23 results about "Bacteria identification" patented technology

The invention discloses a bacterium capable of resisting serious combined pollution of arsenic and cadmium. The bacillus cereus strain is identified as bacillus cereus, is named as bacillus cereus L1. GLUT, and is preserved in the China General Microbiological Culture Collection Center on April 10, 2024, the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.30292. The bacillus cereus strain is named as bacillus cereus L1. GLUT. The bacillus cereus L1. GLUT can resist 200 mg / L of As and 100 mg / L of Cd at the same time, can be prepared into a microbial agent to be combined with a hyperaccumulator, and has considerable application value in the aspect of remediation of arsenic and cadmium combined contaminated soil.

The invention provides a primer group for identifying multiple genes of ten bacteria related to lower respiratory tract infection and application of the primer group, and belongs to the technical field of gene detection, the sequence of the primer group is shown as SEQ ID NO.1-20, the primer group designs specific primers for ten bacteria such as pseudomonas aeruginosa and streptococcus pyogenes, and the specific primers of ten genes of a 5'end primer are labeled by FAM fluorescence. 10 target gene loci and 2 internal reference loci can be detected in one PCR system, the traditional PCR deviation is overcome, and gene expression is accurately quantified. The reagent and the kit developed on the basis have the advantages of simplicity and convenience in operation, low cost, high accuracy and the like. When the kit is used, DNA of a sample is extracted for multiple PCR, and pathogen load is determined through electrophoresis and software analysis. Experiments prove that the method has strong specificity and high sensitivity to 10 bacteria, and has important application value in LRTI bacterial identification.

The invention provides a method for portable mass spectrum identification of bacterial species based on substrate-enzyme reaction, which comprises the following steps: culturing to-be-detected bacteria to obtain a bacterial culture solution, washing and diluting to obtain a bacterial suspension; adding a plurality of pre-selected substrates into the bacterial suspension for co-incubation, so that the bacterial suspension and the pre-selected substrates are subjected to enzyme reaction to obtain a bacterial substrate mixed solution; detecting the bacterial substrate mixed solution by using electrospray ionization mass spectrometry to obtain the mass spectrum signal intensity of the preselected substrates and the enzyme reaction product so as to calculate the conversion degree data of each preselected substrate; in a coordinate system based on a preselected substrate type and a transformation degree, drawing a characteristic column map of the to-be-detected bacteria; and matching the characteristic column spectrum with the characteristic column spectrum in the bacterium variety database, and identifying the variety of the to-be-detected bacterium. The method disclosed by the invention can realize species identification of bacteria in the bacterial infection biological sample within 30 minutes, has the advantages of rapidness, accuracy and wide application range, and can be suitable for field detection of the bacterial sample.

Disclosed is a device for performing growth detection, identification, and antimicrobial susceptibility testing (AST) functions for microbial species to achieve a sample-to-answer result. Methods for performing microbial growth, microbial species identification, and AST using such a device are also disclosed. The device may include a housing configured to receive a sample plate, a sample port configured to receive a sample, a fluid distribution system constructed and arranged to introduce the sample into one or more sample wells of the sample plate, a sample plate imaging system, and a controller configured to collect data from the sample plate imaging system. The controller may detect growth of the microbial species, identify the microbial species, and perform AST on the microbial species once the data is collected.

The application discloses a bacteria identification device and method based on volatile substance detection. The device comprises a high-purity gas cylinder, a flow controller, an air pipe, a volatile substance extraction unit, a detection unit and an industrial computer unit. Firstly, a characteristic library of bacterial volatile substances is established. Specifically, after bacterial standard samples are cultured, the volatile substances released by the bacteria are extracted by the volatile substance extraction unit and enter the detection unit under the driving of high-purity gas to obtain characteristic volatile substances of each kind of bacteria and establish the characteristic library. Then, the volatile substance information of bacteria in a biological sample is obtained by using the same extraction and detection method. Finally, identification is realized by comparing the algorithm with the characteristic library on the industrial computer unit. The application provides a brand-new device and method for bacterial identification, has the advantages of rapidness, high sensitivity and the like and has a good application prospect.

The application discloses a kind of nucleic acid aptamer specifically binding meso-diaminopimelic acid and application thereof, belong to the field of bioengineering and detection technology.The sequence of the nucleic acid aptamer is as shown in SEQ ID NO:2, or variant obtained by the addition, deletion or substitution of one or more bases and has the same binding function, and is obtained by screening by an improved Capture-SELEX method, which introduces progressive counter-screening strategy in screening process, effectively improves the specificity of aptamer.The preferred aptamer 34S (SEQ ID NO:3) provided by the application is verified by isothermal titration calorimetry, and has nanomolar level affinity with meso-diaminopimelic acid (DAP).The aptamer can be used as a core recognition element to construct electrochemical, optical and other types of biosensors, detection kits, rapid detection systems or equipment, to realize rapid, sensitive and specific detection of DAP, and has important application value in the field of bacterial identification, infectious disease identification and the like.

The invention provides a laboratory strain AI identification method and system based on YOLOv8. The method comprises the following steps: S1, carrying out laboratory detection on data acquisition of related strains; s2, cleaning, labeling, cutting, grouping and preprocessing the collected data set; s3, performing model training and algorithm adjustment on the processed data based on YOLOv8; s4, according to the trained data, establishing a basic strain identification reasoning model, and performing reasoning; s5, after reasoning is completed, the strain identification system automatically displays an identification result and pushes the identification result to a business system; by adopting a YOLOv8-based advanced visual model structure and combining a self-developed calculation algorithm, the problems of low automation degree, insufficient recognition accuracy and low classification and counting efficiency in a laboratory strain recognition process are solved.

Provided are antibacterial phages that selectively kill bacteria having a resistance gene or the like. Antibacterial phage for this includes CRISPR-Cas13a with a target sequence that recognizes a specific gene as a target. This target sequence is designed as a spacer sequence for crRNA of 14-28 bases. Specific genes are drug resistance genes and toxins. The drug resistance genes are included in bacterial genomes and / or plasmids having one or any combination of the group including: methicillin-resistant Staphylococcus aureus, vancomycin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, penicillin-resistant pneumococcus, multidrug-resistant Pseudomonas aeruginosa, multidrug-resistant Pseudomonas aeruginosa, carbapenem-resistant Pseudomonas aeruginosa, carbapenem-resistant cephalosporins, third-generation cephalosporin-resistant Pseudomonas aeruginosa, third-generation cephalosporin-resistant E. coli, and fluoroquinolone-resistant E. coli.

The utility model discloses a full-automatic omnibearing bacteria identification and drug sensitivity tester, which comprises a microorganism drug sensitivity analyzer and a bearing block, the bearing block is fixedly connected with a damping block, the lower surface of the microorganism drug sensitivity analyzer is fixedly connected with a connecting block, and the inner surface of the bearing block is fixedly connected with a plurality of bearing rods. Through the arrangement of rolling wheels, telescopic air cylinders, anti-skid pads and other structures, when the whole equipment needs to be moved into a room, fixing of the equipment is relieved firstly, then the equipment is pushed, and therefore under the action of the rolling wheels, the whole equipment can be moved, a manual carrying mode does not need to be adopted any more, the labor intensity of workers is relieved, and waste of human resources is reduced; and after the equipment moves to a proper position, a connecting plate, a fixing block and an anti-skid pad make contact with the ground by starting a telescopic air cylinder, then the position of the device can be fixed, and under the action of the anti-skid pad, the stability of the device after fixing can be improved.

The invention belongs to the field of bacteria identification, and particularly relates to a method for rapidly and nondestructively identifying compound bacteria. The method comprises the following steps: culturing single bacterial colonies, respectively measuring the OD600nm absorbance value of bacterial liquid after the culture is completed, and then diluting the bacterial liquid until the OD600nm is equal to 0.05 to obtain diluted bacterial liquid; adsorbing the diluted bacterial liquid by adopting solid-phase microextraction; after gas chromatography and mass spectrometry detection, principal component analysis and partial least squares discrimination / OPLS-DA analysis are carried out on a bacterial VOCs metabolic spectrum to determine different VOCs of different bacteria, so that different bacteria are discriminated. According to the method, the volatile organic compounds of the bacteria are detected by adopting a solid-phase microextraction-gas phase mass spectrometry technology, and a method for non-destructive detection and rapid identification of four different bacteria and drug-resistant pseudomonas aeruginosa is established by combining a multivariate statistical analysis method, so that the method has the advantages of short detection time, no damage to a sample, high specificity and the like.

The invention discloses a nucleic acid aptamer specifically combined with meso-diaminopimelic acid and application of the nucleic acid aptamer, and belongs to the technical field of bioengineering and detection. The sequence of the nucleic acid aptamer is shown as SEQ ID NO: 2, or a variant which is obtained by adding, deleting or replacing one or more basic groups on the sequence and has the same binding function, the nucleic acid aptamer is obtained by screening through an improved Capture-SELEX method, a progressive reverse screening strategy is introduced in the screening process, and the specificity of the aptamer is effectively improved. The optimal aptamer 34S (SEQ ID NO: 3) provided by the invention is verified by an isothermal titration calorimetry, and the optimal aptamer 34S (SEQ ID NO: 3) and meso-diaminopimelic acid (DAP) have nano-mole-level affinity. The aptamer can be used as a core recognition element, is used for constructing electrochemical, optical and other types of biosensors, detection kits, rapid detection systems or equipment, realizes rapid, sensitive and specific detection of DAP, and has important application value in the fields of bacterial identification, infectious disease recognition and the like.

Devices for performing growth detection, identification and antimicrobial susceptibility testing (AST) functions in connection with a microbial species to achieve sample-to-answer results are disclosed. Methods of performing microbial growth, microbial species identification, and AST using such devices are also disclosed.

The invention relates to the technical field of bacterium identification instruments, in particular to a portable bacterium identification instrument and a using method thereof, and the portable bacterium identification instrument comprises a shell, a flip cover, a detection circuit board, a rotating disc, a mounting column, a rotating column and a detection assembly; the shell is in a cuboid shape, the interior of the shell is of a hollow structure, the flip cover is arranged on the upper face of the shell, the detection circuit board is installed in the shell, the rotating disc is installed beside the detection circuit board and can rotate around the center point of the rotating disc, and the installation column is installed at the center of the upper face of the rotating disc and can rotate around the center point of the rotating disc. The rotating column is mounted on the outer side of the mounting column, and the detection assembly is mounted on the mounting column; the detection assembly is driven by the rotating disc to rotate, and the generated centrifugal force can transport the detection liquid of the detection bottom plate into the storage cavity, so that the cleaning work is completed.

The invention discloses a method and a device for separating and culturing microorganisms from a large number of liquid samples, and the method comprises the following steps of: pouring or dropwise adding a quantitative liquid sample to be detected onto the surface of an agar culture medium by using a traditional agar culture medium by using a sterile method; a dry powder spraying instrument designed by the invention is used for spraying sterile polymer dry powder onto an agar culture medium, the polymer is cross-linked to form a three-dimensional structure adsorption layer, microorganisms in a sample are rapidly fixed on the surface of the agar culture medium, the microorganisms of a curing layer are incubated and cultured to form bacterial colonies for detection, and the bacterial colonies can be counted; the bacterial colonies can also be directly applied to further operations such as bacterial identification, drug sensitive test or other microbiological analysis and detection. According to the method, on the basis of a traditional solid culture scheme, a large number of liquid samples are directly subjected to solid treatment, integration of the samples and the agar culture medium is achieved, and the large number of liquid samples can be cultured on the agar culture medium.

Provided are antibacterial phages that selectively kill bacteria having a drug resistance gene or the like. Antibacterial phage for this includes CRISPR-Cas13a with a target sequence that recognizes a specific gene as a target. This target sequence is designed as a spacer sequence for crRNA of 14-28 bases. Specific genes are drug resistance genes and toxins. The drug resistance genes are included in bacterial genomes and / or plasmids having one or any combination of the group including: methicillin-resistant Staphylococcus aureus, vancomycin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, penicillin-resistant pneumococcus, multidrug-resistant Pseudomonas aeruginosa, multidrug-resistant Pseudomonas aeruginosa, carbapenem-resistant Pseudomonas aeruginosa, carbapenem-resistant cephalosporins, third-generation cephalosporin-resistant Pseudomonas aeruginosa, third-generation cephalosporin-resistant E. coli, and fluoroquinolone-resistant E. coli.

A test device for indole concentrations in headspace gasses of bacterial cultures has a porous substrate imprinted with a wax barrier surrounding a test spot impregnated with p-dimethylaminocinnamaldehyde (DMACA). In embodiments, the test spot lies within a printed bar code and is configured to alter a reading of the bar code when the test spot darkens. The test device may optionally include a second test spot impregnated with Bromthymol Blue, Cobinamide, p-dimethylaminobenzaldehyde, or Chromotropic acid. The device is used by inoculating a sample into a culture; incubating the culture; inserting the test device into airspace of the culture, and observing the test spot for a color change indicative of indole presence.

Relates to a test method and test kit for the identification of the type, species or strain of bacteria contained in a sample, where the capture probe contains a bacteriophage receptor binding protein specific for the bacteria to be tested. The receptor binding protein can be labelled in conjugated form with gold nanoparticles.

The utility model relates to the related technical field of laboratory design, in particular to a bacteria identification and analysis laboratory, which comprises a plurality of laboratory main bodies formed by concrete wallboards, and further comprises an experiment table, a sample preparation table, a sample culture table and a sample identification and analysis table which are arranged in the laboratory main bodies and are independently arranged in a partitioned manner; the image acquisition equipment is arranged in the sample identification and analysis table and is used for performing image acquisition in the operation process of bacterial identification and analysis; and the disinfecting and sterilizing mechanism is arranged in the laboratory main body and is used for disinfecting and sterilizing the environment in the laboratory main body and the experiment table. According to the utility model, the disinfecting and killing mechanism is arranged in the laboratory main body, the disinfecting and killing treatment of the disinfecting and killing assembly on the horizontal planes with different heights in the laboratory main body is realized, and the liquid distribution pipeline and the ultraviolet lamp are arranged on the supporting frame together, so that the laboratory environment and the experiment table are well disinfected and killed, manual operation is replaced, and time and labor are saved.

The application belongs to the technical field of bacteria identification, and discloses a multi-dimensional bacteria spectrum acquisition method, a bacteria identification method and an application device, wherein the multi-dimensional bacteria spectrum acquisition method comprises the following steps: cracking a bacteria suspension by using ultrasonic waves to obtain a bacteria lysate, mixing a plurality of surface-enhanced Raman scattering concentrated suspension substrates with the bacteria lysate respectively to obtain a plurality of mixed liquids, forming a liquid film by using the plurality of mixed liquids, performing surface-enhanced Raman scattering analysis on the liquid film, and obtaining multi-dimensional surface-enhanced Raman scattering bacteria spectrum data. The application can acquire multi-dimensional spectrum information of bacteria, and then realizes rapid and accurate detection and identification of bacteria samples.

The invention relates to the technical field of intelligent water cups, and provides an automatic sterilization control method and system for an intelligent water cup, and the method comprises the steps: carrying out the data calibration of ultrasonic data, obtaining the calibration ultrasonic data, carrying out the signal conversion of the calibration ultrasonic data, obtaining an ultrasonic signal, and collecting the signal feature information corresponding to the ultrasonic signal; analyzing ultrasonic scattering characteristics of the ultrasonic waves in the intelligent water cup, determining an initial sound wave intensity value and a feedback sound wave intensity value in the calibration ultrasonic data, and calculating an ultrasonic attenuation coefficient of the ultrasonic waves in the intelligent water cup; the mass density of water in the intelligent water cup is measured, and the ultrasonic reflection coefficient of ultrasonic waves in the intelligent water cup is calculated; performing bacterium identification on the intelligent water cup to obtain a bacterium type, positioning the spatial position of the bacterium type in the intelligent water cup, calculating the bacterium activeness corresponding to the bacterium type, and sterilizing and purifying water in the intelligent water cup to obtain purified water. The sterilization efficiency of the intelligent water cup is improved.

The application discloses a kind of bacterial identification microfluidic chip and use method, including bacterial identification reagent disc, with the center of bacterial identification reagent disc as center, from center to the outside of bacterial identification reagent disc, it is sequentially provided with shaft hole, bacteria liquid sample adding groove, annular flow guide flow channel;Bacteria liquid sample adding groove is arranged outside shaft hole;Annular flow guide flow channel is arranged outside bacteria liquid sample adding groove, and bacteria liquid sample adding groove and annular flow guide flow channel are connected by bending type microfluidic channel;Bacterial identification reaction cavity is arranged outside annular flow guide flow channel, and bacterial identification reaction cavity is communicated with annular flow guide flow channel by bending type microfluidic channel.By the above structural design, the application realizes in improving the efficiency of bacterial identification, while guaranteeing for bacterial identification process in no pollution, identification accuracy, meet the demand of bacterial biochemical property change in bacterial identification process, and the bacterial identification microfluidic chip high applicability, it is easy to operate.

Disclosed herein is a method of detecting an antimicrobial susceptibility of a bacterium to one or more antibiotics, the method requiring the steps of: (a) providing a bacterial mixture that includes a bacterial population including a bacterial species suspected to be resistant to one or more antibiotics, an antibiotic, a metabolic precursor suitable for incorporation into a bacterium, which metabolic precursor is labelled or is capable of being labelled with a detectable moiety, and a nutrient suspension; and (b) aging the bacterial mixture for a period of time, then collecting the bacterial population and resuspending it to provide an antibiotic-challenged bacterial mixture, where a bacterium that is resistant to one or more antibiotics incorporates the metabolic precursor suitable for incorporation into the bacterium.

The invention discloses a standardization method and system for pathogen metagenome background bacterium level identification, and relates to the technical field of clinical pathogenic microorganism detection, the standardization method comprises the following steps: screening core background flora based on metagenome sequencing data; constructing a historical reference library; screening a background bacterium marker; evaluating background bacterium signal intensity; and determining the background bacterium level. According to the standardization method and the judgment system for pathogen metagenome background bacterium level identification, clinical specimen types are not distinguished, a background bacterium judgment method based on the standardized pg amount is introduced, and it is ensured that background bacterium detection amounts of different samples have comparability; and meanwhile, the accuracy of a detection result and the reliability of clinical interpretation can be improved by utilizing a background bacterium identification mechanism with dual-index collaboration, a historical reference library and a dynamic updating mechanism.