85 results about "Body cell" patented technology

The invention relates to the technical field of nuclear reactor fuel element analysis, in particular to an irradiated zirconium alloy plasticity limit prediction method based on a crystal plasticity finite element, which comprises the following steps: establishing a three-dimensional polycrystalline representative body cell model conforming to the actual grain size of zirconium alloy; based on a crystal plasticity theoretical model, zirconium alloy material parameters are obtained through calculation; boundary conditions and load conditions are determined, finite element calculation is executed in combination with material parameters, and an element stiffness matrix of each increment step is output; assembling the unit stiffness matrix, and obtaining a macroscopic stress-strain matrix of the three-dimensional polycrystal cell model through a homogenization method; and substituting the macroscopic stress-strain matrix into the Rice theoretical criterion, traversing the macroscopic stress-strain matrix of each increment step, and determining the plastic strain meeting the judgment critical condition. According to the method, accurate finite element plastic limit analysis can be carried out on the irradiation material, and the plastic limit strain of the zirconium alloy under the irradiation condition can be accurately judged.

The application relates to a kind of gland cell image segmentation methods based on selective multi-branch hollow convolution, input gland cell image dataset, with improved UNet as main network, construct gland cell segmentation network based on selective multi-branch hollow convolution, the network is captured more advanced features with context encoding network, with channel attention module adaptive recalibration channel feature response and highlight the most relevant feature channel, with multi-scale selection module amplifies effective information and suppresses redundant information, selects the appropriate scale of hollow convolution branch;With the input gland cell image dataset, the segmentation network is trained to obtain a stable segmentation network;Input to be segmented gland cell image, with stable segmentation network to carry out gland cell segmentation.The application has better generalization ability, and is especially suitable for the size, shape change difference of gland in colorectal polyp.

The invention discloses an optical fiber micro-beam probe and system capable of achieving synchronous imaging of cell morphology and mechanical characteristics, the optical fiber micro-beam probe comprises a single-mode optical fiber, a Bragg grating, a supporting column, a micro-beam and a nanometer needle tip, the single-mode optical fiber comprises a fiber core and a wrapping layer outside the fiber core, and the Bragg grating is located in the fiber core of the single-mode optical fiber; the first end of the supporting column is fixed to the second end face of the single-mode optical fiber, one end of the micro-beam is fixed to the second end of the supporting column, and the nanometer needle tip is located on the surface of the other end of the micro-beam. The metal film is introduced to enhance the reflectivity of the micro-beam, and the nano needle tip is introduced to improve the cell morphology and mechanical imaging resolution. An optical fiber FPI sensitive external weak force is formed on the end face of an optical fiber by adopting a micro-beam probe, resonance of the micro-beam probe is excited by light driving, the resonance is influenced by the weak attractive force on the surface of a cell, the change of information such as resonant frequency and amplitude is monitored, and synchronous imaging of the morphology and the mechanical property of the living cell is realized. Miniaturization, integration and high efficiency of an imaging system are facilitated, and a new method is provided for cell imaging in the fields of biomedicine and the like.

The utility model discloses a cell slide culture device, relates to cell culture technical field, including box body, cell slide, slide groove, upper cover and screw, said box body is provided with a plurality of slide groove, said slide groove is placed with cell slide, said box body both sides is provided with a plurality of threaded hole, said upper cover is equipped with the screw, said upper cover is equipped with the screw, said upper cover is equipped with the screw, said upper cover is equipped with the screw. A clamping groove is formed in the edge of the top end of the box body in a surrounding mode, the upper cover is integrally embedded into the clamping groove, and the screws penetrate through the upper cover to be matched with the threaded holes. The cell climbing piece is simple to operate, convenient to use and easy to take and place from the box body, the situation that cells fall off from the cell climbing piece is reduced, and an operator can conveniently carry the cell climbing piece outside a laboratory.

Provided herein are, inter alia, methods for preparing a liquid cell culture media that has lesser lot-to-lot analytical variation, increased performance, and has lesser metal ion concentrations compared to a liquid media prepared by traditional methods. Such liquid media may be used for culturing cells, including but not limited to, recombinant cells.

The embodiment of the specification relates to the technical field of super-resolution fluorescence microscopy, and particularly relates to an optical superlens optical sheet endoscopy imaging system and method. Phase control is performed on short-wavelength laser by an optical sheet superlens, so that incident light on a sample is finely adjusted into the form of an optical sheet, the short-wavelength laser after phase control is irradiated on the sample, the optical sheet and the sample interact to excite fluorescence, at the same time, phase control is performed on long-wavelength laser by an OAM light superlens, the obtained OAM light is irradiated on the sample, the OAM light quenches fluorescent substances at a specified position in a fluorescent spot excited by the short-wavelength laser, so that the diffraction area of the fluorescent spot is reduced, finally, fluorescence remaining after OAM light irradiation of the sample is converged on a photodiode for fluorescence power detection, and the resolution of imaging is improved on the basis of scanning of samples such as living cells.

The present disclosure describes systems, devices, and methods for high-throughput electroporation of a donor cell. The systems can include a perforated membrane having one or more layers, each comprising a plurality of channels disposed through each layer. The systems can also include spacer arrays in contact with a perforated membrane to create a plurality of cell electroporation chambers for high-throughput cell electroporation.

A biological fluid processing device includes pump and valve systems, a controller, and an optical detection assembly. During a biological fluid processing procedure, the controller controls the operation of the pump and valve systems to separate a cell-containing fluid from the blood of a blood source and controls the optical detection assembly to emit light through the cell-containing fluid. The controller receives signals from the optical detection assembly that are indicative of the intensity of light that has passed through the cell-containing fluid. The controller receives or calculates an adjustment factor that is derived from historical data reflecting a cell collection procedure previously executed for the same blood source in which the same type of cell was collected. The controller determines an adjusted cell concentration of the cell-containing fluid based at least in part on the signals from the light detector and the adjustment factor.

1. The name of this design product: 3D living cell microscope. 2. Purpose of the product of this design: used for real-time imaging of label-free 3D morphology of cells. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The application discloses a fluorescent molecule positioning method, comprising the following steps: acquiring a single-frame original image of a living cell to be observed; denoising the single-frame original image; performing interpolation processing on the denoised single-frame original image according to a preset interpolation number; replacing the interpolated single-frame original image to obtain a new image; and reserving the largest local extreme point in the new image, which is the position of the fluorescent molecule. The method can realize real-time visual non-destructive super-resolution microscopic imaging of living cells and intracellular structures, and accurately determine the position of the fluorescent molecule.

Embodiments are disclosed for a system. The system includes multiple tracks. Further, one track includes a power rail for a first voltage. The system also includes a first via, disposed beneath, and in electrical contact with, the power rail. The system additionally includes a first contact, beneath, and in electrical contact with, the first via. The system further includes a first field effect transistor (FET), beneath, and in electrical isolation with, the first contact. Additionally, the system includes a second FET, beneath, and in electrical contact with, the first FET. Further, the system includes a second contact, beneath, and in electrical contact with, the second FET. Also, the system includes a second via, beneath, and in electrical contact with, the second contact. The system additionally includes a buried power rail (BPR), beneath, and in electrical contact with, the second via, wherein the BPR comprises a second voltage.

A prosthetic liner configured to be worn over a residual limb of a user is disclosed. The disclosed prosthetic liner can include a gel sleeve configured to be worn over the residual limb and comprising a plurality of cells disposed on the gel sleeve configured to receive a fluid and exert pressure on the residual limb, a pump fluidically coupled to the gel sleeve and configured to move the fluid into the plurality of cells, and a control board electrically coupled to both the gel sleeve and the pump and configured to control activation of the pump.

Provided herein are, inter alia, methods for preparing a liquid cell culture media that has lesser lot-to-lot analytical variation, increased performance, and has lesser metal ion concentrations compared to a liquid media prepared by traditional methods. Such liquid media may be used for culturing cells, including but not limited to, recombinant cells.

The utility model provides an integrated organic element analyzer thermal conductivity detection cell body, which comprises a main body cell, a thermistor and a signal conditioning part, and the signal conditioning part is used for converting a resistance signal of the thermistor into a voltage or current signal; the device further comprises a data acquisition board and a driving part, the data acquisition board is arranged in the main body pool through a supporting column, the driving part provides voltage for the signal conditioning part to work, the thermistor, the signal conditioning part and the driving part are all fixed to the data acquisition board and electrically connected with the data acquisition board, and the data acquisition board is provided with an electrical interface. The driving component and the thermistor are integrated on one circuit board, so that the distance between the driving component and the thermistor is reduced, and coupling of external interference is reduced.

Light-transmitting material with filter properties to provide an environment suited to the visual characteristics of the user In addition, it is possible to provide a filter having filter characteristics that take into account the characteristics of human cone cells. It is possible to provide a light transmitting material. The light transmitting material is a light transmitting material coated with a coating agent having a predetermined filter characteristic. A filter material, the predetermined filter characteristics being a transmittance of 60% in the range of 400 to 480 nm. and a relationship between the predetermined filter characteristic and the absorption spectrum of human blue cone cells of 40 The convolution integral in the wavelength range of 0 to 480 nm is the standard filter characteristic with a transmittance of 100%. The characteristic of being cut by 40% or more from the convolution integral of the relevant absorption spectrum in the relevant wavelength band. It is sexuality.

[Summary] The present application relates to an optical device, comprising: a first outer substrate; a liquid crystal cell and a second outer substrate sequentially, wherein the liquid crystal cell co mprises an upper substrate including a first base layer and a pressure-sensitive adhesive layer; a lower substrate including a second base layer and spacers; and a liquid crystal layer includin g a liquid crystal compound between the upper substrate and the lower substrate, and the optical device further comprises: a first intermediate layer between the first outer substrate and the liq uid crystal cell, and a second intermediate layer between the second outer substrate and the liq uid crystal cell, wherein the optical device of the present application can properly maintain a cell gap of a liquid crystal cell, have excellent adhesion between an upper substrate and a lower substrate, and solve the pressing and overflow phenomena of the liquid crystal cell during bonding with an outer substrate.

Presented herein is an electroporation shield configured to be removably coupled to an implantable stimulating assembly that includes one or more stimulation electrodes. The electroporation shield is coupled to stimulating assembly such that the electroporation shield electrically insulates the one or more of the stimulation electrodes from an electroporation electrical field generated by one or more electroporation electrodes coupled to the electroporation shield. The electroporation shield may be coupled to the stimulating assembly in a manner that facilitates removal of the electroporation shield and the one or more electroporation electrodes. After electroporation, without having to remove or reinsert the stimulating assembly, thereby exposing the one or more stimulation electrodes to the cells of the recipient for subsequent delivery of stimulation.

According to the present invention, light filters are disclosed that provide photoprotection and contain fluorescent agents in that have fluorescence emission in the near infrared region of wavelengths to effect photobiological modulation (PBM) of human cells when the cells are exposed to sunlight or any artificial lighting. The fluorescent agents include endogenous compounds. The light filters include ophthalmic lenses, skin care products with or without transparent substrate and any products that are used to filter light. When used in connection with ophthalmic lenses, the ophthalmic lense provides photoprotection and photobiological modulation to the human eye.

A thin film liquid cell suitable for transmission electron microscopy at room temperature is fabricated as follows. A thin film floating on a liquid is prepared. A droplet of the liquid with the thin film floating thereon is transferred to a support by means of a loop. The loop carries the droplet and the droplet carries the thin film during this transfer. Sufficient liquid from the droplet on the support is removed to form the thin film liquid cell.

A cell characteristic detection device (10) is provided with a substrate (20) and a pair of resin supports (30) provided perpendicularly from the substrate (20) so as to be elastically deformable. The cell characteristic detection device (10) is capable of detecting the amount of displacement ([Delta] W) of the supports (30) from the outside in a state in which a cell aggregate (15) is held between the pair of supports (30) by the pair of supports (30).

The invention discloses an exosome cell extraction device applied to oral soluble film production, relates to the technical field of exosome extraction, improves the phenomenon that the time for workers to find an extraction head and a test tube is prolonged due to the fact that the extraction head and the test tube which are independently packaged are adopted for extraction, and comprises a box body and an upper cover, a placing mechanism is movably arranged on the inner bottom wall of the box body, an extracting mechanism is arranged on the placing mechanism, the extracting mechanism comprises a test tube, a sealing plug, an extracting assembly, a dismounting and mounting assembly and a stabilizing assembly which are arranged on the placing mechanism, and the sealing plug is installed at the top end of the test tube in a sealed mode; the extraction assembly is arranged on the sealing plug in a penetrating mode and extends into the test tube, the disassembly and assembly assemblies are arranged on the edges of the two sides of the top end of the test tube, the lower portion of the test tube is sleeved with the stabilizing assembly, the situation that the extraction head accidentally moves in the test tube can be avoided, and the extraction precision is improved.

The invention belongs to the technical field of cell viability on-line discrimination, and particularly relates to a mass spectrum-based cell viability measurement method and a mass spectrum-based cell viability measurement system. The invention provides a label-free endogenous metabolite-based cell viability measurement scheme and a label-free endogenous metabolite-based cell viability measurement system, aiming at the defects that the prior art depends on an exogenous label and cannot be synchronously carried out on line with single cell mass spectrometry. The method comprises the following steps: acquiring continuous single cell metabolome data through single cell electrospray ionization mass spectrometry, identifying a single cell event and a time window by taking ubiquitous membrane lipid as a first characteristic ion, extracting an endogenous metabolite reflecting cell activity in the window as a second characteristic ion, and calculating a signal-to-noise ratio; generating an activity label according to a preset threshold value and counting the cell viability of the group, wherein the system correspondingly comprises a data acquisition module, an event identification module, a marker extraction module, a threshold value judgment module and a viability counting module. According to the invention, synchronous, on-line and label-free accurate measurement of the cell viability in single cell metabonomics analysis is realized, and exogenous interference and data deviation are effectively avoided.

Cell analysis systems and instruments provide end users with time history graphics and imaging data of individual cell responses as caused by perturbations in the cell microenvironment and monitored by ChemFET sensors. The imaging data of the cellular response may be presented as an electron microscope image that is either an image taken from a sensor covering a cell footprint at a defined sampling interval of the cellular response or an image of the cellular response for a sensor detecting cellular effluents. Electron microscope imaging may be compared to optical imaging to provide information about additional dimensions of cellular analysis. For example, optical imaging using a known set of markers to compare immunocytochemical studies provides functional and phenotypic characteristics of data obtained for each cell analyzed using a ChemFET-based cellular analysis system.

The invention relates to the technical field of culture, and discloses an in-vitro cell culture electrical stimulation and recording device which comprises four quarter frames, the four quarter frames are mutually combined to form an integral frame, and a connection adjusting assembly capable of adjusting the distance between every two quarter frames is arranged between every two quarter frames. The recording electrode array comprises a 1 / 4 frame, the side surfaces of the two sides of the 1 / 4 frame are each provided with a first inlaying groove, a stimulating electrode is detachably inlaid in each first inlaying groove, and a first fixing assembly used for fixing the stimulating electrode is arranged in each first inlaying groove. A standard plug-in type experiment unit is formed, a user only needs to put the whole device into a standard culture dish, complex electrode arrangement can be completed, and the experiment preparation process is greatly simplified.

This application provides a method, apparatus, device, and medium for detecting physiological state. The method includes: first, acquiring the potential signal and environmental parameters from the skin surface of the worker; then, processing the potential signal using a signal inversion algorithm to determine the cell ion concentration; next, extracting the characteristic frequencies of the environmental parameters if the cell ion concentration is within a preset threshold; further, calculating a dynamic equilibrium coefficient based on the cell ion concentration, potential signal, and characteristic frequencies; and finally, determining the worker's physiological state based on the dynamic equilibrium coefficient. This method enables early identification of latent fatigue at the cellular level, improving workplace safety.

A chip includes a first substrate, a second substrate coupled with the first substrate, and a membrane disposed between the first substrate and the second substrate. The first substrate has a first channel, a first trench, a second trench, and a third trench, and the second substrate has a second channel corresponding to the first channel. The first trench, the second trench, and the third trench are extended from the first channel, and each of the trench forms an opening on the edge of the first substrate. A microfluidic cell culture device includes the chip, an incubator, and a potentiostat. A method for culturing a cell includes providing the microfluidic cell culture device; and introducing the cell to the membrane.

The invention discloses a micro-fluidic chip for enriching exosomes and a preparation method of the micro-fluidic chip, and relates to the field of extraction of exosome cells. The micro-fluidic chip comprises an upper-layer chip, a lower-layer chip and a slide which are sequentially arranged from top to bottom. The upper-layer chip is provided with a first cavity with a downward opening, the lower-layer chip is provided with a vertically-through second cavity, and the first cavity is communicated with the second cavity; a filter membrane is arranged between the first chamber and the second chamber, and the area of the filter membrane is larger than that of the first chamber and that of the second chamber. A first waste liquid hole channel and a first liquid injection hole channel are formed in the upper-layer chip, and the first liquid injection hole channel is communicated with the first cavity; a second waste liquid hole channel is formed in the lower-layer chip, and the second cavity is communicated with the second waste liquid hole channel; the first waste liquid channel is communicated with the second waste liquid channel. The glass slide is provided with a nucleic acid aptamer, and the nucleic acid aptamer is used for enriching exosomes. Through the arrangement, the exosome enrichment in serum or cell culture supernatant is realized, and the exosome enrichment liquid is obtained.

The embodiment of the invention relates to the technical field of super-resolution fluorescence microscopes, in particular to an optical super-lens light sheet endoscopic imaging system and method. Phase control is performed on short-wavelength laser through the light sheet super lens, so that incident light incident on a sample is finely adjusted to be in a light sheet form, the short-wavelength laser after phase control is irradiated on the sample, the light sheet and the sample interact to excite fluorescence, and meanwhile, phase control is performed on long-wavelength laser through the OAM light super lens. The obtained OAM light irradiates a sample, the OAM light quenches a fluorescent substance at a specified position in a fluorescent light spot excited by the short-wavelength laser, so that the diffraction area of the fluorescent light spot is reduced, and finally, the fluorescent light reserved after the OAM light irradiation of the sample is converged to a photodiode through a converging super lens for fluorescence power detection. On the basis of scanning samples such as living cells, the imaging resolution is improved.

According to some embodiments of the present disclosure, a medical image analysis method performed by a computing device is disclosed. The method may include: acquiring a first dataset including a medical image obtained by photographing the prosthesis, inserted into the body, by a first photographing device and a second dataset including a medical image obtained by photographing the prosthesis, inserted into the body, by a second photographing device, wherein the first dataset includes a medical image having a higher resolution than the second dataset; training an analysis model for determining cell type information of the prosthesis by using the first dataset; and validating the analysis model by using the first dataset and the second dataset.

The utility model belongs to cell preparation technical field, concretely relates to a kind of tissue disrupter for cell preparation, including cell disrupter body, amplitude rod is installed in cell disrupter body inner wall, cell disrupter body inner wall installs lifting platform, lifting platform transverse end surface abuts beaker, silica gel pad that beaker surface abuts limit ring inner wall is bonded, limit ring other side surface is connected with moving rod, moving rod surface is slidably connected in the sliding slot of the top of mounting box, mounting box inner wall surface is connected with limit rod, limit rod other side surface is connected with fixed plate, the utility model, by the mutual cooperation between limit ring, moving rod, mounting box, sliding slot, limit rod, fixed plate, dovetail block, spring and side block, the beaker placed on lifting platform is clamped and limited, to avoid its in the process of being broken by ultrasonic vibration driven displacement, cause the influence cell breakage processing uniformity and security.