12 results about "Bacteria identification" patented technology

The invention discloses a bacterium capable of resisting serious combined pollution of arsenic and cadmium. The bacillus cereus strain is identified as bacillus cereus, is named as bacillus cereus L1. GLUT, and is preserved in the China General Microbiological Culture Collection Center on April 10, 2024, the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.30292. The bacillus cereus strain is named as bacillus cereus L1. GLUT. The bacillus cereus L1. GLUT can resist 200 mg / L of As and 100 mg / L of Cd at the same time, can be prepared into a microbial agent to be combined with a hyperaccumulator, and has considerable application value in the aspect of remediation of arsenic and cadmium combined contaminated soil.

The application discloses a kind of nucleic acid aptamer specifically binding meso-diaminopimelic acid and application thereof, belong to the field of bioengineering and detection technology.The sequence of the nucleic acid aptamer is as shown in SEQ ID NO:2, or variant obtained by the addition, deletion or substitution of one or more bases and has the same binding function, and is obtained by screening by an improved Capture-SELEX method, which introduces progressive counter-screening strategy in screening process, effectively improves the specificity of aptamer.The preferred aptamer 34S (SEQ ID NO:3) provided by the application is verified by isothermal titration calorimetry, and has nanomolar level affinity with meso-diaminopimelic acid (DAP).The aptamer can be used as a core recognition element to construct electrochemical, optical and other types of biosensors, detection kits, rapid detection systems or equipment, to realize rapid, sensitive and specific detection of DAP, and has important application value in the field of bacterial identification, infectious disease identification and the like.

Provided are antibacterial phages that selectively kill bacteria having a resistance gene or the like. Antibacterial phage for this includes CRISPR-Cas13a with a target sequence that recognizes a specific gene as a target. This target sequence is designed as a spacer sequence for crRNA of 14-28 bases. Specific genes are drug resistance genes and toxins. The drug resistance genes are included in bacterial genomes and / or plasmids having one or any combination of the group including: methicillin-resistant Staphylococcus aureus, vancomycin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, penicillin-resistant pneumococcus, multidrug-resistant Pseudomonas aeruginosa, multidrug-resistant Pseudomonas aeruginosa, carbapenem-resistant Pseudomonas aeruginosa, carbapenem-resistant cephalosporins, third-generation cephalosporin-resistant Pseudomonas aeruginosa, third-generation cephalosporin-resistant E. coli, and fluoroquinolone-resistant E. coli.

The utility model discloses a full-automatic omnibearing bacteria identification and drug sensitivity tester, which comprises a microorganism drug sensitivity analyzer and a bearing block, the bearing block is fixedly connected with a damping block, the lower surface of the microorganism drug sensitivity analyzer is fixedly connected with a connecting block, and the inner surface of the bearing block is fixedly connected with a plurality of bearing rods. Through the arrangement of rolling wheels, telescopic air cylinders, anti-skid pads and other structures, when the whole equipment needs to be moved into a room, fixing of the equipment is relieved firstly, then the equipment is pushed, and therefore under the action of the rolling wheels, the whole equipment can be moved, a manual carrying mode does not need to be adopted any more, the labor intensity of workers is relieved, and waste of human resources is reduced; and after the equipment moves to a proper position, a connecting plate, a fixing block and an anti-skid pad make contact with the ground by starting a telescopic air cylinder, then the position of the device can be fixed, and under the action of the anti-skid pad, the stability of the device after fixing can be improved.

The invention discloses a nucleic acid aptamer specifically combined with meso-diaminopimelic acid and application of the nucleic acid aptamer, and belongs to the technical field of bioengineering and detection. The sequence of the nucleic acid aptamer is shown as SEQ ID NO: 2, or a variant which is obtained by adding, deleting or replacing one or more basic groups on the sequence and has the same binding function, the nucleic acid aptamer is obtained by screening through an improved Capture-SELEX method, a progressive reverse screening strategy is introduced in the screening process, and the specificity of the aptamer is effectively improved. The optimal aptamer 34S (SEQ ID NO: 3) provided by the invention is verified by an isothermal titration calorimetry, and the optimal aptamer 34S (SEQ ID NO: 3) and meso-diaminopimelic acid (DAP) have nano-mole-level affinity. The aptamer can be used as a core recognition element, is used for constructing electrochemical, optical and other types of biosensors, detection kits, rapid detection systems or equipment, realizes rapid, sensitive and specific detection of DAP, and has important application value in the fields of bacterial identification, infectious disease recognition and the like.

Devices for performing growth detection, identification and antimicrobial susceptibility testing (AST) functions in connection with a microbial species to achieve sample-to-answer results are disclosed. Methods of performing microbial growth, microbial species identification, and AST using such devices are also disclosed.

The invention relates to the technical field of bacterium identification instruments, in particular to a portable bacterium identification instrument and a using method thereof, and the portable bacterium identification instrument comprises a shell, a flip cover, a detection circuit board, a rotating disc, a mounting column, a rotating column and a detection assembly; the shell is in a cuboid shape, the interior of the shell is of a hollow structure, the flip cover is arranged on the upper face of the shell, the detection circuit board is installed in the shell, the rotating disc is installed beside the detection circuit board and can rotate around the center point of the rotating disc, and the installation column is installed at the center of the upper face of the rotating disc and can rotate around the center point of the rotating disc. The rotating column is mounted on the outer side of the mounting column, and the detection assembly is mounted on the mounting column; the detection assembly is driven by the rotating disc to rotate, and the generated centrifugal force can transport the detection liquid of the detection bottom plate into the storage cavity, so that the cleaning work is completed.

The invention discloses a method and a device for separating and culturing microorganisms from a large number of liquid samples, and the method comprises the following steps of: pouring or dropwise adding a quantitative liquid sample to be detected onto the surface of an agar culture medium by using a traditional agar culture medium by using a sterile method; a dry powder spraying instrument designed by the invention is used for spraying sterile polymer dry powder onto an agar culture medium, the polymer is cross-linked to form a three-dimensional structure adsorption layer, microorganisms in a sample are rapidly fixed on the surface of the agar culture medium, the microorganisms of a curing layer are incubated and cultured to form bacterial colonies for detection, and the bacterial colonies can be counted; the bacterial colonies can also be directly applied to further operations such as bacterial identification, drug sensitive test or other microbiological analysis and detection. According to the method, on the basis of a traditional solid culture scheme, a large number of liquid samples are directly subjected to solid treatment, integration of the samples and the agar culture medium is achieved, and the large number of liquid samples can be cultured on the agar culture medium.

Provided are antibacterial phages that selectively kill bacteria having a drug resistance gene or the like. Antibacterial phage for this includes CRISPR-Cas13a with a target sequence that recognizes a specific gene as a target. This target sequence is designed as a spacer sequence for crRNA of 14-28 bases. Specific genes are drug resistance genes and toxins. The drug resistance genes are included in bacterial genomes and / or plasmids having one or any combination of the group including: methicillin-resistant Staphylococcus aureus, vancomycin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, penicillin-resistant pneumococcus, multidrug-resistant Pseudomonas aeruginosa, multidrug-resistant Pseudomonas aeruginosa, carbapenem-resistant Pseudomonas aeruginosa, carbapenem-resistant cephalosporins, third-generation cephalosporin-resistant Pseudomonas aeruginosa, third-generation cephalosporin-resistant E. coli, and fluoroquinolone-resistant E. coli.

The application belongs to the technical field of bacteria identification, and discloses a multi-dimensional bacteria spectrum acquisition method, a bacteria identification method and an application device, wherein the multi-dimensional bacteria spectrum acquisition method comprises the following steps: cracking a bacteria suspension by using ultrasonic waves to obtain a bacteria lysate, mixing a plurality of surface-enhanced Raman scattering concentrated suspension substrates with the bacteria lysate respectively to obtain a plurality of mixed liquids, forming a liquid film by using the plurality of mixed liquids, performing surface-enhanced Raman scattering analysis on the liquid film, and obtaining multi-dimensional surface-enhanced Raman scattering bacteria spectrum data. The application can acquire multi-dimensional spectrum information of bacteria, and then realizes rapid and accurate detection and identification of bacteria samples.

The application discloses a kind of bacterial identification microfluidic chip and use method, including bacterial identification reagent disc, with the center of bacterial identification reagent disc as center, from center to the outside of bacterial identification reagent disc, it is sequentially provided with shaft hole, bacteria liquid sample adding groove, annular flow guide flow channel;Bacteria liquid sample adding groove is arranged outside shaft hole;Annular flow guide flow channel is arranged outside bacteria liquid sample adding groove, and bacteria liquid sample adding groove and annular flow guide flow channel are connected by bending type microfluidic channel;Bacterial identification reaction cavity is arranged outside annular flow guide flow channel, and bacterial identification reaction cavity is communicated with annular flow guide flow channel by bending type microfluidic channel.By the above structural design, the application realizes in improving the efficiency of bacterial identification, while guaranteeing for bacterial identification process in no pollution, identification accuracy, meet the demand of bacterial biochemical property change in bacterial identification process, and the bacterial identification microfluidic chip high applicability, it is easy to operate.

The invention discloses a standardization method and system for pathogen metagenome background bacterium level identification, and relates to the technical field of clinical pathogenic microorganism detection, the standardization method comprises the following steps: screening core background flora based on metagenome sequencing data; constructing a historical reference library; screening a background bacterium marker; evaluating background bacterium signal intensity; and determining the background bacterium level. According to the standardization method and the judgment system for pathogen metagenome background bacterium level identification, clinical specimen types are not distinguished, a background bacterium judgment method based on the standardized pg amount is introduced, and it is ensured that background bacterium detection amounts of different samples have comparability; and meanwhile, the accuracy of a detection result and the reliability of clinical interpretation can be improved by utilizing a background bacterium identification mechanism with dual-index collaboration, a historical reference library and a dynamic updating mechanism.