Screening method of salt-tolerant growth-promoting rhizosphere bacteria, strain and application of strain

Abstract

The invention provides a screening method of salt-tolerant growth-promoting rhizosphere bacteria, a strain and application of the strain. The screening method comprises the following steps: collecting soil attached to the surfaces of root systems of different types of saline-alkali soil plants as a separation source; diluting the obtained separation source, respectively placing the diluted separation source in LB solid culture media with different salinity concentrations for coating culture, and selecting monoclonal bacteria; carrying out secondary screening on the obtained multiple primary screening rhizosphere bacterial colonies to obtain rhizosphere bacterial single colonies; carrying out bacterial identification on the bacterial colonies to obtain various strains of different types; performing a germination rate test on multiple different types of bacteria, and screening out strains capable of improving the germination rate of the seeds; carrying out further salt tolerance screening on the screened strains, and selecting strains with good salt tolerance; and carrying out growth-promoting function screening on the strains with good salt tolerance, and finally screening out the strains with the growth-promoting function. According to the method, the salt-tolerant rhizosphere growth-promoting bacteria are screened out from the soil attached to the surfaces of the plant root systems of the saline-alkali soil, and the growth condition of crops in the saline-alkali soil can be improved.

Description

Technical field [0001] The present invention is an agricultural - technical field of environmental microbiology, more particularly, to a method for screening of rhizosphere salt growth-promoting bacteria strains and Applications. Background technique [0002] Around plant roots with a thin layer of soil, it is a very important regional roots metabolism, known as the rhizosphere. It refers to the root rhizosphere soil around the plant root growth and activity and the impact of physical, chemical, or biological characteristics by the change. There are a lot rhizosphere microorganisms, such as bacteria, fungi, protozoa and algae, where the most abundant bacteria. Plant to increase pressure on the survival of microorganisms through allelopathy to select their greatest contribution to the growth of bacteria. Because bacteria are the most abundant in the rhizosphere microorganisms, especially considering their competitiveness in root colonization, largely affects the physiology of plan...

AI Technical Summary

Problems solved by technology

However, based on the saline-alkali farmland soil in Xinjiang, there are few types of rhizosphere growth-promoting bacteria found so far. At the same time, considering the biological characteristics of microorganisms themselves, how to screen out salt-tolerant rhizosphere growth-promoting bacteria is very important for Xinjiang saline-alkali soil. Plant growth and ecological reconstruction are of great significance

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