36 results about "Body cell" patented technology

The invention relates to the technical field of nuclear reactor fuel element analysis, in particular to an irradiated zirconium alloy plasticity limit prediction method based on a crystal plasticity finite element, which comprises the following steps: establishing a three-dimensional polycrystalline representative body cell model conforming to the actual grain size of zirconium alloy; based on a crystal plasticity theoretical model, zirconium alloy material parameters are obtained through calculation; boundary conditions and load conditions are determined, finite element calculation is executed in combination with material parameters, and an element stiffness matrix of each increment step is output; assembling the unit stiffness matrix, and obtaining a macroscopic stress-strain matrix of the three-dimensional polycrystal cell model through a homogenization method; and substituting the macroscopic stress-strain matrix into the Rice theoretical criterion, traversing the macroscopic stress-strain matrix of each increment step, and determining the plastic strain meeting the judgment critical condition. According to the method, accurate finite element plastic limit analysis can be carried out on the irradiation material, and the plastic limit strain of the zirconium alloy under the irradiation condition can be accurately judged.

The present disclosure describes systems, devices, and methods for high-throughput electroporation of a donor cell. The systems can include a perforated membrane having one or more layers, each comprising a plurality of channels disposed through each layer. The systems can also include spacer arrays in contact with a perforated membrane to create a plurality of cell electroporation chambers for high-throughput cell electroporation.

A biological fluid processing device includes pump and valve systems, a controller, and an optical detection assembly. During a biological fluid processing procedure, the controller controls the operation of the pump and valve systems to separate a cell-containing fluid from the blood of a blood source and controls the optical detection assembly to emit light through the cell-containing fluid. The controller receives signals from the optical detection assembly that are indicative of the intensity of light that has passed through the cell-containing fluid. The controller receives or calculates an adjustment factor that is derived from historical data reflecting a cell collection procedure previously executed for the same blood source in which the same type of cell was collected. The controller determines an adjusted cell concentration of the cell-containing fluid based at least in part on the signals from the light detector and the adjustment factor.

The application discloses a fluorescent molecule positioning method, comprising the following steps: acquiring a single-frame original image of a living cell to be observed; denoising the single-frame original image; performing interpolation processing on the denoised single-frame original image according to a preset interpolation number; replacing the interpolated single-frame original image to obtain a new image; and reserving the largest local extreme point in the new image, which is the position of the fluorescent molecule. The method can realize real-time visual non-destructive super-resolution microscopic imaging of living cells and intracellular structures, and accurately determine the position of the fluorescent molecule.

Provided herein are, inter alia, methods for preparing a liquid cell culture media that has lesser lot-to-lot analytical variation, increased performance, and has lesser metal ion concentrations compared to a liquid media prepared by traditional methods. Such liquid media may be used for culturing cells, including but not limited to, recombinant cells.

The utility model provides an integrated organic element analyzer thermal conductivity detection cell body, which comprises a main body cell, a thermistor and a signal conditioning part, and the signal conditioning part is used for converting a resistance signal of the thermistor into a voltage or current signal; the device further comprises a data acquisition board and a driving part, the data acquisition board is arranged in the main body pool through a supporting column, the driving part provides voltage for the signal conditioning part to work, the thermistor, the signal conditioning part and the driving part are all fixed to the data acquisition board and electrically connected with the data acquisition board, and the data acquisition board is provided with an electrical interface. The driving component and the thermistor are integrated on one circuit board, so that the distance between the driving component and the thermistor is reduced, and coupling of external interference is reduced.

Presented herein is an electroporation shield configured to be removably coupled to an implantable stimulating assembly that includes one or more stimulation electrodes. The electroporation shield is coupled to stimulating assembly such that the electroporation shield electrically insulates the one or more of the stimulation electrodes from an electroporation electrical field generated by one or more electroporation electrodes coupled to the electroporation shield. The electroporation shield may be coupled to the stimulating assembly in a manner that facilitates removal of the electroporation shield and the one or more electroporation electrodes. After electroporation, without having to remove or reinsert the stimulating assembly, thereby exposing the one or more stimulation electrodes to the cells of the recipient for subsequent delivery of stimulation.

According to the present invention, light filters are disclosed that provide photoprotection and contain fluorescent agents in that have fluorescence emission in the near infrared region of wavelengths to effect photobiological modulation (PBM) of human cells when the cells are exposed to sunlight or any artificial lighting. The fluorescent agents include endogenous compounds. The light filters include ophthalmic lenses, skin care products with or without transparent substrate and any products that are used to filter light. When used in connection with ophthalmic lenses, the ophthalmic lense provides photoprotection and photobiological modulation to the human eye.

The invention belongs to the technical field of cell viability on-line discrimination, and particularly relates to a mass spectrum-based cell viability measurement method and a mass spectrum-based cell viability measurement system. The invention provides a label-free endogenous metabolite-based cell viability measurement scheme and a label-free endogenous metabolite-based cell viability measurement system, aiming at the defects that the prior art depends on an exogenous label and cannot be synchronously carried out on line with single cell mass spectrometry. The method comprises the following steps: acquiring continuous single cell metabolome data through single cell electrospray ionization mass spectrometry, identifying a single cell event and a time window by taking ubiquitous membrane lipid as a first characteristic ion, extracting an endogenous metabolite reflecting cell activity in the window as a second characteristic ion, and calculating a signal-to-noise ratio; generating an activity label according to a preset threshold value and counting the cell viability of the group, wherein the system correspondingly comprises a data acquisition module, an event identification module, a marker extraction module, a threshold value judgment module and a viability counting module. According to the invention, synchronous, on-line and label-free accurate measurement of the cell viability in single cell metabonomics analysis is realized, and exogenous interference and data deviation are effectively avoided.

Cell analysis systems and instruments provide end users with time history graphics and imaging data of individual cell responses as caused by perturbations in the cell microenvironment and monitored by ChemFET sensors. The imaging data of the cellular response may be presented as an electron microscope image that is either an image taken from a sensor covering a cell footprint at a defined sampling interval of the cellular response or an image of the cellular response for a sensor detecting cellular effluents. Electron microscope imaging may be compared to optical imaging to provide information about additional dimensions of cellular analysis. For example, optical imaging using a known set of markers to compare immunocytochemical studies provides functional and phenotypic characteristics of data obtained for each cell analyzed using a ChemFET-based cellular analysis system.

The invention relates to the technical field of culture, and discloses an in-vitro cell culture electrical stimulation and recording device which comprises four quarter frames, the four quarter frames are mutually combined to form an integral frame, and a connection adjusting assembly capable of adjusting the distance between every two quarter frames is arranged between every two quarter frames. The recording electrode array comprises a 1 / 4 frame, the side surfaces of the two sides of the 1 / 4 frame are each provided with a first inlaying groove, a stimulating electrode is detachably inlaid in each first inlaying groove, and a first fixing assembly used for fixing the stimulating electrode is arranged in each first inlaying groove. A standard plug-in type experiment unit is formed, a user only needs to put the whole device into a standard culture dish, complex electrode arrangement can be completed, and the experiment preparation process is greatly simplified.

This application provides a method, apparatus, device, and medium for detecting physiological state. The method includes: first, acquiring the potential signal and environmental parameters from the skin surface of the worker; then, processing the potential signal using a signal inversion algorithm to determine the cell ion concentration; next, extracting the characteristic frequencies of the environmental parameters if the cell ion concentration is within a preset threshold; further, calculating a dynamic equilibrium coefficient based on the cell ion concentration, potential signal, and characteristic frequencies; and finally, determining the worker's physiological state based on the dynamic equilibrium coefficient. This method enables early identification of latent fatigue at the cellular level, improving workplace safety.

According to some embodiments of the present disclosure, a medical image analysis method performed by a computing device is disclosed. The method may include: acquiring a first dataset including a medical image obtained by photographing the prosthesis, inserted into the body, by a first photographing device and a second dataset including a medical image obtained by photographing the prosthesis, inserted into the body, by a second photographing device, wherein the first dataset includes a medical image having a higher resolution than the second dataset; training an analysis model for determining cell type information of the prosthesis by using the first dataset; and validating the analysis model by using the first dataset and the second dataset.

The utility model belongs to cell preparation technical field, concretely relates to a kind of tissue disrupter for cell preparation, including cell disrupter body, amplitude rod is installed in cell disrupter body inner wall, cell disrupter body inner wall installs lifting platform, lifting platform transverse end surface abuts beaker, silica gel pad that beaker surface abuts limit ring inner wall is bonded, limit ring other side surface is connected with moving rod, moving rod surface is slidably connected in the sliding slot of the top of mounting box, mounting box inner wall surface is connected with limit rod, limit rod other side surface is connected with fixed plate, the utility model, by the mutual cooperation between limit ring, moving rod, mounting box, sliding slot, limit rod, fixed plate, dovetail block, spring and side block, the beaker placed on lifting platform is clamped and limited, to avoid its in the process of being broken by ultrasonic vibration driven displacement, cause the influence cell breakage processing uniformity and security.

The application discloses a kind of based on ex vivo cell cosmetic detection equipment, including detection mechanism, the inside of the detection mechanism is provided with sterilization mechanism, the detection mechanism includes shell, the top of the shell is equipped with two storage grooves, the inner wall bottom of two storage grooves is fixed and is penetrated with discharge pipe, the inner wall one side of the shell is fixed with first hollow block, the inside of the first hollow block is provided with first recessed block, the inner wall other side of the shell is installed electric telescopic handle, the telescopic end of the electric telescopic handle is fixed with connecting plate, the inside of the shell is fixed with rectangular plate.The application can double, multiple detection to cosmetic by setting detection mechanism, that is, it can improve the detection accuracy of heavy metal detection device in cosmetic, effectively improve the use efficiency of cosmetic detection device, and facilitate better ex vivo cell cosmetic experiment in later period.

An endoprosthesis may include an expandable framework including an anchoring portion and a body portion extending axially from the anchoring portion, the body portion having a plurality of body cells; and a polymeric cover disposed on at least a portion of the expandable framework. The anchoring portion includes a first transverse flange and a second transverse flange proximate the first transverse flange, the first and second transverse flanges being configured to secure the anchoring portion at an orifice of a body lumen. The body portion includes a window through a side of the body portion, the window occupying space equivalent to at least two of the plurality of body cells. The window is devoid of the polymeric cover and any other structure within a perimeter of the window.

The application discloses a fluorescence light sheet microscopic imaging device and method, and belongs to the technical field of microscopic imaging. The device is composed of a low-coherence light source, a fiber coupler, a first collimator, a second collimator, a first moving platform, a second moving platform, a light sheet, a lens, a plane array camera and a computer. The method comprises a light sheet generation process and a light sheet imaging process. The light sheet imaging process is repeated to realize light sheet imaging of each section of a sample by moving different target imaging planes of the sample to an equal light path plane. The application realizes light sheet fluorescence imaging based on short-coherence light source interference. The thickness of the light sheet is reduced to the coherence length by taking advantage of the short coherence length of the short-coherence light, the longitudinal resolution is greatly improved, a small aperture is not needed, the excitation light intensity, bleaching and phototoxicity can be reduced, and in-vivo cell imaging can be realized without affecting cell activity. The plane array camera is used to collect the fluorescence signal of the focal plane of the sample, and the application has the characteristic of fast fluorescence signal collection speed.

The utility model relates to the technical field of micro-droplet sorting, and discloses a cell pumping mechanism and a droplet microfluidic sorter. The cell pumping mechanism provided by the utility model comprises a turncap, a first channel used for circulating cell suspension and a second channel used for circulating gas are formed on the turncap; the liquid containing assembly is used for containing cell suspension, and the liquid containing assembly is communicated with the first channel and the second channel respectively; the pneumatic connector is arranged on the turncap and communicated with the second channel, the pneumatic connector introduces gas into the liquid loading assembly through the second channel, and the cell suspension is pumped into the first channel; the uniform mixing assembly is in transmission connection with the turncap, and the turncap is rotated by the uniform mixing assembly. The liquid drop micro-fluidic sorter disclosed by the utility model comprises the cell pumping mechanism. The utility model solves the problem that the cells are easy to agglomerate and precipitate during pumping, and realizes the technical effect of uniformly conveying the cells.

Provided herein are methods of enriching for photoreceptors and photoreceptor precursor cells from mixed cell populations. Such enriched populations of photoreceptors and photoreceptor precursor cells can be used as cell therapies for the treatment of retinal disorders.

The utility model discloses a control device based on a cell lysis process, which comprises an equipment main body, a cell suspension supply module, a lysis solution supply module, a neutralizing solution supply module, a first mixing point and a second mixing point, and a mixed solution outlet pipeline is connected between the first mixing point and the second mixing point; at least one of the cell suspension supply pipeline, the lysis solution supply pipeline and the neutralizing solution supply pipeline is fixedly connected with the equipment main body; the system further comprises a first flow detection element, a second flow detection element and a third flow detection element, the first flow detection element is arranged on the cell suspension supply pipeline, the second flow detection element is arranged on the lysis solution supply pipeline, and the third flow detection element is arranged on the mixed solution outlet pipeline; the inert replenishing liquid supply module is connected with the cell suspension supply pipeline and is used for supplying inert replenishing liquid to the cell suspension supply pipeline, so that the cell suspension is completely consumed as much as possible, and loss is avoided.

The application discloses a centrifugal tube and assembly for making cell wax blocks. The centrifugal tube comprises a ball-sac-equipped inner thread top cover, a centrifugal tube body composed of a funnel part and a cell enrichment tube, a cell collection capsule, a bottom pad and a bottom-sealed inner thread cover. The cell collection capsule and the bottom pad are arranged inside the cell enrichment tube to collect cells and can be taken in and out of the lower opening of the cell enrichment tube. After installation, detection liquid is added to the centrifugal tube body, and the centrifugal tube body is centrifuged by a centrifugal machine. The cells in the liquid are settled in the collection capsule. The bottom thread cover is unscrewed, the ball sac is pressed, and the cell collection capsule with the internal cell sediment is discharged from the lower opening of the cell enrichment tube under the hydraulic action. Then, the cell collection capsule is taken with the capsule cap and is placed in 10% neutral formalin solution for fixation. The fixing solution gradually penetrates into the cell mass, and the cell sediment gradually forms a certain shape and hardness. In this way, the cell wax block is simple and convenient to make and is not limited by the small amount of cells.

The invention discloses a sampling brush for collecting human cells. Comprising a brush rod with a hollow cavity; the brush head is arranged at one end of the brush rod; the telescopic structure is arranged to penetrate through the hollow cavity and is used for enabling the brush head to retract into the cavity after cell collection is completed; wherein the telescopic structure comprises a translation piece and a rotating piece, one end of the translation piece is fixedly connected with the brush head, and the other end of the translation piece is rotatably connected with the rotating piece. The problem of how to prevent the brush head from being polluted and collided after cell collection is completed can be solved.

1. The name of the design product: health cabin. 2. The use of the design product: for body cell and nerve conditioning, health therapy. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

Induction of overexpression of defined exogenous transcription factors (TFs) or treatment with specific pathway-modulating cocktails can reprogram somatic cells to pluripotency or another cell state. A barrier to initiating reprogramming lies in the molecular identity of the starting cell, which is enforced by lineage-directing TFs. However, it remains unclear whether suppression of somatic lineage-defining TFs in the starting cell in the absence of such exogenous TFs is sufficient to induce cellular reprogramming. Using an intraspecific somatic cell hybrid model, we identify SNAI2 and PRRX1 as the most important determinants of mesenchymal commitment in rat fetal fibroblasts (REFs) and demonstrate that siRNA-mediated transient knockdown of these individual factors is sufficient to convert REFs into functional adipocytes, chondrocytes, or osteocytes without the need for exogenous TFs. In addition, siRNA-mediated transient knockdown of SNAI2 alone is sufficient to transform REFs into a dedifferentiated pluripotent stem-like cell (dPSC) state that forms embryoid bodies in the absence of exogenous TFs, demonstrating that triple germ layer differentiation is possible. These results establish for the first time that transient inhibition of a single somatic lineage-specifying TF can effectively induce transdifferentiation to another somatic state or dedifferentiation to dPSCs in the absence of exogenous TFs or small molecule cocktails.

The invention relates to the technical field of material surface modification precision machining, and provides a preparation method of a femtosecond laser-induced implant electric signal shielding microstructure, a biological metal substrate material is completely immersed in a transparent liquid, and the transparent liquid is an HEPES buffer solution filled with inert gas. A stable microbubble array is formed through ultrasonic treatment induction, femtosecond laser is focused on an area above a material, and path scanning operation is carried out. And obtaining a dual-scale structure formed by the central microcavity and the nano texture on the surface of the material. According to the method, a femtosecond laser processing technology can be utilized, and a transient nonlinear light-induced breakdown effect of the femtosecond laser processing technology in a liquid phase is combined, so that a local microcavity and nano-grain composite dual-scale structure is induced to be formed, and charge distribution and dielectric properties on the surface of a material are accurately regulated and controlled. The invention further discloses an electric signal shielding microstructure and antibacterial application, and the double-scale structure not only has the function of regulating and controlling behaviors of adult cells, but also can effectively shield or interfere the electric signal conduction process between bacteria.

This invention relates to a microscopic scanning focusing method and system adapted for live cell slides. By establishing a focal plane height model reflecting the spatial mapping relationship between the plane coordinates of the microgroove and the focal height at the bottom of the groove, the traditional large-scale focusing search starting from zero point by point is simplified to a local search guided by predicted focal height, significantly reducing the focusing time for each microgroove. Within the local search area, the sharpness characteristics and cell texture characteristics of the microscopic image are combined to distinguish between the false focal peaks generated by the liquid-gas interface and the true focal peaks of the cell layer at the bottom of the groove, so that the focusing results are not affected by the reflection from the transparent culture medium surface, ensuring that the focus is always locked on the cell layer at the bottom of the groove rather than the liquid surface. Based on this, the light source is triggered for short-time exposure imaging only when the objective lens and stage reach the focal height of the cell layer at the bottom of the groove determined after eliminating false focal peaks. Thus, while taking into account scanning efficiency, the experimental repeatability and data reliability of long-term serial observation of live cells are guaranteed.

The invention provides a dual-view collaborative living cell optimization method and equipment. The method comprises the following steps: calibrating a first view and a second view; performing activity evaluation on living cells in the culture dish under a first visual field, and screening first target living cells; adjusting the position of the culture dish, and moving the first target living cells into a second visual field; in a second visual field, performing form evaluation on the first target living cells, and screening out second target living cells; the position of the culture dish is adjusted, and the second target living cells are fixed through the laser spots. Through double-view calibration, the alignment precision of a laser spot and a living cell is improved, and the living cell is prevented from deviating from the laser spot, so that the living cell is prevented from struggling off due to infirm fixation, and the living cell is prevented from being damaged, low in grabbing efficiency and the like; living cells are moved into a second visual field by adjusting a culture dish, so that the form evaluation precision is improved; and through double screening of vitality and form, the misjudgment risk of a single evaluation dimension is reduced, and the reliability of a screening result is improved.

The invention relates to a 3D printing device and method based on rotating X-rays, and the printing device comprises an X-ray generation module which is used for outputting an X-ray beam; the rotary resin container system is used for containing an X-ray curable material and rotating around the central axis of the rotary resin container system; the X-ray mask assembly is used for shaping the X-ray beam into a two-dimensional projection pattern corresponding to the rotation angle of the rotary resin container system, so that the two-dimensional projection pattern sequentially accumulates doses in different circumferential areas of the X-ray curable material; the dose monitoring module is used for collecting the X-ray dose after the X-ray curing material passes through the X-ray curing material in real time and outputting a dose feedback signal; the control system is used for adjusting the power and / or irradiation time of the X-ray source in real time until the accumulated dose of the target area reaches a threshold value, so that the X-ray curable material is cured into a three-dimensional object, and the adaptation range of the material can be greatly widened; the forming quality and the size precision of the part are improved, the biocompatibility is improved, and printing of the biomedical implant containing living cells can be achieved.

The application discloses a water-soluble cationic fluorescent probe dye and application thereof in cell membrane staining. The fluorescent probe has good water solubility, can be used in in-vivo cell fluorescence imaging, realizes rapid and efficient staining of cell plasma membranes, and does not need to be cleaned. The fluorescent probe has an orange red fluorescence color, has a large Stokes shift, and has strong anti-self-fluorescence interference capability.