A cell preparation preparation with a tissue disrupter
By using a combination of structures such as limiting rings to clamp the beaker in the tissue homogenizer, the problem of beaker displacement caused by ultrasonic vibration was solved, achieving uniformity and safety in cell disruption and avoiding sample loss.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- ZHONGZHEN (SHENZHEN) BIOMEDICAL RES CO LTD
- Filing Date
- 2025-07-31
- Publication Date
- 2026-07-07
AI Technical Summary
In existing ultrasonic cell disruption operations, the ultrasonic transducer converts electrical energy into mechanical energy, generating high-frequency vibrations. The amplitude transformer further amplifies these vibrations and transmits them to the sample, causing the beaker to vibrate and change position, affecting the uniformity of cell disruption and potentially leading to the beaker tipping over and sample loss.
A tissue disruptor for cell preparation was designed. Through a combination of a limiting ring, a moving rod, a mounting box, a slide, a limiting rod, a fixing plate, a dovetail block, a spring, and a side block, the beaker is clamped and limited to prevent it from shifting due to ultrasonic vibration during the disruption process, thus ensuring the uniformity and safety of the disruption.
This effectively prevents the beaker from shifting during ultrasonic vibration, ensuring the uniformity and safety of cell disruption, preventing sample spillage, and reducing losses.
Smart Images
Figure CN224467808U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of cell preparation technology, specifically relating to a tissue disruptor for cell preparation. Background Technology
[0002] In the field of modern biomedicine, cell therapy, as a highly promising treatment approach, is bringing new hope to the fight against many intractable diseases. From precision immunotherapy for cancer to cell repair of neurodegenerative diseases, and cutting-edge exploration of organ regeneration, cell therapy, with its unique therapeutic mechanisms and significant efficacy, is gradually becoming a focus of medical research and clinical application. Cellular preparations, as the core material basis of cell therapy, are directly affected by the quality and preparation process of these preparations, which in turn determine the success or failure of cell therapy.
[0003] One of the key starting steps in the preparation of cell preparations is tissue disruption. The purpose of tissue disruption is to efficiently and gently break down biological tissues, such as animal liver and kidneys, or diseased or donated tissues from humans, into single-cell suspensions. This allows for subsequent processes such as cell separation, culture, expansion, and modification, ultimately leading to the preparation of cell preparations that meet therapeutic needs.
[0004] When processing cell preparations using an ultrasonic cell disruptor, the container containing the cell preparation is first placed in the sample processing chamber of the instrument, and the ultrasonic amplitude transformer is immersed to a suitable depth in the preparation. After the instrument is turned on, the power supply provides power to the ultrasonic transducer. The transducer converts electrical energy into mechanical vibration energy at ultrasonic frequencies through the inverse piezoelectric effect, which is then amplified by the amplitude transformer and transmitted to the cell preparation, inducing cavitation. Cavitation bubbles are formed in the negative pressure phase of the ultrasonic waves, and the cavitation bubbles close rapidly in the positive pressure phase, generating local high temperature, high pressure, shock waves, and microjets, which destroy the cell membrane, release the cell contents, and also destroy intracellular organelles. During processing, the disruption effect can be controlled by adjusting parameters such as ultrasonic power, time, and pulse mode through the instrument control system. In order to avoid excessive temperature affecting the activity of biomolecules, some instruments are also equipped with a cooling system.
[0005] In existing ultrasonic cell disruption operations, the ultrasonic transducer converts electrical energy into mechanical energy, generating high-frequency vibrations. The amplitude transformer further amplifies these vibrations and transmits them to the sample. This intense vibration is transmitted through the liquid medium to the beaker wall, causing the beaker to experience a significant vibrational force and move on the stage. After the beaker shifts, the relative position of the ultrasonic amplitude transformer and the cell preparation inside the beaker changes, resulting in changes in the propagation path and energy distribution of the ultrasonic waves within the cell preparation. This leads to inconsistent cell disruption. In severe cases of beaker displacement, the beaker may tip over, causing the cell preparation to spill out and resulting in sample loss. Utility Model Content
[0006] The purpose of this invention is to provide a tissue disruptor for cell preparation, aiming to solve the problem in existing ultrasonic disruption processes where the ultrasonic transducer converts electrical energy into mechanical energy, generating high-frequency vibrations. The amplitude transformer further amplifies these vibrations and transmits them to the sample. This intense vibration is transmitted to the beaker wall through the liquid medium, causing the beaker to experience a large vibrational force and move on the stage. After the beaker shifts, the relative position of the ultrasonic amplitude transformer and the cell preparation inside the beaker changes, resulting in changes in the propagation path and energy distribution of the ultrasonic waves in the cell preparation. This leads to inconsistent cell disruption, and in severe cases, the beaker may tip over, causing the cell preparation to spill out and resulting in sample loss.
[0007] To achieve the above objectives, this utility model provides the following technical solution: a tissue disruptor for cell preparation, comprising a cell disruptor body, an amplitude transformer rod installed on the inner wall of the cell disruptor body, a lifting platform installed on the inner wall of the cell disruptor body, a beaker abutting the horizontal end surface of the lifting platform, a silicone pad adhered to the inner wall of the beaker, a moving rod connected to the other side surface of the limiting ring, the moving rod slidably connected to a groove opened at the top of the mounting box, a limiting rod connected to the inner wall surface of the mounting box, a bottom lifting platform connected to the top surface of the mounting box, a fixing plate connected to the other side surface of the limiting rod, a dovetail groove opened on the surface of the fixing plate to engage with a dovetail block, a spring connected to the other side surface of the dovetail block, the dovetail block engaging with the dovetail groove surface opened on the side block surface, an insert block engaging with the front end surface of the mounting box, and an insert block connected to the other side surface of the insert block to the box cover surface.
[0008] As a preferred embodiment of the tissue disruptor for preparing cell preparations according to this utility model, the limiting ring is a semi-circular structure, and the shape and size of its inner wall are adapted to the beaker.
[0009] As a preferred embodiment of the tissue disruptor for preparing cell preparations according to this utility model, the moving rod has an "L" shaped structure, the shape and size of its longitudinal end are adapted to the sliding groove, and the two sets of sliding grooves are symmetrically opened on both sides of the top of the mounting box.
[0010] In a preferred embodiment of the tissue disruptor for preparing cell preparations according to this invention, the fixing plate, dovetail block, spring, and side block form an elastic connection structure.
[0011] As a preferred embodiment of the tissue disruptor for preparing cell preparations according to this utility model, the surface of the fixing plate and the side block is provided with dovetail grooves, the shape and size of which are adapted to the dovetail blocks, and the dovetail blocks are symmetrically distributed on both sides of the spring.
[0012] As a preferred embodiment of the tissue disruptor for preparing cell preparations according to this utility model, the front end of the mounting box has symmetrical trapezoidal grooves, the shape and size of which are adapted to the insert block.
[0013] Compared with the prior art, the beneficial effects of this utility model are:
[0014] The beaker placed on the lifting platform is clamped and limited by the cooperation between the limiting ring, moving rod, mounting box, slide, limiting rod, fixing plate, dovetail block, spring and side block, so as to avoid the displacement caused by ultrasonic vibration during the crushing process, which would affect the uniformity and safety of cell crushing processing.
[0015] Meanwhile, by utilizing the cooperation between the fixing plate, dovetail block, spring, side block, insert block and lid, the aging spring can be disassembled and replaced, thereby ensuring the spring's contraction force and the stability of the beaker's clamping. Attached Figure Description
[0016] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:
[0017] Figure 1 This is a schematic diagram of the main structure of this utility model;
[0018] Figure 2 This is a side view sectional structural diagram of the present invention;
[0019] Figure 3 This is an exploded structural diagram of the limiting ring and mounting box of this utility model;
[0020] Figure 4 This utility model Figure 3 Enlarged structural diagram of section A.
[0021] In the diagram: 1. Cell disruptor body; 2. Amplitude bar; 3. Lifting platform; 4. Beaker; 5. Limiting ring; 6. Moving rod; 7. Mounting box; 8. Slide groove; 9. Limiting rod; 10. Fixing plate; 11. Dovetail block; 12. Spring; 13. Side block; 14. Insert block; 15. Box cover. Detailed Implementation
[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0023] Please see Figures 1-4This utility model provides the following technical solution: a tissue disruptor for preparing cell preparations, comprising a cell disruptor body 1, an amplitude transformer 2 installed on the inner wall of the cell disruptor body 1, a lifting platform 3 installed on the inner wall of the cell disruptor body 1, a beaker 4 abutting the transverse end surface of the lifting platform 3, a silicone pad adhered to the inner wall of a limiting ring 5 abutting the surface of the beaker 4, a moving rod 6 connected to the other side surface of the limiting ring 5, a sliding groove 8 slidably connected to the surface of the moving rod 6 in the top of a mounting box 7, a limiting rod 9 connected to the inner wall surface of the mounting box 7, a bottom of the lifting platform 3 connected to the top surface of the mounting box 7, and a fixing plate 10 connected to the other side surface of the limiting rod 9. A dovetail groove is opened on the surface of the dovetail block 11 to engage with the dovetail block 11. A spring 12 is connected to the other side surface of the dovetail block 11. The surface of the dovetail block 11 engages with the dovetail groove on the surface of the side block 13. The front surface of the mounting box 7 engages with the insert block 14. The other side surface of the insert block 14 is connected to the surface of the box cover 15. In this design, the cell disruptor body 1, the amplitude rod 2, and the lifting platform 3 constitute the main body of the ultrasonic cell disruptor. A large number of related components are set in the main body of the ultrasonic cell disruptor. Since they are existing technologies and the core content of this technical solution is irrelevant to them, they will not be described in detail in this technical solution.
[0024] The main model of the ultrasonic cell disruptor in this solution is: LJ-CP150.
[0025] In use: The main body of the ultrasonic cell disruptor utilizes the cavitation effect of ultrasound. The ultrasonic generator of the instrument converts the mains power into 20-25KHz alternating power, which is supplied to the transducer. The barium zirconate titanate piezoelectric vibrator in the transducer undergoes elastic deformation with the alternating voltage, causing the transducer to vibrate longitudinally. The vibration is transmitted to the cell preparation placed in the beaker 4 through the titanium alloy amplitude transformer 2. In the liquid, the ultrasound generates positive and negative pressure changes, forming microbubbles. The bubbles close rapidly in the positive pressure phase, generating strong shear force, shock wave and instantaneous high temperature and high pressure, thereby destroying the cell wall and cell membrane, releasing the cell contents, and achieving the purpose of cell disruption.
[0026] Preferably, the limiting ring 5 has a semi-circular structure, and its inner wall shape and size are adapted to the beaker 4.
[0027] In practical use, the limiting ring 5 connected to the surface of the moving rod 6 can abut against the surface of the beaker 4. The inner wall surface of the limiting ring 5 is bonded with a silicone pad, which increases the friction at the intersection of the beaker 4 and the limiting ring 5, so that the beaker 4 can be stably clamped. At the same time, the silicone pad on the inner wall of the limiting ring 5 creates a flexible buffer at the intersection of the beaker 4 and the limiting ring 5, avoiding squeezing damage to the surface of the beaker 4.
[0028] Preferably, the movable rod 6 has an "L" shaped structure, and its longitudinal end shape and size are adapted to the slide groove 8, and two sets of slide grooves 8 are symmetrically opened on both sides of the top of the mounting box 7.
[0029] In practical use, the movable rod 6, which is slidably connected to the slide groove 8, is pulled to both sides, and the movement of the movable rod 6 is guided and limited by the two sets of slide grooves 8 opened on the mounting box 7.
[0030] Preferably, the fixed plate 10, the dovetail block 11, the spring 12 and the side block 13 form an elastic connection structure.
[0031] In practical use, the fixed plate 10 and side block 13, which are elastically connected to both sides of the spring 12, can elastically adjust the distance between the moving rods 6 on both sides.
[0032] Preferably, dovetail grooves are formed on the surfaces of the fixing plate 10 and the side block 13, and their shape and size are adapted to the dovetail block 11. The dovetail blocks 11 are symmetrically distributed on both sides of the spring 12.
[0033] In actual use, pull upwards to engage the dovetail block 11 that is inserted into the dovetail groove on the fixed plate 10 and the side block 13, thereby replacing the spring 12.
[0034] Preferably, trapezoidal grooves are symmetrically provided at the front end of the mounting box 7, and their shape and size are adapted to the insert block 14.
[0035] In actual use, pull the handle on the surface of the cover 15 outwards, so that the rubber insert 14 disengages from the groove at the front end of the mounting box 7, making it convenient to replace the spring 12 inside the mounting box 7.
[0036] Working principle: When preparing cell preparations, the raw materials to be crushed are placed in the matching beaker 4. Then, the sliding rods 6 connected to the slide grooves 8 are pulled to both sides, allowing the beaker 4 to be placed on the placement grooves on the surface of the lifting platform 3. The circular grooves on the lifting platform 3 can position the beaker 4, placing it below the amplitude rod 2. Then, the sliding rods 6 pulled to both sides are released, causing the springs 12 connecting the sliding rods 6 and the fixed plate 10 to rebound, driving the sliding rods 6 on both sides to slide towards the midpoint on the limit rods 9. The limiting ring 5 connected to the surface of the moving rod 6 can abut against the surface of the beaker 4. The inner wall surface of the limiting ring 5 is bonded with a silicone pad, which increases the friction at the intersection of the beaker 4 and the limiting ring 5, so that the beaker 4 can be stably clamped. At the same time, the silicone pad on the inner wall of the limiting ring 5 creates a flexible buffer at the intersection of the beaker 4 and the limiting ring 5, avoiding squeezing damage to the surface of the beaker 4. This limits the position of the beaker 4 placed on the lifting platform 3, and prevents it from being displaced on the lifting platform 3 due to ultrasonic vibration when the amplitude rod 2 vibrates and breaks the raw material in the beaker 4.
[0037] When it is necessary to change the model of beaker 4, a suitable spring 12 can be selected according to the model of beaker 4. The spring 12 with an appropriate stiffness coefficient (k value) should be matched according to the diameter of beaker 4 to ensure that the elastic force is stable at 5-20N within the normal compression range. This ensures that beaker 4 can be fixed without being squeezed and broken. When it is necessary to replace the spring 12 in the mounting box 7, pull the handle on the surface of the box cover 15 outward to disengage the rubber insert 14 from the groove at the front end of the mounting box 7, thereby exposing the two sets of springs 12 in the mounting box 7. Then pull upward to engage the dovetail block 11 in the dovetail groove on the fixing plate 10 and the side block 13 to replace the spring 12. After replacement, repeat the above operation to engage and fix the box cover 15, thereby covering the spring 12 in the mounting box 7.
[0038] Finally, it should be noted that the above are merely preferred embodiments of this utility model and are not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.
Claims
1. A tissue disruptor for preparing cell preparations, comprising a cell disruptor body (1), characterized in that: An amplitude transformer (2) is installed on the inner wall of the cell disruptor body (1). A lifting platform (3) is installed on the inner wall of the cell disruptor body (1). The horizontal end surface of the lifting platform (3) abuts against a beaker (4). The surface of the beaker (4) abuts against a silicone pad adhered to the inner wall of a limiting ring (5). A moving rod (6) is connected to the other side surface of the limiting ring (5). The surface of the moving rod (6) is slidably connected to a groove (8) opened at the top of the mounting box (7). A limiting rod (9) is connected to the inner wall surface of the mounting box (7). (7) The top surface is connected to the bottom of the lifting platform (3), the other side surface of the limiting rod (9) is connected to the fixing plate (10), the surface of the fixing plate (10) is provided with a dovetail groove to engage with the dovetail block (11), the other side surface of the dovetail block (11) is connected to the spring (12), the surface of the dovetail block (11) is engaged with the dovetail groove surface provided on the side block (13), the front surface of the mounting box (7) is engaged with the insert block (14), and the other side surface of the insert block (14) is connected to the surface of the box cover (15).
2. The tissue disruptor for preparing cell preparations according to claim 1, characterized in that: The limiting ring (5) is a semi-circular structure, and its inner wall shape and size are adapted to the beaker (4).
3. The tissue disruptor for preparing cell preparations according to claim 1, characterized in that: The movable rod (6) has an "L" shaped structure, and its longitudinal end shape and size are adapted to the slide groove (8). The two sets of slide grooves (8) are symmetrically opened on both sides of the top of the mounting box (7).
4. The tissue disruptor for preparing cell preparations according to claim 1, characterized in that: The fixed plate (10), dovetail block (11), spring (12) and side block (13) form an elastic connection structure.
5. The tissue disruptor for preparing cell preparations according to claim 4, characterized in that: The fixed plate (10) and the side block (13) have dovetail grooves on their surfaces, and their shape and size are adapted to the dovetail block (11). The dovetail blocks (11) are symmetrically distributed on both sides of the spring (12).
6. The tissue disruptor for preparing cell preparations according to claim 1, characterized in that: The mounting box (7) has symmetrical trapezoidal grooves at its front end, the shape and size of which are adapted to the insert block (14).