Pit mud microorganism separation method

Abstract

The preset invention belongs to the technical field of microorganism separation and discloses a pit mud microorganism separation method. The pit mud microorganism separation method comprises the following steps: step 1, collecting pit mud by a five-point sampling method; step 2, weighing 10 g of a pit mud sample to prepare a pit mud suspension; step 3, after water bath, inoculating the pit mud suspension into an enrichment culture medium, coating the enrichment culture medium into a separation culture medium by adopting a gradient dilution manner, placing the inoculated separation cultured medium into an anaerobic tank, performing separation culture, and performing plate streaking purification on microorganism colonies with various different characteristics to obtain single microorganism colonies; and step 4, streaking and separating the obtained single microorganism colonies and conducting caproic acid bacteria identification of the separated strain. Vinasse immersion liquid is used as the culture medium to simulate primary growth environment of microorganisms, so that the microorganisms in the pit mud can rapidly adapt to the new environment, activation time of the microorganismsis shortened, and microorganism separation efficiency can be improved.

Description

technical field [0001] The invention belongs to the technical field of microorganism separation, and in particular relates to a method for separating microorganisms from pit mud. Background technique [0002] To produce Luzhou-flavor wine, pit mud is the foundation, Daqu is the driving force, and craftsmanship is the key. To make Luzhou-flavored high-quality wine, the quality of pit mud must first be grasped. The quality of pit mud directly determines the quality of wine. Because pit mud is the carrier and habitat of caproic acid bacteria, methane bacteria, butyric acid bacteria and other beneficial substances, as well as its breeding ground, the type and quantity of these beneficial microorganisms are a standard to measure the quality of pit mud. The main aroma substance of Luzhou-flavor Daqu liquor is ethyl caproate, and ethyl caproate is a metabolite of various aroma-producing and ester-producing microorganisms such as Clostridium (caproic acid bacteria) in the pit mud,...

AI Technical Summary

Problems solved by technology

At present, the method of heating in a water bath at 80°C is usually used to pretreat the pit mud samples, because the spores of caproic acid bacteria are heat-resistant, while their vegetative cells are not heat-resistant, weaker spores can be eliminated by heating, and at the same time Kill vegetative cells and other miscellaneous bacteria, but there are many kinds of microorganisms in the pit mud, and there are many types of heat-resistant spores. It is difficult to screen out other miscellaneous bacteria by only using water bath heating method to screen out other miscellaneous bacteria, resulting in the separation of bacteria The purification process requires separation and identification of many samples, which is cumbersome and inefficient

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