The invention provides a standard sample of red sea bream iridobirus
virus molecules and a preparation method for the standard sample. The preparation method specifically comprises the steps: selection of raw materials of RSIV (red sea bream iridobirus
virus), preparation of the standard sample, checking of uniformity and stability, qualitative
verification, value fixing and the like. The preparation method for the standard sample comprises the steps: extracting
a DNA (deoxyribonucleic acid) of a
virus from an RSIV solution, performing PCR (
polymerase chain reaction) to multiply and purify a
target gene fragment, connecting and converting the target fragment, and recycling plasmids. The standard sample of red sea bream iridobirus viruses is high in stability and high in uniformity, supplies requirements of the standard sample on detection researches, medicinal researches and application researches on the red sea bream iridobirus viruses, technically instructs a inspection and
quarantine institution and technically supports service export enterprises. According to the standard sample disclosed by the invention, comparison of different
laboratory results can be realized, so that the
quality control in a laboratory is guaranteed.