The invention relates to a primer, a probe and a kit for distinguishing
sansevieria glauca and
sansevieria clavata. The
nucleotide sequence of the
DNA probe is as shown in SEQ ID NO.1. The invention also relates to a kit for distinguishing
sansevieria glauca from sansevieria clavata. The primers comprise 1 to 2k-F and 1 to 2k-R; the
nucleotide sequence of the primer 1-2k-F is as shown in SEQ ID NO. 2; the
nucleotide sequence of the primer 1-2k-R is as shown in SEQ ID NO.3. The kit comprises the probe and the primer. A PCR amplification product is subjected to
Sanger sequencing, sequencing results are compared, the comparison result shows that a 23 bp
insertion site exists at the site of sansevieria glauca and sansevieria barbata, and the sansevieria glauca and sansevieria barbata can be distinguished, and an
oligonucleotide fluorescent probe is designed according to the site, so that the detection sensitivity is improved, and the detection accuracy is improved. Therefore, the sansevieria
valeriana and the sansevieria glauca can be reliably and effectively distinguished.