18 results about "Gland cell" patented technology

The invention discloses a protein complex for protecting mitochondria from being damaged, a pharmaceutical composition and application. According to the application, the influence of nitrate on mitochondrial injury in in-vivo radiation-induced salivary gland injury and in-vitro human salivary gland cell injury is evaluated, the action mechanism in the mitochondrial injury is further deeply explored, and a result shows that sialic acid transporter interacts with FKBP8, so that the mitochondrial injury can be inhibited, and the mitochondrial injury can be inhibited. In turn, particles are formed and anchored on the outer mitochondrial membrane, thereby maintaining membrane integrity. Therefore, the invention provides a brand new therapeutic target for preventing radiation-induced salivary gland injury and other mitochondrial membrane related diseases.

The utility model relates to breast nursing technical field especially a breast nursing dredging device, including handle, the surface screw thread connection of handle has the cover cup, the inner chamber of cover cup is provided with the massage head, the inner chamber of cover cup is provided with the adjusting mechanism, the adjusting mechanism includes the massage block, the massage block swing setting is in the inner chamber of cover cup, the surface of massage block is connected with the cannula, the surface of handle is provided with the liquid adding pipe, one end of liquid adding pipe is connected with the connecting pipe, the utility model discloses through the cooperation of massage head, massage block, cannula, liquid adding pipe, connecting pipe, ring pipe, liquid warehouse pipe, driving motor, sliding mouth and ball, is equipped with rolling massage head and sliding mouth, can add the liquid of alleviating automatically in the massage process, utilizes driving motor to drive the ball rolling massage and liquid alleviating combination, can more deeply stimulate breast cell, promotes blood and lymphatic fluid circulation, accelerates the dredging and recovery of breast.

The invention belongs to the technical field of experimental animal models, biomedical research and immunology, and discloses a photocoagulation thymus ischemia animal model, a method and application of the photocoagulation thymus ischemia animal model in rapid simulation of thymus degeneration and immune senescence, and the model shows at least one or more characteristics 1-7 days after induction: thymus atrophy, thymus weight or thymus index is significantly reduced; the cortical-medullary structure of thymus is disordered or disappears; the expression distribution of the Keratin 5 / Keratin 8 is abnormal; thymic cell apoptosis is increased, and lipid deposition is enhanced; the proportion of CD4 + CD8 + double positive T cells is reduced, and the proportion of DN2 / DN3 stage T cells is increased; the initial T cell output is reduced; and the immune function is reduced. The pathological mechanism is clear; the modeling period is short, and the immune senescence phenotype can be stably obtained within several days; the injury focus is controllable, and systemic stress or hormone interference is avoided; the model has high repeatability and is suitable for mechanism research and drug screening.

The present disclosure describes a method for preventing or treating an abnormal mammary gland cytopathy characterized by cell proliferation in a subject. The method comprises administering to the subject an effective amount of an androgen agent in combination with an effective amount of an aromatase inhibitor, increasing the concentration of the androgen agent in serum to a level to increase the intramammary concentration of androgen in the mammary gland resulting from the action of 5 alpha-reductase on the androgen agent, the increased intramammary concentration of the androgen induces inhibition of c-Myc and / or mTOR expression or activity in the mammary gland associated with a lower intramammary concentration of the androgen. The increase in the serum concentration of the androgen agent and the increase in the intramammary concentration of the androgen are maintained for a predetermined period of time without substantially increasing the concentration of the androgen in the serum of the subject. Furthermore, the use of the aforementioned androgen and aromatase inhibitors for reducing the risk of breast cancer progression to hormone-resistant breast cancer, reducing the risk of HER2-positive breast cancer becoming insensitive to targeted HER-2 anti-cancer drug therapy, and for inducing inhibition of c-Myc and / or mTOR expression and / or activity is also described. In embodiments, the androgen agent may be testosterone and androgen 5 [alpha]-dihydrotestosterone (DHT).

The application discloses a preparation method of a 3D model of a maternal-fetal interface constructed by an endometrial organoid in vitro, and the method comprises the following steps: establishing a primary endometrial epithelial organoid and an endometrial stromal cell line in vitro; improving an extracellular matrix for assembling endometrial gland-like organoids and stromal cells; the constructed endometrial organoid model comprises gland cells and stromal cells; the endometrial organoid model is cultured by using a gas-liquid interface, so that the endometrial organoid model has a cavity epithelium and a gland structure; the endometrial organoid model simulates a menstrual cycle of the endometrium and performs function identification. The endometrial organoid model has the cavity epithelium and the gland epithelium for the first time, the tissue structure, the cell composition, the hormone-induced change, the gene expression characteristics of the organoid are similar to those in the body, and the organoid can reproduce the receptivity genes and the dynamicity of cilia which are necessary for embryo implantation in the body; the organoid can be used as a model for studying embryo implantation regulation, endometrial diseases and regeneration mechanisms.

This invention relates to the field of pharmaceutical biotechnology, providing the application of aminoquinoline compounds (such as hydroxychloroquine and chloroquine) in the preparation of drugs for treating hyperparathyroidism. These compounds restore the expression of calcium-sensitive receptors (CaSRs) on the cell membrane surface by inhibiting the lysosomal degradation pathway of CaSRs in parathyroid cells. Experiments have confirmed that 4-aminoquinoline compounds, used alone or in combination with calcimimetics (such as cinacalcet), significantly reduce serum PTH and calcium levels in a model of refractory secondary hyperparathyroidism (SHPT) and inhibit glandular hyperplasia. In particular, the combination therapy regimen exhibits a significant synergistic effect, effectively reversing calcimimetics resistance. This invention is the first to discover a novel use for the antimalarial / antirheumatic drug hydroxychloroquine in the treatment of hyperparathyroidism, realizing a new use for an existing drug; and providing a safe and effective new drug treatment strategy for clinically refractory SHPT.

The invention discloses a thermophilic thermophilic bacteria fermentation product, a preparation method and application thereof and an oil control product, and belongs to the technical field of cosmetics. Thermophilic thermophilic bacteria are cultured in a first-stage seed solution culture medium and a second-stage seed solution culture medium in sequence, the thermophilic thermophilic bacteria are promoted to the logarithmic phase, then a mulberry leaf extracting solution is added for fermentation, and the oil control product is obtained. According to the method, the growth stability of thalli is effectively prolonged, the stability of a fermentation microenvironment is maintained, the metabolic activity of the thermophilic thermophilic bacteria is improved, normal replication of thallus DNA at high temperature can be guaranteed, and the thermophilic thermophilic bacteria can grow at a high speed in the whole growth period. According to the method disclosed by the invention, the accumulation of byproducts can be avoided, active ingredients with an oil control effect in the thermophilic thermophilic bacteria are effectively enriched, and a thermophilic thermophilic bacteria fermentation product which has the effects of improving the mRNA expression quantity of AMPK, effectively inhibiting the activity of 5 alpha-reductase, inhibiting sebaceous gland cells from synthesizing lipid droplets and reducing hormone-induced lipid synthesis is obtained; the oil control activity is excellent.

The invention relates to application of Antarctic red in a skin conditioning agent for synergistically controlling oil and resisting wrinkles, and belongs to the technical field of biology, the Antarctic red has no toxicity to cells when the concentration is not higher than 250 g / mL, gene expression of I-type 5 alpha-reductase in sebaceous gland cells is inhibited in a targeted mode, and excessive synthesis of grease is reduced from the source; the gene expression level of I type and III type collagen in dermal fibroblasts is synchronously improved, so that the internal support structure of the skin is reconstructed, and the anti-wrinkle effect is realized. According to the Antarctic red disclosed by the invention, through the synergistic effect of'descending and ascending ', the Antarctic red is proved to be capable of simultaneously and accurately intervening an'oil-wrinkle' associated pathway, and the limitation of a single functional component is broken through.

The application relates to the technical field of cell culture, and particularly discloses a culture medium for musk deer musk gland cells and a culture method for musk deer musk gland cells. The culture medium for the musk deer musk gland cells comprises a DMEM / F12 culture medium and the following components: 80-120 U / mL penicillin, 0.08-0.12 mg / mL streptomycin, 8-12% fetal bovine serum, 40-60 ng / mL epidermal growth factor, 80-120 ng / mL fibroblast growth factor, 0.06-0.15 ug / mL hydrocortisone, 0.4-0.6 uM / mL TGF-beta inhibitor, 1.6-2.4 mM / mL glutamine and 3.5-6 mM / mL acetyl-L-cysteine. The application further provides a culture method for the musk deer musk gland cells, which comprises the following steps: digestion, cell preparation, primary culture and subculture. The culture medium for the musk deer musk gland cells is used to culture the musk deer musk gland cells, the subculture effect of the musk deer musk gland cells is good, the proliferation capacity of the musk deer musk gland cells is strong, the morphological change of the musk deer musk gland cells is small, and the musk deer musk gland cells can be well kept dry during the subculture.

The invention relates to the technical field of cell engineering and biopharmacy, and discloses a method for promoting synthesis of steroids in forest musk gland cells, which comprises the following steps: determining the tolerance range of cholesterol concentration through a pre-experiment, setting an experimental group, culturing cells, collecting and treating a sample, and detecting by LC-MS (Liquid Chromatography-Mass Spectrometry). According to the method for promoting synthesis of the steroids in the lindel gland cells, the synthesis amount of the seven steroids in the lindel gland cells can be up-regulated by regulating the cholesterol concentration of the culture medium to 10-20 mg / L, the content of pregnenolone is up-regulated by 4.12 times (Plt, 0.05), the content of progesterone is up-regulated by 1.46 times (Plt, 0.05), and the method is remarkably superior to that of existing culture without cholesterol regulation; a complete standardized process from pre-experiment, culture, sample treatment to detection is provided, the experiment result repeatability is high, and the method can be directly applied to industrial production.

The application discloses a method for culturing sweat gland cells to obtain a sweat gland organoid and application thereof, and belongs to the technical field of bioengineering, wherein the sweat gland cells can be cultured by using a three-dimensional culture medium provided by the application, and a sweat gland organoid with high cell activity and bidirectional differentiation potential is obtained; after being transplanted into a full-thickness injury mouse on the sole and back, the sweat gland organoid not only can promote the regeneration and function recovery of the sweat gland, but also can promote the healing of the skin wound, and can be used for preparing a cell product for repairing skin injury and recovering the function of an accessory organ, and has a wide application prospect.

This application relates to the technical field of cell culture, specifically disclosing a culture medium for forest musk gland cells, its preparation method, and its application. The culture medium disclosed in this application uses DMEM / F12 as the basal medium and contains the following components as functional additives at various concentrations: penicillin 80-120 U / ml, streptomycin 0.08-0.12 mg / ml, FBS 8-12 v / v%, non-essential amino acids 0.7-1.3 v / v%, growth factors 20-40 ng / ml, and vitamins 0.05-0.2 μg / ml. This application also discloses the preparation method of the above-mentioned culture medium and its application in the culture of forest musk gland cells. When forest musk gland cells are cultured using the culture medium disclosed in this application, the cell proliferation rate is faster, the cell activity is stronger, and the cell adhesion rate is higher.

The invention discloses an induced differentiation method of sebaceous gland cells, which comprises the following specific steps: adding epidermal stem cells into an induction culture medium, and carrying out induced culture for 5-7 days to obtain the sebaceous gland cells, wherein the adopted induction culture medium is not added with insulin and specifically comprises the following components: 50% of a keratinocyte culture medium, 50% of a sebaceous gland cell culture medium, 1% of antibiotics, 20-40 ng / mL of EGF, 10-40 ng / mL of KGF and 5-20 ng / mL of rosiglitazone. Growth factors EGF and KGF and rosiglitazone are simultaneously added into the induction culture medium, insulin is completely not added, normal division and proliferation of cells are guaranteed, and sebaceous gland cells which are good in growth state and high in differentiation degree are obtained in a short culture period; compared with SZ95, the expression quantities of various sebaceous gland cell markers are similar.

The application provides a thyroid cell pathological image information region classification model construction method and device. In the preparation of training data, the image blocks segmented from the panoramic pathological image are divided into a primary data set and an advanced data set, and the pathological experts add annotation labels to each image block in the advanced data set. In the training stage, the primary data set is used to preliminarily train a first-level network to train the model to recognize the cell features in the image block, then the parameters of the first-level network after training are used as the initial parameters of a second-level network, and the second-level network is fine-tuned using the advanced data set to obtain a thyroid cell pathological image information region classification model for identifying useful information regions. In this way, the annotation labels based on the pathological experts help to deepen the understanding of the neural network for the useful information region, and secondly, the two-level training fine-tuning scheme can comprehensively learn the objective cell features and the clinical prior knowledge of the pathological experts, effectively improving the performance of the model.

A problem addressed by the present invention is to provide a method for producing pancreatic β cells from endothermal cells that have been induced to differentiate from pluripotent stem cells, wherein the method allows the production of pancreatic β cells of high quality, and to provide pancreatic progenitor (PP) cells, pancreatic endocrine precursor (EP) cells, and pancreatic β cells produced by the above-mentioned method. The present invention provides a method for producing pancreatic β cells that includes culturing primitive gut tube (PGT) cells, which have been induced to differentiate from pluripotent stem cells, in the presence of a protein kinase C (PKC) activator, thereby producing posterior foregut (PFG) cells; next culturing the cells in the presence of retinoic acid or an analog thereof, thereby producing pancreatic progenitor (PP) cells; next culturing the cells in the presence of a Notch signaling inhibitor and a ROCK signaling inhibitor, thereby producing pancreatic endocrine precursor (EP) cells; and next culturing the cells in the presence of an insulin receptor signaling activator, transferrin, and selenous acid, thereby producing pancreatic β cells.

The application discloses a kind of compositions for regulating oil secretion and preparation method and application thereof;The composition includes the following mass percentage of ingredients: azelate di-glycine potassium 1-5%;APN activity inhibitor 1-5%;Plankton extract 1-4%;Nicotinamide 1-2%;The composition of the application inhibits the activity of type I 5alpha-reductase, cyclooxygenase-2 and aminopeptidase N in sebaceous gland cells, improves keratinization of hair follicle orifice, dredges pores, and regulates water and oil balance, and inhibits sebaceous gland cell activity through multi-pathway synergy, jointly regulates the secretion of oil;It fundamentally inhibits the synthesis and secretion of oil in sebaceous gland cells.The composition of the application is suitable for application in various cosmetics for regulating the secretion of oil on scalp or skin, and has good industrial application prospect.

The invention belongs to the technical field of biosensor detection, and discloses a preparation method of an LIG-based all-solid-state ion sensor array for cell multi-ion real-time monitoring. The LIG-based all-solid-state ion sensor array is integrally formed through a laser direct writing technology, PEDOT: PSS is electrochemically deposited between an LIG substrate and an ion selective membrane to serve as a solid contact layer, formation of an interface water layer is inhibited, long-term potential drifts of Na < + >, K < + > and Ca < 2 + > electrodes are remarkably reduced, and the stability is improved by more than ten times. The constructed sensor has high sensitivity, good reversibility and low detection limit on Na < + >, K < + >, Ca < 2 + > and H < + >. The array can continuously monitor the dynamic change of the extracellular ion concentration under the stimulation of fMLP in real time, and clearly capture more severe ion response signals of breast cancer cells under chemical stimulation than normal breast cells.

The invention relates to the technical field of animal nutrition science, and discloses a method for synthesizing casein of primary bovine mammary gland cells (BMECs) by regulating leucine through a DDIT3 gene, which comprises the following steps: S1, preparation and pretreatment of primary BMECs; s2, the pretreated primary BMECs are subjected to grouped culture, a control group, an essential amino acid (EAA) lack model group and a leucine treatment group are set, and a culture medium which does not contain all EAA and is supplemented with leucine is adopted in the leucine treatment group for culture; s3, regulating and controlling DDIT3 gene expression and combining a signal channel formed by upstream and downstream genes to realize regulation and control on BMECs casein synthesis. By supplementing leucine with a specific concentration in an EAA lacking environment and combining pathway regulation and control, beta-casein synthesis of BMECs is accurately activated, the daily ration nitrogen utilization efficiency is effectively improved, and the breeding cost and the environmental pressure are reduced.