A Bacillus strain capable of efficiently degrading dmp, its cultivation method and its application in remediating soil paes pollution

A technology of Bacillus sp. and Bacillus subtilis, applied in the field of microorganisms, can solve problems such as affecting the function of black soil ecosystem, threatening black soil soil fertility and food production, changing black soil respiration and enzymatic activity and metabolic characteristics, etc.

Active Publication Date: 2018-06-05
QIQIHAR UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some research results show that DMP pollution can change the metabolic characteristics of black soil respiration and enzyme activity, affect the ecosystem function of black soil, and then threaten the soil fertility and food production of black soil

Method used

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  • A Bacillus strain capable of efficiently degrading dmp, its cultivation method and its application in remediating soil paes pollution
  • A Bacillus strain capable of efficiently degrading dmp, its cultivation method and its application in remediating soil paes pollution
  • A Bacillus strain capable of efficiently degrading dmp, its cultivation method and its application in remediating soil paes pollution

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1 Isolation of bacterial strain QD-9-10 and its application in degrading PAEs

[0024] 1 Materials and methods

[0025] 1.1 Test materials and medium

[0026] Dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), di-n-octyl phthalate (DOP), di-phthalate Isooctyl ester (DEHP), and diisooctyl phthalate (DIOP), methanol, ethyl acetate, and hexane, all chemicals and solvents were analytical reagents, purchased from Guangfu Institute of Fine Chemicals, Tianjin.

[0027] Basic mineral salt (MSM) medium: K 2 HPO 4 12H 2 O 1.0g, KH 2 PO 4 1.0g, NH 4 Cl 0.8g, NaCl 1.0g, MgSO 4 ·7H 2 O 0.5g, CaCl 2 0.006g, FeSO 4 ·7H 2 O 0.02g, trace element stock solution (MnSO 4 ·H 2 O0.1g, ZnSO 4 ·7H 2 O 0.12g, H 3 BO 3 0.07g, Na 2 MoO 4 ·H 2 O 0.04g, CuSO 4 ·5H 2 O 0.02g, CoCl 2 0.04g, distilled water to make up to 1000ml) 2mL, distilled water to make up to 1000ml, pH 7.0.

[0028] LB medium: peptone 10.0g, yeast powder 5.0g, sodium ch...

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Abstract

The invention discloses a Bacillus strain capable of efficiently degrading DMP, its cultivation method and its application in remediating soil PAEs pollution. The invention adopts inorganic salt culture medium to isolate and identify a strain from black soil covered with agricultural film for a long time Strains that primarily grow on DMP as a carbon source: QD‑9‑10. According to the observation of the morphological characteristics of the colonies and the determination of the 16S rDNA base sequence and homology analysis, the identification result of the bacteria was: QD‑9‑10 belonged to Bacilus subtilis. Orthogonal experiment results show that the optimal culture method of strain QD‑9‑10 is: culture with inorganic salt medium, culture conditions are: pH 8.0, temperature 35°C, shaker speed 150rpm, initial concentration of DMP 100mg L‑1 , the biomass reached the maximum at 32h. Meanwhile, the substrate utilization test showed that strain QD‑9‑10 could also utilize other common PAEs. It can be seen that the Bacilus subtilis QD‑9‑10 strain has the ability to degrade DMP and other common PAEs, and has certain application prospects in degrading PAEs pollutants and remediating soil PAEs pollution.

Description

technical field [0001] The invention relates to a PAEs degrading bacterium and its use, in particular to a Bacillus strain capable of efficiently degrading DMP and its use in degrading PAEs and remediating soil PAEs pollution. The invention belongs to the technical field of microorganisms. Background technique [0002] Phthalates (PAEs) are organic compounds widely used in plastic processing and many other industrial consumer products. The widespread use of phthalates has resulted in more and more phthalates entering our ecosystem. The environmental and human health problems caused by PAEs pollution have aroused widespread concern. Due to the carcinogenic, teratogenic and mutagenic effects of PAEs (Song Jiaoyan, Liu Dajun, Wan Yang, et al. Study on kinetics and infrared spectrum characteristics of humic acid adsorption of dibutyl phthalate[J]. Southwest University Journal (Nature Science Edition), 2014,3:022.), the U.S. Environmental Protection Agency has listed 6 PAEs as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/125A62D101/28
CPCA62D3/02A62D2101/28B09C1/10C12N1/20C12N1/205C12R2001/125
Inventor 王志刚徐伟慧莫继先朴依彤王洋董晓楠胡云龙胡影刘帅王春龙
Owner QIQIHAR UNIVERSITY
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