40 results about "Gland cell" patented technology

The application relates to a kind of gland cell image segmentation methods based on selective multi-branch hollow convolution, input gland cell image dataset, with improved UNet as main network, construct gland cell segmentation network based on selective multi-branch hollow convolution, the network is captured more advanced features with context encoding network, with channel attention module adaptive recalibration channel feature response and highlight the most relevant feature channel, with multi-scale selection module amplifies effective information and suppresses redundant information, selects the appropriate scale of hollow convolution branch;With the input gland cell image dataset, the segmentation network is trained to obtain a stable segmentation network;Input to be segmented gland cell image, with stable segmentation network to carry out gland cell segmentation.The application has better generalization ability, and is especially suitable for the size, shape change difference of gland in colorectal polyp.

The application discloses application of a reagent for detecting LRRN3 expression level in preparation of a benign prostatic hyperplasia diagnosis product. It is proved through cell function experiments and animal experiments that overexpression of the LRRN3 gene can inhibit the proliferation of prostate cells, block the cell cycle and promote cell apoptosis, thereby effectively inhibiting the prostatic hyperplasia. The discovery provides a new target and treatment strategy for the treatment of the prostatic hyperplasia, and provides a brand-new thought and method for the treatment of the prostatic hyperplasia. Based on the research results, a gene therapy drug taking the LRRN3 gene as a target can be further developed, for example, a lentivirus vector carrying the LRRN3 gene or other gene delivery systems are constructed and used for the treatment of the prostatic hyperplasia. In addition, the LRRN3 gene can be combined with other treatment methods to improve the treatment effect.

Disclosed herein are compositions and methods related to differentiation of stem cells into pancreatic endocrine cells. In some aspects, the methods provided herein relate to generation of pancreatic β cell, α cell, δ cells, and EC cells in vitro. In some aspects, the disclosure provides pharmaceutical compositions including the cells generated according to the methods disclosed herein, as well as methods of treatment making use thereof.

The invention discloses a protein complex for protecting mitochondria from being damaged, a pharmaceutical composition and application. According to the application, the influence of nitrate on mitochondrial injury in in-vivo radiation-induced salivary gland injury and in-vitro human salivary gland cell injury is evaluated, the action mechanism in the mitochondrial injury is further deeply explored, and a result shows that sialic acid transporter interacts with FKBP8, so that the mitochondrial injury can be inhibited, and the mitochondrial injury can be inhibited. In turn, particles are formed and anchored on the outer mitochondrial membrane, thereby maintaining membrane integrity. Therefore, the invention provides a brand new therapeutic target for preventing radiation-induced salivary gland injury and other mitochondrial membrane related diseases.

The utility model relates to breast nursing technical field especially a breast nursing dredging device, including handle, the surface screw thread connection of handle has the cover cup, the inner chamber of cover cup is provided with the massage head, the inner chamber of cover cup is provided with the adjusting mechanism, the adjusting mechanism includes the massage block, the massage block swing setting is in the inner chamber of cover cup, the surface of massage block is connected with the cannula, the surface of handle is provided with the liquid adding pipe, one end of liquid adding pipe is connected with the connecting pipe, the utility model discloses through the cooperation of massage head, massage block, cannula, liquid adding pipe, connecting pipe, ring pipe, liquid warehouse pipe, driving motor, sliding mouth and ball, is equipped with rolling massage head and sliding mouth, can add the liquid of alleviating automatically in the massage process, utilizes driving motor to drive the ball rolling massage and liquid alleviating combination, can more deeply stimulate breast cell, promotes blood and lymphatic fluid circulation, accelerates the dredging and recovery of breast.

The invention belongs to but not limited to the technical field of biomedicine, and discloses a composition with a cell protection effect and application thereof, the composition comprises five monomers of cycloastragenol, resveratrol, ginsenoside, tetrahydrocurcumin and cellulose, and can also comprise a moisturizing composition and a moisturizing composition. The composition provided by the invention can accurately act on vaginal smooth muscle cells and gland cells, and can be used for recovering or improving endocrine balance by improving cell activity, promoting elastin synthesis and repairing cell injury in multiple ways, improving telomerase activity of a reproductive system and regulating immune response, so that the curative effect of the composition is improved. Various injury factors such as exogenous factors, traction of production machinery and age increase are effectively resisted, and the method has wide application prospects in the fields of postpartum reproductive system rehabilitation, maintenance of normal functions of the reproductive system, prevention and improvement of conditions such as vagina relaxation, dryness and poor injury repair and the like, and prevention and treatment of related diseases of the reproductive system and health care.

The invention belongs to the technical field of experimental animal models, biomedical research and immunology, and discloses a photocoagulation thymus ischemia animal model, a method and application of the photocoagulation thymus ischemia animal model in rapid simulation of thymus degeneration and immune senescence, and the model shows at least one or more characteristics 1-7 days after induction: thymus atrophy, thymus weight or thymus index is significantly reduced; the cortical-medullary structure of thymus is disordered or disappears; the expression distribution of the Keratin 5 / Keratin 8 is abnormal; thymic cell apoptosis is increased, and lipid deposition is enhanced; the proportion of CD4 + CD8 + double positive T cells is reduced, and the proportion of DN2 / DN3 stage T cells is increased; the initial T cell output is reduced; and the immune function is reduced. The pathological mechanism is clear; the modeling period is short, and the immune senescence phenotype can be stably obtained within several days; the injury focus is controllable, and systemic stress or hormone interference is avoided; the model has high repeatability and is suitable for mechanism research and drug screening.

The invention relates to the field of biology, in particular to an in-vitro separation, culture and identification method of human primary sweat gland muscle epithelial cells. The invention aims to solve the problems in the background technology, and provides an in-vitro separation, culture and identification method of human primary sweat gland epithelial cells, which is characterized by comprising the following steps: acquiring a skin specimen, preserving at low temperature, cleaning, digesting, cleaning, digesting, irradiating and disinfecting, adhering an implant to the wall, separating and planting, abandoning a culture medium, and observing and photographing. The specific operation modes are as follows: (1) acquiring a skin specimen: acquiring the skin specimen through an operation; and (2) low-temperature preservation: putting into a sterile PBS (Phosphate Buffer Solution) containing double antibodies of 1 * penicillin (100U / ml)-streptomycin (0.1 g / ml) for low-temperature preservation. The method disclosed by the invention has the beneficial effects that sweat gland cells are cultured by utilizing a sweat gland tissue block adherent method, sweat gland muscle epithelial cells are obtained and separated by using pancreatin digestive juice at a time point when the cells grow for one week, and in addition, the cells are different in adherent capability.

The present disclosure describes a method for preventing or treating an abnormal mammary gland cytopathy characterized by cell proliferation in a subject. The method comprises administering to the subject an effective amount of an androgen agent in combination with an effective amount of an aromatase inhibitor, increasing the concentration of the androgen agent in serum to a level to increase the intramammary concentration of androgen in the mammary gland resulting from the action of 5 alpha-reductase on the androgen agent, the increased intramammary concentration of the androgen induces inhibition of c-Myc and / or mTOR expression or activity in the mammary gland associated with a lower intramammary concentration of the androgen. The increase in the serum concentration of the androgen agent and the increase in the intramammary concentration of the androgen are maintained for a predetermined period of time without substantially increasing the concentration of the androgen in the serum of the subject. Furthermore, the use of the aforementioned androgen and aromatase inhibitors for reducing the risk of breast cancer progression to hormone-resistant breast cancer, reducing the risk of HER2-positive breast cancer becoming insensitive to targeted HER-2 anti-cancer drug therapy, and for inducing inhibition of c-Myc and / or mTOR expression and / or activity is also described. In embodiments, the androgen agent may be testosterone and androgen 5 [alpha]-dihydrotestosterone (DHT).

The invention belongs to the field of medicine preparation, and relates to application of a CPT1A inhibitor in preparation of a sepsis medicine. By constructing a mouse cecum ligation perforation sepsis model, it is found that the number of thymus DP cells is remarkably reduced after 24h of CLP operation, and T cell subpopulation distribution is abnormal. The loss of the DP cells further leads to hindering of output of newborn T cells, and finally the number of T cells in the spleen and peripheral blood is remarkably reduced. A CPT1A inhibitor Etomoxir is used for intervention, and the rescue effect of the CPT1A inhibitor Etomoxir on mitochondrial functions, cell apoptosis and T cell subset distribution is verified. Experimental results show that CPT1A plays an important role in the death process of thymus T cells caused by sepsis and is expected to become a target spot for treating sepsis in the future.

The invention relates to the technical field of medicine preparation, in particular to application of a serine protease inhibitor in preparation of medicine for treating hyperhidrosis, animal experiments prove that the serine protease inhibitor can inhibit sweat gland cell activity, new use of old medicine is achieved, sweat gland secretion is improved, and the effect of treating hyperhidrosis is achieved. And a new scheme is provided for development of drugs for treating the polyhamia.

The application discloses a preparation method of a 3D model of a maternal-fetal interface constructed by an endometrial organoid in vitro, and the method comprises the following steps: establishing a primary endometrial epithelial organoid and an endometrial stromal cell line in vitro; improving an extracellular matrix for assembling endometrial gland-like organoids and stromal cells; the constructed endometrial organoid model comprises gland cells and stromal cells; the endometrial organoid model is cultured by using a gas-liquid interface, so that the endometrial organoid model has a cavity epithelium and a gland structure; the endometrial organoid model simulates a menstrual cycle of the endometrium and performs function identification. The endometrial organoid model has the cavity epithelium and the gland epithelium for the first time, the tissue structure, the cell composition, the hormone-induced change, the gene expression characteristics of the organoid are similar to those in the body, and the organoid can reproduce the receptivity genes and the dynamicity of cilia which are necessary for embryo implantation in the body; the organoid can be used as a model for studying embryo implantation regulation, endometrial diseases and regeneration mechanisms.

This invention relates to the field of pharmaceutical biotechnology, providing the application of aminoquinoline compounds (such as hydroxychloroquine and chloroquine) in the preparation of drugs for treating hyperparathyroidism. These compounds restore the expression of calcium-sensitive receptors (CaSRs) on the cell membrane surface by inhibiting the lysosomal degradation pathway of CaSRs in parathyroid cells. Experiments have confirmed that 4-aminoquinoline compounds, used alone or in combination with calcimimetics (such as cinacalcet), significantly reduce serum PTH and calcium levels in a model of refractory secondary hyperparathyroidism (SHPT) and inhibit glandular hyperplasia. In particular, the combination therapy regimen exhibits a significant synergistic effect, effectively reversing calcimimetics resistance. This invention is the first to discover a novel use for the antimalarial / antirheumatic drug hydroxychloroquine in the treatment of hyperparathyroidism, realizing a new use for an existing drug; and providing a safe and effective new drug treatment strategy for clinically refractory SHPT.

The invention discloses a thermophilic thermophilic bacteria fermentation product, a preparation method and application thereof and an oil control product, and belongs to the technical field of cosmetics. Thermophilic thermophilic bacteria are cultured in a first-stage seed solution culture medium and a second-stage seed solution culture medium in sequence, the thermophilic thermophilic bacteria are promoted to the logarithmic phase, then a mulberry leaf extracting solution is added for fermentation, and the oil control product is obtained. According to the method, the growth stability of thalli is effectively prolonged, the stability of a fermentation microenvironment is maintained, the metabolic activity of the thermophilic thermophilic bacteria is improved, normal replication of thallus DNA at high temperature can be guaranteed, and the thermophilic thermophilic bacteria can grow at a high speed in the whole growth period. According to the method disclosed by the invention, the accumulation of byproducts can be avoided, active ingredients with an oil control effect in the thermophilic thermophilic bacteria are effectively enriched, and a thermophilic thermophilic bacteria fermentation product which has the effects of improving the mRNA expression quantity of AMPK, effectively inhibiting the activity of 5 alpha-reductase, inhibiting sebaceous gland cells from synthesizing lipid droplets and reducing hormone-induced lipid synthesis is obtained; the oil control activity is excellent.

The invention relates to application of Antarctic red in a skin conditioning agent for synergistically controlling oil and resisting wrinkles, and belongs to the technical field of biology, the Antarctic red has no toxicity to cells when the concentration is not higher than 250 g / mL, gene expression of I-type 5 alpha-reductase in sebaceous gland cells is inhibited in a targeted mode, and excessive synthesis of grease is reduced from the source; the gene expression level of I type and III type collagen in dermal fibroblasts is synchronously improved, so that the internal support structure of the skin is reconstructed, and the anti-wrinkle effect is realized. According to the Antarctic red disclosed by the invention, through the synergistic effect of'descending and ascending ', the Antarctic red is proved to be capable of simultaneously and accurately intervening an'oil-wrinkle' associated pathway, and the limitation of a single functional component is broken through.

Disclosed herein are compositions and methods related to differentiation of stem cells into pancreatic islet cells. In some aspects, the methods provided herein relate to generation of pancreatic β cell, α cell, δ cells, and EC cells in vitro. In some aspects, the disclosure provides pharmaceutical compositions including the cells generated according to the methods disclosed herein, as well as methods of treatment making use thereof.

Embodiments of the present disclosure relate to novel compositions and methods for generating thymic cells. In certain embodiments, thymus cells can be differentiated from pluripotent stem cells (PSC), anterior primitive stripe (APS) cells, definitive endoderm (DE) cells, anterior intestinal endoderm (AFE) cells, pharyngeal endoderm (PE) cells, abdominal pharyngeal endoderm (VPE) cells, and third pharyngeal sac endoderm (TPPE) cells using the compositions and methods disclosed herein. In certain embodiments, thymic cells generated by the compositions, systems, and methods disclosed herein can be used to treat health conditions.

The invention discloses a culture medium and a method for constructing an immune organ-like model and application, belongs to the field of biological medicines, and mainly solves the problem that in-vitro human immune organ model construction and human immunology process simulation are difficult to realize. According to the method, human peripheral blood mononuclear cells (PBMC), thymus cells, tonsil cells, spleen cells or lymph node primary cells are inoculated in a culture plate with a specific structure, and an immune environment with a physiological three-dimensional (3-dimensional) structure is formed by applying a specific culture medium and conditions, so that long-term, efficient and convenient culture of the immune organ is realized; the immune tissue function of a human body is simulated. The technology involved in the invention can be applied to the fields of deep research of accelerated immunology mechanism, drug screening and evaluation, patient selection, disease detection and the like.

The invention belongs to the field of biological medicine, and particularly relates to application of acidic tripeptide to preparation of drugs for resisting viruses and virus diseases of aquatic animals. The acidic tripeptide Tripeptide is a polypeptide which is composed of three amino acids, namely, Leu, Asp and Val. The application has the advantages that firstly, the key effect of the VP7 in virus infection is verified from the positive and negative aspects, the infection of grass carp reovirus (GCRV) can be promoted by transfecting VP7 plasmids to grass carp gonad cell lines (GCO), and the GCRV infection can be inhibited by blocking VP7 antibodies; and (2) in-vivo and in-vitro experiments prove that GCRV virus infection can be effectively inhibited by applying the acidic tripeptide from the VP7 to treat. And (3) the invention discloses a brand new biological function of the virus outer capsid structure protein source Tripetide, and effectively blocks the virus from invading cells, thereby reducing host infection. The tripeptide is non-toxic to grass carp cells in an effective concentration range. The research provides important theoretical and experimental basis for developing efficient and safe drugs for resisting viral diseases of aquatic animals.

The invention discloses application of furochromone in preparation of a medicine for treating triple negative breast cancer. The invention relates to novel application of furochromone in treatment of triple negative breast cancer, research shows that furochromone can inhibit cell proliferation and migration of triple negative breast cancer, can significantly inhibit tumor growth of TNBC, reduce tumor size and weight and relieve symptoms of model animals, has a treatment effect superior to that of positive drugs, and has in-vivo safety. Meanwhile, the furochromone is relatively low in toxicity to normal mammary gland cells, and relatively high in selectivity and safety. Therefore, a foundation is laid for developing more medicines for treating the triple-negative breast cancer, the furochromone becomes a new choice of the medicines for treating the triple-negative breast cancer, and the furochromone has important significance for treating the triple-negative breast cancer.

The invention discloses an oil control composition for regulating sebaceous glands and a preparation method and application thereof, and belongs to the technical field of cosmetics, the oil control composition for regulating sebaceous glands comprises the following components in parts by mass: 0.5-10 parts of 10-hydroxydecanoic acid, 5-25 parts of sarcosine and 5-50 parts of an oil control agent, the oil control agent comprises a Thermus thermophilus fermentation product, the mass percentage content of the Thermus thermophilus fermentation product in the oil control agent is not less than 80%; the 10-hydroxydecanoic acid, the sarcosine and the oil control agent are combined according to the specific mass parts to obtain the composition with an excellent oil control effect, and the composition can effectively inhibit 5 alpha-reductase in sebaceous gland cells, reduce conversion of testosterone to dihydrotestosterone (DHT), inhibit sebaceous gland secretion and reduce grease accumulation on the skin surface, so that the sebaceous gland skin-care effect is improved, and the skin-care effect is improved. Pore blockage is reduced, the number of active sebaceous gland bodies is reduced, the secretion activity of the active sebaceous gland bodies is reduced, and skin grease is effectively reduced.

The application relates to the technical field of cell culture, and particularly discloses a culture medium for musk deer musk gland cells and a culture method for musk deer musk gland cells. The culture medium for the musk deer musk gland cells comprises a DMEM / F12 culture medium and the following components: 80-120 U / mL penicillin, 0.08-0.12 mg / mL streptomycin, 8-12% fetal bovine serum, 40-60 ng / mL epidermal growth factor, 80-120 ng / mL fibroblast growth factor, 0.06-0.15 ug / mL hydrocortisone, 0.4-0.6 uM / mL TGF-beta inhibitor, 1.6-2.4 mM / mL glutamine and 3.5-6 mM / mL acetyl-L-cysteine. The application further provides a culture method for the musk deer musk gland cells, which comprises the following steps: digestion, cell preparation, primary culture and subculture. The culture medium for the musk deer musk gland cells is used to culture the musk deer musk gland cells, the subculture effect of the musk deer musk gland cells is good, the proliferation capacity of the musk deer musk gland cells is strong, the morphological change of the musk deer musk gland cells is small, and the musk deer musk gland cells can be well kept dry during the subculture.

The invention relates to the technical field of cell engineering and biopharmacy, and discloses a method for promoting synthesis of steroids in forest musk gland cells, which comprises the following steps: determining the tolerance range of cholesterol concentration through a pre-experiment, setting an experimental group, culturing cells, collecting and treating a sample, and detecting by LC-MS (Liquid Chromatography-Mass Spectrometry). According to the method for promoting synthesis of the steroids in the lindel gland cells, the synthesis amount of the seven steroids in the lindel gland cells can be up-regulated by regulating the cholesterol concentration of the culture medium to 10-20 mg / L, the content of pregnenolone is up-regulated by 4.12 times (Plt, 0.05), the content of progesterone is up-regulated by 1.46 times (Plt, 0.05), and the method is remarkably superior to that of existing culture without cholesterol regulation; a complete standardized process from pre-experiment, culture, sample treatment to detection is provided, the experiment result repeatability is high, and the method can be directly applied to industrial production.

The application discloses a method for culturing sweat gland cells to obtain a sweat gland organoid and application thereof, and belongs to the technical field of bioengineering, wherein the sweat gland cells can be cultured by using a three-dimensional culture medium provided by the application, and a sweat gland organoid with high cell activity and bidirectional differentiation potential is obtained; after being transplanted into a full-thickness injury mouse on the sole and back, the sweat gland organoid not only can promote the regeneration and function recovery of the sweat gland, but also can promote the healing of the skin wound, and can be used for preparing a cell product for repairing skin injury and recovering the function of an accessory organ, and has a wide application prospect.

This invention provides the application of NDV in the preparation of drugs for treating hyperthyroidism, belonging to the field of pharmaceutical technology. This invention discovers that the cytotoxic effect of NDV is significantly time-dependent, with the killing effect gradually increasing with prolonged culture time. Furthermore, this invention is the first to discover that NDV can significantly enhance the cytotoxic ability of JAK inhibitors against thyroid follicular epithelial cells, effectively inhibit the growth of overactive thyroid cells, and induce apoptosis. This not only overcomes the limitation of JAK inhibitors alone in killing thyroid epithelial cells, but also reduces the cells' natural resistance to NDV by inhibiting the JAK-STAT pathway, thus strengthening the lytic effect of oncolytic viruses on hyperactive thyroid cells, providing a novel drug combination strategy with a synergistic mechanism for the treatment of hyperthyroidism.

The invention discloses an application of sesquiterpenoids in preparation of drugs for treating thymus injury, and particularly relates to an application in preparation of drugs for treating acute thymus injury, an application in regulation of secretion of inflammatory factors in thymus cells and subgroups thereof and an application in increase of the number of immune cells. Wherein the extract of the sesquiterpenoids is a ligularia virgaurea seed, and is identified to be the Irimophenane type sesquiterpenoids. The medicinal value of the sesquiterpenoids WH-1 is further studied, and it is found that the sesquiterpenoids WH-1 can enhance the inflammatory iTECs cell viability, improve the inflammatory iTECs function and relieve acute thymus injury, so that the organism immunity is improved.

This application relates to the technical field of cell culture, specifically disclosing a culture medium for forest musk gland cells, its preparation method, and its application. The culture medium disclosed in this application uses DMEM / F12 as the basal medium and contains the following components as functional additives at various concentrations: penicillin 80-120 U / ml, streptomycin 0.08-0.12 mg / ml, FBS 8-12 v / v%, non-essential amino acids 0.7-1.3 v / v%, growth factors 20-40 ng / ml, and vitamins 0.05-0.2 μg / ml. This application also discloses the preparation method of the above-mentioned culture medium and its application in the culture of forest musk gland cells. When forest musk gland cells are cultured using the culture medium disclosed in this application, the cell proliferation rate is faster, the cell activity is stronger, and the cell adhesion rate is higher.

The invention belongs to the technical field of medicinal chemistry, and particularly discloses a platinum (IV) prodrug with ferroptosis and apoptosis induction effects as well as a preparation method and application of the platinum (IV) prodrug. According to the invention, a novel platinum (IV) compound with a ferroptosis induction effect is developed based on platinum (IV) prodrugs of salazosulfapyridine, cis-platinum and carboplatin; the platinum (IV) prodrug disclosed by the invention shows excellent anti-proliferative activity to cisplatin-sensitive and drug-resistant triple negative breast cancer cells, and compared with cisplatin, the platinum (IV) prodrug has lower activity to a normal mammary gland cell line (MCF-10A). Accumulation of reactive oxygen species (ROS) in cells can be improved by inducing DNA damage, and a mitochondrial dependent approach is activated to induce apoptosis. Meanwhile, accumulation of lipid peroxide can be remarkably promoted, xCT and GPX4 levels are reduced, and mitochondrial morphological change is induced to stimulate ferroptosis; in vivo, the cis-platinum (IV) prodrug shows strong anti-tumor activity in an MDA-MB-231 / CDDP xenograft model, and the cis-platinum (IV) prodrug has strong anti-tumor activity; the platinum (IV) prodrug has a potential application prospect in targeted therapy of triple negative breast cancer.

The invention relates to the field of traditional Chinese medicine modern pharmacy, and discloses application of leonurine in preparation of a product for regulating sebum secretion of sebaceous gland cells, and the product for regulating sebum secretion of sebaceous gland cells comprises a sebum overflow disease medicine composition and an oil-control skin care product composition. The traditional Chinese medicine is expected to serve as a safe, effective and long-term-maintenance new treatment mode for sebum overflow diseases. The leonurine can inhibit the synthesis of sebum by inhibiting the gene transcription level and protein expression level of SREBP1 and FASN, thereby playing a role in treating sebum overflow diseases such as seborrheic dermatitis, acne and the like.

The invention discloses an induced differentiation method of sebaceous gland cells, which comprises the following specific steps: adding epidermal stem cells into an induction culture medium, and carrying out induced culture for 5-7 days to obtain the sebaceous gland cells, wherein the adopted induction culture medium is not added with insulin and specifically comprises the following components: 50% of a keratinocyte culture medium, 50% of a sebaceous gland cell culture medium, 1% of antibiotics, 20-40 ng / mL of EGF, 10-40 ng / mL of KGF and 5-20 ng / mL of rosiglitazone. Growth factors EGF and KGF and rosiglitazone are simultaneously added into the induction culture medium, insulin is completely not added, normal division and proliferation of cells are guaranteed, and sebaceous gland cells which are good in growth state and high in differentiation degree are obtained in a short culture period; compared with SZ95, the expression quantities of various sebaceous gland cell markers are similar.