Pretreatment method for directly detecting infection urine pathogen by MALDI-TOF MS

A technology for pathogenic bacteria and urine, applied in the field of microbiology, can solve problems such as direct identification with less original specimens, and achieve the effects of shortening the measurement time, simplifying the detection steps, and simplifying the detection process.
CN102749233AInactive Publication Date: 2012-10-24鲁辛辛 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
鲁辛辛
Publication Date
2012-10-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a pretreatment method for directly detecting infection urine pathogen by MALDI-TOF MS. An urine sample pretreatment part is improved on a methodology, bacteria is washed and collected, formic acid and acetonitrile are used for performing protein digestion on bacteria, and then the detection is carried out, thereby the bacteria identification time is substantially shortened and the identification accuracy is enhanced. The pretreatment method is capable of rapidly and accurately identifying the infection urine sample, and can timely guide the clinical medication with pertinence.
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Description

technical field

[0001] The invention relates to the detection of pathogenic microorganisms in clinical specimens and belongs to the field of microbiology. Background technique

[0002] Urinary tract infections (UTIs) occupy the second place in the clinical infection rate, and the traditional method (urine culture and identification) takes a long time, and clinicians often do not wait for the results of the laboratory, but choose broad-spectrum antibiotics for treatment. This has caused the drug resistance rate of clinical bacteria to increase year by year.

[0003] The diagnosis of UTIs infection is mainly based on clinical symptoms and laboratory results. The patient should wash the vulva before taking the specimen, and take the midstream urine in a sterile container. The main laboratory tests include: urine dry chemical examination, urine sediment analysis, smear microscopy, Gram stain microscopy, and urine culture identification. Urine culture identification is now con...

Claims

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