59 results about "Morphological trait" patented technology

The invention discloses a bacillus strain capable of efficiently degrading DMP (dimethyl phthalate), a culture method and application thereof to remediation of soil PAEs (phthalic acid esters) pollution. A strain QD-9-10 which grows by mainly taking DMP as a carbon source is separated and identified from black soil covered with mulching films for a long term by adopting inorganic salt culture medium. According to morphological characteristic observation of a colony and 16SrDNA base sequence determination and homological analysis, the identification result of the bacterium is that QD-9-10 belongs to Bacillus subtilis. According to results of orthogonal experiments, the optimum culture method of the strain QD-9-10 is to perform culture by adopt inorganic salt culture medium under culture conditions that pH is 8.0, temperature is 35DEG C and shaking speed is 150rpm and DMP initial concentration is 100mg L<-1>, and biomass reaches the maximum at 32h. In addition, according to substrate utilization tests, the strain QD-9-10 can also utilize other common PAEs. Accordingly, the Bacillus subtilis QD-9-10 strain has a capability of degrading DMP and other common PAEs, and has a certain application prospect in aspects of degrading PAEs pollutants and remedying soil PAEs pollution.

Neural net method of computing oral cancer risk based on inputs such as age, gender, smoking status, morphological characteristics of sampled cells, and levels of biomarkers in samples cells.

The invention relates to a flotation dosing abnormity detection method based on NSST morphological characteristics and depth KELM. The method comprises the following steps: firstly, acquiring a bubble image on the surface of a flotation tank in real time, and decomposing the image NSST to obtain a low-frequency sub-band image and a multi-scale high-frequency sub-band; secondly, binarizing the low-frequency image to extract bubble bright spots, calculating the number, the area, the standard deviation and the ellipticity of the bright spots, and calculating the fractal dimension, the mean value and the variance of high-frequency sub-band coefficients of all scales to form multi-scale morphological characteristics of the bubble image; then, on the basis of the KELM algorithm, a deep KELM is constructed by referring to a deep learning idea, quantum calculation is introduced into optimization of a genetic algorithm and used for optimizing parameters of the deep KELM, and an adaptive deep KELM is constructed; and finally, establishing a flotation dosing abnormity detection model through the multi-scale morphological characteristics and the self-adaptive depth KELM. The average recognition rate and the operation efficiency are obviously higher than those of an existing detection method, the requirement for flotation production on-line detection is better met, and a foundation is laid for follow-up dosing automatic control.

The present invention relates to a method for calculating the body condition score BCS, the weight of an animal and its state of fertility by means of the mathematical processing of some characteristic morphological traits of the observed subject, which makes use of at least one contact or no-contact detection device of the profile 109 of the animal, at least a data processing unit and a program that implements a specific mathematical method of interpretation. By such a method, the determination of the body condition and its synthetic index or fattening index or Fl, is independent of species,race, gender, age and absolute size of the examined animal. This method is also robust to possible errors of positioning of the apparatus by an operator.

The invention discloses a novel species of actinomyces resisting heavy metal lead and application thereof. The bacterium species belongs to the streptomyces, is named as streptomyces plumbiresistens sp.nov, and is applied to the biological repair of an environment with heavy metal lead pollution. System research is carried out to a bacterial strain CCTCC M 209033 with higher lead-resisting characteristic by verification methods of morphological characteristics, cultural characteristics, chemical component analysis of cell walls, physiological-biochemical characteristics, 16S rDNA complete-sequence analysis, DNA-DNA hybridization, and the like, the similarity between the bacterial strain and the 16S rDNA of the known streptomycete is smaller than 99.0%, the DNA autoploidy is smaller than 50%, and a result indicates that the bacterium species belongs to a novel species of the streptomycete. The bacterium species is suitable for the biological repair of a polluted environment, particularly to an environment polluted by Pb.

The invention discloses a DNA barcoding standard detection sequence for acrossocheilus beijiangensis, which is shown as SEQ ID NO;1, and a molecular identification method based on the standard detection sequence. Due to the application of the DNA barcoding standard detection sequence for acrossocheilus beijiangensis provided by the invention, the reliable DNA molecular identification technology for the incomplete individuals which can not be accurately identified and for the complete individuals in samples is realized, therefore, the technical defect that in the prior art, the incomplete individuals basically can not be identified, and the complete individuals need to be identified by the professionals who are very familiar to the morphological traits of acrossocheilus beijiangensis is overcome, and compared with the traditional morphological identification method, the DNA barcoding standard detection sequence provided by the invention can be used for rapidly and accurately identifying acrossocheilus beijiangensis.

The invention discloses a molecular specific marker primer for identifying Chinese sturgeon, and a method. In the molecular specific marker primer for identifying the Chinese sturgeon, the upstream primer XYF is shown in SEQ ID NO.1; and the downstream primer XYR is shown in SEQ ID NO.2. The molecular specific marker primer XYF / XYR performs rapid molecular identification on the Chinese sturgeon bythe conventional PCR method, the method is simple, detection is conducted by a PCR technology, the time is short, detection can be completed in half a day, and the molecular specific marker primer and the method are effective assistance for morphological character discrimination on the Chinese sturgeon.

The invention discloses a counterpart river identification method based on morphological characteristics, in particular comprising the following steps: firstly, calculating the river flow direction based on a vector water system map of a research area and combining DEM data of the research area; and secondly, calculating the river flow direction based on the vector water system map of the researcharea. Secondly, the two straight river sections which constitute the river intersection angle are extracted and the river intersection angle is calculated. At last, two rivers with flat angle are recorded, and the corresponding river map layer is generated. The invention can quickly realize automatic extraction of the estuary river by calculating the flow direction, extracting the straight line edge, calculating the intersection angle of the rivers and other links on the existing vector water system network diagram. Compared with the prior art, the present invention not only improves the identification efficiency, but also effectively avoids misjudgment and missed judgment in manual operation.

The invention discloses a breeding method for wheat having fast grain filling and fast dehydration, and belongs to the field of wheat breeding. The method comprises the following steps: (1) carrying out parent research, identifying filling and dehydration characteristics of wheat varieties, and determining a parent cross combination; (2) according to seed selection requirements, determining a cross breeding or backcross breeding scheme; (3) in the seed selection process, comprehensively applying morphological trait investigation, studies on wheat grain filling and dehydration characteristics, different-stage sowing and yield identification, screening a genotype having fast grain filling, fast dehydration and high yield; and (4) breeding the new wheat variety having fast grain filling and fast dehydration. According to the breeding method for the wheat having fast grain filling and fast dehydration, the early generation morphological selection and the study on the grain filling characteristic and the dehydration characteristic are applied to wheat breeding at the same time, and the wheat material having fast grain filling and large dehydration rate can be accurately selected. The variety bred by the method has the advantages of early maturation, good maturation performance and grain plumpness. The filling rate is fast, the filling completing time is short and the thousand seed weight is high. The fast dehydration stage is immediately performed after high-efficiency completion of filling, the dehydration rate is fast, and the maturation period is short. Under the condition of late sowing, the wheat can be still harvested in advance, and crop rotation for rice can be performed in advance. The wheat adapts to new requirements of the current new wheat situation, and a foundation is laid for annual yield increase of rice and wheat in the middle and lower reaches of the Yangtze river.

The invention provides a method for studying growth of cephalopods through classification of beak pigmentation. The method comprises the following steps: grade classification is performed on beak pigmentation according to different positions and different degrees of beak pigmentation of Ommastrephidae and in combination of beak individual size and Ommastrephidae individual size; on the basis of the AIC (Akaike information criterion), relationships of pigmentation grades with biology and beak parameters are established, growth characteristics of the Ommastrephidae are studied, and growth stages of Ommastrephidae individuals are determined according to beak pigmentation grades. According to the method, morphological characteristics of cephalopod beaks are summarized, and laws of pigmentation in different periods and different positions are found out; the beak pigmentation grades of the cephalopods are classified rapidly and concisely; the relationships of the pigmentation grades with biology and the beak parameters are established for the first time, deep study are performed better on growth and ingestion conditions of the cephalopods, the pigmentation grades are in one-to-one correspondence with individual growth stages directly, and the growth stages of the individuals can be judged directly through the beak pigmentation grades.

The invention relates to a molecular specificity labeled primer for identifying a T. chinensis seedling and a method. An upstream primer NHSF of the molecular specificity labeled primer for identifying T. chinensis and two similar species including T. madia and T. cuspidata is represented as SEQ ID NO.1, and downstream NHSR is represented as SEQ ID NO.2; and by means of the molecular specificity labeled primer MHSF / MHSR, the T. chinensis and the two similar species of the T. chinensis can be rapidly subjected to molecular identification with a conventional PCR (polymerase chain reaction) method. The method is simple, the PCR technology is adopted for detection, time is short, and the detection can be finished by half day; and the molecular specificity labeled primer and the method provide effective assistance for identification of the T. chinensis and the two similar species of the T. chinensis according to morphological characteristics.

The invention discloses a waterlogging-resistant hemerocallis variety screening method. Waterlogging treatment is carried out on hemerocallis of different varieties, water is 5 cm higher than the soilsurface, submergence treatment continues for 6 days, and morphological characteristics, such as leaf color, leaf morphology, color changes of submerged stems and morphological changes 35-40 days after plant recovery, of the overground portions of plants are observed and recorded; by means of photographing recording, the grading ranges of the four indexes including the leaf color, leaf morphology,the color changes of the submerged stems and the morphological changes 35-40 days after plant recovery are determined first; after total points are obtained according to grading scoring standards, good-quality varieties high in waterlogging resistance are screened out. It is proved that the system can obtain reliable results, the true situation of the hemerocallis can be objectively reflected, and a foundation is laid for hemerocallis waterlogging-resistant breeding and large-scale hemerocallis variety screening.

The invention discloses a method and device for constructing Cynodon dactylon core germplasms with morphological markers. The method comprises the following steps: using original germplasms of Cynodondactylon as test materials, detecting 11 morphological traits through morphological marking, and selecting the best strategy to construct Cynodon dactylon core germplasms from 5 levels, including 7 kinds of total sampling proportion, 3 kinds of sampling methods, 5 kinds of clustering methods, 3 kinds of genetic distance, and 3 kinds of in-group sampling proportion. According to the embodiments ofthe invention, the constructed Cynodon dactylon core germplasms can effectively retain the phenotype genetic diversity, phenotypic trait correlation and population phenotype structure of the originalgermplasms and can be used as representative samples of a whole resource.

The invention discloses a molecular specific primer and a method for identifying catfish. The molecular specific primer for identifying catfish has a forward primer NYF shown as SEQ ID NO: 1, and a reverse primer NYR shown as SEQ ID NO: 2. By utilizing the molecular specific labeled primer NYF / NYR, catfish can be subjected to rapid molecular identification by a conventional PCR method. The methodis simple, is capable of finishing detection with PCR technology and is an effective assistance for identifying catfish through morphological characteristics.

The invention discloses an intelligent cell identification method based on the laser confocal microscopy technology. The method comprises the following steps: (1) carrying out culture and fluorescentstaining treatment on cells; (2) adopting a laser confocal microscope to obtain a layer-cut image of a cell multi-fluorescence channel; (3) sequentially carrying out graying, denoising processing andthreshold segmentation on the layer-cut image, picking out a cell region, and storing gray data of the same fluorescence channel into a three-dimensional matrix; (4) determining each cell region and labeling by adopting a region random growth model; (5) sequentially carrying out interpolation three-dimensional reconstruction on the labeled cells, and calculating three-dimensional morphological parameters of each cell; (6) repeating the processes (1) to (5) by using different cells, and learning the three-dimensional morphological characteristics of the cells by using a machine learning methodto finally realize the identification of various cells. According to the identification method provided by the invention, various cells can be rapidly and effectively identified on the basis of the three-dimensional morphological characteristics of the cells obtained by the laser confocal microscopy technology.

The present invention discloses standard Lentinus edodes seed detecting method, which includes 5 tested aspects including morphological characteristic, agronomic character, physiological character, quality character and molecular fingerprint of special DNA segment, and 36 test indexes, including 34 essential ones and 2 optional ones. Of the 34 essential indexes, there are 10 hypha character indexes and 24 cultivating character indexes.

The invention provides a DNA bar code for discriminating aegilops tauschii and aegilops cylindrica and an application thereof. A nucleotide sequence of DNA bar code is shown in as SEQ ID NO: 1-2. By designing a pair of novel DNA bar code primers, an optimal identification sequence is obtained, the DNA bar code sequences of aegilops tauschii and aegilops cylindrica in a Genbank database are improved, aegilops tauschii, aegilops cylindrica and aegilops umbellulata can be distinguished by only one pair of the primers, and the unknown aegilops tauschii and aegilops cylindrica can be rapidly identified. Compared with a form characteristic identification method at a growth anaphase, the DNA bar code breaks up the restriction of an identification period, greatly increases the identification efficiency, increases the detection speed and accuracy, is in favor of guidance for timely controlling two wheat field worst weeds for an agriculture production mechanism and peasants, ensures the production safety of wheat and peasant income, reduces the threat to national food security, and has good economic benefit and social benefit.

The invention discloses a modified graphene-brass composite material. The modified graphene-brass composite material is characterized by being prepared from the following materials: 5-15% of grapheneand 1-5% of humic acid, with the balance being brass. The modified graphene-brass composite material of the invention adopts graphene and humic acid as complementary additives and utilizes the uniquemorphological and structural characteristic of humic acid, and through mutual fitting between humic acid and graphene, the dispersibility of graphene is promoted without destroying the surface morphological structure of graphene; at the same time, humic acid has structural and morphological traits similar to the structural and morphological traits of graphene, so humic acid can be auxiliarily usedas a reinforcing phase for reinforcing the performance of the composite material.

The invention relates to a method for drawing morphology of fish eggs and larvae. The method performs developmental stage determination and preliminary species identification on the fish eggs and larvae based on a biological anatomical lens. The morphological characteristics of the fish eggs and larvae are subjected to shooting processing by a high-resolution microscopy, then pictures of various parts of the fish eggs and larvae are taken and Photoshop software is used for picture overlay processing, and finally the pictures with all the clear morphological characteristics of the fish eggs andlarvae are exported; and then the photographed fish eggs and larvae are subjected to molecular biological treatment to determine the species, then the pictures are matched with the species, the pictures are copied and processed on vegetable parchment by printing the pictures and placed into a scanner for scan processing, and finally the morphological characteristics and morphological developmentpatterns of the fish eggs and larvae are obtained. The method overcomes the deficiency that the morphological characteristics of the fish eggs and larvae need to be drawn by experts engaged in drawingand the related fields of fish features in the past, and has the advantages of being fast, accurate and efficient.

The invention discloses a method for three-dimensional reconstruction of a plant population morphological structure, relating to the technical field of three-dimensional graph generation. The method comprises the following steps of: S1, measuring canopy morphological character parameter data of a plant population to be reconstructed; S2, constructing a plant population canopy morphological character statistical model according to the morphological character parameter data; S3, acquiring position information and internode number information of each plant of the plant population to be reconstructed; S4, reconstructing a three-dimensional model of each plant in the plant population to be reconstructed according to the internode number information acquired in the step S3 and the canopy morphological character statistical model constructed in the step S2; and S5, according to the position information, moving the three-dimensional model of each plant to a corresponding position of a three-dimensional scene to finish the three-dimensional reconstruction of the plant population to be reconstructed. By the method, the three-dimensional reconstruction of the plant population can be accurately and simply performed.

The invention discloses a rapid sex identification method for conopomorpha sinensis. The method comprises the following steps: collecting a pupa or an adult of conopomorpha sinensis: when the back surface of the third section of the conopomorpha sinensis pupa is yellow, the seventh section of the ventral surface is provided with a reproductive hole, the maxillary palp of the adult is sturdy and / or the ventral surface of the tail section of the abdomen is longitudinally cracked, the conopomorpha sinensis is male; and when the back surface of the third section of the conopomorpha sinensis pupa is not yellow, the seventh section of the ventral surface has no reproductive hole, the maxillary palp of the adult is thin and / or the ventral surface of the tail section of the abdomen has no cracking and end truncation, the conopomorpha sinensis is female. According to the method, the cost is hardly needed, the operation is very simple, and related researchers can completely master the method only by being slightly familiar with the four morphological characteristics.

The invention discloses a deoxyribonucleic acid (DNA) sequence of a narrow-headed vole. The invention aims to provide a method for quickly identifying the narrow-headed vole, wherein the rat species are identified by a morphological characteristic identification method, and COI gene sequences are obtained. The invention also provides a DNA standard gene, a primer and a kit for detecting the narrow-headed vole. The narrow-headed vole is identified by adopting the traditional morphological identification combined with a molecular classification method, so that the accuracy and the reliability of species identification can be ensured.

The invention discloses an antarctic subglacial water detection method based on morphological characteristics of base signals. The method comprises the following steps: 1, preparing radio echo sounding data; 2, constructing a standard for describing the horizontal morphological characteristics of the substrate signal: 2.1, acquiring elevation data of an ice bed, 2.2, obtaining three indexes capable of describing horizontal morphological characteristics; and 2.3, constructing a standard, specifically, in order to highlight the position with strong elevation horizontal morphological characteristics of the ice bed, defining the standard for describing the elevation horizontal morphological characteristics of the ice bed in the specification; 3, constructing a standard for describing the vertical morphological characteristics of the substrate signal: 3.1, acquiring base signal data, 3.2, obtaining five indexes capable of describing vertical morphological characteristics: (1) responsivity, (2) degree of symmetry, (3) thin height, (4) thinness, and (5) the deviation degree of the substrate interface, and 3.3, constructing a standard; and 4, obtaining the possibility that the substrate has water under ice: 4.1, conducting preliminary possibility judgment, and 4.2, conducting final possibility judgment and classified display.

The invention provides a reproduction updating method for soybean heredity integrity analysis. The reproduction updating method includes: performing artificial aging treatment on parent seeds, selecting parent treatments at different germination rate gradients, raising the treated seeds, and transplanting the seedlings to a field; harvesting single plants; and performing seedling raising, transplanting, morphological trait investigation, and single plant harvesting on each descendant treatment according to the step of parent treatments. One of keys of the reproduction updating method is the fact that descendant single plants are corresponding to the parent single plants thereof in a one-to-one manner, i.e., the number of each single plant of each descendant treatment is corresponding to that of each parent single plant in a one-to-one manner; morphological marker analysis and SSR molecular marker analysis is performed during reproduction and updating of a parent generation and a descendant generation so as to detect the soybean heredity integrity. The reproduction updating method is complete and feasible, is normative in system, can reproduce and update stored high-viability soybean so as to maintain the heredity integrity of the soybean.

The invention discloses an engraving specimen and manufacturing method thereof. The engraving specimen includes a substrate made of transparent material, and a morphological characteristic enlarged drawing of the specimen is engraved on the substrate. The engraving specimen provided by the invention can be directly observed without using expensive equipment such as a high-power microscope; an organic material is beautiful and transparent, thereby facilitating viewing and appreciating; organic glass or resin is convenient for long-time storage, does not need a special storage environment and is not easy to damage; important morphological identification characteristics can be drawn according to identification requirements; and the engraving specimen is convenient to prepare and low in cost. The engraving specimen is low in preparation and identification cost and high in usage rate, is convenient for storage, the specimen preparation and storage are simplified, and the usage quality and efficiency of plant pathogenic nematode and pathogenic fungus specimens in scientific research and teaching, biological science popularization, exemplary education and quarantine identification are improved.

The invention discloses a tobacco seed-stage morphological classification characterization method based on sample consensus. The method is characterized by comprising the following steps: (1), selecting 100 tobacco plants, consecutively numbering and hanging plates, selecting morphological classification characterization samples at different seed stages from the plate-hung tobacco plants; (2), combining with the conventional classification of the tobacco seed stage, roughly characterizing; (3), quantitative classification characterization: performing the quantitative classification characterization according to different ratios of buds, flowers and fruits of the tobacco plants, classifying the tobacco seed-stage into 6 phases; and (4), sampling the tobacco plant samples at different phases from the tobacco plants within the quantitative classification range suitable for the phases, so as to meet the requirements of sample consensus. Compared with the existing classification method, the method adopts different ratios of visual buds, flowers and fruits, quantizes the objective morphological characterization of the tobacco seed stage, can accurately classify in standard, be easily operated, and beneficial to collect uniform tobacco seed-stage samples, so as to better analyze and determine the biological information of the samples, and more accurately reflect the actual information of the samples.

The invention provides a detection and identification method for an acanthocephalan worm of neoechinorhynchus. The method comprises the following steps that according to morphological characteristics,the acanthocephalan worm of neoechinorhynchus in a fish host is collected; DNA of a worm body sample is extracted; agarose gel electrophoresis is carried out to detect the quality of the extracted DNA, if the quality is qualified, a reaction system is established, and PCR amplification is carried out; and a sequencing result is subjected to sequence alignment, and if the consistency is 99 % or above, it is judged that the species is the acanthocephalan worm of neoechinorhynchus. The identification method has the advantage that the experimental results are accurate and reliable.