the driving part are all fixed to the data acquisition board and electrically connected with the data acquisition board, and the data acquisition board is provided with an electrical interface. The driving component and the thermistor are integrated on one circuit board, so that the distance between the driving component and the thermistor is reduced, and coupling of external interference is reduced.

Presented herein is an electroporation shield configured to be removably coupled to an implantable stimulating assembly that includes one or more stimulation electrodes. The electroporation shield is coupled to stimulating assembly such that the electroporation shield electrically insulates the one or more of the stimulation electrodes from an electroporation electrical field generated by one or more electroporation electrodes coupled to the electroporation shield. The electroporation shield may be coupled to the stimulating assembly in a manner that facilitates removal of the electroporation shield and the one or more electroporation electrodes. After electroporation, without having to remove or reinsert the stimulating assembly, thereby exposing the one or more stimulation electrodes to the cells of the recipient for subsequent delivery of stimulation.

According to the present invention, light filters are disclosed that provide photoprotection and contain fluorescent agents in that have fluorescence emission in the near infrared region of wavelengths to effect photobiological modulation (PBM) of human cells when the cells are exposed to sunlight or any artificial lighting. The fluorescent agents include endogenous compounds. The light filters include ophthalmic lenses, skin care products with or without transparent substrate and any products that are used to filter light. When used in connection with ophthalmic lenses, the ophthalmic lense provides photoprotection and photobiological modulation to the human eye.

A cell characteristic detection device (10) is provided with a substrate (20) and a pair of resin supports (30) provided perpendicularly from the substrate (20) so as to be elastically deformable. The cell characteristic detection device (10) is capable of detecting the amount of displacement ([Delta] W) of the supports (30) from the outside in a state in which a cell aggregate (15) is held between the pair of supports (30) by the pair of supports (30).

The invention discloses an exosome cell extraction device applied to oral soluble film production, relates to the technical field of exosome extraction, improves the phenomenon that the time for workers to find an extraction head and a test tube is prolonged due to the fact that the extraction head and the test tube which are independently packaged are adopted for extraction, and comprises a box body and an upper cover, a placing mechanism is movably arranged on the inner bottom wall of the box body, an extracting mechanism is arranged on the placing mechanism, the extracting mechanism comprises a test tube, a sealing plug, an extracting assembly, a dismounting and mounting assembly and a stabilizing assembly which are arranged on the placing mechanism, and the sealing plug is installed at the top end of the test tube in a sealed mode; the extraction assembly is arranged on the sealing plug in a penetrating mode and extends into the test tube, the disassembly and assembly assemblies are arranged on the edges of the two sides of the top end of the test tube, the lower portion of the test tube is sleeved with the stabilizing assembly, the situation that the extraction head accidentally moves in the test tube can be avoided, and the extraction precision is improved.

The invention belongs to the technical field of cell viability on-line discrimination, and particularly relates to a mass spectrum-based cell viability measurement method and a mass spectrum-based cell viability measurement system. The invention provides a label-free endogenous metabolite-based cell viability measurement scheme and a label-free endogenous metabolite-based cell viability measurement system, aiming at the defects that the prior art depends on an exogenous label and cannot be synchronously carried out on line with single cell mass spectrometry. The method comprises the following steps: acquiring continuous single cell metabolome data through single cell electrospray ionization mass spectrometry, identifying a single cell event and a time window by taking ubiquitous membrane lipid as a first characteristic ion, extracting an endogenous metabolite reflecting cell activity in the window as a second characteristic ion, and calculating a signal-to-noise ratio; generating an activity label according to a preset threshold value and counting the cell viability of the group, wherein the system correspondingly comprises a data acquisition module, an event identification module, a marker extraction module, a threshold value judgment module and a viability counting module. According to the invention, synchronous, on-line and label-free accurate measurement of the cell viability in single cell metabonomics analysis is realized, and exogenous interference and data deviation are effectively avoided.

Cell analysis systems and instruments provide end users with time history graphics and imaging data of individual cell responses as caused by perturbations in the cell microenvironment and monitored by ChemFET sensors. The imaging data of the cellular response may be presented as an electron microscope image that is either an image taken from a sensor covering a cell footprint at a defined sampling interval of the cellular response or an image of the cellular response for a sensor detecting cellular effluents. Electron microscope imaging may be compared to optical imaging to provide information about additional dimensions of cellular analysis. For example, optical imaging using a known set of markers to compare immunocytochemical studies provides functional and phenotypic characteristics of data obtained for each cell analyzed using a ChemFET-based cellular analysis system.

The invention relates to the technical field of culture, and discloses an in-vitro cell culture electrical stimulation and recording device which comprises four quarter frames, the four quarter frames are mutually combined to form an integral frame, and a connection adjusting assembly capable of adjusting the distance between every two quarter frames is arranged between every two quarter frames. The recording electrode array comprises a 1 / 4 frame, the side surfaces of the two sides of the 1 / 4 frame are each provided with a first inlaying groove, a stimulating electrode is detachably inlaid in each first inlaying groove, and a first fixing assembly used for fixing the stimulating electrode is arranged in each first inlaying groove. A standard plug-in type experiment unit is formed, a user only needs to put the whole device into a standard culture dish, complex electrode arrangement can be completed, and the experiment preparation process is greatly simplified.

This application provides a method, apparatus, device, and medium for detecting physiological state. The method includes: first, acquiring the potential signal and environmental parameters from the skin surface of the worker; then, processing the potential signal using a signal inversion algorithm to determine the cell ion concentration; next, extracting the characteristic frequencies of the environmental parameters if the cell ion concentration is within a preset threshold; further, calculating a dynamic equilibrium coefficient based on the cell ion concentration, potential signal, and characteristic frequencies; and finally, determining the worker's physiological state based on the dynamic equilibrium coefficient. This method enables early identification of latent fatigue at the cellular level, improving workplace safety.

A chip includes a first substrate, a second substrate coupled with the first substrate, and a membrane disposed between the first substrate and the second substrate. The first substrate has a first channel, a first trench, a second trench, and a third trench, and the second substrate has a second channel corresponding to the first channel. The first trench, the second trench, and the third trench are extended from the first channel, and each of the trench forms an opening on the edge of the first substrate. A microfluidic cell culture device includes the chip, an incubator, and a potentiostat. A method for culturing a cell includes providing the microfluidic cell culture device; and introducing the cell to the membrane.

According to some embodiments of the present disclosure, a medical image analysis method performed by a computing device is disclosed. The method may include: acquiring a first dataset including a medical image obtained by photographing the prosthesis, inserted into the body, by a first photographing device and a second dataset including a medical image obtained by photographing the prosthesis, inserted into the body, by a second photographing device, wherein the first dataset includes a medical image having a higher resolution than the second dataset; training an analysis model for determining cell type information of the prosthesis by using the first dataset; and validating the analysis model by using the first dataset and the second dataset.

The utility model belongs to cell preparation technical field, concretely relates to a kind of tissue disrupter for cell preparation, including cell disrupter body, amplitude rod is installed in cell disrupter body inner wall, cell disrupter body inner wall installs lifting platform, lifting platform transverse end surface abuts beaker, silica gel pad that beaker surface abuts limit ring inner wall is bonded, limit ring other side surface is connected with moving rod, moving rod surface is slidably connected in the sliding slot of the top of mounting box, mounting box inner wall surface is connected with limit rod, limit rod other side surface is connected with fixed plate, the utility model, by the mutual cooperation between limit ring, moving rod, mounting box, sliding slot, limit rod, fixed plate, dovetail block, spring and side block, the beaker placed on lifting platform is clamped and limited, to avoid its in the process of being broken by ultrasonic vibration driven displacement, cause the influence cell breakage processing uniformity and security.

The application discloses a kind of based on ex vivo cell cosmetic detection equipment, including detection mechanism, the inside of the detection mechanism is provided with sterilization mechanism, the detection mechanism includes shell, the top of the shell is equipped with two storage grooves, the inner wall bottom of two storage grooves is fixed and is penetrated with discharge pipe, the inner wall one side of the shell is fixed with first hollow block, the inside of the first hollow block is provided with first recessed block, the inner wall other side of the shell is installed electric telescopic handle, the telescopic end of the electric telescopic handle is fixed with connecting plate, the inside of the shell is fixed with rectangular plate.The application can double, multiple detection to cosmetic by setting detection mechanism, that is, it can improve the detection accuracy of heavy metal detection device in cosmetic, effectively improve the use efficiency of cosmetic detection device, and facilitate better ex vivo cell cosmetic experiment in later period.

An endoprosthesis may include an expandable framework including an anchoring portion and a body portion extending axially from the anchoring portion, the body portion having a plurality of body cells; and a polymeric cover disposed on at least a portion of the expandable framework. The anchoring portion includes a first transverse flange and a second transverse flange proximate the first transverse flange, the first and second transverse flanges being configured to secure the anchoring portion at an orifice of a body lumen. The body portion includes a window through a side of the body portion, the window occupying space equivalent to at least two of the plurality of body cells. The window is devoid of the polymeric cover and any other structure within a perimeter of the window.

The application discloses a fluorescence light sheet microscopic imaging device and method, and belongs to the technical field of microscopic imaging. The device is composed of a low-coherence light source, a fiber coupler, a first collimator, a second collimator, a first moving platform, a second moving platform, a light sheet, a lens, a plane array camera and a computer. The method comprises a light sheet generation process and a light sheet imaging process. The light sheet imaging process is repeated to realize light sheet imaging of each section of a sample by moving different target imaging planes of the sample to an equal light path plane. The application realizes light sheet fluorescence imaging based on short-coherence light source interference. The thickness of the light sheet is reduced to the coherence length by taking advantage of the short coherence length of the short-coherence light, the longitudinal resolution is greatly improved, a small aperture is not needed, the excitation light intensity, bleaching and phototoxicity can be reduced, and in-vivo cell imaging can be realized without affecting cell activity. The plane array camera is used to collect the fluorescence signal of the focal plane of the sample, and the application has the characteristic of fast fluorescence signal collection speed.

The present invention generally relates to methods of producing magnetically induced secretomes with enhanced potency and stability, the methods comprise inter alia, employing polyacrylic acid based polymers, preferably a carbomer, to form a collapsible cell-encapsulating scaffold and dispersing donor cells within said scaffold prior to induction via a directionally-specific pulsing electromagnetic field (PEMF), or dispersing said donor cells in a "collapsed" polyacrylic acid based polymer scaffold prior to the PEMF induction. Also provided are the improved conditioned media thereof, suitable for use in medical and commercial applications.

This invention discloses a three-dimensional single-disc confocal microscope, belonging to the field of focusing microscope technology. Along the excitation optical path, the following components are arranged sequentially: a light source, a first biconvex lens, a cornerstone prism retroreflector, a short-pass dichroic mirror, a second biconvex lens, a concave hole disk, a focusing turntable, a third biconvex lens, and a sample tray. Along the imaging optical path, the following components are arranged sequentially: a sample tray, a third biconvex lens, a focusing turntable, a concave hole disk, a second biconvex lens, a short-pass dichroic mirror, a fourth biconvex lens, and an imaging module. The first biconvex lens has a focal length of f3, and the second biconvex lens has a focal length of f1. The apex of the cornerstone prism retroreflector is located at the focal point of the first and second biconvex lenses. The concave hole disk includes an annular disk with multiple circular holes. A concave surface coaxial with the holes is formed near the opening of the second biconvex lens. This invention enables rapid three-dimensional imaging of living cell tissues.

The utility model relates to the technical field of micro-droplet sorting, and discloses a cell pumping mechanism and a droplet microfluidic sorter. The cell pumping mechanism provided by the utility model comprises a turncap, a first channel used for circulating cell suspension and a second channel used for circulating gas are formed on the turncap; the liquid containing assembly is used for containing cell suspension, and the liquid containing assembly is communicated with the first channel and the second channel respectively; the pneumatic connector is arranged on the turncap and communicated with the second channel, the pneumatic connector introduces gas into the liquid loading assembly through the second channel, and the cell suspension is pumped into the first channel; the uniform mixing assembly is in transmission connection with the turncap, and the turncap is rotated by the uniform mixing assembly. The liquid drop micro-fluidic sorter disclosed by the utility model comprises the cell pumping mechanism. The utility model solves the problem that the cells are easy to agglomerate and precipitate during pumping, and realizes the technical effect of uniformly conveying the cells.

The present invention belongs to the field of medical micro-nano equipment technology, and specifically relates to an integrated microfluidic chip system and method for mass production and enrichment of engineered extracellular vesicles. The present invention first provides an integrated microfluidic chip system, comprising a microfluidic chip body and pre-placed objects; the microfluidic chip body comprises a cover layer 100, a flow channel layer 200, a liquid reservoir layer 300, and a conductive glass layer 400; the flow channel layer 200, the liquid reservoir layer 300, and the conductive glass layer 400 are integrated together by irreversible bonding, and the cover layer 100 and the flow channel layer 200 are detachably combined; a plurality of reaction zones, micropores, and microchannels are provided on the flow channel layer, and each reaction zone is separately provided and not connected; the pre-placed objects comprise one or more of donor cells, working cells, extracellular vesicle adsorption reagents, or reagents for nano-electroporation, and are provided in the microfluidic chip body.

Provided herein are methods of enriching for photoreceptors and photoreceptor precursor cells from mixed cell populations. Such enriched populations of photoreceptors and photoreceptor precursor cells can be used as cell therapies for the treatment of retinal disorders.