6 results about "Bacteria identification" patented technology

The application discloses a kind of nucleic acid aptamer specifically binding meso-diaminopimelic acid and application thereof, belong to the field of bioengineering and detection technology.The sequence of the nucleic acid aptamer is as shown in SEQ ID NO:2, or variant obtained by the addition, deletion or substitution of one or more bases and has the same binding function, and is obtained by screening by an improved Capture-SELEX method, which introduces progressive counter-screening strategy in screening process, effectively improves the specificity of aptamer.The preferred aptamer 34S (SEQ ID NO:3) provided by the application is verified by isothermal titration calorimetry, and has nanomolar level affinity with meso-diaminopimelic acid (DAP).The aptamer can be used as a core recognition element to construct electrochemical, optical and other types of biosensors, detection kits, rapid detection systems or equipment, to realize rapid, sensitive and specific detection of DAP, and has important application value in the field of bacterial identification, infectious disease identification and the like.

Provided are antibacterial phages that selectively kill bacteria having a resistance gene or the like. Antibacterial phage for this includes CRISPR-Cas13a with a target sequence that recognizes a specific gene as a target. This target sequence is designed as a spacer sequence for crRNA of 14-28 bases. Specific genes are drug resistance genes and toxins. The drug resistance genes are included in bacterial genomes and / or plasmids having one or any combination of the group including: methicillin-resistant Staphylococcus aureus, vancomycin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, penicillin-resistant pneumococcus, multidrug-resistant Pseudomonas aeruginosa, multidrug-resistant Pseudomonas aeruginosa, carbapenem-resistant Pseudomonas aeruginosa, carbapenem-resistant cephalosporins, third-generation cephalosporin-resistant Pseudomonas aeruginosa, third-generation cephalosporin-resistant E. coli, and fluoroquinolone-resistant E. coli.

The invention discloses a nucleic acid aptamer specifically combined with meso-diaminopimelic acid and application of the nucleic acid aptamer, and belongs to the technical field of bioengineering and detection. The sequence of the nucleic acid aptamer is shown as SEQ ID NO: 2, or a variant which is obtained by adding, deleting or replacing one or more basic groups on the sequence and has the same binding function, the nucleic acid aptamer is obtained by screening through an improved Capture-SELEX method, a progressive reverse screening strategy is introduced in the screening process, and the specificity of the aptamer is effectively improved. The optimal aptamer 34S (SEQ ID NO: 3) provided by the invention is verified by an isothermal titration calorimetry, and the optimal aptamer 34S (SEQ ID NO: 3) and meso-diaminopimelic acid (DAP) have nano-mole-level affinity. The aptamer can be used as a core recognition element, is used for constructing electrochemical, optical and other types of biosensors, detection kits, rapid detection systems or equipment, realizes rapid, sensitive and specific detection of DAP, and has important application value in the fields of bacterial identification, infectious disease recognition and the like.

Devices for performing growth detection, identification and antimicrobial susceptibility testing (AST) functions in connection with a microbial species to achieve sample-to-answer results are disclosed. Methods of performing microbial growth, microbial species identification, and AST using such devices are also disclosed.

The application discloses a kind of bacterial identification microfluidic chip and use method, including bacterial identification reagent disc, with the center of bacterial identification reagent disc as center, from center to the outside of bacterial identification reagent disc, it is sequentially provided with shaft hole, bacteria liquid sample adding groove, annular flow guide flow channel;Bacteria liquid sample adding groove is arranged outside shaft hole;Annular flow guide flow channel is arranged outside bacteria liquid sample adding groove, and bacteria liquid sample adding groove and annular flow guide flow channel are connected by bending type microfluidic channel;Bacterial identification reaction cavity is arranged outside annular flow guide flow channel, and bacterial identification reaction cavity is communicated with annular flow guide flow channel by bending type microfluidic channel.By the above structural design, the application realizes in improving the efficiency of bacterial identification, while guaranteeing for bacterial identification process in no pollution, identification accuracy, meet the demand of bacterial biochemical property change in bacterial identification process, and the bacterial identification microfluidic chip high applicability, it is easy to operate.

The invention discloses a standardization method and system for pathogen metagenome background bacterium level identification, and relates to the technical field of clinical pathogenic microorganism detection, the standardization method comprises the following steps: screening core background flora based on metagenome sequencing data; constructing a historical reference library; screening a background bacterium marker; evaluating background bacterium signal intensity; and determining the background bacterium level. According to the standardization method and the judgment system for pathogen metagenome background bacterium level identification, clinical specimen types are not distinguished, a background bacterium judgment method based on the standardized pg amount is introduced, and it is ensured that background bacterium detection amounts of different samples have comparability; and meanwhile, the accuracy of a detection result and the reliability of clinical interpretation can be improved by utilizing a background bacterium identification mechanism with dual-index collaboration, a historical reference library and a dynamic updating mechanism.