524 results about "Leaf lamina" patented technology

The invention relates to the technical field of ecology and hydrology, in particular to a terrestrial ecosystem water carbon flux estimation method considering water balance constraint, which comprises the following steps: estimating leaf scale photosynthetic rate and stomatal conductance by utilizing a photosynthesis model; the leaf scale photosynthetic rate and the stomatal conductance are upscaled into canopy vegetation total primary productivity and canopy conductance to construct an evapotranspiration model to estimate evapotranspiration data; constructing a water balance model according to the evapotranspiration data so as to estimate the available water amount of the soil; constructing a soil moisture stress function according to the available water amount of the soil so as to constrain the estimation process of the photosynthesis model and the evapotranspiration model; and acquiring historical water-carbon flux data and historical remote sensing observation data of a long sequence daily scale, and calibrating the model to obtain a water-balance-constraint-considered water-carbon flux estimation model of the terrestrial ecosystem capable of estimating the water-carbon flux data. Therefore, the problems that an existing water carbon flux prediction frame is difficult to adapt to climate changes, low in prediction accuracy and the like are solved.

The invention discloses a monitoring method and system for identifying crop drought based on leaf morphology, and relates to the field of agricultural drought monitoring, and the method comprises the following steps: selecting a completely unfolded healthy leaf, cleaning with ultrapure water, blow-drying with nitrogen, and fixing on a standard objective table to obtain a standardized leaf sample; based on the standardized leaf sample, a high-resolution scanner is used for obtaining a front gray image and a back gray image of the leaf, and a binary image with a clear vein network is obtained through image splicing and binarization processing; skeletonizing the binary image with the clear vein network, and calculating the vein fractal dimension by adopting a box counting method to obtain a fractal dimension change rate; the method is in a fixed detection area of a standardized leaf sample. Through innovative leaf vein fractal dimension analysis and contact angle dynamic measurement, a crop drought monitoring system is constructed, and in the aspect of leaf vein analysis, skeletonization processing and a box counting method are adopted to realize accurate quantification of a leaf vein network topological structure.

The invention relates to the technical field of photosynthetic carbon sequestration, in particular to a photosynthetic carbon sequestration data real-time monitoring system and method, and the system comprises an evapotranspiration gradient recognition module, a photosynthetic rate change capture module, a non-cooperative state judgment module, a carbon sequestration fluctuation trajectory extraction module and a monitoring stability output module. According to the method, the response time point of the evapotranspiration process to the illumination change is dynamically positioned by obtaining parameters such as stomatal conductance, leaf surface temperature, saturation vapor pressure difference and transpiration rate of a leaf layer, the time point of sudden change of the physiological activity is marked, and the change of the transpiration rate and the change of the net photosynthetic rate are compared according to the difference range between the change of the net photosynthetic rate and the change of the transpiration rate. According to the method, the non-collaborative evapotranspiration function state can be judged, the possible response imbalance situation between leaf photosynthesis and moisture adjustment can be effectively recognized, the recognition capacity for the dysfunction time period is enhanced, the time window with low output stability is screened, and the dynamic adaptability and abnormal response judgment capacity of carbon sequestration monitoring data are enhanced.

The invention relates to the technical field of molecular markers, in particular to an Indel molecular marker related to yellow leaves of a melon seedling growing point and application of the Indel molecular marker. The invention explores a key candidate gene MELO3C016136 for controlling the leaf color of the growth point of the muskmelon seedling, and develops an Indel molecular marker ygp2 for identifying the leaf color change of the growth point of the muskmelon seedling based on the key candidate gene MELO3C016136. The molecular marker ygp2 can be used for identifying the leaf color change character of the growth point of the muskmelon seedling, only one-time PCR amplification and polyacrylamide gel electrophoresis need to be carried out on the DNA of the plant to be detected, the identification result is reliable, the genotype of the target plant can be simply and conveniently identified, and the genotype identification result is completely consistent with the leaf color phenotype of the growth point of the seedling; important technical support is provided for creating new muskmelon germplasm with yellow seedling growing point leaves.

The invention relates to the technical field of image acquisition, in particular to an insect pest image acquisition method for vegetable insect pest prevention and control, which comprises the following steps: accurately extracting a single leaf in a leaf image through vein features of vegetable leaves, and obtaining a complete leaf and a sheltered leaf; then determining an insect pest gnawing area in the single leaf based on color characteristics of leaf meat in the single leaf and continuity of leaf veins; based on the number and area characteristics of pest gnawing areas, the pest gnawing degrees of the complete leaves and the sheltered leaves are quantified; and finally, according to different gnawing degrees of insect pests, different image acquisition modes are adopted for different leaves. By means of the method, more accurate positioning and collection of the pest gnawing area can be achieved, detection errors caused by leaf shielding in a traditional method can be avoided, and more targeted data support is provided for follow-up pest gnawing degree evaluation and prevention and control scheme making through dynamic allocation of resources.

A sweet potato leaf in-situ instantaneous transformation method comprises the following steps that sweet potato stem segments are sheared and cut into a matrix or water, and the sweet potato stem segments are placed in a special incubator to be cultured till rooting; transforming a plasmid carrying a target gene into agrobacterium through a standard transformation program to obtain agrobacterium for infection, and adding the agrobacterium for infection into an infection solution to obtain an agrobacterium infection working solution; the method comprises the following steps of: pricking holes around veins of new leaves of a cultivated sweet potato plant, inversely putting the sweet potato plant into a vacuum flask filled with an agrobacterium infection working solution, completely immersing the leaves into the agrobacterium infection working solution, and treating for 40-50 seconds under the vacuum condition of 0.6-0.8 pa; and immediately transferring the infected plant into a turnover box for dark culture, and transferring the plant into a professional incubator for continuous culture after dark culture is finished, thereby obtaining the target gene introduced sweet potato transformed plant. The method can simplify the operation, shorten the time and improve the expression effect, and can be used for researching gene functions.

The invention belongs to the technical field of crop disease and insect pest recognition, and particularly discloses a crop disease and insect pest recognition analysis method based on image processing, and the method comprises the steps: collecting a state image of a plant in a dynamic environment, and eliminating the interference of the dynamic environment on the state image recognition of a plant leaf; differentiated analysis is carried out on disease and insect pest characteristics of different areas corresponding to each leaf area, so that leaf disease and insect pest types and defect data thereof are accurately identified; the color value distribution health state of leaves near a leaf vein distribution area is detected by simulating the leaf vein distribution contour of the interference area, the corresponding leaf vein structure stability of each leaf area is analyzed, and the leaf state of the remaining area after the interference area and the pest and disease damage area of the leaves are removed is identified. And assisting in evaluating the leaf risk area condition in each leaf area, and judging the pest and disease damage state in the plant leaf according to the condition. The recognition precision of the crop state image is improved, and targeted prevention and control measures can be taken.

The invention discloses a cuttage method for garden small chrysanthemum, and relates to the technical field of agricultural production. The method comprises the following steps: S1, selecting cutting slips: preferentially selecting top shoots and twigs, cutting the cutting slips with the length of 5-10cm, reserving 1-2 leaves at the top, cutting large leaves into halves, removing redundant leaves at the bottom, and cutting the large leaves into halves to reduce transpiration; s2, performing incision treatment and disinfection: obliquely cutting the base parts of the cutting slips by using a sharp cutter to increase the water absorption area, then soaking the cutting slips in a disinfectant for disinfection, and promoting rapid healing of the incision after disinfection. S3, rooting agent supplementation: soaking the bases of the cutting slips with a rooting agent to accelerate rooting; a loose and breathable mixed substrate is used, base fertilizer application is avoided, root burning is prevented, thorough watering is performed before cuttage, punching is performed with bamboo sticks, the rooting speed of the chrysanthemum can be effectively increased, and meanwhile the root quantity can be increased.

A method of fertilizing a plant including applying a composition to a root of the plant. The composition includes a spinel ferrite with a formula of MnxZnyFe2O4, where x+y=1. Particles of the spinel ferrite are spherical and have an average diameter of 10-20 nm. The plant has at least a 40% increase in leaf size compared to a plant under the same conditions but without applying the composition.

The invention relates to the technical field of plant genetic engineering, in particular to a transcription factor CsKNAT1 and application thereof in regulation and control of development of tea trees. The nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the sequence of protein coded by the gene is as shown in SEQ ID NO: 2. Research finds that overexpression of the CsKNAT1 gene can influence the form of plant leaves: the edges of the leaves are promoted to have notches (asymmetric leaves are formed, resulting in increase of sawteeth at the edges of the leaves); the formation of compound leaves can be promoted; leaves on plant stems can grow in a rosette form, and secondary stems are bolted in the middles of rosette leaves; the research also finds that the overexpression of the CsKNAT1 gene can obviously influence the flowering phase and the flower form of the plant; the delay of the flowering phase is promoted; overexpression of the CsKNAT1 gene can further influence development of floral organs and formation of siliques, part of arabidopsis plants have phenotypes that corolla becomes small, stamens are exposed, petals gradually extend and are zigzag in a wavy shape, the length of mature siliques is remarkably shortened by 20%-36.1%, and the phenomenon that the fruits are aborted occurs.

The invention provides a method for measuring the surface area of a kelp leaf and application thereof, and relates to the technical field of measurement of the area of the kelp leaf. The measuring method comprises the following steps: (1) shooting original data; (2) preliminary cutting and preprocessing of original data; (3) calibration plate detection; (4) kelp contour detection; and (5) calculating the basic phenotype of the kelp. According to the measuring method, the SAM model based on deep learning is adopted to segment the image, and the error can be close to or even smaller than that of manual measurement.

An MLC is provided, the MLC includes: a plurality of leaves; first driving mechanisms connected to the leaves and configured to drive the leaves to move to form a radiation field; and at least one leaf head mechanism mounted on at least one corresponding leaf of the leaves and configured to adjust the radiation field.

The invention discloses application of an MsMYB-ZD gene in regulating and controlling the leaf size and yield of medicago sativa, and belongs to the technical field of plant genetic engineering. According to the invention, an MsMYB-ZD transcription factor for regulating and controlling the leaf size of medicago sativa is cloned by using transcriptome analysis; experimental data show that the MsMYB-ZD gene presents a gradually up-regulated expression mode in a leaf development process; compared with wild alfalfa Gongnong No.1 1-16 single plants, the leaf area of the overexpressed MsMYB-ZD transgenic single plant is averagely enlarged by 77%, and the expression quantity and the leaf area of the transgenic line show a remarkable positive correlation relationship; the fresh grass yield of a transgenic single plant is increased by 158.7%, and the hay yield of a single plant is increased by 207.5%. The results show that the gene has important significance for improving the yield of medicago sativa.

The invention discloses a comprehensive evaluation method for saline-alkaline tolerance of medicago sativa, and relates to the technical field of plant saline-alkaline tolerance testing, by integrating soil salinity S and soil permeability P in a soil environment data set SED and actual salinity accumulation rate Rac and leaf moisture content W of a root system and a leaf in a growth and salinity accumulation data set PGSA, the saline-alkaline tolerance of the medicago sativa is evaluated, and the saline-alkaline tolerance of the medicago sativa is evaluated. Scientific evaluation is provided for the adaptability of medicago sativa in saline-alkali soil, the saline-alkaline tolerance score T of medicago sativa is made by predicting the salinity accumulation rate Rpr, then the saline-alkaline tolerance score T is compared with a preset saline-alkaline tolerance evaluation threshold value Tth, the adaptability of plants is judged, and targeted intervention suggestions are given according to the comparison result. According to the method, the actual planting data and the plant growth data of the medicago sativa after intervention are collected, the fluctuation trend of the data is analyzed, and the salt accumulation model is further iteratively optimized, so that the prediction accuracy and adaptability of the model are continuously improved.

The invention relates to the technical field of genetic breeding, and provides a BcKH14 gene for regulating and controlling growth of leaf epidermal hair of black cabbage, and the nucleotide sequence of the BcKH14 gene is as shown in SEQ ID NO.1. The invention also provides an overexpression vector containing the gene and a genetically engineered host cell. In addition, the invention also provides application of the gene in cultivation of a new variety of multi-epidermal black cabbage, and a method for promoting development of epidermal hair of black cabbage leaves. According to the invention, the key gene BcKH14 related to epidermal hair development is screened from the black cabbage, and the gene is over-expressed in an arabidopsis thaliana plant, so that the positive regulation of the gene on the epidermal hair of the leaf of the black cabbage is verified, the growth of the epidermal hair of the leaf of the plant can be promoted, and a foundation is laid for cultivating a new variety of the black cabbage with multiple epidermal hair; the method has important value for improving the stress resistance and disease and pest resistance of the black cabbage.

The embodiment of the invention provides a ray beam collimation system, and relates to the technical field of medical equipment. The ray beam collimation system comprises a primary collimator and a multi-leaf collimator. The primary collimator is provided with a through hole with a fixed shape and size, and the through hole is matched with the multi-leaf collimator in shape and size. And the primary collimator is positioned below the ray source and above the multi-leaf collimator. The multi-blade collimator comprises at least two blade groups composed of a plurality of blades, the tail end of each blade is provided with a concave-convex part, the concave-convex part comprises a plurality of first protrusions arranged at intervals in the first direction, a first recess is formed between every two adjacent first protrusions, the at least two blade groups comprise two blades opposite in the second direction, and the first protrusions are arranged at intervals in the first direction. The first protrusion of one of the two opposite blades is used for being connected with the first recess of the other blade in an inserted mode. The ray beam collimation system provided by the embodiment of the invention has the benefits that leakage rays between the tail ends of the blades are shielded, and the irradiation space of a patient in radiotherapy is increased.

The invention discloses application of a PdePin1At protein and a coding gene thereof in regulation and control of a plant tree form. According to the invention, a transgenic poplar with reduced PdePin1At gene expression quantity is constructed by using a microRNA interference technology, and experiments prove that compared with a wild poplar, the leaf area, the leaf width, the leaf length, the branch included angle, the petiole length and the phyllotactic angle of the transgenic poplar are reduced, and the number of leaves / stem nodes is increased. The method is of great significance to molecular mechanism and theoretical research of regulating and controlling poplar tree forms, leaf shapes and quantity, branch angles and phyllotaxis, and is of great guiding significance to directional cultivation of trees with tree forms meeting actual production requirements. Under the condition that the plant height is not changed, the number of leaves of newborn organs can be increased, so that the number of photosynthetic organs is increased; and by changing the included angle of the branches, sunshine shielding can be reduced during close planting of the forest trees. Therefore, the method also has important guiding significance on agricultural production.

This invention discloses a plant-based carrier material, its preparation method, and its application in skincare products. The plant-based carrier material is a powder of leaves from a Lamiaceae plant with an internal porous structure. This porous structure gives the material a good loading capacity and allows it to adsorb and slowly release small molecule liquid components. The preparation process of this carrier material is simple and mild. Furthermore, the Lamiaceae plant leaf raw material has antibacterial, antioxidant, and anti-inflammatory effects, making it suitable as a mask carrier. The cleansing mask obtained using this material as a carrier has excellent oil control and skin cleansing effects.

A new cultivar of Hydrangea macrophylla plant named ‘Hokomamipe’ that is characterized by its very dark green-black foliage, its inflorescences with sterile flower sepals that are clear white in color with light pink margins when mature. its sterile flower sepals with moderately serrated margins, and its compact plant habit.

The invention belongs to the field of long-stalked gynostemma pentaphyllum cuttings and provides a long-stalked gynostemma pentaphyllum cutting method and a cultivation device thereof. The cutting process comprises: selecting healthy and sturdy middle and upper stem segments of long-stalked gynostemma pentaphyllum in a planting field, placing the segments in a tray or a basket, and storing them in a cool and shady environment; retaining two nodes on each cutting, cutting ends 1.5-2 cm apart from the upper and lower nodes, removing leaves and tendrils on the lower nodes, retaining a compound leaf on the upper portion, and preventing leaves and stems from being broken during the operation; then fixing the cut cuttings in a container with a water matrix, arranging them in order, separating rows with partitions, and ensuring that the water matrix is ​​10 cm deep, keeping the lower nodes 2-3 cm in the water matrix, transplanting 1000 cuttings per square meter, and using a sunshade net with a 50% sunshade rate for sunshade. This method uses water as the matrix, has a high survival rate of cuttings, does not require other matrices such as peat, is easy to obtain, has little pollution, and has a propagation efficiency that is more than 5 times that of other methods. It significantly reduces labor costs and is conducive to the rapid propagation of long-stemmed Gynostemma pentaphyllum.

The invention discloses a fertilization method and system for improving CO2 absorption efficiency of greenhouse plants based on an SPAC system. The method comprises the following steps: acquiring moisture information and environment information of the greenhouse plants through a biological environment information acquisition module deployed in a greenhouse; according to the obtained moisture information and environment information, the current leaf water potential and the saturation vapor pressure difference are calculated; calculating the stomatal conductance of the plant by combining the soil water potential and the atmospheric pressure according to the calculated current leaf water potential and saturation vapor pressure difference; and regulating and controlling water and CO2 fertilization of the greenhouse plants according to the stomatal conductance of the plants. By means of the method, under the condition that the evaporation intensity is too high, plant water stress can be weakened, stomatal opening is promoted, stomatal conductivity and mesophyll conductivity are improved, CO2 absorption and utilization are promoted, the photosynthesis efficiency of greenhouse fruits and vegetables can be effectively improved, and plant growth and yield formation are promoted.

The application discloses a method for making ornamental plant leaves into skin-friendly artware after artificial posture setting treatment and a preparation method thereof, and comprises the following steps: 1) pretreatment, 2) enhancement treatment, 3) coloring, 4) leaf shaping, and 5) combination. The plant which is originally soft, not enough to stand upright and difficult to shape is reinforced by a preservative and polyethylene material, so that the thickness of the leaf vein is increased from 2 mm to 3 mm, and the treated plant leaves have gloss and high flexibility. The plant leaf artware is skin-friendly and environment-friendly, and is beneficial to resource reuse. The plant leaf artware has high practicability in the field of decoration and environment-friendly products.

The invention relates to the technical field of essential oil extraction, and discloses hydrolat extraction equipment for silphium perfoliatum, the hydrolat extraction equipment comprises an evaporation kettle, a condensation mechanism, a steam generation mechanism and a separation mechanism, a base layer sieve plate is arranged in the evaporation kettle, and a stirring mechanism is arranged on the evaporation kettle; the stirring mechanism comprises a driving part and a stirring part detachably connected with the driving part; a layering mechanism is arranged in the evaporation kettle. According to the extraction equipment provided by the invention, an ordered steam channel is constructed through a layered sieve plate structure, layered evaporation placement of petals and leaves can be realized, layered heating of the petals and the leaves can be ensured, and an ejector rod reciprocating lifting mechanism is utilized, so that on one hand, petal layer gaps are dynamically adjusted in the initial stage of evaporation to promote uniform permeation of steam; and on the other hand, the channel blockage is avoided by continuously cleaning materials adhered to the sieve pores, so that the stable penetrability of high-temperature steam in multiple layers of raw materials is guaranteed, and the volatile matter extraction efficiency is improved.

A new black locust tree ‘OBE TSZM’ particularly distinguished by elliptic leaves with an obtuse apex; bodkin like thorns; straight, pinnate growth habit; and blooms profusely and is a useful bee pasture, well-liked by beekeepers, is disclosed.

The application discloses application of a PHB9 gene in regulating rice leaf morphology and belongs to the technical field of genetic engineering.The CDS sequence of the PHB9 gene is shown as SEQ ID NO.2.It is found through experiments that overexpression of the PHB9 gene increases leaf length and leaf width, and the PHB9 gene has a significant effect on regulating rice leaf morphology.Further, changes in vascular systems, mitochondria and chloroplast structures in the internal structure of the leaf are analyzed, and it is shown that the vascular systems, mitochondria and chloroplast structures change when the leaf morphology is regulated, and the morphological changes in the internal structure of the leaf affect the changes in the leaf phenotype, and have a significant influence on improving the photosynthetic rate of the leaf.It is also found that overexpression of the PHB9 gene reduces the sensitivity of rice seeds to ABA, promotes seed germination and root growth.The application provides a new gene for breeding of leaf morphology building, root development and seed germination.

The invention discloses a blackberry RubHLH1 gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the blackberry RubHLH1 gene provided by the invention is as shown in SEQ ID NO. 1, and the amino acid sequence of expressed protein is as shown in SEQ ID NO. 2. The RubHLH1 gene is genetically transformed into arabidopsis thaliana, and a RubHLH transgenic arabidopsis thaliana plant shows a plurality of beneficial characters compared with a wild type plant at the same growth time, including that the stem length is fast and thick, the SPAD value is high, and podding is carried out in advance; wherein stem segments and petiole epithelium spines of the bHLH-3 strain are more than those of a wild type, the leaf surface is more shrunk, and the number of pores is large. The activities of POD, SOD and CAT and the contents of MDA, H2O2, total phenols and flavonoids in a transgenic plant are higher than those in a CK contrast. The invention provides an efficient gene resource and a molecular tool for cultivating excellent transgenic plant varieties by using a molecular breeding means.

The variety ‘LULU’ is a cultivar of the Dracaena deremensis plants. It can reach 66 cm height in a 21 cm diameter pot, with an average of approximately 40-46 leaves per plant. The arrangement of the leaves is a rosette disposition wrapped around the stem. Shows a dark green colored foliage in lower leaves, and a light green in the upper leaves.

The application relates to the technical field of molecular biology, and discloses a cold-tolerance identification method of Pennisetum based on a 65-core molecular marker set, a primer group and a gene chip and application thereof. The application collects core germplasm resources of the Pennisetum through a system, and constructs a GWAS population by using multi-environment (different altitudes) conditions, so that a wide genetic background and phenotype variation are fully covered, and a material foundation for genetic analysis of cold tolerance is laid. In the aspect of cold-tolerance phenotype identification, the application innovatively takes the chlorophyll content of leaves and the percentage of green leaves after natural low-temperature stress in autumn and winter fields as the cold-tolerance evaluation indexes, judges individuals with more than 40 excellent allelic variations as high-cold-tolerance varieties, and establishes a rapid and non-destructive phenotype screening method which can be implemented at the seedling stage.

The present invention provides a method for vegetative propagation of non viviparous tropical water lilies by dividing the living plants. From August to September every year, the selected non viviparous tropical water lilies are evenly divided into 2 - 4 pieces starting from the roots. Each divided plant ensures that it contains at least one healthy leaf bud point and several mature leaves. Then, after division, they are planted in a submerged potted manner, with one divided plant planted in each pot, keeping the leaf bud point level with the soil surface of the planting. In the early stage, the water level above the leaf bud point is maintained at 5 - 10 cm, and the water level is gradually increased by 15 - 40 cm in the later stage. After vegetative propagation by dividing the living plants according to the present invention, the non viviparous tropical water lily plants can quickly resume growth and can grow and bloom normally 1 - 2 months later. The divided plants retain the flower color and traits of the original plants, having high economic value and ornamental value. And the divided non viviparous tropical water lilies can be divided and propagated again under permitted environmental conditions, further improving the propagation rate and reducing the cost of seedling breeding.