52 results about "Gland cell" patented technology

The invention discloses a mammary gland immunohistochemical Ki-67 image segmentation method based on an attention mechanism, and the method comprises the following steps: obtaining a mammary gland immunohistochemical Ki-67 image, and carrying out the segmentation processing of the mammary gland immunohistochemical Ki-67 image, and obtaining mammary gland immunohistochemical Ki-67 image blocks; taking the breast immunohistochemical Ki-67 image block as input, taking the corresponding cell type classification result as output, and training a pre-constructed breast cell segmentation model; a mammary gland cell segmentation model is constructed based on a VGG16 network and a cooperative attention mechanism of a space and a channel; segmenting a mammary gland immunohistochemical Ki-67 image to be predicted into a plurality of image blocks, and inputting the image blocks into the trained mammary gland cell segmentation model for segmentation; and obtaining a distribution density map of classification results of different types of cells. The invention relates to the technical field of image segmentation, and by coordinating the relationship between spatial features and channel features and enhancing the guiding capability of the features, semantic feature differences are effectively relieved, and the precision, flexibility and adaptability of tumor cell segmentation are remarkably improved.

The invention belongs to the field of biological medicine, and provides circular RNA for treating multiple endocrine tumor type I and application of the circular RNA. According to the invention, after different optimization and design are carried out on the circular RNA for coding Menin, the circular RNA with relatively good expression level and long half-life period is finally obtained, and the anti-tumor effect is mainly achieved by supplementing cell-deficient Menin. Compared with the prior art, the circular RNA for expressing the Menin protein provided by the invention is more stable in intracellular expression and longer in half-life period. In an immortalized parathyroid gland cell model, the Menin protein can be expressed by transfecting the circular RNA for 6 hours, and the circular RNA has good treatment potential for multiple endocrine tumor type I.

The application relates to a kind of gland cell image segmentation methods based on selective multi-branch hollow convolution, input gland cell image dataset, with improved UNet as main network, construct gland cell segmentation network based on selective multi-branch hollow convolution, the network is captured more advanced features with context encoding network, with channel attention module adaptive recalibration channel feature response and highlight the most relevant feature channel, with multi-scale selection module amplifies effective information and suppresses redundant information, selects the appropriate scale of hollow convolution branch;With the input gland cell image dataset, the segmentation network is trained to obtain a stable segmentation network;Input to be segmented gland cell image, with stable segmentation network to carry out gland cell segmentation.The application has better generalization ability, and is especially suitable for the size, shape change difference of gland in colorectal polyp.

The application discloses application of a reagent for detecting LRRN3 expression level in preparation of a benign prostatic hyperplasia diagnosis product. It is proved through cell function experiments and animal experiments that overexpression of the LRRN3 gene can inhibit the proliferation of prostate cells, block the cell cycle and promote cell apoptosis, thereby effectively inhibiting the prostatic hyperplasia. The discovery provides a new target and treatment strategy for the treatment of the prostatic hyperplasia, and provides a brand-new thought and method for the treatment of the prostatic hyperplasia. Based on the research results, a gene therapy drug taking the LRRN3 gene as a target can be further developed, for example, a lentivirus vector carrying the LRRN3 gene or other gene delivery systems are constructed and used for the treatment of the prostatic hyperplasia. In addition, the LRRN3 gene can be combined with other treatment methods to improve the treatment effect.

Disclosed herein are compositions and methods related to differentiation of stem cells into pancreatic endocrine cells. In some aspects, the methods provided herein relate to generation of pancreatic β cell, α cell, δ cells, and EC cells in vitro. In some aspects, the disclosure provides pharmaceutical compositions including the cells generated according to the methods disclosed herein, as well as methods of treatment making use thereof.

The invention discloses a protein complex for protecting mitochondria from being damaged, a pharmaceutical composition and application. According to the application, the influence of nitrate on mitochondrial injury in in-vivo radiation-induced salivary gland injury and in-vitro human salivary gland cell injury is evaluated, the action mechanism in the mitochondrial injury is further deeply explored, and a result shows that sialic acid transporter interacts with FKBP8, so that the mitochondrial injury can be inhibited, and the mitochondrial injury can be inhibited. In turn, particles are formed and anchored on the outer mitochondrial membrane, thereby maintaining membrane integrity. Therefore, the invention provides a brand new therapeutic target for preventing radiation-induced salivary gland injury and other mitochondrial membrane related diseases.

The utility model relates to breast nursing technical field especially a breast nursing dredging device, including handle, the surface screw thread connection of handle has the cover cup, the inner chamber of cover cup is provided with the massage head, the inner chamber of cover cup is provided with the adjusting mechanism, the adjusting mechanism includes the massage block, the massage block swing setting is in the inner chamber of cover cup, the surface of massage block is connected with the cannula, the surface of handle is provided with the liquid adding pipe, one end of liquid adding pipe is connected with the connecting pipe, the utility model discloses through the cooperation of massage head, massage block, cannula, liquid adding pipe, connecting pipe, ring pipe, liquid warehouse pipe, driving motor, sliding mouth and ball, is equipped with rolling massage head and sliding mouth, can add the liquid of alleviating automatically in the massage process, utilizes driving motor to drive the ball rolling massage and liquid alleviating combination, can more deeply stimulate breast cell, promotes blood and lymphatic fluid circulation, accelerates the dredging and recovery of breast.

The invention belongs to but not limited to the technical field of biomedicine, and discloses a composition with a cell protection effect and application thereof, the composition comprises five monomers of cycloastragenol, resveratrol, ginsenoside, tetrahydrocurcumin and cellulose, and can also comprise a moisturizing composition and a moisturizing composition. The composition provided by the invention can accurately act on vaginal smooth muscle cells and gland cells, and can be used for recovering or improving endocrine balance by improving cell activity, promoting elastin synthesis and repairing cell injury in multiple ways, improving telomerase activity of a reproductive system and regulating immune response, so that the curative effect of the composition is improved. Various injury factors such as exogenous factors, traction of production machinery and age increase are effectively resisted, and the method has wide application prospects in the fields of postpartum reproductive system rehabilitation, maintenance of normal functions of the reproductive system, prevention and improvement of conditions such as vagina relaxation, dryness and poor injury repair and the like, and prevention and treatment of related diseases of the reproductive system and health care.

The present invention relates to a peptide conjugate having anti-diabetic activity and uses thereof. The peptide conjugate of the present invention exhibits blood glucose level lowering efficacy by promoting glucose absorption into cells, suppressing insulin resistance signals, promoting insulin sensitivity signals, and suppressing the death of pancreatic β cells, which are insulin-producing cells. The present invention also relates to a peptide having anti-diabetic and anti-obesity activities and uses thereof. The peptide of the present invention has activities of suppressing insulin resistance signals, promoting insulin sensitivity signals, and promoting fat breakdown in adipocytes.

The invention belongs to the technical field of experimental animal models, biomedical research and immunology, and discloses a photocoagulation thymus ischemia animal model, a method and application of the photocoagulation thymus ischemia animal model in rapid simulation of thymus degeneration and immune senescence, and the model shows at least one or more characteristics 1-7 days after induction: thymus atrophy, thymus weight or thymus index is significantly reduced; the cortical-medullary structure of thymus is disordered or disappears; the expression distribution of the Keratin 5 / Keratin 8 is abnormal; thymic cell apoptosis is increased, and lipid deposition is enhanced; the proportion of CD4 + CD8 + double positive T cells is reduced, and the proportion of DN2 / DN3 stage T cells is increased; the initial T cell output is reduced; and the immune function is reduced. The pathological mechanism is clear; the modeling period is short, and the immune senescence phenotype can be stably obtained within several days; the injury focus is controllable, and systemic stress or hormone interference is avoided; the model has high repeatability and is suitable for mechanism research and drug screening.

Methods and compositions of matter for production of pancreatic beta cells, as well as beta-like cells that are capable of producing insulin. Pluripotent stem cells are generated through either viral vector or non-viral vector means and induced into differentiation pathways through environments resembling the pluripotent pancreatic environment. In vivo transfection of dedifferentiation and beta cell differentiation genes are provided in vivo in the pancreatic microenvironment to generate de novo beta cells and islets from differentiated tissue or islet progenitors. Means of inducing differentiation of islet progenitors in vivo are also provided.

Provided is a method of differentiating human pluripotent stem cells (hPSCs) into mature pancreatic β cells, comprising contacting hPSC-derived, differentiated immature pancreatic β cells with a histone deacetylase (HDAC) inhibitor.

The invention relates to the field of biology, in particular to an in-vitro separation, culture and identification method of human primary sweat gland muscle epithelial cells. The invention aims to solve the problems in the background technology, and provides an in-vitro separation, culture and identification method of human primary sweat gland epithelial cells, which is characterized by comprising the following steps: acquiring a skin specimen, preserving at low temperature, cleaning, digesting, cleaning, digesting, irradiating and disinfecting, adhering an implant to the wall, separating and planting, abandoning a culture medium, and observing and photographing. The specific operation modes are as follows: (1) acquiring a skin specimen: acquiring the skin specimen through an operation; and (2) low-temperature preservation: putting into a sterile PBS (Phosphate Buffer Solution) containing double antibodies of 1 * penicillin (100U / ml)-streptomycin (0.1 g / ml) for low-temperature preservation. The method disclosed by the invention has the beneficial effects that sweat gland cells are cultured by utilizing a sweat gland tissue block adherent method, sweat gland muscle epithelial cells are obtained and separated by using pancreatin digestive juice at a time point when the cells grow for one week, and in addition, the cells are different in adherent capability.

The present disclosure describes a method for preventing or treating an abnormal mammary gland cytopathy characterized by cell proliferation in a subject. The method comprises administering to the subject an effective amount of an androgen agent in combination with an effective amount of an aromatase inhibitor, increasing the concentration of the androgen agent in serum to a level to increase the intramammary concentration of androgen in the mammary gland resulting from the action of 5 alpha-reductase on the androgen agent, the increased intramammary concentration of the androgen induces inhibition of c-Myc and / or mTOR expression or activity in the mammary gland associated with a lower intramammary concentration of the androgen. The increase in the serum concentration of the androgen agent and the increase in the intramammary concentration of the androgen are maintained for a predetermined period of time without substantially increasing the concentration of the androgen in the serum of the subject. Furthermore, the use of the aforementioned androgen and aromatase inhibitors for reducing the risk of breast cancer progression to hormone-resistant breast cancer, reducing the risk of HER2-positive breast cancer becoming insensitive to targeted HER-2 anti-cancer drug therapy, and for inducing inhibition of c-Myc and / or mTOR expression and / or activity is also described. In embodiments, the androgen agent may be testosterone and androgen 5 [alpha]-dihydrotestosterone (DHT).

The invention belongs to the field of medicine preparation, and relates to application of a CPT1A inhibitor in preparation of a sepsis medicine. By constructing a mouse cecum ligation perforation sepsis model, it is found that the number of thymus DP cells is remarkably reduced after 24h of CLP operation, and T cell subpopulation distribution is abnormal. The loss of the DP cells further leads to hindering of output of newborn T cells, and finally the number of T cells in the spleen and peripheral blood is remarkably reduced. A CPT1A inhibitor Etomoxir is used for intervention, and the rescue effect of the CPT1A inhibitor Etomoxir on mitochondrial functions, cell apoptosis and T cell subset distribution is verified. Experimental results show that CPT1A plays an important role in the death process of thymus T cells caused by sepsis and is expected to become a target spot for treating sepsis in the future.

The invention relates to the technical field of medicine preparation, in particular to application of a serine protease inhibitor in preparation of medicine for treating hyperhidrosis, animal experiments prove that the serine protease inhibitor can inhibit sweat gland cell activity, new use of old medicine is achieved, sweat gland secretion is improved, and the effect of treating hyperhidrosis is achieved. And a new scheme is provided for development of drugs for treating the polyhamia.

The invention belongs to the field of biological medicines, and provides mRNA (messenger ribonucleic acid) for treating multiple endocrine tumor type I and application thereof. The mRNA provided by the invention has high specific expression level in human cells such as 293T, parathyroid gland and the like; in addition, the mRNA provided by the invention can be continuously expressed on SK-BR-3 cells for at least 7 days, and the half-life period of the protein is longer. The mRNA provided by the invention plays an anti-tumor role mainly by supplementing cell-deficient Menin protein, in an immortalized parathyroid gland cell model, the Menin protein can be expressed by transfecting the mRNA for 6 hours, and the mRNA has a good treatment potential for multiple endocrine tumor type I.

The application discloses a preparation method of a 3D model of a maternal-fetal interface constructed by an endometrial organoid in vitro, and the method comprises the following steps: establishing a primary endometrial epithelial organoid and an endometrial stromal cell line in vitro; improving an extracellular matrix for assembling endometrial gland-like organoids and stromal cells; the constructed endometrial organoid model comprises gland cells and stromal cells; the endometrial organoid model is cultured by using a gas-liquid interface, so that the endometrial organoid model has a cavity epithelium and a gland structure; the endometrial organoid model simulates a menstrual cycle of the endometrium and performs function identification. The endometrial organoid model has the cavity epithelium and the gland epithelium for the first time, the tissue structure, the cell composition, the hormone-induced change, the gene expression characteristics of the organoid are similar to those in the body, and the organoid can reproduce the receptivity genes and the dynamicity of cilia which are necessary for embryo implantation in the body; the organoid can be used as a model for studying embryo implantation regulation, endometrial diseases and regeneration mechanisms.

This invention relates to the field of pharmaceutical biotechnology, providing the application of aminoquinoline compounds (such as hydroxychloroquine and chloroquine) in the preparation of drugs for treating hyperparathyroidism. These compounds restore the expression of calcium-sensitive receptors (CaSRs) on the cell membrane surface by inhibiting the lysosomal degradation pathway of CaSRs in parathyroid cells. Experiments have confirmed that 4-aminoquinoline compounds, used alone or in combination with calcimimetics (such as cinacalcet), significantly reduce serum PTH and calcium levels in a model of refractory secondary hyperparathyroidism (SHPT) and inhibit glandular hyperplasia. In particular, the combination therapy regimen exhibits a significant synergistic effect, effectively reversing calcimimetics resistance. This invention is the first to discover a novel use for the antimalarial / antirheumatic drug hydroxychloroquine in the treatment of hyperparathyroidism, realizing a new use for an existing drug; and providing a safe and effective new drug treatment strategy for clinically refractory SHPT.

The invention discloses a thermophilic thermophilic bacteria fermentation product, a preparation method and application thereof and an oil control product, and belongs to the technical field of cosmetics. Thermophilic thermophilic bacteria are cultured in a first-stage seed solution culture medium and a second-stage seed solution culture medium in sequence, the thermophilic thermophilic bacteria are promoted to the logarithmic phase, then a mulberry leaf extracting solution is added for fermentation, and the oil control product is obtained. According to the method, the growth stability of thalli is effectively prolonged, the stability of a fermentation microenvironment is maintained, the metabolic activity of the thermophilic thermophilic bacteria is improved, normal replication of thallus DNA at high temperature can be guaranteed, and the thermophilic thermophilic bacteria can grow at a high speed in the whole growth period. According to the method disclosed by the invention, the accumulation of byproducts can be avoided, active ingredients with an oil control effect in the thermophilic thermophilic bacteria are effectively enriched, and a thermophilic thermophilic bacteria fermentation product which has the effects of improving the mRNA expression quantity of AMPK, effectively inhibiting the activity of 5 alpha-reductase, inhibiting sebaceous gland cells from synthesizing lipid droplets and reducing hormone-induced lipid synthesis is obtained; the oil control activity is excellent.

The invention relates to application of Antarctic red in a skin conditioning agent for synergistically controlling oil and resisting wrinkles, and belongs to the technical field of biology, the Antarctic red has no toxicity to cells when the concentration is not higher than 250 g / mL, gene expression of I-type 5 alpha-reductase in sebaceous gland cells is inhibited in a targeted mode, and excessive synthesis of grease is reduced from the source; the gene expression level of I type and III type collagen in dermal fibroblasts is synchronously improved, so that the internal support structure of the skin is reconstructed, and the anti-wrinkle effect is realized. According to the Antarctic red disclosed by the invention, through the synergistic effect of'descending and ascending ', the Antarctic red is proved to be capable of simultaneously and accurately intervening an'oil-wrinkle' associated pathway, and the limitation of a single functional component is broken through.

Disclosed herein are compositions and methods related to differentiation of stem cells into pancreatic islet cells. In some aspects, the methods provided herein relate to generation of pancreatic β cell, α cell, δ cells, and EC cells in vitro. In some aspects, the disclosure provides pharmaceutical compositions including the cells generated according to the methods disclosed herein, as well as methods of treatment making use thereof.

Embodiments of the present disclosure relate to novel compositions and methods for generating thymic cells. In certain embodiments, thymus cells can be differentiated from pluripotent stem cells (PSC), anterior primitive stripe (APS) cells, definitive endoderm (DE) cells, anterior intestinal endoderm (AFE) cells, pharyngeal endoderm (PE) cells, abdominal pharyngeal endoderm (VPE) cells, and third pharyngeal sac endoderm (TPPE) cells using the compositions and methods disclosed herein. In certain embodiments, thymic cells generated by the compositions, systems, and methods disclosed herein can be used to treat health conditions.

The invention discloses a culture medium and a method for constructing an immune organ-like model and application, belongs to the field of biological medicines, and mainly solves the problem that in-vitro human immune organ model construction and human immunology process simulation are difficult to realize. According to the method, human peripheral blood mononuclear cells (PBMC), thymus cells, tonsil cells, spleen cells or lymph node primary cells are inoculated in a culture plate with a specific structure, and an immune environment with a physiological three-dimensional (3-dimensional) structure is formed by applying a specific culture medium and conditions, so that long-term, efficient and convenient culture of the immune organ is realized; the immune tissue function of a human body is simulated. The technology involved in the invention can be applied to the fields of deep research of accelerated immunology mechanism, drug screening and evaluation, patient selection, disease detection and the like.

The invention belongs to the field of biological medicine, and particularly relates to application of acidic tripeptide to preparation of drugs for resisting viruses and virus diseases of aquatic animals. The acidic tripeptide Tripeptide is a polypeptide which is composed of three amino acids, namely, Leu, Asp and Val. The application has the advantages that firstly, the key effect of the VP7 in virus infection is verified from the positive and negative aspects, the infection of grass carp reovirus (GCRV) can be promoted by transfecting VP7 plasmids to grass carp gonad cell lines (GCO), and the GCRV infection can be inhibited by blocking VP7 antibodies; and (2) in-vivo and in-vitro experiments prove that GCRV virus infection can be effectively inhibited by applying the acidic tripeptide from the VP7 to treat. And (3) the invention discloses a brand new biological function of the virus outer capsid structure protein source Tripetide, and effectively blocks the virus from invading cells, thereby reducing host infection. The tripeptide is non-toxic to grass carp cells in an effective concentration range. The research provides important theoretical and experimental basis for developing efficient and safe drugs for resisting viral diseases of aquatic animals.

The invention discloses application of furochromone in preparation of a medicine for treating triple negative breast cancer. The invention relates to novel application of furochromone in treatment of triple negative breast cancer, research shows that furochromone can inhibit cell proliferation and migration of triple negative breast cancer, can significantly inhibit tumor growth of TNBC, reduce tumor size and weight and relieve symptoms of model animals, has a treatment effect superior to that of positive drugs, and has in-vivo safety. Meanwhile, the furochromone is relatively low in toxicity to normal mammary gland cells, and relatively high in selectivity and safety. Therefore, a foundation is laid for developing more medicines for treating the triple-negative breast cancer, the furochromone becomes a new choice of the medicines for treating the triple-negative breast cancer, and the furochromone has important significance for treating the triple-negative breast cancer.

The invention discloses an oil control composition for regulating sebaceous glands and a preparation method and application thereof, and belongs to the technical field of cosmetics, the oil control composition for regulating sebaceous glands comprises the following components in parts by mass: 0.5-10 parts of 10-hydroxydecanoic acid, 5-25 parts of sarcosine and 5-50 parts of an oil control agent, the oil control agent comprises a Thermus thermophilus fermentation product, the mass percentage content of the Thermus thermophilus fermentation product in the oil control agent is not less than 80%; the 10-hydroxydecanoic acid, the sarcosine and the oil control agent are combined according to the specific mass parts to obtain the composition with an excellent oil control effect, and the composition can effectively inhibit 5 alpha-reductase in sebaceous gland cells, reduce conversion of testosterone to dihydrotestosterone (DHT), inhibit sebaceous gland secretion and reduce grease accumulation on the skin surface, so that the sebaceous gland skin-care effect is improved, and the skin-care effect is improved. Pore blockage is reduced, the number of active sebaceous gland bodies is reduced, the secretion activity of the active sebaceous gland bodies is reduced, and skin grease is effectively reduced.

The present invention relates to a novel use of a cosmetic composition comprising 10-hydroxystearic acid, a salt, ester or amide thereof for topical application to the skin to modulate lipid balance in sebaceous gland cells, modulate sebaceous gland cell activity and / or inhibit a lipid production process induced by an inducer.

The application relates to the technical field of cell culture, and particularly discloses a culture medium for musk deer musk gland cells and a culture method for musk deer musk gland cells. The culture medium for the musk deer musk gland cells comprises a DMEM / F12 culture medium and the following components: 80-120 U / mL penicillin, 0.08-0.12 mg / mL streptomycin, 8-12% fetal bovine serum, 40-60 ng / mL epidermal growth factor, 80-120 ng / mL fibroblast growth factor, 0.06-0.15 ug / mL hydrocortisone, 0.4-0.6 uM / mL TGF-beta inhibitor, 1.6-2.4 mM / mL glutamine and 3.5-6 mM / mL acetyl-L-cysteine. The application further provides a culture method for the musk deer musk gland cells, which comprises the following steps: digestion, cell preparation, primary culture and subculture. The culture medium for the musk deer musk gland cells is used to culture the musk deer musk gland cells, the subculture effect of the musk deer musk gland cells is good, the proliferation capacity of the musk deer musk gland cells is strong, the morphological change of the musk deer musk gland cells is small, and the musk deer musk gland cells can be well kept dry during the subculture.

The invention relates to the technical field of cell engineering and biopharmacy, and discloses a method for promoting synthesis of steroids in forest musk gland cells, which comprises the following steps: determining the tolerance range of cholesterol concentration through a pre-experiment, setting an experimental group, culturing cells, collecting and treating a sample, and detecting by LC-MS (Liquid Chromatography-Mass Spectrometry). According to the method for promoting synthesis of the steroids in the lindel gland cells, the synthesis amount of the seven steroids in the lindel gland cells can be up-regulated by regulating the cholesterol concentration of the culture medium to 10-20 mg / L, the content of pregnenolone is up-regulated by 4.12 times (Plt, 0.05), the content of progesterone is up-regulated by 1.46 times (Plt, 0.05), and the method is remarkably superior to that of existing culture without cholesterol regulation; a complete standardized process from pre-experiment, culture, sample treatment to detection is provided, the experiment result repeatability is high, and the method can be directly applied to industrial production.